Gene interactions and pathways from curated databases and text-mining

◀ Back to PRKAR2A

PRKAR1A — PRKAR2A

Pathways - manually collected, often from reviews:

Protein-Protein interactions - manually collected from original source literature:

Studies that report less than 10 interactions are marked with *

Text-mined interactions from Literome

Uzlaner et al., J Physiol 1999 : 4. Activation of PKC by phorbol 12-myristate, 13-acetate ( TPA ) had little effect on CBF or on [Ca2+ ] i, while activation of PKA by forskolin or by dibutyryl-cAMP led to a small rise in CBF without affecting [Ca2+ ] i. 5
Tyson et al., Endocrinology 1999 : PTH increases the level of phosphorylation of CREB at S133 in a time- and dose dependent manner, correlating with the time and level of activation of PKA in response to PTH ... Taken together, these data provide strong evidence that PKA is the enzyme responsible for phosphorylating CREB at S133 in response to PTH and that PKA activity is required for PTH induced c-fos expression
Hayden et al., J Surg Res 1999 : Positive effects were then tested in the presence of KT5720 ( 10 ( -9 ) -10 ( -5 ) M ), the pharmacological inhibitor of PKA , and KT5823 ( 10 ( -9 ) -10 ( -5 ) M ), the pharmacological inhibitor of PKG. NO production was measured using the Greiss reaction, and mRNA abundance of the inducible NO synthase (iNOS) , using semiquantitative RT-PCR
Nishizaki et al., Brain Res Mol Brain Res 1999 (Ion Channel Gating) : In contrast, the protein synthesis inhibitor, cycloheximide, the selective inhibitor of cAMP dependent protein kinase (PKA) , H-89, the selective inhibitors of protein kinase C ( PKC ), PKCI and GF109203X, the mitogen activated protein ( MAP ) kinase kinase inhibitor , PD98059, or the inactive CaMKII inhibitors, KN-92 and NP218, had no effect on the currents
Poluektova et al., Immunopharmacology 1999 : In this report we explore the role of PKA in histamine mediated effects on IL-2 mRNA expression and IL-2 protein secretion
Bryce et al., Immunopharmacology 1999 : It has been demonstrated that the inhibitory effects of pharmacological elevations in cAMP on human T cell proliferation and IL-2 production do not require PKA activity
Xing et al., J Biol Chem 1999 : Taken together, these results indicate that negative feedback regulation via cAMP/PKA mediated inhibition of mitogen activated protein kinase occurs for some, but not all, classes of receptors that promote PLA2 activation and AA release
Matsushita et al., J Biol Chem 1999 : In intact PC12 cells, activation of PKA with forskolin resulted in a rapid inhibition of both CaMKK and CaMKI activity ... In intact PC12 cells, activation of PKA with forskolin resulted in a rapid inhibition of both CaMKK and CaMKI activity ... These results indicate that in vitro and in intact cells the CaMKK/CaMKI cascade is subject to inhibition by PKA mediated phosphorylation of CaMKK
Yoshii et al., Nihon Yakurigaku Zasshi 1998 : The depression was caused by activation of PTX-sensitive G-protein regulated cAMP dependent protein kinase (PKA) with subsequent phosphorylation of the ACh receptors ; in contrast, the enhancement was caused by activation of Ca ( 2+ ) -dependent protein kinase C ( PKC ) and the ensuing PKC phosphorylation of the receptors
Fliss et al., Mol Endocrinol 1999 : Transient transfection employing PREB negative C6 rat glial cells showed that PREB is as active as, and additive with, Pit-1 in transactivation of a PRL promoter construct, and that PREB, but not Pit-1 , can mediate transcriptional activation by protein kinase A (PKA)
Han et al., Endocrinology 1999 : The results show that MAPK can be activated in a PKA dependent manner in GGH ( 3 ) 1 ' cells
Igami et al., Jpn J Physiol 1999 : These results suggest that the protein, except for the SR Ca2+ release channel, is phosphorylated by endogenous PKA and plays a modulatory role in depolarization induced Ca2+ release, that is, excitation-contraction coupling of the skeletal muscle
MacNicol et al., J Biol Chem 1999 : PKA can both prevent Ras dependent Raf-1 activation and directly inhibit Raf-1 catalytic activity ... This potentiation, rather than inhibition, of PC12 cell differentiation is curious in light of the ability of PKA to inhibit Raf-1 catalytic activity ... The kinase domains of Raf-1 and B-Raf are highly conserved, and it has been predicted that B-Raf catalytic activity would also be inhibited by PKA ... We report that nerve growth factor stimulated B-Raf activity is not inhibited by PKA ... By contrast, an N-terminally truncated, constitutively active form of B-Raf is inhibited by PKA both in vitro and in transfected PC12 cells
Kiroytcheva et al., Kidney Int 1999 : In oxygenated MTALs, PKA mediated stimulation of Na+, K ( + ) -ATPase likely participates in the cAMP dependent stimulation of sodium reabsorption
Barbier et al., J Biol Chem 1999 : Because cAMP activates the cAMP dependent protein kinase A (PKA), we investigated the effect of PKA on the EGFR
