We have a suspicion that you are an automated web bot software, not a real user. To keep our site fast for other users, we have slowed down this page. The slowdown will gradually disappear. If you think this is a mistake, please contact us at genome-www@soe.ucsc.edu. Also note that all data for hgGeneGraph can be obtained through our public MySQL server and all our software source code is available and can be installed locally onto your own computer. If you are unsure how to use these resources, do not hesitate to contact us.
UCSC Genome Browser Gene Interaction Graph
Gene interactions and pathways from curated databases and text-mining
J Cell Biochem 2008, PMID: 18729074

Identification of novel Smad2 and Smad3 associated proteins in response to TGF-beta1.

Brown, Kimberly A; Ham, Amy-Joan L; Clark, Cara N; Meller, Nahum; Law, Brian K; Chytil, Anna; Cheng, Nikki; Pietenpol, Jennifer A; Moses, Harold L

Transforming growth factor-beta 1 (TGF-beta1) is an important growth inhibitor of epithelial cells and insensitivity to this cytokine results in uncontrolled cell proliferation and can contribute to tumorigenesis. TGF-beta1 signals through the TGF-beta type I and type II receptors, and activates the Smad pathway via phosphorylation of Smad2 and Smad3. Since little is known about the selective activation of Smad2 versus Smad3, we set out to identify novel Smad2 and Smad3 interacting proteins in epithelial cells. A non-transformed human cell line was transduced with Myc-His(6)-Smad2 or Myc-His(6)-Smad3-expressing retrovirus and was treated with TGF-beta1. Myc-His(6)-Smad2 or Myc-His(6)-Smad3 was purified by tandem affinity purification, eluates were subject to SDS-PAGE and Colloidal Blue staining, and select protein bands were digested with trypsin. The resulting tryptic peptides were analyzed by liquid chromatography (LC) and tandem mass spectrometry (MS/MS) and the SEQUEST algorithm was employed to identify proteins in the bands. A number of proteins that are known to interact with Smad2 or Smad3 were detected in the eluates. In addition, a number of putative novel Smad2 and Smad3 associated proteins were identified that have functions in cell proliferation, apoptosis, actin cytoskeleton regulation, cell motility, transcription, and Ras or insulin signaling. Specifically, the interaction between Smad2/3 and the Cdc42 guanine nucleotide exchange factor, Zizimin1, was validated by co-immunoprecipitation. The discovery of these novel Smad2 and/or Smad3 associated proteins may reveal how Smad2 and Smad3 are regulated and/or uncover new functions of Smad2 and Smad3 in TGF-beta1 signaling.

Document information provided by NCBI PubMed

Text Mining Data

Dashed line = No text mining data

Manually curated Databases

  • IRef Biogrid Interaction: FAM83G — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DNAJA2 — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PCID2 — SMAD2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SNW1 — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: U2AF2 — SMAD2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SLC25A12 — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SIK3 — SMAD2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: YY1 — SMAD2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD3 — RPL3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TCP1 — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD2 — PSPC1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD2 — DOCK9 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: YTHDF2 — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD2 — DCAF7 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD1 — SMAD2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: BUB3 — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD2 — KLF5 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DDX3Y — SMAD2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD3 — PSPC1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HSPA4 — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CDK11A — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DDX3X — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DDX3Y — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DDX4 — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD5 — SMAD2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD5 — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HSPA4 — SMAD2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD3 — ZFYVE9 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD2 — ITGB4 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PTPN6 — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD1 — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD2 — C1orf116 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: LEMD3 — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DNAJA2 — SMAD2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD2 — SMAD4 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD3 — CSNK1D (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMURF2 — SMAD2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD2 — RIN1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD3 — EPB41L5 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: MAPK1 — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RAB11FIP5 — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD3 — DCAF7 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD2 — BUB3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SLC25A3 — SMAD2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD3 — ERBB2IP (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DGKA — SMAD2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD2 — CAD (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: IRS1 — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD3 — C1orf116 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD3 — ITGB4 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PABPC1 — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: U2AF2 — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SQRDL — SMAD2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SLC25A5 — SMAD2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DHX8 — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD2 — EPB41L5 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD3 — LRCH1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD2 — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SIK3 — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD2 — CPSF7 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD2 — CCT6A (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: WWP2 — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SKI — SMAD2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: WRNIP1 — SMAD2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SNW1 — SMAD2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD2 — CSNK1D (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMURF2 — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RPL4 — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD4 — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD2 — SKIL (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD2 — ERBB2IP (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: USP7 — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: YY1 — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DHX8 — SMAD2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DOCK9 — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD2 — ZFYVE9 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD3 — VAV2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD3 — DYRK1A (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD3 — SLC9A3R2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD2 — WWP2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: LEMD3 — SMAD2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CHD8 — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD3 — OPA1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD3 — ATXN2L (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: ATAD3B — SMAD2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SQRDL — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SLC25A6 — SMAD2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PJA1 — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: FAM83G — SMAD2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMAD2 — OPA1 (physical association, affinity chromatography technology)
  • IRef Intact Interaction: Complex of SMAD4-DOCK9-SMAD3 (association, anti tag coimmunoprecipitation)
  • IRef Intact Interaction: DOCK9 — SMAD2 (physical association, tandem affinity purification)
  • IRef Intact Interaction: DOCK9 — SMAD2 (physical association, anti tag coimmunoprecipitation)
  • IRef Intact Interaction: Complex of 48 proteins (physical association, tandem affinity purification)
  • IRef Intact Interaction: Complex of 43 proteins (physical association, tandem affinity purification)
  • IRef Intact Interaction: SMAD2 — SMAD4 (physical association, tandem affinity purification)
  • IRef Intact Interaction: SMAD3 — SMAD4 (physical association, tandem affinity purification)
  • IRef Intact Interaction: DOCK9 — SMAD3 (physical association, anti tag coimmunoprecipitation)
  • IRef Intact Interaction: DOCK9 — SMAD3 (physical association, tandem affinity purification)
  • IRef Intact Interaction: Complex of SMAD2-DOCK9-SMAD4 (association, anti tag coimmunoprecipitation)
In total, 1541 gene pairs are associated to this article in curated databases