ID:RAB1B_HUMAN DESCRIPTION: RecName: Full=Ras-related protein Rab-1B; FUNCTION: Protein transport. Regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments. SUBUNIT: Interacts with MICAL1, MICAL2 and MICAL3. Interacts with GDI1; the interaction requires the GDP-bound state. Interacts with CHM/REP1; the interaction requires the GDP-bound form and is necessary for prenylation by GGTase II. SUBCELLULAR LOCATION: Membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm. Note=Targeted by REP1 to membranes of specific subcellular compartments including endoplasmic reticulum, Golgi apparatus, and intermediate vesicles between these two compartments. In the GDP-form, colocalizes with GDI in the cytoplasm (By similarity). PTM: Prenylated; by GGTase II, only after interaction of the substrate with Rab escort protein 1 (REP1). PTM: AMPylation at Tyr-77 by L.pneumophila DrrA occurs in the switch 2 region and leads to moderate inactivation of the GTPase activity. It appears to prolong the lifetime of the GTP state of RAB1B by restricting access of GTPase effectors to switch 2 and blocking effector-stimulated GTP hydrolysis, thereby rendering RAB1B constitutively active. It is later de-AMPylated by L.pneumophila SidD, releasing RAB1B from bacterial phagosomes. PTM: Phosphocholinated at Ser-76 by L.pneumophila AnkX, leading to displace GDP dissociation inhibitors (GDI). Both GDP-bound and GTP-bound forms can be phosphocholinated. Dephosphocholinated by L.pneumophila Lem3, restoring accessibility to L.pneumophila GTPase effector LepB. MISCELLANEOUS: Rab-1B binds GTP and GDP and possesses intrinsic GTPase activity (By similarity). SIMILARITY: Belongs to the small GTPase superfamily. Rab family.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
Pfam Domains: PF00025 - ADP-ribosylation factor family PF00071 - Ras family PF01926 - 50S ribosome-binding GTPase PF04670 - Gtr1/RagA G protein conserved region PF08477 - Ras of Complex, Roc, domain of DAPkinase PF09439 - Signal recognition particle receptor beta subunit
ModBase Predicted Comparative 3D Structure on Q9H0U4
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0006888 ER to Golgi vesicle-mediated transport GO:0006890 retrograde vesicle-mediated transport, Golgi to ER GO:0006914 autophagy GO:0015031 protein transport GO:0019068 virion assembly GO:0043687 post-translational protein modification GO:0048208 COPII vesicle coating GO:1903020 positive regulation of glycoprotein metabolic process GO:2000785 regulation of autophagosome assembly GO:0090557 establishment of endothelial intestinal barrier
AK292086 - Homo sapiens cDNA FLJ77824 complete cds, highly similar to Homo sapiens RAB1B, member RAS oncogene family (RAB1B), mRNA. AK315333 - Homo sapiens cDNA, FLJ96374, Homo sapiens RAB1B, member RAS oncogene family (RAB1B), mRNA. AL136635 - Homo sapiens mRNA; cDNA DKFZp564I172 (from clone DKFZp564I172). BC028331 - Homo sapiens RAB1B, member RAS oncogene family, mRNA (cDNA clone IMAGE:4537849). BC071169 - Homo sapiens RAB1B, member RAS oncogene family, mRNA (cDNA clone MGC:87903 IMAGE:6026121), complete cds. AF092437 - Homo sapiens rab1B mRNA, complete cds. AB464119 - Synthetic construct DNA, clone: pF1KB8198, Homo sapiens RAB1B gene for RAB1B, member RAS oncogene family, without stop codon, in Flexi system. AM393195 - Synthetic construct Homo sapiens clone IMAGE:100001881 for hypothetical protein (RAB1B gene). CU687104 - Synthetic construct Homo sapiens gateway clone IMAGE:100022950 5' read RAB1B mRNA. KJ894867 - Synthetic construct Homo sapiens clone ccsbBroadEn_04261 RAB1B gene, encodes complete protein. CR533462 - Homo sapiens full open reading frame cDNA clone RZPDo834B0317D for gene RAB1B, RAB1B, member RAS oncogene family; complete cds, incl. stopcodon. AJ245875 - Homo sapiens complete mRNA for putative small GTP-binding protein (rab1b). U66621 - Human small GTP-binding protein (rab1b) mRNA, partial cds. DQ584252 - Homo sapiens piRNA piR-51364, complete sequence. JD234356 - Sequence 215380 from Patent EP1572962. JD559134 - Sequence 540158 from Patent EP1572962. JD484373 - Sequence 465397 from Patent EP1572962. JD317964 - Sequence 298988 from Patent EP1572962. JD486338 - Sequence 467362 from Patent EP1572962. JD255870 - Sequence 236894 from Patent EP1572962. JD122622 - Sequence 103646 from Patent EP1572962. JD526025 - Sequence 507049 from Patent EP1572962. JD282774 - Sequence 263798 from