Padma et al., Prostaglandins Leukot Essent Fatty Acids 1999 : AA, EPA and DHA decreased the activity of protein kinase A (PKA) both in the cytosol and particulate fractions whereas ALA and GLA enhanced the PKA activity in the particulate fractions ; all the fatty acids except ALA reduced cyclic AMP levels and an enhanced phosphorylation of about 13 proteins of the nuclear fraction and about eight proteins of the plasma membrane fraction was noted in c-UFA treated AK-5 cells in vitro
Roberson et al., J Neurosci 1999 : In the present studies we observed that hippocampal MAPK activation is regulated by both the PKA and PKC systems ; moreover, we found that a wide variety of neuromodulatory neurotransmitter receptors ( metabotropic glutamate receptors, muscarinic acetylcholine receptors, dopamine receptors, and beta-adrenergic receptors ) couple to MAPK activation via these two cascades
Jemtland et al., J Bone Miner Res 1999 (Osteosarcoma) : Here we have characterized alternative PTHrP mRNA 3 ' splicing variants, encoding PTHrP isoforms of 139, 141, and 173 amino acids, and studied the regulation of PTHrP and its mRNAs by activated PKA and PKC in two human osteoblast-like cell lines ( KPDXM and TPXM ) ... Activation of PKA or PKC resulted in different effects on PTHrP and its mRNAs in the two cell lines
Filippa et al., Mol Cell Biol 1999 : In this report, we show that PKB can also be activated by PKA ( cyclic AMP [ cAMP ] -dependent protein kinase ) through a PI3-kinase independent pathway ... Although this activation required phosphorylation of PKB, PKB is not likely to be a physiological substrate of PKA since a mutation in the sole PKA consensus phosphorylation site of PKB did not abolish PKA induced activation of PKB ... We looked at events downstream of PKB and found that PKA activation of PKB led to the phosphorylation and inhibition of glycogen synthase kinase-3 ( GSK-3 ) activity, a known in vivo substrate of PKB ... We looked at events downstream of PKB and found that PKA activation of PKB led to the phosphorylation and inhibition of glycogen synthase kinase-3 ( GSK-3 ) activity, a known in vivo substrate of PKB ... However, high concentrations of wortmannin did not abolish PKB activation, which demonstrates that translocation per se is not important for PKA induced PKB activation
Bugrim et al., Cell Calcium 1999 : The suggested mechanism is based on the regulation of local Ca2+ release by cyclic AMP dependent protein kinase (PKA) and relies upon two experimental observations : first, some G-protein coupled signaling pathways activate PLC and regulate adenylate cyclase at the same time, leading to IP3 production and altering PKA activity via changes in cAMP level ; second, phosphorylation by PKA alters the properties of IP3 receptor ( IP3R )
Yoshida et al., J Leukoc Biol 1999 : A PKC inhibitor, but not a PKA inhibitor, suppressed CD11b/CD18 up-regulation, and alpha-Toc slightly decreased fMLP- and oxLDL induced activation of PKC ... A PKC inhibitor, but not a PKA inhibitor, suppressed CD11b/CD18 up-regulation, and alpha-Toc slightly decreased fMLP- and oxLDL induced activation of PKC
Bernardo et al., J Clin Invest 1999 : PKA mediated inhibition of NHE3 is mediated by a PDZ motif containing protein, the Na ( + ) /H ( + ) exchanger regulatory factor ( NHE-RF )
Reddy et al., Oncogene 1999 (Teratocarcinoma) : ZPK inhibits PKA induced transcriptional activation by CREB and blocks retinoic acid induced neuronal differentiation ... ZPK inhibits PKA induced transcriptional activation by CREB and blocks retinoic acid induced neuronal differentiation ... Overexpression of ZPK in NTera-2 human teratocarcinoma cells results in inhibition of PKA induced transcriptional activation by CREB and prevents retinoic acid induced differentiation of the cells to neurons ... Overexpression of ZPK in NTera-2 human teratocarcinoma cells results in inhibition of PKA induced transcriptional activation by CREB and prevents retinoic acid induced differentiation of the cells to neurons ... Overexpression of ZPK in NTera-2 human teratocarcinoma cells results in inhibition of PKA induced transcriptional activation by CREB and prevents retinoic acid induced differentiation of the cells to neurons
Wang et al., J Biol Chem 1999 : Two PKA inhibitors, H89 and KT5720, inhibited immune complex ( IC ) -stimulated LPL phosphorylation as well as IC-induced activation of alpha ( M ) beta ( 2 ) -mediated adhesion in PMN ... These data demonstrate a necessary role for PKA in IC-induced integrin activation and LPL phosphorylation
Parissenti et al., Int J Cancer 1999 (Breast Neoplasms) : In contrast with findings in Chinese hamster ovary and Y1 adrenal cells, P-gp levels and cellular sensitivity to drugs which are P-gp substrates were unchanged in the PKA inhibited transformants, suggesting that P-gp expression and function are not under PKA dependent control in MCF-7 ( ADR ) cells
Galisteo et al., J Pharmacol Exp Ther 1999 : Taken together, our results suggest that CYP3A induction by PB is regulated positively by calmodulin dependent protein kinase and cGMP dependent protein kinase, and negatively by PKA in mouse hepatocytes in primary culture ... Taken together, our results suggest that CYP3A induction by PB is regulated positively by calmodulin dependent protein kinase and cGMP dependent protein kinase, and negatively by PKA in mouse hepatocytes in primary culture
