Gene interactions and pathways from curated databases and text-mining
Cell 2009, PMID: 19490896

Assembly pathway of the Mammalian proteasome base subcomplex is mediated by multiple specific chaperones.

Kaneko, Takeumi; Hamazaki, Jun; Iemura, Shun-Ichiro; Sasaki, Katsuhiro; Furuyama, Kaori; Natsume, Tohru; Tanaka, Keiji; Murata, Shigeo

The 26S proteasome is an enzymatic complex that degrades ubiquitinated proteins in eukaryotic cells. It is composed of the 20S core particle (CP) and the 19S regulatory particle (RP). The latter is further divided into the lid and base subcomplexes. While the mechanism involved in the assembly of the CP is well investigated, that of the RP is poorly understood. Here, we show that the formation of the mammalian base subcomplex involves three distinct modules, where specific pairs of ATPase subunits are associated with the distinct chaperones p28, S5b, or p27. The process of base formation starts from association of the p28-Rpt3-Rpt6-Rpn14 complex with the S5b-Rpt1-Rpt2-Rpn1 complex, followed by incorporation of the p27-Rpt5-Rpt4 complex and Rpn2, where p28, S5b, and p27 regulate the associations between the modules. These chaperones dissociate before completion of 26S proteasome formation. Our results demonstrate that base assembly is facilitated by multiple proteasome-dedicated chaperones, like CP assembly.

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Text Mining Data

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Manually curated Databases

  • IRef Biogrid Interaction: PSMD5 — PSMD2 (colocalization, biochemical)
  • IRef Biogrid Interaction: PSMC2 — PSMC1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PSMD5 — PSMC2 (colocalization, biochemical)
  • IRef Biogrid Interaction: PSMD5 — PSMC2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PSMC3 — PSMD9 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PSMC3 — PSMD9 (colocalization, biochemical)
  • IRef Biogrid Interaction: PSMC4 — PSMD10 (colocalization, biochemical)
  • IRef Biogrid Interaction: PSMC4 — PSMD10 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PAAF1 — PSMC5 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PSMC6 — PSMD9 (colocalization, biochemical)
  • IRef Biogrid Interaction: PSMC3 — PSMC6 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PSMC4 — PSMC5 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PSMD10 — PSMC5 (colocalization, biochemical)
  • IRef Biogrid Interaction: PSMD5 — PSMC1 (colocalization, biochemical)
  • IRef Intact Interaction: Complex of PSMD1-PSMC5-PSMC1-PSMA1-PSMD2 (physical association, cosedimentation in solution)
  • IRef Intact Interaction: Complex of PSMC3-PSMD9-PSMC6-PSMD9-PSMC6-PSMC3-PSMC3-PSMD9-PSMC6 (association, anti tag coimmunoprecipitation)
  • IRef Intact Interaction: Complex of PSMC3-PSMC4-PSMC5-PSMD10-PSMC6 (association, anti tag coimmunoprecipitation)
  • IRef Intact Interaction: Complex of 25 proteins (association, anti bait coimmunoprecipitation)
  • IRef Intact Interaction: Complex of PSMD10-PSMD3-PSMD2-PSMC2-PSMD1-PSMC5-PSMC4-PSMC1 (association, anti tag coimmunoprecipitation)
  • IRef Intact Interaction: Complex of 11 proteins (physical association, cosedimentation in solution)
  • IRef Intact Interaction: PSMC2 — PSMC1 (direct interaction, anti tag coimmunoprecipitation)
  • IRef Intact Interaction: PSMC4 — PSMC1 (physical association, cosedimentation in solution)
  • IRef Intact Interaction: PSMC6 — PSMC1 (physical association, cosedimentation in solution)
  • IRef Intact Interaction: Complex of PSMD2-PSMC2-PSMD1-PSMC4-PAAF1-PSMD10-PSMC5-PSMC1 (association, anti tag coimmunoprecipitation)
  • IRef Intact Interaction: PSMD5 — PSMC2 (direct interaction, anti tag coimmunoprecipitation)
  • IRef Intact Interaction: PSMC6 — PSMC4 (physical association, cosedimentation in solution)
  • IRef Intact Interaction: PSMC3 — PSMD9 (direct interaction, anti tag coimmunoprecipitation)
  • IRef Intact Interaction: Complex of PSMD10-PSMD10-PAAF1-PSMC5-PSMC4-PSMC5-PSMC4-PAAF1 (association, anti tag coimmunoprecipitation)
  • IRef Intact Interaction: PSMD10 — PSMC4 (direct interaction, anti tag coimmunoprecipitation)
  • IRef Intact Interaction: PAAF1 — PSMC5 (direct interaction, anti tag coimmunoprecipitation)
  • IRef Intact Interaction: Complex of 11 proteins (association, anti bait coimmunoprecipitation)
  • IRef Intact Interaction: Complex of 33 proteins (association, anti tag coimmunoprecipitation)
  • IRef Intact Interaction: Complex of PSMC3-PSMC6-PSMC1-PSMD2-PSMC2 (association, anti tag coimmunoprecipitation)
  • IRef Intact Interaction: PSMC3 — PSMC6 (direct interaction, anti tag coimmunoprecipitation)
  • IRef Intact Interaction: Complex of 14 proteins (association, anti tag coimmunoprecipitation)
  • IRef Intact Interaction: Complex of 20 proteins (association, anti tag coimmunoprecipitation)
  • IRef Intact Interaction: Complex of 11 proteins (association, anti tag coimmunoprecipitation)
  • IRef Intact Interaction: PSMC1 — PSMD2 (direct interaction, anti tag coimmunoprecipitation)
  • IRef Intact Interaction: PSMC4 — PSMC5 (direct interaction, anti tag coimmunoprecipitation)
  • IRef Intact Interaction: Complex of PSMC4-PSMC5-PSMD1-PSMC1-PSMC2-PSMD2 (association, anti bait coimmunoprecipitation)
In total, 565 gene pairs are associated to this article in curated databases