Human Gene DCAF6 (ENST00000367840.4_9) from GENCODE V47lift37
  Description: DDB1 and CUL4 associated factor 6, transcript variant 17 (from RefSeq NM_001393651.1)
Gencode Transcript: ENST00000367840.4_9
Gencode Gene: ENSG00000143164.16_12
Transcript (Including UTRs)
   Position: hg19 chr1:167,905,921-168,045,074 Size: 139,154 Total Exon Count: 22 Strand: +
Coding Region
   Position: hg19 chr1:167,906,150-168,044,673 Size: 138,524 Coding Exon Count: 22 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:167,905,921-168,045,074)mRNA (may differ from genome)Protein (951 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblExonPrimerGeneCardsHGNC
MalacardsMGIPubMedReactomeUniProtKB

-  Comments and Description Text from UniProtKB
  ID: DCAF6_HUMAN
DESCRIPTION: RecName: Full=DDB1- and CUL4-associated factor 6; AltName: Full=Androgen receptor complex-associated protein; Short=ARCAP; AltName: Full=IQ motif and WD repeat-containing protein 1; AltName: Full=Nuclear receptor interaction protein; Short=NRIP;
FUNCTION: Ligand-dependent coactivator of nuclear receptors. Enhance transcriptional activity of the nuclear receptors NR3C1 and AR. May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.
PATHWAY: Protein modification; protein ubiquitination.
SUBUNIT: Interacts with the nuclear receptors NR3C1 and AR in the presence of ligand. Interacts with DDB1, CUL4A and CUL4B.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Highly expressed in skeletal muscle and testis. Expressed to a lesser degree in heart, prostate, and adrenal gland.
SIMILARITY: Contains 1 IQ domain.
SIMILARITY: Contains 7 WD repeats.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: DCAF6
Diseases sorted by gene-association score: male reproductive organ cancer (1)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 122.98 RPKM in Muscle - Skeletal
Total median expression: 926.96 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -99.20229-0.433 Picture PostScript Text
3' UTR -83.60401-0.208 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000048 - IQ_motif_EF-hand-BS
IPR015943 - WD40/YVTN_repeat-like_dom
IPR001680 - WD40_repeat
IPR017986 - WD40_repeat_dom

Pfam Domains:
PF00400 - WD domain, G-beta repeat

SCOP Domains:
50952 - Soluble quinoprotein glucose dehydrogenase
50960 - TolB, C-terminal domain
63825 - YWTD domain
63829 - Calcium-dependent phosphotriesterase
69304 - Tricorn protease N-terminal domain
101898 - NHL repeat
50965 - Galactose oxidase, central domain
75011 - 3-carboxy-cis,cis-mucoante lactonizing enzyme
101908 - Putative isomerase YbhE
117289 - Nucleoporin domain
50969 - YVTN repeat-like/Quinoprotein amine dehydrogenase
50974 - Nitrous oxide reductase, N-terminal domain
50978 - WD40 repeat-like
50993 - Peptidase/esterase 'gauge' domain
69322 - Tricorn protease domain 2
50998 - Quinoprotein alcohol dehydrogenase-like
51004 - C-terminal (heme d1) domain of cytochrome cd1-nitrite reductase
82171 - DPP6 N-terminal domain-like

Protein Data Bank (PDB) 3-D Structure
MuPIT help
3I7O - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q58WW2
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding
GO:0030374 ligand-dependent nuclear receptor transcription coactivator activity

Biological Process:
GO:0016567 protein ubiquitination
GO:0043687 post-translational protein modification
GO:0045944 positive regulation of transcription from RNA polymerase II promoter

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005829 cytosol
GO:0005925 focal adhesion
GO:0080008 Cul4-RING E3 ubiquitin ligase complex


-  Descriptions from all associated GenBank mRNAs
  LF383655 - JP 2014500723-A/191158: Polycomb-Associated Non-Coding RNAs.
AK297847 - Homo sapiens cDNA FLJ58595 complete cds, highly similar to Homo sapiens IQ motif and WD repeats 1 (IQWD1), transcript variant 1, mRNA.
AK226181 - Homo sapiens mRNA for IQ motif and WD repeats 1 isoform b variant, clone: ek00226.
AF116725 - Homo sapiens MSTP055 mRNA, complete cds.
AY766164 - Homo sapiens nuclear receptor interaction protein (NRIP) mRNA, complete cds.
DQ768089 - Homo sapiens androgen receptor complex-associated protein (ARCAP) mRNA, complete cds.
AB385156 - Synthetic construct DNA, clone: pF1KB5691, Homo sapiens IQWD1 gene for IQ motif and WD repeats 1 isoform b, complete cds, without stop codon, in Flexi system.
AF124434 - Homo sapiens HOM-TES-95 tumor antigen mRNA, complete sequence.
AF150734 - Homo sapiens PC326 protein (PC326) mRNA, complete cds.
BC025262 - Homo sapiens IQ motif and WD repeats 1, mRNA (cDNA clone IMAGE:4763336), partial cds.
DQ895337 - Synthetic construct Homo sapiens clone IMAGE:100009797; FLH183393.01L; RZPDo839B11141D IQ motif and WD repeats 1 (IQWD1) gene, encodes complete protein.
DQ892143 - Synthetic construct clone IMAGE:100004773; FLH183397.01X; RZPDo839B11142D IQ motif and WD repeats 1 (IQWD1) gene, encodes complete protein.
AL136738 - Homo sapiens mRNA; cDNA DKFZp434C2211 (from clone DKFZp434C2211).
AK000984 - Homo sapiens cDNA FLJ10122 fis, clone HEMBA1002937.
MA619232 - JP 2018138019-A/191158: Polycomb-Associated Non-Coding RNAs.
AK093970 - Homo sapiens cDNA FLJ36651 fis, clone UTERU2000095, highly similar to Homo sapiens PC326 protein (PC326) mRNA.
AX748387 - Sequence 1912 from Patent EP1308459.
JD231788 - Sequence 212812 from Patent EP1572962.
JD041833 - Sequence 22857 from Patent EP1572962.
JD156856 - Sequence 137880 from Patent EP1572962.
JD313440 - Sequence 294464 from Patent EP1572962.
JD083694 - Sequence 64718 from Patent EP1572962.
JD264378 - Sequence 245402 from Patent EP1572962.
JD048809 - Sequence 29833 from Patent EP1572962.
JD102982 - Sequence 84006 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q58WW2 (Reactome details) participates in the following event(s):

R-HSA-8955245 CAND1 binds CRL4 E3 ubiquitin ligase in the nucleus
R-HSA-8955285 COMMDs displace CAND1 from CRL4 E3 ubiquitin ligase complex
R-HSA-8952639 NEDD8:AcM-UBE2M binds CRL4 E3 ubiquitin ligase complex
R-HSA-8956045 COP9 signalosome deneddylates nuclear CRL4 E3 ubiquitin ligase complex
R-HSA-8952638 AcM-UBE2M transfers NEDD8 to CRL4 E3 ubiquitin ligase complex
R-HSA-8951664 Neddylation
R-HSA-597592 Post-translational protein modification
R-HSA-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: A2A295, B4DNB8, DCAF6_HUMAN, ENST00000367840.1, ENST00000367840.2, ENST00000367840.3, IQWD1, MSTP055, NM_001393651, Q58WW2, Q7L8I0, Q8IXH3, Q8TB19, uc318gga.1, uc318gga.2
UCSC ID: ENST00000367840.4_9
RefSeq Accession: NM_001198956.2
Protein: Q58WW2 (aka DCAF6_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.