J Biol Chem 2011,
PMID: 21778237
Mao, Xicheng; Gluck, Nathan; Chen, Baozhi; Starokadomskyy, Petro; Li, Haiying; Maine, Gabriel N; Burstein, Ezra
Cullin RING ligases (CRLs), the most prolific class of ubiquitin ligase enzymes, are multimeric complexes that regulate a wide range of cellular processes. CRL activity is regulated by CAND1 (Cullin-associated Nedd8-dissociated protein 1), an inhibitor that promotes the dissociation of substrate receptor components from the CRL. We demonstrate here that COMMD1 (copper metabolism MURR1 domain-containing 1), a factor previously found to promote ubiquitination of various substrates, regulates CRL activation by antagonizing CAND1 binding. We show that COMMD1 interacts with multiple Cullins, that the COMMD1-Cul2 complex cannot bind CAND1, and that, conversely, COMMD1 can actively displace CAND1 from CRLs. These findings highlight a novel mechanism of CRL activation and suggest that CRL regulation may underlie the pleiotropic activities of COMMD1.
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Text Mining Data
COMMD1 — CAND1: "
COMMD1 ( copper metabolism MURR1 domain containing protein 1 )
regulates Cullin RING ligases by preventing
CAND1 ( Cullin associated Nedd8 dissociated protein 1 ) binding
"
Manually curated Databases
-
IRef Biogrid Interaction:
CUL1
—
COMMD1
(physical association, affinity chromatography technology)
-
IRef Biogrid Interaction:
CUL1
—
COMMD1
(direct interaction, pull down)
-
IRef Biogrid Interaction:
CAND1
—
CUL1
(direct interaction, pull down)
-
IRef Biogrid Interaction:
COMMD1
—
CUL3
(physical association, affinity chromatography technology)
-
IRef Biogrid Interaction:
COMMD1
—
CUL3
(direct interaction, pull down)
-
IRef Biogrid Interaction:
CUL7
—
COMMD1
(direct interaction, pull down)
-
IRef Biogrid Interaction:
CAND1
—
CUL2
(direct interaction, pull down)
-
IRef Biogrid Interaction:
CUL1
—
SKP1
(direct interaction, pull down)
-
IRef Biogrid Interaction:
CUL2
—
RBX1
(direct interaction, pull down)
-
IRef Biogrid Interaction:
COMMD1
—
CUL4A
(direct interaction, pull down)
-
IRef Biogrid Interaction:
COMMD1
—
CUL2
(physical association, affinity chromatography technology)
-
IRef Biogrid Interaction:
COMMD1
—
CUL2
(direct interaction, pull down)
-
IRef Biogrid Interaction:
COMMD1
—
CUL2
(association, biochemical)
-
IRef Biogrid Interaction:
COMMD1
—
CUL4B
(direct interaction, pull down)
-
IRef Biogrid Interaction:
CUL1
—
CUL1
(direct interaction, pull down)
-
IRef Biogrid Interaction:
COMMD1
—
CUL5
(direct interaction, pull down)
In total, 12 gene pairs are associated to this article in curated databases