Liu et al., Mol Cell Endocrinol 1999 (Adrenal Gland Neoplasms) : We show here that CBP enhanced the PKA induced CYP11A promoter activity, while a double mutation of both Sp1 and SF-1 sites within the CYP11A promoter region abolished CBP induced activity ... Also, CBP potentiated the transcriptional activity of GAL4-SF-1 in the presence of PKA ... Also, CBP potentiated the transcriptional activity of GAL4-SF-1 in the presence of PKA
Bode et al., Endocrinology 1999 : Another PKA inhibitor, H-89, reduced the [ Ca2+ ] cyt elevation only when applied at high concentrations ( 10-40 microM ), higher than sufficient for PKA inhibition in many cell types
Costanzo et al., Curr Biol 1999 : Inhibition of PKA activity, however, blocked the release of Cdc6 from chromatin and subsequent DNA replication
Gupta et al., J Biol Chem 1999 : To determine mechanisms underlying the interdependence of BMP signaling and PKA activity, we examined the effect of PKA on the known signaling events in the BMP-2 dependent Smad1 signaling pathway and the effect of BMP-2 on PKA activity ... PKA did not induce endogenous Smad1 phosphorylation, Smad1-Smad4 complex formation, or Smad1 nuclear translocation ... PKA did not induce endogenous Smad1 phosphorylation, Smad1-Smad4 complex formation, or Smad1 nuclear translocation ... In contrast, BMP-2 increased endogenous PKA activity and induced phosphorylation of the PKA effector, cAMP-response element binding protein, in a PKA dependent manner ... In contrast, BMP-2 increased endogenous PKA activity and induced phosphorylation of the PKA effector, cAMP-response element binding protein, in a PKA dependent manner ... We conclude that BMP-2 induces activation of PKA and that PKA regulates the effects of BMPs on collecting duct morphogenesis without activating the known signaling events in the BMP-2 dependent Smad1 signaling pathway
Choi et al., Peptides 1999 : The PACAP activated PKA up-regulated phenylethanolamine N-methyltransferase expression whereas PKC caused down-regulation
Shoge et al., Brain Res 1999 : Protection by PACAPs ( 100 nM ) against glutamate induced neurotoxicity was antagonized by PACAP6-38 ( 1 microM ), a PACAP antagonist, and H-89 ( 1 microM ), a protein kinase A (PKA) inhibitor
Gao et al., J Appl Physiol 1999 : PKA activity was not affected by 8-Br-PET-cGMPS but was inhibited by H-8 and KT-5720
Zhou et al., J Clin Endocrinol Metab 1999 : Inhibition of PKA activity by cotreatment with isoquinoline-sulfonamide ( H-89 ) prevented hCG from increasing COX-2 protein levels
Thomas et al., J Biol Chem 1999 : We investigated the role of protein kinase A (PKA) in regulation of the human ether-a-go-go related gene (HERG) potassium channel activation ... We conclude that PKA regulates HERG channel activation, and direct phosphorylation of the HERG channel protein has a functional role that may be important in regulation of cardiac repolarization
Rind et al., J Neurosci Res 1999 : These studies examined the extent to which protein kinase C ( PKC ) and cAMP dependent protein kinase (PKA) regulate the neuronal differentiation of the raphe derived neuronal cell line, RN33B
Brosens et al., Endocrinology 1999 : Human endometrial stromal ( ES ) cells in culture express PRL , a marker of decidualization, in response to sustained activation of protein kinase A (PKA)
Zhang et al., J Neurochem 1999 (Glioma...) : Inhibition of protein kinase A (PKA) strongly diminished p38 activation mediated by DOR or ORL1 but had no significant effect on ERK activation, and protein kinase C ( PKC ) inhibitors potentiated stimulation of p38 while inhibiting activation of ERKs ... Taken together, our results provide the first evidence for coupling of DOR and ORL1 to the p38 MAPK cascade and clearly demonstrate that receptor mediated activation of p38 MAPK both involves PKA and is negatively regulated by PKC
Rao et al., Exp Cell Res 1999 (Breast Neoplasms) : In the present study we examined the role of cAMP dependent protein kinase (PKA) on p21 and p27 induction in the breast cancer cell line, MDA-MB-157 ... In the present study we examined the role of cAMP dependent protein kinase (PKA) on p21 and p27 induction in the breast cancer cell line, MDA-MB-157
Noma et al., Neurosci Lett 1999 (Retinoblastoma) : On the other hand, stimulation of PKA by forskolin had a weak suppressive effect on NeuroD mRNA expression
Chen et al., J Biol Chem 1999 : The PKA inhibitors, KT-5720 and H8, reduced LPS induced NO release and inducible nitric oxide synthase (iNOS) expression ... The direct PKA activator, Bt ( 2 ) cAMP, caused concentration dependent NO release and iNOS expression, as confirmed by immunofluorescence studies ... The PKA inhibitor, H8, inhibited the NF-kappaB activation induced by 6- or 12-h treatment with LPS but not that induced after 1, 3, or 24 h
Maronde et al., J Pineal Res 1999 : Immunochemical or immunocytochemical demonstration of serine133 phosphorylated cyclic AMP regulated element binding protein ( pCREB ) and radioimmunological detection of melatonin revealed that only cyclic AMP dependent protein kinase (PKA) inhibitors suppressed NE-induced CREB phosphorylation and stimulation of melatonin biosynthesis, whereas inhibitors of cyclic GMP dependent protein kinase ( PKG ), mitogen activated protein kinase kinase, protein kinase C, or calcium-calmodulin dependent protein kinase ( CaMK ) were ineffective
