Human Gene THNSL2 (ENST00000674334.2_4) from GENCODE V47lift37
  Description: threonine synthase like 2, transcript variant 1 (from RefSeq NM_018271.5)
Gencode Transcript: ENST00000674334.2_4
Gencode Gene: ENSG00000144115.18_13
Transcript (Including UTRs)
   Position: hg19 chr2:88,469,865-88,486,146 Size: 16,282 Total Exon Count: 9 Strand: +
Coding Region
   Position: hg19 chr2:88,472,670-88,485,642 Size: 12,973 Coding Exon Count: 8 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr2:88,469,865-88,486,146)mRNA (may differ from genome)Protein (484 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: THNS2_HUMAN
DESCRIPTION: RecName: Full=Threonine synthase-like 2; Short=TSH2; EC=4.2.3.-; AltName: Full=Secreted osteoclastogenic factor of activated T-cells; Short=SOFAT;
FUNCTION: Isoform 1: Acts as a catabolic phospho-lyase on both gamma- and beta-phosphorylated substrates. Degrades O-phospho- threonine (PThr) to alpha-ketobutyrate, ammonia and phosphate (By similarity).
FUNCTION: Isoform SOLAT: Potent inducer of osteoblastic production of IL6. May act to exacerbate inflammation and/or bone turnover under inflammatory conditions.
COFACTOR: Pyridoxal phosphate (By similarity).
SUBCELLULAR LOCATION: Isoform SOLAT: Secreted. Note=Secreted by activated T-cells via a calcineurin-independent pathway.
SIMILARITY: Belongs to the threonine synthase family.
SEQUENCE CAUTION: Sequence=AAX88906.1; Type=Erroneous gene model prediction; Sequence=BAA91904.1; Type=Erroneous initiation; Note=Translation N-terminally extended;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
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+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -57.20123-0.465 Picture PostScript Text
3' UTR -199.70504-0.396 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR004450 - Thr_synthase_like
IPR001926 - Trp_syn_b_sub_like_PLP_eny_SF

Pfam Domains:
PF00291 - Pyridoxal-phosphate dependent enzyme
PF14821 - Threonine synthase N terminus

SCOP Domains:
53686 - Tryptophan synthase beta subunit-like PLP-dependent enzymes

ModBase Predicted Comparative 3D Structure on Q86YJ6
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003674 molecular_function
GO:0005125 cytokine activity
GO:0016829 lyase activity
GO:0030170 pyridoxal phosphate binding
GO:0070905 serine binding
GO:0004795 threonine synthase activity

Biological Process:
GO:0008150 biological_process
GO:0009071 serine family amino acid catabolic process
GO:0010469 regulation of receptor activity
GO:0016311 dephosphorylation
GO:0046360 2-oxobutyrate biosynthetic process
GO:0009088 threonine biosynthetic process

Cellular Component:
GO:0005575 cellular_component
GO:0005576 extracellular region
GO:0005615 extracellular space


-  Descriptions from all associated GenBank mRNAs
  AK315831 - Homo sapiens cDNA, FLJ79480 complete cds.
AK289486 - Homo sapiens cDNA FLJ76990 complete cds.
BC064423 - Homo sapiens threonine synthase-like 2 (S. cerevisiae), mRNA (cDNA clone MGC:70718 IMAGE:5752279), complete cds.
AK095303 - Homo sapiens cDNA FLJ37984 fis, clone CTONG2010652, weakly similar to Threonine synthase (EC 4.2.3.1).
BC050699 - Homo sapiens hypothetical protein FLJ10916, mRNA (cDNA clone IMAGE:6064461), partial cds.
BC035315 - Homo sapiens threonine synthase-like 2 (S. cerevisiae), mRNA (cDNA clone IMAGE:4750266), partial cds.
AK001778 - Homo sapiens cDNA FLJ10916 fis, clone OVARC1000309, weakly similar to THREONINE SYNTHASE (EC 4.2.99.2).
JD489943 - Sequence 470967 from Patent EP1572962.
JD527628 - Sequence 508652 from Patent EP1572962.
JD072684 - Sequence 53708 from Patent EP1572962.
JD062295 - Sequence 43319 from Patent EP1572962.
BC047758 - Homo sapiens threonine synthase-like 2 (S. cerevisiae), mRNA (cDNA clone IMAGE:5761156), partial cds.
JD469862 - Sequence 450886 from Patent EP1572962.
AL832746 - Homo sapiens mRNA; cDNA DKFZp686B0921 (from clone DKFZp686B0921).
HM185274 - Homo sapiens secreted osteoclastogenic factor of activated T cells mRNA, complete cds, alternatively spliced.
AK092823 - Homo sapiens cDNA FLJ35504 fis, clone SMINT2009259.
DQ594935 - Homo sapiens piRNA piR-61047, complete sequence.
JD246098 - Sequence 227122 from Patent EP1572962.
DQ583459 - Homo sapiens piRNA piR-50571, complete sequence.
JD113038 - Sequence 94062 from Patent EP1572962.
JD336940 - Sequence 317964 from Patent EP1572962.
JD206010 - Sequence 187034 from Patent EP1572962.
JD464636 - Sequence 445660 from Patent EP1572962.
JD555104 - Sequence 536128 from Patent EP1572962.
JD210711 - Sequence 191735 from Patent EP1572962.
JD563523 - Sequence 544547 from Patent EP1572962.
JD386927 - Sequence 367951 from Patent EP1572962.
JD432762 - Sequence 413786 from Patent EP1572962.
JD276212 - Sequence 257236 from Patent EP1572962.
JD121968 - Sequence 102992 from Patent EP1572962.
JD065988 - Sequence 47012 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: B3KTB1, B5MDX8, B7WPF8, D9ZZB8, ENST00000674334.1, NM_018271, Q6P2M7, Q6PI27, Q86YJ6, Q9NV54, THNS2_HUMAN, uc329ydo.1, uc329ydo.2
UCSC ID: ENST00000674334.2_4
RefSeq Accession: NM_018271.5
Protein: Q86YJ6 (aka THNS2_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.