Human Gene MARCHF8 (ENST00000319836.7_8) from GENCODE V47lift37
  Description: membrane associated ring-CH-type finger 8, transcript variant 2 (from RefSeq NM_145021.6)
Gencode Transcript: ENST00000319836.7_8
Gencode Gene: ENSG00000165406.16_13
Transcript (Including UTRs)
   Position: hg19 chr10:45,950,041-46,090,354 Size: 140,314 Total Exon Count: 7 Strand: -
Coding Region
   Position: hg19 chr10:45,953,687-46,028,659 Size: 74,973 Coding Exon Count: 6 

Page IndexSequence and LinksUniProtKB CommentsPrimersGene AllelesRNA-Seq Expression
RNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA DescriptionsOther Names
Model InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr10:45,950,041-46,090,354)mRNA (may differ from genome)Protein (291 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
OMIMPubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: MARH8_HUMAN
DESCRIPTION: RecName: Full=E3 ubiquitin-protein ligase MARCH8; EC=6.3.2.-; AltName: Full=Cellular modulator of immune recognition; Short=c-MIR; AltName: Full=Membrane-associated RING finger protein 8; AltName: Full=Membrane-associated RING-CH protein VIII; Short=MARCH-VIII; AltName: Full=RING finger protein 178;
FUNCTION: E3 ubiquitin-protein ligase that mediates ubiquitination of CD86 and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. May also promote ubiquitination and endocytosis of TFRC and FAS.
PATHWAY: Protein modification; protein ubiquitination.
SUBUNIT: Interacts with CD86.
SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Early endosome membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Broadly expressed. Present in immature dendritic cells (at protein level).
DOMAIN: The RING-CH-type zinc finger domain is required for E3 ligase activity.
SIMILARITY: Contains 1 RING-CH-type zinc finger.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 23.19 RPKM in Testis
Total median expression: 350.96 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -372.30750-0.496 Picture PostScript Text
3' UTR -1216.203646-0.334 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011016 - Znf_RING-CH
IPR013083 - Znf_RING/FYVE/PHD

Pfam Domains:
PF12906 - RING-variant domain

SCOP Domains:
57850 - RING/U-box

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2D8S - NMR MuPIT


ModBase Predicted Comparative 3D Structure on Q5T0T0
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004842 ubiquitin-protein transferase activity
GO:0008270 zinc ion binding
GO:0016740 transferase activity
GO:0046872 metal ion binding

Biological Process:
GO:0000209 protein polyubiquitination
GO:0002250 adaptive immune response
GO:0002376 immune system process
GO:0016567 protein ubiquitination

Cellular Component:
GO:0005764 lysosome
GO:0005765 lysosomal membrane
GO:0005768 endosome
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0030659 cytoplasmic vesicle membrane
GO:0031410 cytoplasmic vesicle
GO:0031901 early endosome membrane


