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UCSC Genome Browser Gene Interaction Graph
Gene interactions and pathways from curated databases and text-mining
Invest Ophthalmol Vis Sci 1999, PMID: 10359315

Pax-6 interactions with TATA-box-binding protein and retinoblastoma protein.

Cvekl, A; Kashanchi, F; Brady, J N; Piatigorsky, J

OBJECTIVE

To identify proteins that physically interact with Pax-6, a paired domain- and homeodomain (HD)-containing transcription factor that is a key regulator of eye development.

METHODS

Protein-protein interactions involving Pax-6, TATA-box-binding protein (TPB), and retinoblastoma protein were studied using affinity chromatography with Pax-6 as ligand, glutathione-S-transferase (GST) pull-down assays, and immunoprecipitations.

RESULTS

The authors have shown that Pax-6 is a sequence-specific activator of many crystallin genes, all containing a TATA box, in the lens. Others have shown that lens fiber cell differentiation, characterized by temporally and spatially regulated crystallin gene expression, depends on retinoblastoma protein. In the present study it was shown that Pax-6 interacted with the TBP, the DNA-binding subunit of general transcription complex TFIID. GST pull-down assays indicated that this interaction was mediated by the Pax-6 HD, with a substantial role for its N-terminal arm and first two alpha-helices. The experiments also indicated a binding role for the C-terminal-activation domain of the protein. In addition, the present study showed that the HD of Pax-6 interacted with retinoblastoma protein. Immunoprecipitation experiments confirmed retinoblastoma protein/Pax-6 complexes in lens nuclear extracts.

CONCLUSIONS

Blending the present results with those in the literature suggests that Pax-6 and retinoblastoma protein participate in overlapping regulatory pathways controlling epithelial cell division, fiber cell elongation, and crystallin gene expression during lens development.

Document information provided by NCBI PubMed

Text Mining Data

Dashed line = No text mining data

Manually curated Databases

  • IRef Biogrid Interaction: TAF1 — PAX6 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PAX6 — RB1 (direct interaction, pull down)
  • IRef Biogrid Interaction: PAX3 — TBP (direct interaction, pull down)
  • IRef Biogrid Interaction: TBP — TP53 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TAF1 — TP53 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TBP — PAX6 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TBP — PAX6 (direct interaction, pull down)
  • IRef Hprd Interaction: PAX6 — TBP (in vivo)
  • IRef Hprd Interaction: PAX6 — TBP (in vitro)
In total, 6 gene pairs are associated to this article in curated databases