Gene interactions and pathways from curated databases and text-mining
Dev Cell 2003, PMID: 12791267

Prophase destruction of Emi1 by the SCF(betaTrCP/Slimb) ubiquitin ligase activates the anaphase promoting complex to allow progression beyond prometaphase.

Margottin-Goguet, Florence; Hsu, Jerry Y; Loktev, Alexander; Hsieh, Harn Mei; Reimann, Julie D R; Jackson, Peter K

Progression through mitosis occurs because cyclin B/Cdc2 activation induces the anaphase promoting complex (APC) to cause cyclin B destruction and mitotic exit. To ensure that cyclin B/Cdc2 does not prematurely activate the APC in early mitosis, there must be a mechanism delaying APC activation. Emi1 is a protein capable of inhibiting the APC in S and G2. We show here that Emi1 is phosphorylated by Cdc2, and on a DSGxxS consensus site, is subsequently recognized by the SCF(betaTrCP/Slimb) ubiquitin ligase and destroyed, thus providing a delay for APC activation. Failure of betaTrCP-dependent Emi1 destruction stabilizes APC substrates and results in mitotic catastrophe including centrosome overduplication, potentially explaining mitotic deficiencies in Drosophila Slimb/betaTrCP mutants. We hypothesize that Emi1 destruction relieves a late prophase checkpoint for APC activation.

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Text Mining Data

anaphase promoting complex (APC) → B/Cdc2: " Progression through mitosis occurs because cyclin B/Cdc2 activation induces the anaphase promoting complex (APC) to cause cyclin B destruction and mitotic exit "

anaphase promoting complex (APC) → cyclin: " Progression through mitosis occurs because cyclin B/Cdc2 activation induces the anaphase promoting complex (APC) to cause cyclin B destruction and mitotic exit "

APC → B/Cdc2: " To ensure that cyclin B/Cdc2 does not prematurely activate the APC in early mitosis, there must be a mechanism delaying APC activation "

Manually curated Databases

  • Reactome Reaction: CUL1 → FZR1 (reaction)
  • Reactome Reaction: FBXO5 → CDK1 (reaction)
  • Reactome Reaction: FBXO5 → BTRC (indirect_complex)
  • Reactome Reaction: FZR1 → SKP1 (indirect_complex)
  • Reactome Reaction: FBXO5 → CUL1 (indirect_complex)
  • Reactome Reaction: CDC20 → SKP1 (indirect_complex)
  • Reactome Reaction: FBXO5 → BTRC (reaction)
  • Reactome Reaction: CDK1 → FZR1 (reaction)
  • Reactome Reaction: FZR1 → SKP1 (reaction)
  • Reactome Reaction: FBXO5 → SKP1 (reaction)
  • Reactome Reaction: FBXO5 → CCNB1 (reaction)
  • Reactome Reaction: CUL1 → CDC20 (indirect_complex)
  • Reactome Reaction: CDC20 → SKP1 (reaction)
  • Reactome Reaction: FBXO5 → CDC20 (direct_complex)
  • Reactome Reaction: CCNB1 → FZR1 (reaction)
  • Reactome Reaction: FBXO5 → CUL1 (reaction)
  • Reactome Reaction: CDC20 → CDK1 (reaction)
  • Reactome Reaction: CUL1 → CDC20 (reaction)
  • Reactome Reaction: FBXO5 → FZR1 (direct_complex)
  • Reactome Reaction: BTRC → FZR1 (reaction)
  • Reactome Reaction: CUL1 → FZR1 (indirect_complex)
  • Reactome Reaction: CDC20 → CCNB1 (reaction)
  • Reactome Reaction: BTRC → CDC20 (reaction)
  • Reactome Reaction: FBXO5 → SKP1 (indirect_complex)
  • Reactome Reaction: BTRC → FZR1 (indirect_complex)
  • Reactome Reaction: BTRC → CDC20 (indirect_complex)
In total, 17 gene pairs are associated to this article in curated databases