Engelman et al., J Biol Chem 1999 (Cell Transformation, Viral) : We also show that activation of protein kinase A (PKA) signaling is sufficient to down-regulate caveolin-1 protein expression and promoter activity
Martel-Pelletier et al., Arthritis Rheum 1999 (Osteoarthritis) : Interestingly, IL-17 induced phosphorylation of the stress activated protein kinase/Jun N-terminal kinase ( SAPK/JNK ) ( p54/46 ) only when PKA was inhibited
Mamoon et al., J Pharmacol Exp Ther 1999 : Protein kinase A (PKA) inhibitors H-89 and KT5720 significantly decreased forskolin- and isoproterenol mediated activation of PLD activity ... These results suggest that increasing the concentration of cAMP activates PLD via activation of PKA and that the increased activity of PKA also inhibits cholinergic stimulation of PLD , in part at least by inhibiting the activation of phospholipase C by ACh ... These results suggest that increasing the concentration of cAMP activates PLD via activation of PKA and that the increased activity of PKA also inhibits cholinergic stimulation of PLD, in part at least by inhibiting the activation of phospholipase C by ACh
Carr et al., Biochem J 1999 : These results suggest that altered PKA-AKAP interactions may contribute to the distinct responses of PA and PO follicles to high levels of cAMP, and that higher cAMP levels are required to activate PKA in PO ovaries
Seibert et al., Biochim Biophys Acta 1999 (Ion Channel Gating) : Therefore, it can be concluded that ancillary phosphoproteins do not contribute to CFTR activation by PKA
Varone et al., Arch Biochem Biophys 1999 : Our data suggest that the transcription factor cAMP-response element binding protein ( CREB ) is probably involved in PKA induction of ALA-S gene expression ... Our data suggest that the transcription factor cAMP-response element binding protein ( CREB ) is probably involved in PKA induction of ALA-S gene expression ... The present work provides evidence that cAMP, through PKA mediated CREB phosphorylation, and phenobarbital induce ALA-S expression at the transcriptional level, while heme represses it
Bibb et al., Nature 1999 : Phospho-Thr 75 DARPP-32 inhibits PKA in vitro by a competitive mechanism
Pursiheimo et al., Proc Natl Acad Sci U S A 2000 : In this paper, we report that the activation of FiRE by FGF-2 requires protein kinase A (PKA) in NIH 3T3 cells ... Also, expression of a dominant negative form of PKA inhibited the FGF-2 induced FiRE activation and the transcription of the syndecan-1 gene ... In accordance with the growth factor specificity of FiRE, the activity of PKA was stimulated by FGF-2 but not by platelet derived growth factor or epidermal growth factor ... In accordance with the growth factor specificity of FiRE, the activity of PKA was stimulated by FGF-2 but not by platelet derived growth factor or epidermal growth factor ... We propose that the FGF-2-selective transcriptional activation through FiRE is caused by the ability of FGF-2 to control PKA activity
Wartenberg et al., Int J Cancer 2000 (Prostatic Neoplasms) : Down-regulation of Pgp by H ( 2 ) O ( 2 ) was abolished when either forskolin, 8-Br-cAMP or IBMX, which raise intracellular cAMP levels, was co-administered, indicating that Pgp expression is regulated by protein kinase A (PKA) ... Furthermore, Pgp was down-regulated by the PKA inhibitors Rp-cAMPs and H89
Norbeck et al., Yeast 2000 : Ymr116c was PKA independent, while Pgi1p , Sam1p, Gdh1p and Vma1p were fully PKA dependent ... Ymr116c was PKA independent, while Pgi1p, Sam1p, Gdh1p and Vma1p were fully PKA dependent
Swanson et al., J Biol Chem 2000 : The N-terminal activation domain of Arix interacts with the transcriptional co-activator, cAMP-response element binding protein binding protein, which potentiates transcription from the DBH promoter in a PKA dependent manner
Dawson et al., Biochem Cell Biol 1999 : The previous hypothesis was that PP-1 activity among several lower eukaryotes may be regulated directly by cAMP dependent protein kinase (PKA) phosphorylation
Michael et al., Mol Cell Biol 2000 : Cyclic AMP ( cAMP ) stimulates the expression of numerous genes via the protein kinase A (PKA) mediated phosphorylation of CREB at Ser133
Du et al., Cell Immunol 2000 : Inhibition of PKC and PKA activation results in suppression of activation and translocation of NF-kappaB
Rahn Landström et al., Biochem J 2000 : In addition, the PKA activation induced by dbcAMP resulted in the down-regulation of PDE3B
Vitalis et al., Proc Natl Acad Sci U S A 2000 : In fact, pharmacological inhibition of PKA activity caused an increase in the basal secretion of GnRH ... Consistent with this observation activation PKA inhibited adenylyl cyclase activity, presumably by inhibiting adenylyl cyclase V expressed in the cells
Kishi et al., Br J Pharmacol 2000 : Thus, the present study suggests that activation of PKA via activation of VIP receptors is associated with activation of ChTx-sensitive K ( + ) channels in relaxation of longitudinal muscle of the distal colon of Wistar-ST rats
