ID:MPIP3_HUMAN DESCRIPTION: RecName: Full=M-phase inducer phosphatase 3; EC=3.1.3.48; AltName: Full=Dual specificity phosphatase Cdc25C; FUNCTION: Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity. CATALYTIC ACTIVITY: Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate. SUBUNIT: Interacts with HIV-1 Vpr, thereby inactivating CDC25C phosphatase activity. Interacts with MAPK14 and 14-3-3 proteins. When phosphorylated on Ser-129 and/or Thr-130, interacts with PLK1. INTERACTION: P06493:CDK1; NbExp=2; IntAct=EBI-974439, EBI-444308; O14757:CHEK1; NbExp=2; IntAct=EBI-974439, EBI-974488; Q9Y250:LZTS1; NbExp=2; IntAct=EBI-974439, EBI-1216080; SUBCELLULAR LOCATION: Nucleus. DEVELOPMENTAL STAGE: Expressed predominantly in G2 phase. PTM: Phosphorylated by CHEK1 and MAPK14 at Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4. Phosphorylated by PLK1, leading to activate the phosphatase activity. Phosphorylation by PLK3 at Ser-191 promotes nuclear translocation. Ser-198 is a minor phosphorylation site. Was initially reported to be phosphorylated by PLK3 at Ser-216 (PubMed:10557092). However, such phosphorylation by PLK3 was not confirmed by other groups. Phosphorylation at Thr-48, Thr-67, Ser-122, Thr-130, Ser-168 and Ser-214 occurs at G2 and G2-M transition and is probably catalyzed by CDK1. Ser-168 phosphorylation levels are lower than those at the other 5 CDK1 sites. Phosphorylation by CDK1 leads to increased activity. SIMILARITY: Belongs to the MPI phosphatase family. SIMILARITY: Contains 1 rhodanese domain. WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/cdc25c/";
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on P30307
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
M34065 - Human cdc25Hs mRNA, complete cds. LF385247 - JP 2014500723-A/192750: Polycomb-Associated Non-Coding RNAs. BC019089 - Homo sapiens cell division cycle 25 homolog C (S. pombe), mRNA (cDNA clone MGC:29447 IMAGE:4858949), complete cds. AK301828 - Homo sapiens cDNA FLJ56262 complete cds, highly similar to M-phase inducer phosphatase 3 (EC 3.1.3.48). AK097710 - Homo sapiens cDNA FLJ40391 fis, clone TESTI2036833, highly similar to M-PHASE INDUCER PHOSPHATASE 3 (EC 3.1.3.48). AJ304504 - Homo sapiens mRNA for M-phase inducer phosphatase 3 isoform cdc25Cdm, (CDC25C gene). EU832624 - Synthetic construct Homo sapiens clone HAIB:100067653; DKFZo008B1231 cell division cycle 25 homolog C (S. pombe) protein (CDC25C) gene, encodes complete protein. KJ896578 - Synthetic construct Homo sapiens clone ccsbBroadEn_05972 CDC25C gene, encodes complete protein. EU832697 - Synthetic construct Homo sapiens clone HAIB:100067726; DKFZo004B1232 cell division cycle 25 homolog C (S. pombe) protein (CDC25C) gene, encodes complete protein. MA620824 - JP 2018138019-A/192750: Polycomb-Associated Non-Coding RNAs. JD491490 - Sequence 472514 from Patent EP1572962. JD251894 - Sequence 232918 from Patent EP1572962. JD474439 - Sequence 455463 from Patent EP1572962. JD368622 - Sequence 349646 from Patent EP1572962. JD419227 - Sequence 400251 from Patent EP1572962. LF343079 - JP 2014500723-A/150582: Polycomb-Associated Non-Coding RNAs. JD513233 - Sequence 494257 from Patent EP1572962. JD334541 - Sequence 315565 from Patent EP1572962. JD517068 - Sequence 498092 from Patent EP1572962. MA578656 - JP 2018138019-A/150582: Polycomb-Associated Non-Coding RNAs. CU676376 - Synthetic construct Homo sapiens gateway clone IMAGE:100019565 5' read CDC25C mRNA. AF312681 - Homo sapiens CDC25C splicing variant 2 mRNA, partial cds. AF277723 - Homo sapiens cell division cycle 25C splice variant 2 (CDC25C) mRNA, partial cds. AF277724 - Homo sapiens cell division cycle 25C splice variant 3 (CDC25C) mRNA, partial cds. AF277725 - Homo sapiens cell division cycle 25C splice variant 4 (CDC25C) mRNA, partial cds. AF277726 - Homo sapiens cell division cycle 25C splice variant 5 (CDC25C) mRNA, partial cds. AF086323 - Homo sapiens full length insert cDNA clone ZD53E04. JD165844 - Sequence 146868 from Patent EP1572962. JD327574 - Sequence 308598 from Patent EP1572962. JD058889 - Sequence 39913 from Patent EP1572962.
