Human Gene WIF1 (ENST00000286574.9_7) from GENCODE V48lift37
  Description: WNT inhibitory factor 1 (from RefSeq NM_007191.5)
Gencode Transcript: ENST00000286574.9_7
Gencode Gene: ENSG00000156076.10_10
Transcript (Including UTRs)
   Position: hg19 chr12:65,444,406-65,515,085 Size: 70,680 Total Exon Count: 10 Strand: -
Coding Region
   Position: hg19 chr12:65,445,129-65,514,971 Size: 69,843 Coding Exon Count: 10 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2025-04-10 15:10:12

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr12:65,444,406-65,515,085)mRNA (may differ from genome)Protein (379 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIneXtProtOMIMPubMed
ReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: WIF1_HUMAN
DESCRIPTION: RecName: Full=Wnt inhibitory factor 1; Short=WIF-1; Flags: Precursor;
FUNCTION: Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.
SUBCELLULAR LOCATION: Secreted.
SIMILARITY: Contains 5 EGF-like domains.
SIMILARITY: Contains 1 WIF domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: WIF1
Diseases sorted by gene-association score: salivary gland adenoid cystic carcinoma (6), esophageal basaloid squamous cell carcinoma (5), colorectal cancer (1)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 53.32 RPKM in Brain - Caudate (basal ganglia)
Total median expression: 284.19 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -42.10114-0.369 Picture PostScript Text
3' UTR -145.90723-0.202 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000742 - EG-like_dom
IPR013032 - EGF-like_CS
IPR003306 - WIF
IPR013309 - Wnt-inh

Pfam Domains:
PF02019 - WIF domain
PF12661 - Human growth factor-like EGF

SCOP Domains:
57196 - EGF/Laminin
57184 - Growth factor receptor domain

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2D3J - NMR MuPIT 2YGN - X-ray MuPIT 2YGO - X-ray MuPIT 2YGP - X-ray MuPIT 2YGQ - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q9Y5W5
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details Gene Details  
Gene SorterGene Sorter Gene Sorter  
 RGDEnsembl   
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding
GO:0017147 Wnt-protein binding

Biological Process:
GO:0007165 signal transduction
GO:0007275 multicellular organism development
GO:0016055 Wnt signaling pathway
GO:0030178 negative regulation of Wnt signaling pathway
GO:0045600 positive regulation of fat cell differentiation

Cellular Component:
GO:0005576 extracellular region


-  Descriptions from all associated GenBank mRNAs
  EF058195 - Homo sapiens HMGA2/WIF1 fusion protein mRNA, partial cds.
BX647427 - Homo sapiens mRNA; cDNA DKFZp686J0699 (from clone DKFZp686J0699).
AF122922 - Homo sapiens Wnt inhibitory factor-1 mRNA, complete cds.
AY358344 - Homo sapiens clone DNA33094 WIF-1 (UNQ191) mRNA, complete cds.
BC018037 - Homo sapiens WNT inhibitory factor 1, mRNA (cDNA clone MGC:26240 IMAGE:4801033), complete cds.
JD019172 - Sequence 196 from Patent EP1572962.
JD168120 - Sequence 149144 from Patent EP1572962.
JD032994 - Sequence 14018 from Patent EP1572962.
AK301818 - Homo sapiens cDNA FLJ54458 complete cds, highly similar to Wnt inhibitory factor 1 precursor.
JD235482 - Sequence 216506 from Patent EP1572962.
JD258121 - Sequence 239145 from Patent EP1572962.
JD286189 - Sequence 267213 from Patent EP1572962.
JD546137 - Sequence 527161 from Patent EP1572962.
KJ898375 - Synthetic construct Homo sapiens clone ccsbBroadEn_07769 WIF1 gene, encodes complete protein.
DQ892394 - Synthetic construct clone IMAGE:100005024; FLH185931.01X; RZPDo839G10148D WNT inhibitory factor 1 (WIF1) gene, encodes complete protein.
DQ895609 - Synthetic construct Homo sapiens clone IMAGE:100010069; FLH185927.01L; RZPDo839G10147D WNT inhibitory factor 1 (WIF1) gene, encodes complete protein.
EU263623 - Homo sapiens HMGA2ex3/WIF1ex10 fusion protein (HMGA2/WIF1 fusion) mRNA, partial cds.
EU263622 - Homo sapiens HMGA2ex3/WIF1ex9 fusion protein (HMGA2/WIF1 fusion) mRNA, partial cds.
EU263621 - Homo sapiens HMGA2ex3/WIF1ex4 fusion protein (HMGA2/WIF1 fusion) mRNA, partial cds.
EU263620 - Homo sapiens HMGA2ex5/WIF1ex3 fusion protein (HMGA2/WIF1 fusion) mRNA, partial cds.
EU263619 - Homo sapiens HMGA2ex3/WIF1ex3 fusion protein (HMGA2/WIF1 fusion) mRNA, partial cds.
JD307541 - Sequence 288565 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  BioCarta from NCI Cancer Genome Anatomy Project
h_wntPathway - WNT Signaling Pathway

Reactome (by CSHL, EBI, and GO)

Protein Q9Y5W5 (Reactome details) participates in the following event(s):

R-HSA-3769370 WIF1 binds WNTs
R-HSA-3772470 Negative regulation of TCF-dependent signaling by WNT ligand antagonists
R-HSA-201681 TCF dependent signaling in response to WNT
R-HSA-195721 Signaling by WNT
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000286574.1, ENST00000286574.2, ENST00000286574.3, ENST00000286574.4, ENST00000286574.5, ENST00000286574.6, ENST00000286574.7, ENST00000286574.8, NM_007191, Q6UXI1, Q8WVG4, Q9Y5W5, uc317kkl.1, uc317kkl.2, UNQ191/PRO217, WIF1_HUMAN
UCSC ID: ENST00000286574.9_7
RefSeq Accession: NM_007191.5
Protein: Q9Y5W5 (aka WIF1_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.