Human Gene GGT5 (ENST00000327365.10_7) from GENCODE V47lift37
  Description: gamma-glutamyltransferase 5, transcript variant 2 (from RefSeq NM_004121.5)
Gencode Transcript: ENST00000327365.10_7
Gencode Gene: ENSG00000099998.19_12
Transcript (Including UTRs)
   Position: hg19 chr22:24,615,628-24,641,050 Size: 25,423 Total Exon Count: 12 Strand: -
Coding Region
   Position: hg19 chr22:24,615,938-24,640,693 Size: 24,756 Coding Exon Count: 12 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr22:24,615,628-24,641,050)mRNA (may differ from genome)Protein (586 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsHGNC
MalacardsMGIOMIMPubMedReactomeUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: GGT5_HUMAN
DESCRIPTION: RecName: Full=Gamma-glutamyltransferase 5; Short=GGT 5; EC=2.3.2.2; AltName: Full=Gamma-glutamyl transpeptidase-related enzyme; Short=GGT-rel; AltName: Full=Gamma-glutamyltransferase-like activity 1; AltName: Full=Gamma-glutamyltranspeptidase 5; AltName: Full=Glutathione hydrolase 5; EC=3.4.19.13; AltName: Full=Leukotriene-C4 hydrolase; EC=3.4.19.14; Contains: RecName: Full=Gamma-glutamyltransferase 5 heavy chain; Contains: RecName: Full=Gamma-glutamyltransferase 5 light chain; Flags: Precursor;
FUNCTION: Cleaves the gamma-glutamyl peptide bond of glutathione conjugates, but maybe not glutathione itself. Converts leukotriene C4 (LTC4) to leukotriene D4 (LTD4).
CATALYTIC ACTIVITY: A (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid.
CATALYTIC ACTIVITY: Glutathione + H(2)O = L-cysteinylglycine + L- glutamate.
CATALYTIC ACTIVITY: Leukotriene C(4) + H(2)O = leukotriene D(4) + L-glutamate.
PATHWAY: Sulfur metabolism; glutathione metabolism.
PATHWAY: Lipid metabolism; leukotriene D4 biosynthesis.
SUBUNIT: Heterodimer composed of the light and heavy chains. The active site is located in the light chain (By similarity).
SUBCELLULAR LOCATION: Membrane; Single-pass type II membrane protein (By similarity).
SIMILARITY: Belongs to the gamma-glutamyltransferase family.
SEQUENCE CAUTION: Sequence=CAB55910.1; Type=Frameshift; Positions=446;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: GGT5
Diseases sorted by gene-association score: cataract 4, multiple types (7), fructose-1,6-bisphosphatase deficiency (6)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 72.76 RPKM in Adipose - Subcutaneous
Total median expression: 1137.48 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -69.70357-0.195 Picture PostScript Text
3' UTR -129.90310-0.419 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000101 - GGT_peptidase

Pfam Domains:
PF01019 - Gamma-glutamyltranspeptidase

SCOP Domains:
56235 - N-terminal nucleophile aminohydrolases (Ntn hydrolases)

ModBase Predicted Comparative 3D Structure on P36269
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGDEnsembl   
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000048 peptidyltransferase activity
GO:0008233 peptidase activity
GO:0016740 transferase activity
GO:0016746 transferase activity, transferring acyl groups
GO:0016787 hydrolase activity
GO:0036374 glutathione hydrolase activity

Biological Process:
GO:0006412 translation
GO:0006508 proteolysis
GO:0006520 cellular amino acid metabolic process
GO:0006631 fatty acid metabolic process
GO:0006691 leukotriene metabolic process
GO:0006749 glutathione metabolic process
GO:0006750 glutathione biosynthetic process
GO:0006751 glutathione catabolic process
GO:0006954 inflammatory response
GO:0019370 leukotriene biosynthetic process
GO:1901750 leukotriene D4 biosynthetic process

Cellular Component:
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0031226 intrinsic component of plasma membrane