Biochemical and Signaling Pathways
BioCarta from NCI Cancer Genome Anatomy Project h_mPRPathway - How Progesterone Initiates the Oocyte Maturation h_cdc25Pathway - cdc25 and chk1 Regulatory Pathway in response to DNA damage h_srcRPTPPathway - Activation of Src by Protein-tyrosine phosphatase alpha h_g2Pathway - Cell Cycle: G2/M Checkpoint h_plk3Pathway - Regulation of cell cycle progression by Plk3 h_ptc1Pathway - Sonic Hedgehog (SHH) Receptor Ptc1 Regulates cell cycle h_rbPathway - RB Tumor Suppressor/Checkpoint Signaling in response to DNA damage
Reactome (by CSHL, EBI, and GO)
Protein P30307 (Reactome details) participates in the following event(s):
R-HSA-75010 Phosphorylation of Cdc25C at Ser 216 by Chk1 R-HSA-75809 Phosphorylation of Cdc25C at Ser216 by CHEK2 R-HSA-6802973 PLK3 phosphorylates CDC25C R-HSA-75016 Association of p-S216-CDC25C with 14-3-3 proteins R-HSA-5671749 p-T774-PKN1 phosphorylates CDC25C R-HSA-170149 Translocation of active Cdc25C to the nucleus R-HSA-156678 Activation of Cdc25C R-HSA-5671737 p-T774-PKN1 binds CDC25C R-HSA-8863011 p25-bound CDK5 phosphorylates CDC25C R-HSA-8863674 CDC25C binds YWHAE (14-3-3-epsilon) R-HSA-170159 Translocation of Cdc25 to the nucleus R-HSA-170153 Dephosphorylation of nuclear Cyclin B1:phospho-Cdc2 (Thr 14, Tyr15) complexes by Cdc25 phosphatases R-HSA-176187 Activation of ATR in response to replication stress R-HSA-75035 Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex R-HSA-6804115 TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain R-HSA-6804114 TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest R-HSA-5625740 RHO GTPases activate PKNs R-HSA-69273 Cyclin A/B1/B2 associated events during G2/M transition R-HSA-156711 Polo-like kinase mediated events R-HSA-8862803 Deregulated CDK5 triggers multiple neurodegenerative pathways in Alzheimer's disease models R-HSA-69481 G2/M Checkpoints R-HSA-69473 G2/M DNA damage checkpoint R-HSA-6791312 TP53 Regulates Transcription of Cell Cycle Genes R-HSA-195258 RHO GTPase Effectors R-HSA-69275 G2/M Transition R-HSA-8863678 Neurodegenerative Diseases R-HSA-69620 Cell Cycle Checkpoints R-HSA-3700989 Transcriptional Regulation by TP53 R-HSA-194315 Signaling by Rho GTPases R-HSA-453274 Mitotic G2-G2/M phases R-HSA-1643685 Disease R-HSA-1640170 Cell Cycle R-HSA-212436 Generic Transcription Pathway R-HSA-162582 Signal Transduction R-HSA-69278 Cell Cycle (Mitotic) R-HSA-73857 RNA Polymerase II Transcription R-HSA-74160 Gene expression (Transcription)
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