Ogawa et al., Mol Reprod Dev 2000 : Dibutyryl cAMP ( dbcAMP ), a protein kinase A (PKA) activator, added to the culture medium increased the phosphorylation of Cx43 and Cx43 positive spots
Howard et al., Toxicol Appl Pharmacol 2000 (Cholestasis, Intrahepatic) : Interestingly, although it is classically viewed as an initiator of the adenylate cyclase/protein kinase A (PKA) pathway, PKA inhibition did not inhibit forskolin 's activation of FXR nor was cyclic AMP ( cAMP ) able to stimulate FXR mediated transcription
Nakazawa et al., Biochem Biophys Res Commun 2000 (Joint Diseases) : Furthermore, PK-A activation induced CBP-Tax complex in synoviocytes from HAAP and the complex contained CREB ... Furthermore, PK-A activation induced CBP-Tax complex in synoviocytes from HAAP and the complex contained CREB
Gavaravarapu et al., Biochem Biophys Res Commun 2000 : Overexpression of Tip results in an almost complete block in activation of a Gal4-CREB ( cAMP response element binding protein ) fusion protein by cyclic AMP dependent protein kinase A (PKA) ... Overexpression of Tip results in an almost complete block in activation of a Gal4-CREB ( cAMP response element binding protein ) fusion protein by cyclic AMP dependent protein kinase A (PKA)
Nardini et al., Biochim Biophys Acta 2000 : PBE at the concentration of 20 microg/ml ( corresponding to 69 microM catechin equivalents ) inhibited PKA , PhK and PKC by about 90, 59, 57 %, respectively, while 100 microM CA inhibited by 37, 52 and 54 %, respectively
Beard et al., J Biol Chem 2000 : Mutation of the PKA substrate site in UCR1 ( Ser-54 ) to aspartic acid, which mimics the activation of PDE4D3 by PKA , profoundly reduced the interaction between UCR1 and UCR2
Tzavara et al., Eur J Neurosci 2000 (Substance Withdrawal Syndrome) : We show that after SR141716A precipitation of cannabinoid withdrawal, basal and calcium-calmodulin stimulated adenylyl cyclase activities as well as active PKA in the cerebellum increase in a transient manner with a temporal profile which matches that of the somatic expression of abstinence
Streeper et al., J Biol Chem 2000 : However, in LLC-PK cells, but not in HepG2 cells, a hepatocyte nuclear factor-1 ( HNF-1 ) binding site was critical for the full induction of G6Pase-CAT expression by PKA ... However, in LLC-PK cells, but not in HepG2 cells, a hepatocyte nuclear factor-1 ( HNF-1 ) binding site was critical for the full induction of G6Pase-CAT expression by PKA ... Changing this HNF-1 motif to that for the yeast transcription factor GAL4 reduces the PKA response in LLC-PK cells to the same degree as deleting the HNF-1 site
Vianna et al., Behav Pharmacol 1999 (Amnesia, Retrograde) : The role of PKA in STM may well involve other substrates of the enzyme
Sun et al., J Biol Chem 2000 : Phosphorylation reactions mediated by CFTR associated PKA II are inhibited by Ht31 peptide but not by the control peptide Ht31P, indicating that a protein kinase A anchoring protein ( AKAP ) is responsible for the association between PKA and CFTR ... Taken together, these data demonstrate that PKA II is linked physically and functionally to CFTR by an AKAP interaction, and they suggest that ezrin serves as an AKAP for PKA mediated phosphorylation of CFTR
Figiel et al., J Neurosci 2000 : Although PAC1 receptor dependent activation of protein kinase A (PKA) was sufficient to promote the expression of GLAST, the activation of both PKA and protein kinase C ( PKC ) was required to promote GLT-1 expression optimally
Huang et al., Mol Cell Biol 2000 (Chondrosarcoma) : In COS-7 cells cotransfected with PKA-Calpha and SOX9 expression plasmids, PKA enhanced the phosphorylation of wild-type SOX9 but did not affect phosphorylation of a SOX9 protein in which the two PKA phosphorylation sites ( S ( 64 ) and S ( 211 ) ) were mutated
Du et al., Mol Cell Biol 2000 : Like protein kinase A (PKA) stimulated wild-type CREB , the Tyr134Phe polypeptide was found to stimulate target gene expression via the Ser133 dependent recruitment of CBP and p300
Zheng et al., J Biol Chem 2000 : In summary, activation of PKA or PKC increases apoAI promoter activity
Yaguchi et al., FEMS Microbiol Lett 2000 : Studies using mutants of protein kinase A (PKA) and CDC25, the Ras-GTP exchange activator, showed that PKA positively regulated the phosphorylation level of Gts1p
Martin et al., J Biol Chem 2000 : C-PKA also stimulated recruitment and activation of a recombinant form of human ARF1 in the absence of additional cytosolic components
Murakami et al., J Periodontal Res 2000 (Periodontitis) : Adenosine induced IL-6 production was suppressed by protein kinase A (PKA) inhibitor, H89, indicating that cAMP/PKA pathway is involved in the induction
Chen et al., Br J Pharmacol 2000 : Moreover, the cyclic AMP/PKA dependent up-regulation of iNOS expression mediated by PGE ( 2 ) was drawn from the inhibitory effects of 2',5'-dideoxyadenosine, KT5720 and H-89
Lee et al., Neurosci Lett 2000 : It is not clear, however, whether PKA acts directly on the capsaicin-sensitive channel that is responsible for I(CAP)
Han et al., Biochem Biophys Res Commun 2000 : Previous results from our laboratory demonstrated that water channel activity of AQP1 was significantly increased by protein kinase A (PKA) activators such as cyclic-AMP ( cAMP ) and forskolin
