Human Gene EIF4ENIF1 (ENST00000330125.10_8) from GENCODE V47lift37
  Description: eukaryotic translation initiation factor 4E nuclear import factor 1, transcript variant 1 (from RefSeq NM_019843.4)
Gencode Transcript: ENST00000330125.10_8
Gencode Gene: ENSG00000184708.18_10
Transcript (Including UTRs)
   Position: hg19 chr22:31,835,353-31,885,828 Size: 50,476 Total Exon Count: 19 Strand: -
Coding Region
   Position: hg19 chr22:31,835,866-31,884,704 Size: 48,839 Coding Exon Count: 18 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr22:31,835,353-31,885,828)mRNA (may differ from genome)Protein (985 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsHGNC
MalacardsMGIOMIMPubMedUniProtKBWikipedia
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: 4ET_HUMAN
DESCRIPTION: RecName: Full=Eukaryotic translation initiation factor 4E transporter; Short=4E-T; Short=eIF4E transporter; AltName: Full=Eukaryotic translation initiation factor 4E nuclear import factor 1;
FUNCTION: Nucleoplasmic shuttling protein. Mediates the nuclear import of EIF4E by a piggy-back mechanism.
SUBUNIT: Interacts with EIF4E. Interacts with importin beta only in the presence of importin alpha, suggesting a direct interaction with importin alpha.
INTERACTION: P06730:EIF4E; NbExp=5; IntAct=EBI-301024, EBI-73440; O60573:EIF4E2; NbExp=3; IntAct=EBI-301024, EBI-398610;
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Nucleus, PML body. Nucleus speckle. Note=Predominantly cytoplasmic. Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner. Localization to nuclear foci and speckles requires active transcription.
TISSUE SPECIFICITY: Widely expressed.
SEQUENCE CAUTION: Sequence=BAB15092.1; Type=Erroneous initiation; Sequence=BAC11194.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: EIF4ENIF1
Diseases sorted by gene-association score: retinitis pigmentosa, y-linked (12)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 29.20 RPKM in Testis
Total median expression: 460.75 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -71.00176-0.403 Picture PostScript Text
3' UTR -148.00513-0.288 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR018862 - eIF4E_transporter

Pfam Domains:
PF10477 - Nucleocytoplasmic shuttling protein for mRNA cap-binding EIF4E

ModBase Predicted Comparative 3D Structure on Q9NRA8
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGDEnsembl   
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003723 RNA binding
GO:0003729 mRNA binding
GO:0005515 protein binding
GO:0008565 protein transporter activity

Biological Process:
GO:0015031 protein transport
GO:0017148 negative regulation of translation
GO:0019827 stem cell population maintenance
GO:0045665 negative regulation of neuron differentiation

Cellular Component:
GO:0000932 P-body
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0016020 membrane
GO:0016605 PML body
GO:0016607 nuclear speck
GO:0043231 intracellular membrane-bounded organelle


-  Descriptions from all associated GenBank mRNAs
  BC032941 - Homo sapiens eukaryotic translation initiation factor 4E nuclear import factor 1, mRNA (cDNA clone MGC:32982 IMAGE:5268671), complete cds.
AK025254 - Homo sapiens cDNA: FLJ21601 fis, clone COL07221.
BC033028 - Homo sapiens eukaryotic translation initiation factor 4E nuclear import factor 1, mRNA (cDNA clone MGC:32981 IMAGE:4837225), complete cds.
AF240775 - Homo sapiens eIF4E-transporter mRNA, complete cds.
AK299136 - Homo sapiens cDNA FLJ58249 complete cds, highly similar to Eukaryotic translation initiation factor 4E transporter.
CR456386 - Homo sapiens bA247I13.2 full length open reading frame (ORF) cDNA clone (cDNA clone C22ORF:pGEM.bA247I13.2).
CU012945 - Homo sapiens EIF4ENIF1, mRNA (cDNA clone IMAGE:100000109), complete cds, with stop codon, in Gateway system.
AK314636 - Homo sapiens cDNA, FLJ95479, highly similar to Homo sapiens eukaryotic translation initiation factor 4E nuclear import factor 1 (EIF4ENIF1), mRNA.
KJ899246 - Synthetic construct Homo sapiens clone ccsbBroadEn_08640 EIF4ENIF1 gene, encodes complete protein.
KJ899247 - Synthetic construct Homo sapiens clone ccsbBroadEn_08641 EIF4ENIF1 gene, encodes complete protein.
DQ892481 - Synthetic construct clone IMAGE:100005111; FLH186708.01X; RZPDo839H0571D eukaryotic translation initiation factor 4E nuclear import factor 1 (EIF4ENIF1) gene, encodes complete protein.
DQ895692 - Synthetic construct Homo sapiens clone IMAGE:100010152; FLH186704.01L; RZPDo839H0561D eukaryotic translation initiation factor 4E nuclear import factor 1 (EIF4ENIF1) gene, encodes complete protein.
CU013233 - Homo sapiens EIF4ENIF1, mRNA (cDNA clone IMAGE:100000013), complete cds, without stop codon, in Gateway system.
AK074768 - Homo sapiens cDNA FLJ90287 fis, clone NT2RP1000903.
AK130061 - Homo sapiens cDNA FLJ26551 fis, clone LNF01609, highly similar to Homo sapiens eukaryotic translation initiation factor 4E nuclear import factor 1 (EIF4ENIF1).
JD072580 - Sequence 53604 from Patent EP1572962.
JD316787 - Sequence 297811 from Patent EP1572962.
JD452006 - Sequence 433030 from Patent EP1572962.
JD141441 - Sequence 122465 from Patent EP1572962.
BC012734 - Homo sapiens eukaryotic translation initiation factor 4E nuclear import factor 1, mRNA (cDNA clone IMAGE:3632537), partial cds.
CU689594 - Synthetic construct Homo sapiens gateway clone IMAGE:100020557 5' read EIF4ENIF1 mRNA.
DQ655937 - Homo sapiens clone UGL2a12, mRNA sequence.
DQ656044 - Homo sapiens clone UGL073-A-G6, mRNA sequence.
JD119605 - Sequence 100629 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: 4ET_HUMAN, B1AKL2, B1AKL3, B2RBF1, EIF4ENIF1 , ENST00000330125.1, ENST00000330125.2, ENST00000330125.3, ENST00000330125.4, ENST00000330125.5, ENST00000330125.6, ENST00000330125.7, ENST00000330125.8, ENST00000330125.9, NM_019843, Q8NCF2, Q9H708, Q9NRA8, uc317swu.1, uc317swu.2
UCSC ID: ENST00000330125.10_8
RefSeq Accession: NM_019843.4
Protein: Q9NRA8 (aka 4ET_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.