-  Descriptions from all associated GenBank mRNAs
  AK225661 - Homo sapiens mRNA for Splice isoform 2 of P36269 variant, clone: STM05029.
AK226019 - Homo sapiens mRNA for Splice isoform 2 of P36269 variant, clone: FCC106E04.
AK292006 - Homo sapiens cDNA FLJ75890 complete cds, highly similar to Homo sapiens gamma-glutamyltransferase-like activity 1 (GGTLA1), mRNA.
AK297869 - Homo sapiens cDNA FLJ50588 complete cds, highly similar to Gamma-glutamyltransferase 5 precursor (EC 2.3.2.2).
BC073999 - Homo sapiens gamma-glutamyltransferase 5, mRNA (cDNA clone MGC:87996 IMAGE:6198113), complete cds.
BC011362 - Homo sapiens gamma-glutamyltransferase 5, mRNA (cDNA clone MGC:17034 IMAGE:4179497), complete cds.
AL117414 - Homo sapiens mRNA; cDNA DKFZp566O011 (from clone DKFZp566O011).
M64099 - Human gamma-glutmyl transpeptidase-related protein (GGT-Rel) mRNA, complete cds.
JD381460 - Sequence 362484 from Patent EP1572962.
JD323141 - Sequence 304165 from Patent EP1572962.
JD285945 - Sequence 266969 from Patent EP1572962.
JD142263 - Sequence 123287 from Patent EP1572962.
AK293683 - Homo sapiens cDNA FLJ50454 complete cds, moderately similar to Gamma-glutamyltransferase 5 precursor (EC2.3.2.2).
JD215035 - Sequence 196059 from Patent EP1572962.
JD446749 - Sequence 427773 from Patent EP1572962.
JD539297 - Sequence 520321 from Patent EP1572962.
JD346833 - Sequence 327857 from Patent EP1572962.
CT841518 - Homo sapiens GGTLA1 full length open reading frame (ORF) cDNA clone (cDNA clone C22ORF:pGEM.GGTLA1.V2).
DQ891035 - Synthetic construct clone IMAGE:100003665; FLH168713.01X; RZPDo839C0594D gamma-glutamyltransferase-like activity 1 (GGTLA1) gene, encodes complete protein.
AK312401 - Homo sapiens cDNA, FLJ92733.
BT009808 - Homo sapiens gamma-glutamyltransferase-like activity 1 mRNA, complete cds.
KJ891239 - Synthetic construct Homo sapiens clone ccsbBroadEn_00633 GGT5 gene, encodes complete protein.
DQ894214 - Synthetic construct Homo sapiens clone IMAGE:100008674; FLH168709.01L; RZPDo839C0593D gamma-glutamyltransferase-like activity 1 (GGTLA1) gene, encodes complete protein.
AK304522 - Homo sapiens cDNA FLJ52056 complete cds, moderately similar to Gamma-glutamyltransferase 5 precursor (EC 2.3.2.2).
CU687286 - Synthetic construct Homo sapiens gateway clone IMAGE:100023000 5' read GGTLA1 mRNA.
JD437693 - Sequence 418717 from Patent EP1572962.
JD151197 - Sequence 132221 from Patent EP1572962.
JD069258 - Sequence 50282 from Patent EP1572962.
JD104868 - Sequence 85892 from Patent EP1572962.
JD364789 - Sequence 345813 from Patent EP1572962.
JD150033 - Sequence 131057 from Patent EP1572962.
JD357746 - Sequence 338770 from Patent EP1572962.
JD527878 - Sequence 508902 from Patent EP1572962.
JD420283 - Sequence 401307 from Patent EP1572962.
JD260175 - Sequence 241199 from Patent EP1572962.
JD226838 - Sequence 207862 from Patent EP1572962.
JD366930 - Sequence 347954 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  BioCyc Knowledge Library
PWY-4041 - γ-glutamyl cycle
PWY-4061 - glutathione-mediated detoxification I
PWY-7112 - 4-hydroxy-2-nonenal detoxification
PWY66-375 - leukotriene biosynthesis

Reactome (by CSHL, EBI, and GO)

Protein P36269 (Reactome details) participates in the following event(s):

R-HSA-266046 GGT1, 5 dimers hydrolyse LTC4 to LTD4
R-HSA-5433072 GGTs hydrolyse glutamate from AFXBO-SG, AFNBO-SG
R-HSA-8943279 GGT dimers hydrolyse GSH
R-HSA-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX)
R-HSA-5423646 Aflatoxin activation and detoxification
R-HSA-174403 Glutathione synthesis and recycling
R-HSA-2142753 Arachidonic acid metabolism
R-HSA-211859 Biological oxidations
R-HSA-156590 Glutathione conjugation
R-HSA-8978868 Fatty acid metabolism
R-HSA-1430728 Metabolism
R-HSA-156580 Phase II - Conjugation of compounds
R-HSA-556833 Metabolism of lipids

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000327365.1, ENST00000327365.2, ENST00000327365.3, ENST00000327365.4, ENST00000327365.5, ENST00000327365.6, ENST00000327365.7, ENST00000327365.8, ENST00000327365.9, GGT5_HUMAN, GGTLA1, NM_004121, P36269, Q53XM9, Q6GMP0, Q96FC1, Q9UFM5, uc317sgp.1, uc317sgp.2
UCSC ID: ENST00000327365.10_7
RefSeq Accession: NM_004121.5
Protein: P36269 (aka GGT5_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.