Gene interactions and pathways from curated databases and text-mining
Cell 2010, PMID: 20603019

TIF1gamma controls erythroid cell fate by regulating transcription elongation.

Bai, Xiaoying; Kim, Jonghwan; Yang, Zhongan; Jurynec, Michael J; Akie, Thomas E; Lee, Joseph; LeBlanc, Jocelyn; Sessa, Anna; Jiang, Hong; DiBiase, Anthony; Zhou, Yi; Grunwald, David J; Lin, Shuo; Cantor, Alan B; Orkin, Stuart H; Zon, Leonard I

Recent genome-wide studies have demonstrated that pausing of RNA polymerase II (Pol II) occurred on many vertebrate genes. By genetic studies in the zebrafish tif1gamma mutant moonshine we found that loss of function of Pol II-associated factors PAF or DSIF rescued erythroid gene transcription in tif1gamma-deficient animals. Biochemical analysis established physical interactions among TIF1gamma, the blood-specific SCL transcription complex, and the positive elongation factors p-TEFb and FACT. Chromatin immunoprecipitation assays in human CD34(+) cells supported a TIF1gamma-dependent recruitment of positive elongation factors to erythroid genes to promote transcription elongation by counteracting Pol II pausing. Our study establishes a mechanism for regulating tissue cell fate and differentiation through transcription elongation.

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Text Mining Data

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Manually curated Databases

  • IRef Biogrid Interaction: SSBP2 — TRIM33 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CDK9 — TAL1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CDK9 — SUPT16H (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SSBP3 — TRIM33 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TRIM33 — TRIM33 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CDK9 — TRIM33 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SUPT16H — TAL1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SSBP4 — TRIM33 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TRIM33 — RUNX1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: LYL1 — TRIM33 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TRIM33 — TCF4 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TCF3 — TRIM33 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: LDB1 — TRIM33 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SUPT16H — TRIM33 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TAL1 — TRIM33 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CBFA2T3 — TRIM33 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SSRP1 — TRIM33 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TCF12 — TRIM33 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SUPT16H — SUPT16H (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CDK9 — CDK9 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TAL1 — TAL1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RUNX1T1 — TRIM33 (physical association, affinity chromatography technology)
  • IRef Intact Interaction: Complex of 38 proteins (association, anti tag coimmunoprecipitation)
  • IRef Intact Interaction: Complex of 21 proteins (association, molecular sieving)
In total, 674 gene pairs are associated to this article in curated databases