Human Gene TPST2 (ENST00000338754.9_8) from GENCODE V47lift37

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Description: tyrosylprotein sulfotransferase 2, transcript variant 2 (from RefSeq NM_003595.5)
Gencode Transcript: ENST00000338754.9_8
Gencode Gene: ENSG00000128294.16_11
Transcript (Including UTRs)
Position: hg19 chr22:26,917,962-26,986,096 Size: 68,135 Total Exon Count: 7 Strand: -
Coding Region
Position: hg19 chr22:26,924,187-26,937,596 Size: 13,410 Coding Exon Count: 4

Page Index	Sequence and Links	UniProtKB Comments	Primers	CTD	Gene Alleles
RNA-Seq Expression	Microarray Expression	RNA Structure	Protein Structure	Other Species	GO Annotations
mRNA Descriptions	Pathways	Other Names	Model Information	Methods

Data last updated at UCSC: 2024-08-22 23:36:26

Sequence and Links to Tools and Databases

Genomic Sequence (chr22:26,917,962-26,986,096)			mRNA (may differ from genome)		Protein (377 aa)
Gene Sorter	Genome Browser	Other Species FASTA	VisiGene	Gene interactions	Table Schema
AlphaFold	BioGPS	Ensembl	Entrez Gene	ExonPrimer	GeneCards
HGNC	MGI	OMIM	PubMed	Reactome	UniProtKB
BioGrid CRISPR DB

Comments and Description Text from UniProtKB


	ID: TPST2_HUMAN DESCRIPTION: RecName: Full=Protein-tyrosine sulfotransferase 2; EC=2.8.2.20; AltName: Full=Tyrosylprotein sulfotransferase 2; Short=TPST-2; FUNCTION: Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides. CATALYTIC ACTIVITY: 3'-phosphoadenylyl sulfate + protein tyrosine = adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate. SUBCELLULAR LOCATION: Golgi apparatus membrane; Single-pass type II membrane protein (By similarity). TISSUE SPECIFICITY: Widely expressed. SIMILARITY: Belongs to the protein sulfotransferase family.

Primer design for this transcript

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3

Comparative Toxicogenomics Database (CTD)


	The following chemicals interact with this gene C049325 1,2-dithiol-3-thione C111118 2',3,3',4',5-pentachloro-4-hydroxybiphenyl C548651 2-(1'H-indolo-3'-carbonyl)thiazole-4-carboxylic acid methyl ester D000082 Acetaminophen D000535 Aluminum D004052 Diethylnitrosamine D004997 Ethinyl Estradiol D005472 Fluorouracil D008070 Lipopolysaccharides C568608 PCI 5002 more ... click here to view the complete list

Common Gene Haplotype Alleles


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RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)


	Highest median expression: 94.32 RPKM in Pancreas Total median expression: 494.96 RPKM View in GTEx track of Genome Browser View at GTEx portal View GTEx Body Map

Microarray Expression Data


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mRNA Secondary Structure of 3' and 5' UTRs

Region	Fold Energy	Bases	Energy/Base	Display As
5' UTR	-114.60	240	-0.477	Picture	PostScript	Text
3' UTR	-1483.67	4279	-0.347	Picture	PostScript	Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

Protein Domain and Structure Information

InterPro Domains: Graphical view of domain structure
IPR026634 - TPST

Pfam Domains:
PF00685 - Sulfotransferase domain
PF13469 - Sulfotransferase family

SCOP Domains:
52540 - P-loop containing nucleoside triphosphate hydrolases

Protein Data Bank (PDB) 3-D Structure
MuPIT help

3AP1 - X-ray MuPIT

3AP2 - X-ray MuPIT

3AP3 - X-ray MuPIT

ModBase Predicted Comparative 3D Structure on O60704


Front	Top	Side

The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

Orthologous Genes in Other Species

Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.

Mouse	Rat	Zebrafish	D. melanogaster	C. elegans	S. cerevisiae
No ortholog	No ortholog	No ortholog	No ortholog	No ortholog	No ortholog
Gene Details	Gene Details
Gene Sorter	Gene Sorter
	RGD

Gene Ontology (GO) Annotations with Structured Vocabulary


	Molecular Function: GO:0008476 protein-tyrosine sulfotransferase activity GO:0016740 transferase activity GO:0042803 protein homodimerization activity Biological Process: GO:0006478 peptidyl-tyrosine sulfation GO:0050427 3'-phosphoadenosine 5'-phosphosulfate metabolic process Cellular Component: GO:0000139 Golgi membrane GO:0005783 endoplasmic reticulum GO:0005794 Golgi apparatus GO:0016020 membrane GO:0016021 integral component of membrane

