ID:ASAP1_HUMAN DESCRIPTION: RecName: Full=Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1; AltName: Full=130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein; AltName: Full=ADP-ribosylation factor-directed GTPase-activating protein 1; Short=ARF GTPase-activating protein 1; AltName: Full=Development and differentiation-enhancing factor 1; Short=DEF-1; Short=Differentiation-enhancing factor 1; AltName: Full=PIP2-dependent ARF1 GAP; FUNCTION: Possesses phosphatidylinositol 4,5-bisphosphate- dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types (By similarity). Plays a role in ciliogenesis. ENZYME REGULATION: Activity stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) (By similarity). SUBUNIT: Homodimer. Interacts with SRC and CRK. Interacts with RAB11FIP3. Interacts with PTK2B/PYK2 (By similarity). Interacts with CTTN. Interacts (via SH3 domain) with APC. INTERACTION: P46108:CRK; NbExp=2; IntAct=EBI-346622, EBI-886; P06241:FYN; NbExp=2; IntAct=EBI-346622, EBI-515315; P62993:GRB2; NbExp=7; IntAct=EBI-346622, EBI-401755; P16333:NCK1; NbExp=5; IntAct=EBI-346622, EBI-389883; P19174:PLCG1; NbExp=3; IntAct=EBI-346622, EBI-79387; P12931:SRC; NbExp=2; IntAct=EBI-346622, EBI-621482; SUBCELLULAR LOCATION: Cytoplasm (By similarity). Membrane (By similarity). Note=Predominantly cytoplasmic (By similarity). Partially membrane-associated (By similarity). DOMAIN: The PH domain most probably contributes to the phosphoinositide-dependent regulation of ADP ribosylation factors (By similarity). PTM: Phosphorylated on tyrosine residues by SRC (By similarity). SIMILARITY: Contains 2 ANK repeats. SIMILARITY: Contains 1 Arf-GAP domain. SIMILARITY: Contains 1 PH domain. SIMILARITY: Contains 1 SH3 domain.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q9ULH1
Front
Top
Side
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.
Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
AB033075 - Homo sapiens mRNA for KIAA1249 protein, partial cds. BX537768 - Homo sapiens mRNA; cDNA DKFZp781K0428 (from clone DKFZp781K0428). BC137135 - Homo sapiens ArfGAP with SH3 domain, ankyrin repeat and PH domain 1, mRNA (cDNA clone MGC:168755 IMAGE:9021132), complete cds. BC144493 - Homo sapiens cDNA clone IMAGE:9053020. AX721314 - Sequence 274 from Patent WO0220754. BC070268 - Homo sapiens cDNA clone IMAGE:3997001, partial cds. BC020631 - Homo sapiens ArfGAP with SH3 domain, ankyrin repeat and PH domain 1, mRNA (cDNA clone IMAGE:4733268), complete cds. AK056811 - Homo sapiens cDNA FLJ32249 fis, clone PROST1000144. BC105978 - Homo sapiens cDNA clone IMAGE:4444315. AF222859 - Homo sapiens uncharacterized gastric protein ZG14P mRNA, complete cds. AK314695 - Homo sapiens cDNA, FLJ95549. HQ257918 - Synthetic construct Homo sapiens clone IMAGE:100072227 Unknown protein gene, encodes complete protein. JD148418 - Sequence 129442 from Patent EP1572962. DQ591419 - Homo sapiens piRNA piR-58531, complete sequence.
Biochemical and Signaling Pathways
BioCarta from NCI Cancer Genome Anatomy Project h_arapPathway - ADP-Ribosylation Factor
Reactome (by CSHL, EBI, and GO)
Protein Q9ULH1 (Reactome details) participates in the following event(s):
R-HSA-5620918 ASAP1 dimer binds membrane proteins R-HSA-5620921 ASAP1 recruits RAB11FIP3 and RAB11 to the ciliary targeting complex R-HSA-5623519 RAB3IP and RAB8A bind to the ciliary targeting complex R-HSA-5623513 ASAP1 stimulates GTPase activity of ARF4 R-HSA-5623521 RAB3IP stimulates nucleotide exchange on RAB8A R-HSA-5623524 The exocyst complex binds to RAB3IP in the ciliary targeting complex R-HSA-5620916 VxPx cargo-targeting to cilium R-HSA-5620920 Cargo trafficking to the periciliary membrane R-HSA-5617833 Cilium Assembly R-HSA-1852241 Organelle biogenesis and maintenance
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
