EMBO Rep 2008,
PMID: 18451880
Okada, Maiko; Takezawa, Shin-ichiro; Mezaki, Yoshihiro; Yamaoka, Ikuko; Takada, Ichiro; Kitagawa, Hirochika; Kato, Shigeaki
The female sex steroid hormone oestrogen stimulates both cell proliferation and cell differentiation in target tissues. These biological actions are mediated primarily through nuclear oestrogen receptors (ERs). The ligand-dependent transactivation of ERs requires several nuclear co-regulator complexes; however, the cell-cycle-dependent associations of these complexes are poorly understood. By using a synchronization system, we found that the transactivation function of ERalpha at G2/M was lowered. Biochemical approaches showed that ERalpha associated with two discrete classes of ATP-dependent chromatin-remodelling complex in a cell-cycle-dependent manner. The components of the NuRD-type complex were identified as G2/M-phase-specific ERalpha co-repressors. Thus, our results indicate that the transactivation function of ERalpha is cell-cycle dependent and is coupled with a cell-cycle-dependent association of chromatin-remodelling complexes.
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Text Mining Data
Dashed line = No text mining data
Manually curated Databases
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IRef Biogrid Interaction:
ESR1
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SMARCA4
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MTA2
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ESR1
(direct interaction, pull down)
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IRef Biogrid Interaction:
MTA2
—
ESR1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
ESR1
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SMARCB1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
ESR1
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SMARCC2
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
HDAC1
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ESR1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
ESR1
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MTA1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
ESR1
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MTA1
(direct interaction, pull down)
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IRef Biogrid Interaction:
ESR1
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CHD3
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
SRC
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ESR1
(direct interaction, pull down)
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IRef Biogrid Interaction:
MTA3
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ESR1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MTA3
—
ESR1
(direct interaction, pull down)
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IRef Intact Interaction:
Complex of ESR1-SMARCC2-SMARCB1-SMARCA4
(association, anti tag coimmunoprecipitation)
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IRef Intact Interaction:
ESR1
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SMARCA4
(physical association, anti bait coimmunoprecipitation)
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IRef Intact Interaction:
ESR1
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SMARCA4
(colocalization, fluorescence microscopy)
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IRef Intact Interaction:
Complex of CHD3-MTA1-HDAC1-ESR1
(association, anti bait coimmunoprecipitation)
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IRef Intact Interaction:
MTA2
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ESR1
(physical association, pull down)
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IRef Intact Interaction:
NCOA1
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ESR1
(physical association, pull down)
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IRef Intact Interaction:
ESR1
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MTA1
(physical association, pull down)
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IRef Intact Interaction:
ESR1
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CHD3
(colocalization, fluorescence microscopy)
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IRef Intact Interaction:
ESR1
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MTA3
(physical association, pull down)
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IRef Intact Interaction:
Complex of 13 proteins
(association, anti tag coimmunoprecipitation)
In total, 23 gene pairs are associated to this article in curated databases