Descriptions from all associated GenBank mRNAs


	AK093872 - Homo sapiens cDNA FLJ36553 fis, clone TRACH2008478. DQ600639 - Homo sapiens piRNA piR-38705, complete sequence. JD249607 - Sequence 230631 from Patent EP1572962. JD378066 - Sequence 359090 from Patent EP1572962. JD447164 - Sequence 428188 from Patent EP1572962. JD401710 - Sequence 382734 from Patent EP1572962. JD160255 - Sequence 141279 from Patent EP1572962. JD452946 - Sequence 433970 from Patent EP1572962. JD074914 - Sequence 55938 from Patent EP1572962. AK074538 - Homo sapiens cDNA FLJ90057 fis, clone HEMBA1003101, highly similar to Protein-tyrosine sulfotransferase 2 (EC 2.8.2.20). AJ006198 - Homo sapiens mRNA for tyrosyl sulfotransferase-2. BC017509 - Homo sapiens tyrosylprotein sulfotransferase 2, mRNA (cDNA clone MGC:23597 IMAGE:4857366), complete cds. AK057171 - Homo sapiens cDNA FLJ32609 fis, clone STOMA2000032. AF049891 - Homo sapiens tyrosylprotein sulfotransferase-2 mRNA, complete cds. BC001057 - Homo sapiens tyrosylprotein sulfotransferase 2, mRNA (cDNA clone MGC:1931 IMAGE:3509627), complete cds. AL136623 - Homo sapiens mRNA; cDNA DKFZp564B212 (from clone DKFZp564B212). AK075139 - Homo sapiens cDNA FLJ90658 fis, clone PLACE1004775, highly similar to Protein-tyrosine sulfotransferase 2 (EC 2.8.2.20). AK304545 - Homo sapiens cDNA FLJ54247 complete cds, highly similar to Protein-tyrosine sulfotransferase 2 (EC 2.8.2.20). JD292732 - Sequence 273756 from Patent EP1572962. JD384385 - Sequence 365409 from Patent EP1572962. JD259603 - Sequence 240627 from Patent EP1572962. JD142840 - Sequence 123864 from Patent EP1572962. CR533528 - Homo sapiens full open reading frame cDNA clone RZPDo834H0119D for gene TPST2, tyrosylprotein sulfotransferase 2; complete cds, incl. stopcodon. AF061254 - Homo sapiens tyrosylprotein sulfotransferase-2 mRNA, complete cds. CR456597 - Homo sapiens TPST2 full length open reading frame (ORF) cDNA clone (cDNA clone C22ORF:pGEM.TPST2.V3). JD415449 - Sequence 396473 from Patent EP1572962. CU013197 - Homo sapiens TPST2, mRNA (cDNA clone IMAGE:100000550), complete cds, with stop codon, in Gateway system. KJ892537 - Synthetic construct Homo sapiens clone ccsbBroadEn_01931 TPST2 gene, encodes complete protein. KJ905963 - Synthetic construct Homo sapiens clone ccsbBroadEn_15633 TPST2 gene, encodes complete protein. DQ893600 - Synthetic construct clone IMAGE:100006230; FLH180690.01X; RZPDo839E12134D tyrosylprotein sulfotransferase 2 (TPST2) gene, encodes complete protein. AB528266 - Synthetic construct DNA, clone: pF1KE0545, Homo sapiens TPST2 gene for tyrosylprotein sulfotransferase 2, without stop codon, in Flexi system. CU013485 - Homo sapiens TPST2, mRNA (cDNA clone IMAGE:100000454), complete cds, without stop codon, in Gateway system. CU676002 - Synthetic construct Homo sapiens gateway clone IMAGE:100017134 5' read TPST2 mRNA. CU674534 - Synthetic construct Homo sapiens gateway clone IMAGE:100017133 5' read TPST2 mRNA. JD476476 - Sequence 457500 from Patent EP1572962. JD210811 - Sequence 191835 from Patent EP1572962. JD386830 - Sequence 367854 from Patent EP1572962. JD503735 - Sequence 484759 from Patent EP1572962. JD450713 - Sequence 431737 from Patent EP1572962. JD362177 - Sequence 343201 from Patent EP1572962. JD215660 - Sequence 196684 from Patent EP1572962.

Biochemical and Signaling Pathways


	Reactome (by CSHL, EBI, and GO) Protein O60704 (Reactome details) participates in the following event(s): R-HSA-8954262 TPST1,2 transfer SO4(2-) from PAPS to PODXL2 R-HSA-156584 Cytosolic sulfonation of small molecules R-HSA-156580 Phase II - Conjugation of compounds R-HSA-211859 Biological oxidations R-HSA-1430728 Metabolism

Other Names for This Gene


	Alternate Gene Symbols: B3KQA7, ENST00000338754.1, ENST00000338754.2, ENST00000338754.3, ENST00000338754.4, ENST00000338754.5, ENST00000338754.6, ENST00000338754.7, ENST00000338754.8, NM_003595, O60704, Q6FI98, Q9H0V4, TPST2_HUMAN, uc317vcz.1, uc317vcz.2 UCSC ID: ENST00000338754.9_8 RefSeq Accession: NM_003595.5 Protein: O60704 (aka TPST2_HUMAN)

Gene Model Information


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Methods, Credits, and Use Restrictions


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