Gene interactions and pathways from curated databases and text-mining
Cell Signal 2007, PMID: 16978839

Neuregulin-1 only induces trans-phosphorylation between ErbB receptor heterodimer partners.

Li, Zhaomin; Mei, Yanai; Liu, Xifu; Zhou, Mingdong

ErbB2, ErbB3 and ErbB4 are members of the Epidermal Growth Factor Receptor (EGFR) sub-family of Receptor Tyrosine Kinases (RTKs). Neuregulin-1 (NRG-1) is a ligand of ErbB3 and ErbB4 receptors. NRG-1-induced ErbB2/ErbB3 or ErbB2/ErbB4 heterodimerization, followed by receptor phosphorylation, plays multiple biological roles. To precisely determine the phosphorylation status of each ErbB receptor in ErbB2/ErbB3 and ErbB2/ErbB4 heterodimers, an immunoprecipitation-recapture of the ErbB receptors was performed to exclude any co-immunoprecipitated heterodimer partners from cells with co-expression of ErbB2/ErbB3, ErbB2/ErbB4, or ErbB2/ErbB4D843N, a kinase-inactive ErbB4 mutant, in which the aspartic acid at 843 (D843) was replaced by an asparagine (N). Here, we provide direct biochemical evidence that ErbB2 was only trans-phosphorylated by ErbB4, but not by ErbB3 or ErbB4D843N. By contrast, ErbB3, ErbB4 and ErbB4D843N were trans-phosphorylated by ErbB2 in the co-transfected cells. Therefore, we conclude that trans-phosphorylation, but not cis-phosphorylation occurred between ErbB2/ErbB3 and ErbB2/ErbB4 heterodimer partners by NRG-1 stimulation.

Document information provided by NCBI PubMed

Text Mining Data

ErbB2/ErbB3 → NRG-1: " NRG-1 induced ErbB2/ErbB3 or ErbB2/ErbB4 heterodimerization, followed by receptor phosphorylation, plays multiple biological roles "

ErbB2/ErbB3 → NRG-1: " NRG-1 induced ErbB2/ErbB3 or ErbB2/ErbB4 heterodimerization, followed by receptor phosphorylation, plays multiple biological roles "

ErbB2/ErbB4 → NRG-1: " NRG-1 induced ErbB2/ErbB3 or ErbB2/ErbB4 heterodimerization, followed by receptor phosphorylation, plays multiple biological roles "

