Gene interactions and pathways from curated databases and text-mining
J Proteome Res 2013, PMID: 23402259

Quantitative study of the interactome of PKCζ involved in the EGF-induced tumor cell chemotaxis.

Chen, Ruibing; Wang, Yanping; Liu, Yan; Zhang, Qing; Zhang, Xiaofang; Zhang, Fei; Shieh, Chia-Hui Paul; Yang, De; Zhang, Ning

Chemotaxis plays an important role in metastasis. In our previous studies, we reported that protein kinase C ζ (PKCζ) mediated cancer cell chemotaxis by regulating cytoskeleton rearrangement and cell adhesion. To further study the molecular mechanism of chemotaxis, mass spectrometry-based approaches were employed to investigate the interactome of PKCζ and its changes upon stimulation by epidermal growth factor (EGF). As a result, 233 proteins were identified as potential PKCζ binding partners. Label free quantification was applied to examine the quantitative changes of these interactions involved in the EGF induced chemotaxis. Fifteen identified proteins were enriched and 9 proteins were reduced in the presence of EGF (≥ 1.5 folds, p ≤ 0.05). The interaction between cofilin-1 (CFL1) and PKCζ was evidenced and this interaction was enhanced in the EGF induced chemotaxis signaling transduction. In addition, novel PKCζ interacting proteins potentially related with chemotaxis were characterized, such as isoform 1 of nucleophosmin (NPM1). Furthermore, Western blotting and chemotaxis assays were also applied to validate the proteomics result and explore its biological implications. Collectively, the combination of quantitative proteomics and biological assays provides a powerful strategy for elucidating the signaling pathway of tumor cell chemotaxis.

Diseases/Pathways annotated by Medline MESH: Breast Neoplasms
Document information provided by NCBI PubMed

Text Mining Data

Dashed line = No text mining data

Manually curated Databases

  • IRef Biogrid Interaction: YBX1 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: C1QBP — PRKCZ (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: UQCRH — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PSMC4 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: NPM1 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EIF3E — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: NPM3 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: GAPDH — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CLTC — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: BAG2 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CCT2 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: MRPS26 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PRKCA — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: KIF11 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: NPM1 — RPL10 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: MRPL12 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: NPM1 — PPP1CC (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: COPE — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: MRPS30 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RNH1 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RPS5 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: NPM1 — SRRT (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DDX3X — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: C1QBP — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: NPM1 — PSMC5 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: NPM1 — DZIP1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: ICT1 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DICER1 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: JAK1 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: NPM1 — MRPL43 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: NPM1 — PRKCZ (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: UNC5C — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: STK38 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EEF1A1 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: MRPL50 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TRIM21 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: NPM1 — TXN (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: ARHGAP5 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: NUDT21 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: MRPL46 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CFL1 — PRKCZ (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PTPN14 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: NUDCD1 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: LAMB2 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: MRPL53 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: LGALS1 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PSMD4 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CHCHD6 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EEF1B2 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EEF1D — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SNRPD1 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: NPM1 — MRPL52 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SNRPD2 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: NPM1 — BANP (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: UTRN — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PSME3 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: ATP5A1 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CHCHD4 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TEX15 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EIF3M — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EIF3H — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: NPM1 — MRPL39 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: GRWD1 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: NPM1 — GOLGA3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: MYCBP — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: KCTD17 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: IMPG1 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: AKAP11 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EEF1G — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: NPM1 — HSP90AA1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EEF2 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: MRPL19 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: S100A10 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RCOR1 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PARD6B — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: MRPL28 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DNAJC13 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TUFM — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PRKCI — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PPP2CA — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: COPB1 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TAB2 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: VASP — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: FASN — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: POLR2E — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TUBB3 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: BOLA2, BOLA2B — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TAB1 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: COPG1 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: IPO5 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EIF4A1 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TPR — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: KCTD5 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: FLNB — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: MRPL24 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: NPM1 — OSBPL1A (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CHCHD3 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: MRPL37 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: FLNC — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PSMD12 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PRKAR1A — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PKM — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DHX15 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: NPM1 — FLNA (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PARK7 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: GRN — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: NPM1 — USP15 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: MAP1B — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: IMPDH2 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SDPR — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PRDX6 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: MRPL49 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TRRAP — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: VCP — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RPS28 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: IPO9 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: MRPL54 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: ENO1 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: NPM1 — ANXA2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: NIPSNAP1 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: MRPL44 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TAF3 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CTTN — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: AGR2 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CEP152 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SLC25A5 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: LLGL1 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: NPM1 — CFL1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RPS12 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: KTN1 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PYGB — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EIF2S1 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DNAJA1 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PRDX4 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: S100A11 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EPAS1 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TUBA1C — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: FGD6 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SNRPF — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: NPM1 — HSP90AB1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DNAJA2 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PROS1 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SPIN1 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: KCTD2 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SLC25A3 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PPA1 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DSTN — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DOCK4 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PSMD1 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: KDR — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: MRPL18 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TTC27 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HSPB1 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: MRPL10 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TCP1 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: GID8 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: NPM1 — SAMM50 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RPLP1 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: NPM1 — RANBP6 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: MRPL11 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: KEAP1 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PPM1G — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EIF3I — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: WDR26 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HSPA5 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: MRPL38 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TRIM28 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: GOLGA2 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PTRF — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SQSTM1 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EIF5A — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EIF3G — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HNRNPA0 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: NPM1 — PCBP1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PPIL4 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SLAIN2 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RBBP4 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: NPM1 — CALU (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TXNDC12 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: ELMO2 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CSNK2B — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HSPA4 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: NPM1 — RPLP0 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CDC37 — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: YWHAZ — NPM1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: YWHAE — NPM1 (physical association, affinity chromatography technology)
In total, 187 gene pairs are associated to this article in curated databases