Gene interactions and pathways from curated databases and text-mining
J Cell Biol 2001, PMID: 11149922

A functional interaction between the survival motor neuron complex and RNA polymerase II.

Pellizzoni, L; Charroux, B; Rappsilber, J; Mann, M; Dreyfuss, G

The survival motor neuron (SMN) protein, the protein product of the spinal muscular atrophy (SMA) disease gene, plays a role in the assembly and regeneration of small nuclear ribonucleoproteins (snRNPs) and spliceosomes. By nanoelectrospray mass spectrometry, we identified RNA helicase A (RHA) as an SMN complex-associated protein. RHA is a DEAH box RNA helicase which binds RNA polymerase II (pol II) and reportedly functions in transcription. SMN interacts with RHA in vitro, and this interaction is impaired in mutant SMNs found in SMA patients. Coimmunoprecipitation demonstrated that the SMN complex is associated with pol II, snRNPs, and RHA in vivo. In vitro experiments suggest that RHA mediates the association of SMN with the COOH-terminal domain of pol II. Moreover, transfection of cells with a dominant negative mutant of SMN, SMNDeltaN27, causes accumulation of pol II, snRNPs, and RHA in nuclear structures that contain the known markers of gems and coiled bodies, and inhibits RNA pol I and pol II transcription in vivo. These findings indicate a functional as well as physical association of the SMN complex with pol II and suggest a role for the SMN complex in the assembly of the pol II transcription/processing machinery.

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Text Mining Data

SMN → RHA: " In vitro experiments suggest that RHA mediates the association of SMN with the COOH-terminal domain of pol II "

Manually curated Databases

  • IRef Biogrid Interaction: DHX9 — GEMIN2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: GEMIN4 — DHX9 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DHX9 — GTF2B (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DDX20 — DHX9 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DHX9 — TBP (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DHX9 — SMN1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DHX9 — SMN1 (direct interaction, pull down)
  • MIPS CORUM SMN-PolII-RHA complex: SMN-PolII-RHA complex complex (DDX20-DHX9-GEMIN4-GTF2B-POLR2A-GEMIN2-SNRPB-SNRPB2-SNRPN-TBP)
  • IRef Corum Interaction: Complex of 11 proteins (association, coimmunoprecipitation)
  • IRef Hprd Interaction: GEMIN2 — DHX9 (in vivo)
  • IRef Hprd Interaction: DHX9 — POLR2A (in vivo)
  • IRef Hprd Interaction: GEMIN4 — DHX9 (in vitro)
  • IRef Hprd Interaction: GEMIN4 — DHX9 (in vivo)
  • IRef Hprd Interaction: Complex of 65 proteins (in vivo)
  • IRef Hprd Interaction: DHX9 — GTF2B (in vitro)
  • IRef Hprd Interaction: DHX9 — GTF2B (in vivo)
  • IRef Hprd Interaction: SMN1 — POLR2A (in vivo)
  • IRef Hprd Interaction: SMN1 — POLR2A (in vitro)
  • IRef Hprd Interaction: DDX20 — DHX9 (in vitro)
  • IRef Hprd Interaction: DDX20 — DHX9 (in vivo)
  • IRef Hprd Interaction: SNRPB2 — DHX9 (in vivo)
  • IRef Hprd Interaction: SNRPB — DHX9 (in vivo)
  • IRef Hprd Interaction: DHX9 — TBP (in vivo)
  • IRef Hprd Interaction: SMN1 — DHX9 (in vitro)
  • IRef Hprd Interaction: SMN1 — DHX9 (in vivo)
  • IRef Ophid Interaction: DHX9 — GEMIN2 (aggregation, confirmational text mining)
  • IRef Ophid Interaction: POLR2A — DHX9 (aggregation, confirmational text mining)
  • IRef Ophid Interaction: GEMIN4 — DHX9 (aggregation, confirmational text mining)
  • IRef Ophid Interaction: DHX9 — GTF2B (aggregation, confirmational text mining)
  • IRef Ophid Interaction: SNRPB2 — DHX9 (aggregation, confirmational text mining)
  • IRef Ophid Interaction: DHX9 — SNRPB (aggregation, confirmational text mining)
  • IRef Ophid Interaction: DHX9 — TBP (aggregation, confirmational text mining)
In total, 60 gene pairs are associated to this article in curated databases