Ding et al., Cytokine 2000 : Both inhibitors and activators for PKA and G-protein as well as IL-10 had no effects on IL-6 expression
Usukura et al., Genes Cells 2000 : The second messenger cAMP stimulates the expression of numerous genes through the PKA dependent phosphorylation of CREB
Vinade et al., Cell Mol Neurobiol 2000 : Prior incubation of PSDs under dephosphorylating conditions results in only a small PKA mediated phosphorylation of GluR1
Hirota et al., Osteoarthritis Cartilage 2000 : Activation of PKA by 8-bromo-cyclic AMP or forskolin, and inhibition of PKC by calphostin C, resulted in a significant decrease in 3TP activity as well as in vitro ERK kinase activity in CRAC
Singh et al., J Investig Med 2000 : GlcN inhibits insulin action on both PKA and CK-1 activities
Park et al., Neurosci Lett 2000 : Here we demonstrate that PKA is required for depolarization induced activation of both extracellular signal regulated kinase ( ERK ) and p38 mitogen activated protein ( MAP ) kinase in PC12 cells ... Here we demonstrate that PKA is required for depolarization induced activation of both extracellular signal regulated kinase ( ERK ) and p38 mitogen activated protein ( MAP ) kinase in PC12 cells ... In addition, we have found that the depolarization induced tyrosine phosphorylation of proline-rich tyrosine kinase (PYK) 2 , a key calcium-sensitive upstream mediator of MAP kinase activation, is profoundly blocked by PKA inhibition ... In contrast to the depolarization induced signaling, the ERK and PYK2 activation by bradykinin ( 1 microM ), a G-protein coupled receptor agonist, was not blocked by PKA inhibition ... In contrast to the depolarization induced signaling, the ERK and PYK2 activation by bradykinin ( 1 microM ), a G-protein coupled receptor agonist, was not blocked by PKA inhibition ... These findings suggest that PKA inhibition prevents depolarization induced PYK2/MAP kinase pathway activation, thereby inhibiting the early gene expression
Gupta et al., Nucleic Acids Res 2000 : We also show that PK-A response of the alpha-MHC gene is stimulated by the presence of wild-type TEF-1 but not by mutant TEF-1 having serine-102 replaced by alanine, suggesting that phosphorylation at this residue accounts for the cAMP/PK-A response of the gene
Yan et al., J Cell Physiol 2000 : Therefore, TGF-beta1 may activate PKA to inhibit the EGF activated MEK1-MAPK pathway
Lee et al., Oncogene 2000 (Lung Neoplasms) : Furthermore, PKA activators forskolin and dibutyryl-cAMP activated p21WAFI promoter activity and increased p21wAF1 protein level in lung cancer cells ... Taken together, these results demonstrate that Tam activates the p21WAF1 promoter via Sp1 binding sites and suggest that PKA may be involved in the induction of p21wAF1 by Tam in ER-negative lung cancer cells
Grewal et al., J Biol Chem 2000 : This pathway involves the PKA dependent activation of the Ras related small G-protein, Rap1 , and subsequent stimulation of the neuronal Raf isoform, B-Raf
Miyashita et al., Receptors Channels 2000 (MAP Kinase Signaling System) : Taken together, we concluded that the Ca2+o sensing function of mGluR1alpha and the continuous stimulation by Ca2+o caused the increase in the basal cAMP level by direct coupling with Gs, and triggered the subsequent activation of PKA , MAPKK, and MAPK cascade which resulted in the morphological change of transfected CHO cells
Csanády et al., J Gen Physiol 2000 (Cystic Fibrosis...) : Split channels with no R domain ( from coexpression of CFTR segments 1-633 and 837-1480 ) were highly active without phosphorylation, but otherwise displayed the characteristics of channels cut after the R domain, including higher apparent ATP affinity, and less tight binding of AMPPNP at the locking site, than for WT. Intriguingly, severed channels with no R domain were still noticeably stimulated by PKA, implying that activation of WT CFTR by PKA likely also includes some component unrelated to the R domain
Makarevich et al., Biol Signals Recept 2000 : The role of cAMP/protein kinase A (PKA)- and tyrosine kinase ( TK ) -dependent intracellular mechanisms in mediating the action of porcine growth hormone (GH) on insulin-like growth factor I (IGF-I) secretion by porcine ovarian granulosa cells was studied ... The stimulation of PKA by the addition of either a cAMP agonist or a phosphodiesterase inhibitor to the medium increased IGF-I release by the cells, indicating a direct stimulation of IGF-I release by cyclic nucleotides
Frost et al., Endocrinology 2000 : Conversely, a PKA inhibitor ( H-89 ) inhibited the ability of cAMP, but not IL-1beta to stimulate IGFBP-1 synthesis
Weinman et al., American journal of physiology. Renal physiology 2000 : Signal complex regulation of renal transport proteins : NHERF and regulation of NHE3 by PKA ... This article reviews studies on the regulation of NHE3 by NHERF, PKA , and ezrin and introduces the concept of regulation of renal transporters by signal complexes
Garreau et al., Microbiology 2000 : These results suggest that PKA and stress activated kinases control Msn2/4p activity by antagonistic phosphorylation