Patent EP1572962. JD455342 - Sequence 436366 from Patent EP1572962. JD076092 - Sequence 57116 from Patent EP1572962. DQ586165 - Homo sapiens piRNA piR-53277, complete sequence. JD418623 - Sequence 399647 from Patent EP1572962. JD477099 - Sequence 458123 from Patent EP1572962. JD132450 - Sequence 113474 from Patent EP1572962. JD145266 - Sequence 126290 from Patent EP1572962. JD157581 - Sequence 138605 from Patent EP1572962. JD271118 - Sequence 252142 from Patent EP1572962. JD321569 - Sequence 302593 from Patent EP1572962. JD335150 - Sequence 316174 from Patent EP1572962. JD403790 - Sequence 384814 from Patent EP1572962. JD278311 - Sequence 259335 from Patent EP1572962. JD195951 - Sequence 176975 from Patent EP1572962. JD217238 - Sequence 198262 from Patent EP1572962. JD136971 - Sequence 117995 from Patent EP1572962. JD271750 - Sequence 252774 from Patent EP1572962. JD399673 - Sequence 380697 from Patent EP1572962. JD270882 - Sequence 251906 from Patent EP1572962. JD413628 - Sequence 394652 from Patent EP1572962. JD247444 - Sequence 228468 from Patent EP1572962. JD366933 - Sequence 347957 from Patent EP1572962. JD520795 - Sequence 501819 from Patent EP1572962. JD210831 - Sequence 191855 from Patent EP1572962. JD251429 - Sequence 232453 from Patent EP1572962. JD146457 - Sequence 127481 from Patent EP1572962. JD119780 - Sequence 100804 from Patent EP1572962. JD519472 - Sequence 500496 from Patent EP1572962. JD389330 - Sequence 370354 from Patent EP1572962. JD390755 - Sequence 371779 from Patent EP1572962.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q9H0U4 (Reactome details) participates in the following event(s):
R-NUL-2422970 Phosphorylation of Gorasp1, Golga2 and RAB1A by CDK1:CCNB R-HSA-6811415 RAB:GTP recruits GBF1 to the Golgi membrane R-HSA-8870466 RGGT:CHM binds RABs R-HSA-5694439 COPII coat binds TRAPPCII and RAB1:GDP R-HSA-6814833 TBC1D20 stimulates GTPase activity of RAB1, resulting in hydrolysis of GTP R-HSA-6807864 RAB1:GTP recruits GBF1 to the ERGIC R-HSA-6814831 TBC1D20 binds RAB1:GTP R-HSA-5694441 CSNK1D phosphorylates SEC23 R-HSA-8877475 TRAPPC complexes exchange GTP for GDP on RAB1 R-HSA-2172194 Recruitment of PLK1 to phosphorylated GORASP1 (GRASP65) R-HSA-2172183 Phosphorylation of GORASP1, GOLGA2 and RAB1A by CDK1:CCNB R-HSA-2214351 PLK1 phosphorylates GORASP1 R-HSA-6811411 GBF1 recruits ARF:GDP to the Golgi R-HSA-8870469 RGGT geranylgeranylates RAB proteins R-HSA-5694409 Nucleotide exchange on RAB1 R-HSA-5694418 RAB1:GTP binds USO1 and GORASP1:GOLGA2 R-HSA-6807866 GBF1 recruits ARF:GDP to the ERGIC R-HSA-6811426 Retrograde COPI vesicles bind kinesin and microtubules R-HSA-6811418 ARFGAPs stimulate ARF GTPase activity at the Golgi membrane R-HSA-6811423 Retrograde vesicle is tethered at the ER by the NRZ complex and t-SNAREs R-HSA-6811414 GBF1 stimulates nucleotide exchange on ARF R-HSA-6811412 Active ARF recruits coatomer to the Golgi R-HSA-6807868 GBF1 stimulates ARF nucleotide exchange R-HSA-6807872 Active ARF recruits coatomer R-HSA-6811417 ARFGAP, cargo, vSNARES and p24 proteins bind COPI vesicles at Golgi R-HSA-6807875 ARFGAP, cargo, v-SNAREs and p24 proteins bind nascent COPI complex R-HSA-6809003 ERGIC-to-Golgi vesicles bind dynein:dynactin R-HSA-6807877 ARFGAPs stimulate ARF GTPase activity R-HSA-6809011 cis-Golgi t-SNAREs bind YKT6 on tethered vesicle R-HSA-6809006 Vesicle is tethered through binding GOLGA2:GORASP1, GOLGB1 and the COG complex R-HSA-6811434 COPI-dependent Golgi-to-ER retrograde traffic R-HSA-8873719 RAB geranylgeranylation R-HSA-204005 COPII-mediated vesicle transport R-HSA-6807878 COPI-mediated anterograde transport R-HSA-8876198 RAB GEFs exchange GTP for GDP on RABs R-HSA-162658 Golgi Cisternae Pericentriolar Stack Reorganization R-HSA-8856688 Golgi-to-ER retrograde transport R-HSA-597592 Post-translational protein modification R-HSA-199977 ER to Golgi Anterograde Transport R-HSA-9007101 Rab regulation of trafficking R-HSA-68875 Mitotic Prophase R-HSA-6811442 Intra-Golgi and retrograde Golgi-to-ER traffic R-HSA-392499 Metabolism of proteins R-HSA-199991 Membrane Trafficking R-HSA-948021 Transport to the Golgi and subsequent modification R-HSA-68886 M Phase R-HSA-5653656 Vesicle-mediated transport R-HSA-446203 Asparagine N-linked glycosylation R-HSA-69278 Cell Cycle (Mitotic) R-HSA-1640170 Cell Cycle
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Common Gene Haplotype Alleles 