-  Descriptions from all associated GenBank mRNAs
  BC066988 - Homo sapiens membrane-associated ring finger (C3HC4) 8, mRNA (cDNA clone MGC:87645 IMAGE:5267214), complete cds.
AK057289 - Homo sapiens cDNA FLJ32727 fis, clone TESTI2001008.
BC025394 - Homo sapiens membrane-associated ring finger (C3HC4) 8, mRNA (cDNA clone MGC:26766 IMAGE:4830278), complete cds.
AK313340 - Homo sapiens cDNA, FLJ93862, highly similar to Homo sapiens cellular modulator of immune recognition (c-MIR), mRNA.
KJ900468 - Synthetic construct Homo sapiens clone ccsbBroadEn_09862 MARCH8 gene, encodes complete protein.
AB529117 - Synthetic construct DNA, clone: pF1KB4828, Homo sapiens MARCH8 gene for membrane-associated ring finger (C3HC4) 8, without stop codon, in Flexi system.
AK307667 - Homo sapiens cDNA, FLJ97615.
AK025472 - Homo sapiens cDNA: FLJ21819 fis, clone HEP01185.
AL833316 - Homo sapiens mRNA; cDNA DKFZp313K127 (from clone DKFZp313K127).
AK125414 - Homo sapiens cDNA FLJ43425 fis, clone OCBBF2027197.
DQ578549 - Homo sapiens piRNA piR-46661, complete sequence.
DQ599376 - Homo sapiens piRNA piR-37442, complete sequence.
DQ597555 - Homo sapiens piRNA piR-35621, complete sequence.
DQ595937 - Homo sapiens piRNA piR-62049, complete sequence.
DL492557 - Novel nucleic acids.
DQ588226 - Homo sapiens piRNA piR-55338, complete sequence.
AK023636 - Homo sapiens cDNA FLJ13574 fis, clone PLACE1008625.
JD449536 - Sequence 430560 from Patent EP1572962.
JD392227 - Sequence 373251 from Patent EP1572962.
JD330116 - Sequence 311140 from Patent EP1572962.
JD422785 - Sequence 403809 from Patent EP1572962.
JD122963 - Sequence 103987 from Patent EP1572962.
JD484195 - Sequence 465219 from Patent EP1572962.
JD518273 - Sequence 499297 from Patent EP1572962.
JD267150 - Sequence 248174 from Patent EP1572962.
JD068150 - Sequence 49174 from Patent EP1572962.
JD214287 - Sequence 195311 from Patent EP1572962.
JD496395 - Sequence 477419 from Patent EP1572962.
JD544836 - Sequence 525860 from Patent EP1572962.
JD075211 - Sequence 56235 from Patent EP1572962.
JD106979 - Sequence 88003 from Patent EP1572962.
JD111395 - Sequence 92419 from Patent EP1572962.
JD137688 - Sequence 118712 from Patent EP1572962.
JD329636 - Sequence 310660 from Patent EP1572962.
JD498749 - Sequence 479773 from Patent EP1572962.
HZ461281 - WO 2015190586-A/94: Kit and method for detecting colon cancer.
HZ461675 - WO 2015190586-A/488: Kit and method for detecting colon cancer.
HZ461927 - WO 2015190591-A/82: Kit and method for detecting breast cancer.
HZ462482 - WO 2015190591-A/637: Kit and method for detecting breast cancer.
HZ463015 - WO 2015194535-A/109: Kit and method for detecting stomach cancer.
HZ463421 - WO 2015194535-A/515: Kit and method for detecting stomach cancer.
HZ464326 - WO 2015194615-A/144: Kit or device and method for detecting hepatic cancer.
HZ464780 - WO 2015194615-A/598: Kit or device and method for detecting hepatic cancer.
HZ465026 - WO 2015194627-A/79: Kit and method for detecting esophageal cancer.
HZ465441 - WO 2015194627-A/494: Kit and method for detecting esophageal cancer.
HZ480269 - JP 2015535430-A/775: TERMINALLY MODIFIED RNA.
HZ790200 - JP 2016504050-A/1945: SIGNAL-SENSOR POLYNUCLEOTIDES FOR THE ALTERATION OF CELLULAR PHENOTYPES.
JC512932 - Sequence 3185 from Patent WO2014113089.
LF161455 - JP 2016513950-A/1521: Oligomers with improved off-target profile.
