Human Gene CHRM3 (ENST00000676153.1_3) from GENCODE V47lift37
  Description: cholinergic receptor muscarinic 3, transcript variant 10 (from RefSeq NM_001375985.1)
Gencode Transcript: ENST00000676153.1_3
Gencode Gene: ENSG00000133019.12_14
Transcript (Including UTRs)
   Position: hg19 chr1:239,549,868-240,078,750 Size: 528,883 Total Exon Count: 7 Strand: +
Coding Region
   Position: hg19 chr1:240,070,752-240,072,524 Size: 1,773 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:239,549,868-240,078,750)mRNA (may differ from genome)Protein (590 aa)
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UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: ACM3_HUMAN
DESCRIPTION: RecName: Full=Muscarinic acetylcholine receptor M3;
FUNCTION: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein.
DISEASE: Defects in CHRM3 are the cause of Eagle-Barrett syndrome (EGBRS) [MIM:100100]. EGBRS is a syndrome characterized by thin abdominal musculature with overlying lax skin, cryptorchism, megacystis with disorganized detrusor muscle, and urinary tract abnormalities.
SIMILARITY: Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: CHRM3
Diseases sorted by gene-association score: prune belly syndrome* (1419), prune belly syndome* (400), cholinergic urticaria (23), bladder disease (13), aplasia of lacrimal and salivary glands (10), anhidrosis (9), ascending cholangitis (9), sjogren's syndrome (7), fetal alcohol syndrome (6), urofacial syndrome 1 (6), ureterolithiasis (6), pyelitis (4), pulmonary disease, chronic obstructive (4), autistic disorder (1)
* = Manually curated disease association

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -463.301180-0.393 Picture PostScript Text
3' UTR -1641.206226-0.264 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000276 - 7TM_GPCR_Rhodpsn
IPR017452 - GPCR_Rhodpsn_supfam
IPR001183 - Musac_M3_rcpt
IPR000995 - Musac_rcpt

Pfam Domains:
PF00001 - 7 transmembrane receptor (rhodopsin family)
PF10320 - Serpentine type 7TM GPCR chemoreceptor Srsx

SCOP Domains:
81321 - Family A G protein-coupled receptor-like

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2CSA - NMR


ModBase Predicted Comparative 3D Structure on P20309
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004435 phosphatidylinositol phospholipase C activity
GO:0004871 signal transducer activity
GO:0004930 G-protein coupled receptor activity
GO:0005515 protein binding
GO:0008144 drug binding
GO:0016907 G-protein coupled acetylcholine receptor activity
GO:0038023 signaling receptor activity
GO:0042166 acetylcholine binding

Biological Process:
GO:0003056 regulation of vascular smooth muscle contraction
GO:0006464 cellular protein modification process
GO:0006939 smooth muscle contraction
GO:0007165 signal transduction
GO:0007186 G-protein coupled receptor signaling pathway
GO:0007197 adenylate cyclase-inhibiting G-protein coupled acetylcholine receptor signaling pathway
GO:0007207 phospholipase C-activating G-protein coupled acetylcholine receptor signaling pathway
GO:0007213 G-protein coupled acetylcholine receptor signaling pathway
GO:0007271 synaptic transmission, cholinergic
GO:0007399 nervous system development
GO:0008283 cell proliferation
GO:0019229 regulation of vasoconstriction
GO:0045987 positive regulation of smooth muscle contraction
GO:0046541 saliva secretion

Cellular Component:
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0016323 basolateral plasma membrane
GO:0030054 cell junction
GO:0030425 dendrite
GO:0032279 asymmetric synapse
GO:0043679 axon terminus
GO:0045202 synapse
GO:0045211 postsynaptic membrane


-  Descriptions from all associated GenBank mRNAs
  AK056349 - Homo sapiens cDNA FLJ31787 fis, clone NT2RI2008566.
AF279779 - Homo sapiens clone N10 NTera2D1 teratocarcinoma, m3 muscarinic acetylcholine receptor mRNA, partial cds.
BC121026 - Homo sapiens cholinergic receptor, muscarinic 3, mRNA (cDNA clone MGC:149689 IMAGE:40117429), complete cds.
AK074575 - Homo sapiens cDNA FLJ90094 fis, clone HEMBA1005545, highly similar to MUSCARINIC ACETYLCHOLINE RECEPTOR M3.
EU832135 - Synthetic construct Homo sapiens clone HAIB:100067164; DKFZo008E0125 cholinergic receptor, muscarinic 3 protein (CHRM3) gene, encodes complete protein.
EU832228 - Synthetic construct Homo sapiens clone HAIB:100067257; DKFZo004E0126 cholinergic receptor, muscarinic 3 protein (CHRM3) gene, encodes complete protein.
KJ890911 - Synthetic construct Homo sapiens clone ccsbBroadEn_00305 CHRM3 gene, encodes complete protein.
AF385589 - Homo sapiens m3 muscarinic cholinergic receptor (CHRM3) mRNA, partial cds.
AB587370 - Synthetic construct DNA, clone: pF1KB3755, Homo sapiens CHRM3 gene for cholinergic receptor, muscarinic 3, without stop codon, in Flexi system.
BC096844 - Homo sapiens cholinergic receptor, muscarinic 3, mRNA (cDNA clone IMAGE:6519222), partial cds.
AF498917 - Homo sapiens muscarinic acetylcholine receptor M3 (CHRM3) mRNA, complete cds.
JD103329 - Sequence 84353 from Patent EP1572962.
JD173175 - Sequence 154199 from Patent EP1572962.
AK095715 - Homo sapiens cDNA FLJ38396 fis, clone FEBRA2007957.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein P20309 (Reactome details) participates in the following event(s):

R-HSA-400012 Acetylcholine binds Muscarinic Acetylcholine Receptor M3
R-HSA-390649 M1, M3 and M5 receptors bind acetylcholine
R-HSA-399995 Muscarinic Acetylcholine Receptor M3 activates Gq
R-HSA-749452 The Ligand:GPCR:Gq complex dissociates
R-HSA-749448 Liganded Gq-activating GPCRs bind inactive heterotrimeric Gq
R-HSA-379048 Liganded Gq/11-activating GPCRs act as GEFs for Gq/11
R-HSA-399997 Acetylcholine regulates insulin secretion
R-HSA-390648 Muscarinic acetylcholine receptors
R-HSA-422356 Regulation of insulin secretion
R-HSA-416476 G alpha (q) signalling events
R-HSA-375280 Amine ligand-binding receptors
R-HSA-163685 Energy Metabolism
R-HSA-388396 GPCR downstream signalling
R-HSA-373076 Class A/1 (Rhodopsin-like receptors)
R-HSA-1430728 Metabolism
R-HSA-372790 Signaling by GPCR
R-HSA-500792 GPCR ligand binding
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: ACM3_HUMAN, NM_001375985, P20309, Q0VAJ8, Q4QRI3, Q5VXY2, Q9HB60, uc330atg.1, uc330atg.2
UCSC ID: ENST00000676153.1_3
RefSeq Accession: NM_001375978.1
Protein: P20309 (aka ACM3_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.