Zhu et al., Curr Genet 2000 : However, MSI1 does not inhibit cAMP synthesis or total cellular cAMP dependent protein kinase (PKA) activity, nor does MSI1 stimulate expression of several cAMP-repressible genes critical for the acquisition of thermotolerance in the stationary phase ... However, MSI1 does not inhibit cAMP synthesis or total cellular cAMP dependent protein kinase (PKA) activity, nor does MSI1 stimulate expression of several cAMP-repressible genes critical for the acquisition of thermotolerance in the stationary phase ... These observations indicate that MSI1 affects PKA function in a BCY1 dependent manner via mechanisms other than direct overall inhibition of PKA catalytic activity
Delgado et al., J Immunol 2000 : The effects of VIP/PACAP are mediated through the specific VIP/PACAP receptor-1 and the cAMP/protein kinase A (PKA) transduction pathway, but not through the induction of suppressor of cytokine signaling-1 or suppressor of cytokine signaling-3
Howe et al., Nat Cell Biol 2000 (MAP Kinase Signaling System) : Furthermore, PKA negatively regulates PAK in vivo, and whereas the induction of anchorage independent signaling resulting from PKA suppression is blocked by dominant negative PAK, it is markedly prolonged by constitutively active PAK
Kaesler et al., Biol Chem 2000 : It was found that PKA inhibits GLI1 transcriptional activity ... Mutation of Ser336 revealed that PKA could also stimulate GLI1 transcriptional activity ... Thus, our data demonstrate both negative and positive regulation of human GLI1 by PKA
Zakhary et al., J Biol Chem 2000 : PKA stimulation of intact Chinese hamster ovary cells increased RIIalpha binding to AKAP100/mAKAP and AKAP15/18 by approximately 7- and 82-fold, respectively ... PKA stimulation of intact Chinese hamster ovary cells increased RIIalpha binding to AKAP100/mAKAP and AKAP15/18 by approximately 7- and 82-fold, respectively
Zhang et al., Cell Immunol 2000 : The results in this paper indicate that LPS induced NF-kappa B binding activity was independent of PKC, PKA , ERK, and p38 MAPK, but was regulated by proteasome
Knöfler et al., Endocrinology 2000 : We conclude that differentiation dependent transcription of the CGalpha gene in villous trophoblasts is mainly governed by increasing expression of AP-2alpha and PKA dependent phosphorylation of CREB-1 and ATF-1 ... We conclude that differentiation dependent transcription of the CGalpha gene in villous trophoblasts is mainly governed by increasing expression of AP-2alpha and PKA dependent phosphorylation of CREB-1 and ATF-1
Welton et al., Genetics 2000 : PKA inhibits glucose starvation induced processes, such as conjugation and meiosis, and the transcription of the fbp1 gene that encodes the gluconeogenic enzyme fructose-1,6-bisphosphatase
Baouz et al., J Biol Chem 2001 : We could demonstrate the involvement of cAMP dependent protein kinase (PKA) in the phosphorylation of CDC25Mm in fibroblasts overexpressing this RasGEF as well as in mouse brain synaptosomal membranes
Zheng et al., J Biol Chem 2000 : In contrast, a specific peptide inhibitor of PKA, PKI ( 5 microm ), completely abolished the stimulatory effect of beta ( 2 ) -AR, suggesting that beta ( 2 ) -AR induced p38 MAPK activation is mediated via a PKA dependent mechanism, rather than by G ( i ) or Gbetagamma ... In contrast, a specific peptide inhibitor of PKA, PKI ( 5 microm ), completely abolished the stimulatory effect of beta ( 2 ) -AR, suggesting that beta ( 2 ) -AR induced p38 MAPK activation is mediated via a PKA dependent mechanism, rather than by G ( i ) or Gbetagamma
Kurihara et al., Am J Physiol Cell Physiol 2000 : These results show that Na ( + ) -K ( + ) -ATPase of rat parotid gland acinar cells is regulated in vivo by membrane anchored PKA via AKAP rather than by free cytosolic PKA
Gaudiot et al., Am J Physiol Cell Physiol 2000 : The DPI effect on PKA activity was prevented by SNAP , ascorbate, or DDC ... The DPI effect on PKA activity was prevented by SNAP, ascorbate, or DDC
Feoktistov et al., Mol Pharmacol 2000 : Protein kinase A (PKA) mediated phosphorylation is likely involved in cAMP dependent Cdc42 activation, because transient expression of the PKA catalytic subunit in COS-7 cells activated Cdc42
Nishi et al., Proc Natl Acad Sci U S A 2000 : This effect of dopamine is achieved through dopamine D1 receptor mediated activation of cAMP dependent protein kinase (PKA)
Koch et al., Br J Pharmacol 2000 : Similarly, neither pertussis toxin ( PTX ) nor RP : -8-Br-cAMPS, an inhibitor of cyclic AMP dependent protein kinase (PKA) , affected basal pERK levels or antagonized the inhibitory effect of procaterol
Chiang et al., J Clin Endocrinol Metab 2000 : Furthermore, our data suggest that the effects of hCG and GnRH on ERalpha and ERbeta expression in hGLCs are mediated in part by activation of PKA and PKC signaling pathways, respectively ... Furthermore, our data suggest that the effects of hCG and GnRH on ERalpha and ERbeta expression in hGLCs are mediated in part by activation of PKA and PKC signaling pathways, respectively ... Furthermore, our data suggest that the effects of hCG and GnRH on ERalpha and ERbeta expression in hGLCs are mediated in part by activation of PKA and PKC signaling pathways, respectively