Manually curated Databases

  • Reactome Reaction: EREG → HBEGF (reaction)
  • Reactome Reaction: NRG1 → NRG1 (reaction)
  • Reactome Reaction: EGF → ERBB3 (reaction)
  • Reactome Reaction: EGFR → NRG4 (reaction)
  • Reactome Reaction: EGF → NRG4 (reaction)
  • Reactome Reaction: BTC → EGF (reaction)
  • Reactome Reaction: EREG → ERBB2IP (reaction)
  • Reactome Reaction: ERBB2 → NRG4 (reaction)
  • Reactome Reaction: BTC → EGFR (reaction)
  • Reactome Reaction: BTC → NRG2 (reaction)
  • Reactome Reaction: ERBB3 → EREG (reaction)
  • Reactome Reaction: EREG → NRG1 (reaction)
  • Reactome Reaction: ERBB3 → ERBB2IP (reaction)
  • Reactome Reaction: ERBB2 → NRG1 (reaction)
  • Reactome Reaction: ERBB4 → NRG1 (reaction)
  • Reactome Reaction: ERBB3 → HBEGF (reaction)
  • Reactome Reaction: ERBB2 → ERBB4 (reaction)
  • Reactome Reaction: HBEGF → NRG1 (reaction)
  • Reactome Reaction: BTC → HBEGF (reaction)
  • Reactome Reaction: HBEGF → CDC37 (reaction)
  • Reactome Reaction: EGFR → ERBB3 (reaction)
  • Reactome Reaction: HSP90AA1 → ERBB2IP (reaction)
  • Reactome Reaction: EGFR → ERBB2 (reaction)
  • Reactome Reaction: NRG2 → CDC37 (reaction)
  • Reactome Reaction: BTC → BTC (reaction)
  • Reactome Reaction: EREG → NRG2 (reaction)
  • Reactome Reaction: ERBB2 → ERBB2 (reaction)
  • Reactome Reaction: ERBB3 → CDC37 (reaction)
  • Reactome Reaction: ERBB2 → HSP90AA1 (reaction)
  • Reactome Reaction: EREG → ERBB4 (reaction)
  • Reactome Reaction: EGFR → HBEGF (reaction)
  • Reactome Reaction: EGFR → HSP90AA1 (reaction)
  • Reactome Reaction: EGF → HSP90AA1 (reaction)
  • Reactome Reaction: NRG4 → ERBB4 (reaction)
  • Reactome Reaction: HBEGF → NRG2 (reaction)
  • Reactome Reaction: NRG1 → CDC37 (reaction)
  • Reactome Reaction: BTC → EREG (reaction)
  • Reactome Reaction: ERBB4 → ERBB4 (reaction)
  • Reactome Reaction: BTC → CDC37 (reaction)
  • Reactome Reaction: HBEGF → ERBB4 (reaction)
  • Reactome Reaction: EREG → EREG (reaction)
  • Reactome Reaction: NRG2 → ERBB4 (reaction)
  • Reactome Reaction: EGF → HBEGF (reaction)
  • Reactome Reaction: HSP90AA1 → NRG1 (reaction)
  • Reactome Reaction: EGF → NRG2 (reaction)
  • Reactome Reaction: BTC → ERBB2IP (reaction)
  • Reactome Reaction: NRG4 → CDC37 (reaction)
  • Reactome Reaction: EGFR → NRG1 (reaction)
  • Reactome Reaction: ERBB2 → ERBB2IP (reaction)
  • Reactome Reaction: EGFR → EGF (reaction)
  • Reactome Reaction: EGF → ERBB2IP (reaction)
  • Reactome Reaction: ERBB2 → NRG2 (reaction)
  • Reactome Reaction: ERBB2 → EREG (reaction)
  • Reactome Reaction: HSP90AA1 → NRG2 (reaction)
  • Reactome Reaction: BTC → NRG1 (reaction)
  • Reactome Reaction: HBEGF → HSP90AA1 (reaction)
  • Reactome Reaction: NRG2 → NRG4 (reaction)
  • Reactome Reaction: HSP90AA1 → NRG4 (reaction)
  • Reactome Reaction: ERBB2IP → CDC37 (reaction)
  • Reactome Reaction: NRG4 → ERBB2IP (reaction)
  • Reactome Reaction: HBEGF → ERBB2IP (reaction)
  • Reactome Reaction: EREG → NRG4 (reaction)
  • Reactome Reaction: ERBB3 → NRG4 (reaction)
  • Reactome Reaction: BTC → ERBB4 (reaction)
  • Reactome Reaction: NRG4 → NRG4 (reaction)
  • Reactome Reaction: ERBB3 → ERBB3 (reaction)
  • Reactome Reaction: HBEGF → HBEGF (reaction)
  • Reactome Reaction: BTC → ERBB3 (reaction)
  • Reactome Reaction: NRG1 → ERBB4 (reaction)
  • Reactome Reaction: ERBB3 → HSP90AA1 (reaction)
  • Reactome Reaction: ERBB3 → ERBB4 (reaction)
  • Reactome Reaction: ERBB2 → HBEGF (reaction)
  • Reactome Reaction: EGFR → ERBB4 (reaction)
  • Reactome Reaction: BTC → ERBB2 (reaction)
  • Reactome Reaction: EGF → NRG1 (reaction)
  • Reactome Reaction: NRG2 → ERBB2IP (reaction)
  • Reactome Reaction: EGFR → NRG2 (reaction)
  • Reactome Reaction: EGF → CDC37 (reaction)
  • Reactome Reaction: EREG → HSP90AA1 (reaction)
  • Reactome Reaction: NRG2 → NRG1 (reaction)
  • Reactome Reaction: HSP90AA1 → CDC37 (reaction)
  • Reactome Reaction: ERBB3 → NRG2 (reaction)
  • Reactome Reaction: EGF → ERBB4 (reaction)
  • Reactome Reaction: EREG → CDC37 (reaction)
  • Reactome Reaction: BTC → HSP90AA1 (reaction)
  • Reactome Reaction: BTC → NRG4 (reaction)
  • Reactome Reaction: EGF → EREG (reaction)
  • Reactome Reaction: NRG2 → NRG2 (reaction)
  • Reactome Reaction: EGFR → EREG (reaction)
  • Reactome Reaction: NRG4 → NRG1 (reaction)
  • Reactome Reaction: ERBB3 → NRG1 (reaction)
  • Reactome Reaction: EGF → EGF (reaction)
  • Reactome Reaction: HSP90AA1 → ERBB4 (reaction)
  • Reactome Reaction: ERBB2IP → ERBB4 (reaction)
  • Reactome Reaction: ERBB2 → CDC37 (reaction)
  • Reactome Reaction: EGFR → EGFR (reaction)
  • Reactome Reaction: ERBB4 → ERBB2IP (reaction)
  • Reactome Reaction: ERBB2IP → NRG1 (reaction)
  • Reactome Reaction: HBEGF → NRG4 (reaction)
  • Reactome Reaction: EGFR → CDC37 (reaction)
  • Reactome Reaction: ERBB4 → CDC37 (reaction)
  • Reactome Reaction: EGFR → ERBB2IP (reaction)
  • Reactome Reaction: ERBB2 → ERBB3 (reaction)
  • Reactome Reaction: EGF → ERBB2 (reaction)
In total, 102 gene pairs are associated to this article in curated databases