ID:WDR61_HUMAN DESCRIPTION: RecName: Full=WD repeat-containing protein 61; AltName: Full=Meiotic recombination REC14 protein homolog; AltName: Full=SKI8 homolog; Short=Ski8; FUNCTION: Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of MLL1; it promotes leukemogenesis though association with MLL-rearranged oncoproteins, such as MLL-MLLT3/AF9 and MLL-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Required for mono- and trimethylation on histone H3 'Lys-4' (H3K4me3), dimethylation on histone H3 'Lys-79' (H3K4me3). Required for Hox gene transcription. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1C. SUBUNIT: Component of the PAF1 complex, which consists of CDC73, PAF1, LEO1, CTR9, RTF1 and WDR61. Component of the SKI complex which consists of WDR61, SKIV2L and TTC37. INTERACTION: Q6PD62:CTR9; NbExp=9; IntAct=EBI-358545, EBI-1019583; SUBCELLULAR LOCATION: Nucleus. Cytoplasm (Probable). SIMILARITY: Contains 7 WD repeats.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q9GZS3
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
LF208497 - JP 2014500723-A/16000: Polycomb-Associated Non-Coding RNAs. AK025098 - Homo sapiens cDNA FLJ21445 fis, A-COL04457. BC010080 - Homo sapiens WD repeat domain 61, mRNA (cDNA clone MGC:19469 IMAGE:4329599), complete cds. AK024754 - Homo sapiens cDNA: FLJ21101 fis, clone CAS04682. AF100786 - Homo sapiens G protein beta subunit-like protein mRNA, complete cds. AF127799 - Homo sapiens G protein beta subunit-like protein mRNA, complete cds. AF309553 - Homo sapiens meiotic recombination protein REC14 mRNA, complete cds. CR457333 - Homo sapiens full open reading frame cDNA clone RZPDo834H0413D for gene REC14, recombination protein REC14; complete cds, incl. stopcodon. HQ447187 - Synthetic construct Homo sapiens clone IMAGE:100070481; CCSB006964_02 WD repeat domain 61 (WDR61) gene, encodes complete protein. KJ899642 - Synthetic construct Homo sapiens clone ccsbBroadEn_09036 WDR61 gene, encodes complete protein. CU679601 - Synthetic construct Homo sapiens gateway clone IMAGE:100018096 5' read WDR61 mRNA. LF377090 - JP 2014500723-A/184593: Polycomb-Associated Non-Coding RNAs. LF377091 - JP 2014500723-A/184594: Polycomb-Associated Non-Coding RNAs. AK300838 - Homo sapiens cDNA FLJ50697 complete cds. AK304617 - Homo sapiens cDNA FLJ61675 complete cds, highly similar to WD repeat protein 61. AK311320 - Homo sapiens cDNA, FLJ18362. AK310477 - Homo sapiens cDNA, FLJ17519. AL109709 - Homo sapiens mRNA full length insert cDNA clone EUROIMAGE 43432. LF377099 - JP 2014500723-A/184602: Polycomb-Associated Non-Coding RNAs. JD495990 - Sequence 477014 from Patent EP1572962. JD229559 - Sequence 210583 from Patent EP1572962. JD443264 - Sequence 424288 from Patent EP1572962. MA444074 - JP 2018138019-A/16000: Polycomb-Associated Non-Coding RNAs. MA612667 - JP 2018138019-A/184593: Polycomb-Associated Non-Coding RNAs. MA612668 - JP 2018138019-A/184594: Polycomb-Associated Non-Coding RNAs. MA612676 - JP 2018138019-A/184602: Polycomb-Associated Non-Coding RNAs.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q9GZS3 (Reactome details) participates in the following event(s):
R-HSA-112379 Recruitment of elongation factors to form elongation complex R-HSA-8942099 RNF20:RNF40 binds PAF complex, Ubiquitin:UBE2A,B (Ubiquitin:RAD6), WAC and Histone H2B R-HSA-113429 Elongating transcript encounters a lesion in the template R-HSA-112385 Addition of nucleotides leads to transcript elongation R-HSA-113411 2-4 nt.backtracking of Pol II complex on the template leading to elongation pausing R-HSA-113412 Pol II elongation complex moves on the template as transcript elongates R-HSA-113414 7-14 nt. Backtracking of Pol II complex on the template leading to elongation arrest R-HSA-112392 Resumption of elongation after recovery from pausing R-HSA-113413 TFIIS-mediated recovery of elongation from arrest R-HSA-112395 Abortive termination of elongation after arrest R-HSA-112396 Separation of elongating transcript from template R-HSA-430028 Exosome Complex hydrolyzes mRNA by 3' to 5' exoribonuclease digestion R-HSA-112382 Formation of RNA Pol II elongation complex R-HSA-8866654 E3 ubiquitin ligases ubiquitinate target proteins R-HSA-674695 RNA Polymerase II Pre-transcription Events R-HSA-75955 RNA Polymerase II Transcription Elongation R-HSA-8852135 Protein ubiquitination R-HSA-73857 RNA Polymerase II Transcription R-HSA-597592 Post-translational protein modification R-HSA-74160 Gene expression (Transcription) R-HSA-429958 mRNA decay by 3' to 5' exoribonuclease R-HSA-392499 Metabolism of proteins R-HSA-429914 Deadenylation-dependent mRNA decay R-HSA-8953854 Metabolism of RNA
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
