Human Gene ACSF2 (ENST00000300441.9_7) from GENCODE V47lift37
  Description: acyl-CoA synthetase family member 2, transcript variant 2 (from RefSeq NM_025149.6)
Gencode Transcript: ENST00000300441.9_7
Gencode Gene: ENSG00000167107.13_11
Transcript (Including UTRs)
   Position: hg19 chr17:48,503,579-48,552,198 Size: 48,620 Total Exon Count: 16 Strand: +
Coding Region
   Position: hg19 chr17:48,503,623-48,551,913 Size: 48,291 Coding Exon Count: 16 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr17:48,503,579-48,552,198)mRNA (may differ from genome)Protein (615 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsHGNC
MGIOMIMPubMedReactomeUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: ACSF2_HUMAN
DESCRIPTION: RecName: Full=Acyl-CoA synthetase family member 2, mitochondrial; EC=6.2.1.-; Flags: Precursor;
FUNCTION: Acyl-CoA synthases catalyze the initial reaction in fatty acid metabolism, by forming a thioester with CoA. Has some preference toward medium-chain substrates. Plays a role in adipodyte differentiation.
SUBCELLULAR LOCATION: Mitochondrion (Potential).
INDUCTION: By PPARG.
SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 51.77 RPKM in Adrenal Gland
Total median expression: 508.82 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -8.1044-0.184 Picture PostScript Text
3' UTR -92.30285-0.324 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR020845 - AMP-binding_CS
IPR000873 - AMP-dep_Synth/Lig
IPR025110 - DUF4009

Pfam Domains:
PF00501 - AMP-binding enzyme
PF13193 - AMP-binding enzyme C-terminal domain

SCOP Domains:
56801 - Acetyl-CoA synthetase-like

ModBase Predicted Comparative 3D Structure on Q96CM8
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0003824 catalytic activity
GO:0003996 acyl-CoA ligase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016874 ligase activity

Biological Process:
GO:0006629 lipid metabolic process
GO:0006631 fatty acid metabolic process
GO:0006637 acyl-CoA metabolic process
GO:0008152 metabolic process

Cellular Component:
GO:0005739 mitochondrion
GO:0005759 mitochondrial matrix


-  Descriptions from all associated GenBank mRNAs
  AK300625 - Homo sapiens cDNA FLJ50687 complete cds, weakly similar to Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3).
AK294205 - Homo sapiens cDNA FLJ51819 complete cds, weakly similar to Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3).
AY358660 - Homo sapiens clone DNA57129 AVYV493 (UNQ493) mRNA, complete cds.
BC014123 - Homo sapiens acyl-CoA synthetase family member 2, mRNA (cDNA clone MGC:20916 IMAGE:4560029), complete cds.
BC012053 - Homo sapiens acyl-CoA synthetase family member 2, mRNA (cDNA clone MGC:19867 IMAGE:4577089), complete cds.
AK300145 - Homo sapiens cDNA FLJ50666 complete cds, weakly similar to Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3).
AK225561 - Homo sapiens mRNA for hypothetical protein LOC80221 variant, clone: KDN03583.
AK024573 - Homo sapiens cDNA: FLJ20920 fis, clone ADSE00877.
AK225044 - Homo sapiens mRNA for hypothetical protein LOC80221 variant, clone: adSE00877.
AK295258 - Homo sapiens cDNA FLJ54351 complete cds, weakly similar to Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3).
CU680180 - Synthetic construct Homo sapiens gateway clone IMAGE:100018372 5' read FLJ20920 mRNA.
CU680788 - Synthetic construct Homo sapiens gateway clone IMAGE:100017432 5' read FLJ20920 mRNA.
KJ899622 - Synthetic construct Homo sapiens clone ccsbBroadEn_09016 ACSF2 gene, encodes complete protein.
KJ899623 - Synthetic construct Homo sapiens clone ccsbBroadEn_09017 ACSF2 gene, encodes complete protein.
AK311109 - Homo sapiens cDNA, FLJ18151.
AK308584 - Homo sapiens cDNA, FLJ98625.
JD449609 - Sequence 430633 from Patent EP1572962.
JD136398 - Sequence 117422 from Patent EP1572962.
JD161052 - Sequence 142076 from Patent EP1572962.
JD321327 - Sequence 302351 from Patent EP1572962.
JD236843 - Sequence 217867 from Patent EP1572962.
JD044764 - Sequence 25788 from Patent EP1572962.
JD548451 - Sequence 529475 from Patent EP1572962.
JD184058 - Sequence 165082 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q96CM8 (Reactome details) participates in the following event(s):

R-HSA-5696004 ACSF2 ligates CoA-SH to MCFA
R-HSA-77289 Mitochondrial Fatty Acid Beta-Oxidation
R-HSA-8978868 Fatty acid metabolism
R-HSA-556833 Metabolism of lipids
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: ACSF2 , ACSF2_HUMAN, B4DFQ6, B4DHT5, B4DUF5, ENST00000300441.1, ENST00000300441.2, ENST00000300441.3, ENST00000300441.4, ENST00000300441.5, ENST00000300441.6, ENST00000300441.7, ENST00000300441.8, NM_025149, Q96CM8, Q9H7G2, uc317mpn.1, uc317mpn.2, UNQ493/PRO1009
UCSC ID: ENST00000300441.9_7
RefSeq Accession: NM_025149.6
Protein: Q96CM8 (aka ACSF2_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.