LF632636 - WO 2016117582-A/65: miRNA biomarkers for mental disorders.
LP951765 - Sequence 271 from Patent WO2017157650.
LQ070520 - Sequence 844 from Patent EP2964234.
LY496209 - KR 1020170018406-A/79: ESOPHAGEAL CANCER DETECTION KIT OR DEVICE, AND DETECTION METHOD.
LY496624 - KR 1020170018406-A/494: ESOPHAGEAL CANCER DETECTION KIT OR DEVICE, AND DETECTION METHOD.
LY496924 - KR 1020170016485-A/94: COLORECTAL CANCER DETECTION KIT OR DEVICE, AND DETECTION METHOD.
LY497318 - KR 1020170016485-A/488: COLORECTAL CANCER DETECTION KIT OR DEVICE, AND DETECTION METHOD.
LY498227 - KR 1020170018411-A/144: LIVER CANCER DETECTION KIT OR DEVICE, AND DETECTION METHOD.
LY498681 - KR 1020170018411-A/598: LIVER CANCER DETECTION KIT OR DEVICE, AND DETECTION METHOD.
LY498930 - KR 1020170018412-A/82: BREAST CANCER DETECTION KIT OR DEVICE, AND METHOD FOR DETECTING BREAST CANCER.
LY499485 - KR 1020170018412-A/637: BREAST CANCER DETECTION KIT OR DEVICE, AND METHOD FOR DETECTING BREAST CANCER.
LY499828 - KR 1020170019425-A/109: STOMACH CANCER DETECTION KIT OR DEVICE, AND DETECTION METHOD.
LY500234 - KR 1020170019425-A/515: STOMACH CANCER DETECTION KIT OR DEVICE, AND DETECTION METHOD.
LZ238352 - WO 2017171048-A/13: Kit, device and method for detecting early pancreatic cancer or precancerous lesion of pancreas.
LZ238872 - WO 2017171048-A/533: Kit, device and method for detecting early pancreatic cancer or precancerous lesion of pancreas.
MS880245 - Sequence 82 from Patent EP3156498.
MS880800 - Sequence 637 from Patent EP3156498.
MS884111 - Sequence 94 from Patent EP3156499.
MS884505 - Sequence 488 from Patent EP3156499.
MS884947 - Sequence 109 from Patent EP3156503.
MS885353 - Sequence 515 from Patent EP3156503.
MS994217 - Sequence 79 from Patent EP3159406.
MS994632 - Sequence 494 from Patent EP3159406.
MA644021 - JP 2017113010-A/1945: SIGNAL-SENSOR POLYNUCLEOTIDES FOR THE ALTERATION OF CELLULAR PHENOTYPES.
MA731905 - JP 2017140048-A/775: TERMINALLY MODIFIED RNA.
MA405780 - WO 2018155427-A/554: A probe with reduced false positive binding, a method to desing the probe, and the use thereof.
MA801074 - JP 2018183181-A/1945: SIGNAL-SENSOR POLYNUCLEOTIDES FOR THE ALTERATION OF CELLULAR PHENOTYPES.
MA822222 - WO 2019004436-A/35: Kit, device, and method for detection of lung cancer.
MA822890 - WO 2019004436-A/703: Kit, device, and method for detection of lung cancer.
LY616116 - KR 1020180129785-A/13: KIT OR DEVICE FOR DETECTING EARLY STAGE PANCREATIC CANCER OR PANCREATIC CANCER PRECURSOR LESIONS AND DETECTION METHOD THEREFOR.
LY616636 - KR 1020180129785-A/533: KIT OR DEVICE FOR DETECTING EARLY STAGE PANCREATIC CANCER OR PANCREATIC CANCER PRECURSOR LESIONS AND DETECTION METHOD THEREFOR.
MB472652 - WO 2019208671-A/51: Kit or device and method for detecting bladder cancer.
MB473145 - WO 2019208671-A/544: Kit or device and method for detecting bladder cancer.

-  Other Names for This Gene
  Alternate Gene Symbols: B2R8E7, ENST00000319836.1, ENST00000319836.2, ENST00000319836.3, ENST00000319836.4, ENST00000319836.5, ENST00000319836.6, H0Y7C6, MARCH8, MARCHF8 , MARH8_HUMAN, MIR, NM_145021, Q5T0S8, Q5T0T0, Q8TC72, RNF178, uc317qtd.1, uc317qtd.2
UCSC ID: ENST00000319836.7_8
RefSeq Accession: NM_145021.6
Protein: Q5T0T0 (aka MARH8_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.