Lamb et al., Mol Cell Biol 2000 (Breast Neoplasms) : Further, we show that culture of MCF-7 cells on a cellular substratum of murine preadipocytes also enhanced the functional interaction between cyclin D1 and ER in a PKA dependent manner
Sirotkin et al., J Steroid Biochem Mol Biol 2000 : In a second series of experiments, the effects of Rp-8-Br-PET-cGMPS ( 50 nM ) and KT5822 ( 100 ng/ml ), specific inhibitor of cGMP dependent protein kinase ( PKG ), on cAMP, PKA , oxytocin and the occurrence of apoptosis in cultured cells were compared
Rocchi et al., Biochem J 2000 : Furthermore, using an in vitro kinase assay, we showed that SHP2 was a target for cAMP dependent protein kinase (PKA)
Bozdagi et al., Neuron 2000 (Synaptic Transmission) : The increases in synaptic number and N-cadherin levels are dependent on cAMP dependent protein kinase (PKA) and protein synthesis, both of which are also required for L-LTP
Sekar et al., Endocrinology 2000 : Together, these investigations document a significant role for insulin or IGF-I in enhancing LH-stimulated progesterone and cAMP biosynthesis and endogenous StAR message and protein accumulation and in augmenting cAMP-PKA dependent transcriptional activation of the exogenous StAR promoter
Yoon et al., J Bone Miner Res 2000 : The inhibition of PKA did not affect expression of protein kinase C-alpha (PKCalpha) , PKCepsilon, PKCdelta, and PKClambda/iota, which are the isoforms expressed in differentiating mesenchymal cells ... However, PKA inhibition completely blocked activation of PKCalpha ... Because PKC activity regulates N-cadherin expression and chondrogenesis, the PKA mediated regulation of PKCalpha appears to be responsible for the PKA regulation of N-cadherin expression and chondrogenesis ... Because PKC activity regulates N-cadherin expression and chondrogenesis, the PKA mediated regulation of PKCalpha appears to be responsible for the PKA regulation of N-cadherin expression and chondrogenesis ... Taken together, our results suggest that PKA regulates chondrogenesis by activating PKCalpha at the stage of post-precartilage condensation
Riese et al., Mol Cell Biochem 2000 (Acute Disease...) : In contrast to monocytic cells, in T-cells cAMP induced PKA dependent phosphorylation of the CRE binding protein 1 (CREB-1) seems to play a marginal role in IL-10 induction, which was reflected by a low cAMP dependent IL-10-promoter/enhancer stimulation in reporter gene assays
Lazennec et al., Mol Endocrinol 2000 : We show here that activators of protein kinase A (PKA) can enhance mouse PPAR activity in the absence and the presence of exogenous ligands in transient transfection experiments ... Moreover, gel retardation experiments suggest that PKA stabilizes binding of the liganded PPAR to DNA
Kühne et al., Oncogene 2000 : In contrast, the degradation of p53 induced by E6 is not affected by PKA
Zanassi et al., J Biol Chem 2001 : We have found that PKA induced CREB phosphorylation and CREB dependent transcription are mediated by calcium ( Ca ( 2+ ) ) release from intracellular stores and are blocked by inhibitors of the protein kinase C and ERK pathways ... These results suggest that, in striatal neurons, intracellular Ca ( 2+ ) release, Rap1, and ERK pathway play a crucial role in the PKA induced CREB phosphorylation and CREB dependent transcription
Feliciello et al., Cell Growth Differ 2000 : We asked whether PKA directly activates TTF1 ... We show that cAMP/PKA activates pTg and a synthetic target promoter carrying TTF1 binding site repeats in several cell types ... However, alanine substitution at this residue or at all sites of phosphorylation did not reduce PKA activation of pTg ... Thus, PKA stimulates TTF1 transcriptional activity in an indirect manner, perhaps by recruiting to or removing from the target promoter another regulatory factor ( s )
Felinski et al., Mol Cell Biol 2001 : The nuclear extracts used mediated protein kinase A (PKA)-inducible transcription, but phosphorylation of CRG ( both of the CREB activation domains fused to the Gal4 DNA binding domain ) or KID-G4 did not mediate recruitment of a complex, and mutation of the PKA site in CRG abolished transcription induction by PKA but had no effect upon recruitment
Li et al., Mol Cell Biol 2001 : In the present study using both in vivo and in vitro analysis, we found that LRP phosphorylation is mediated by the cAMP dependent protein kinase A (PKA)
Tetradis et al., Endocrinology 2001 : However, inhibition of the PKA but not the PKC pathway significantly inhibited the PTH induction of Nurr1 ... However, inhibition of the PKA but not the PKC pathway significantly inhibited the PTH induction of Nurr1
Watson et al., Insect Biochem Mol Biol 2001 : PKI had no effect on the ability of ADP to slow rundown, suggesting that the beta-phosphate of ADP is not involved in PKA dependent phosphorylation of the GABA receptor
Dautzenberg et al., Neuropharmacology 2001 (Retinoblastoma) : These experiments demonstrate that GRK3 plays an important role in the homologous desensitization of retinoblastoma PAC(1) receptors, whereas PKC, but not PKA , contributes to the heterologous desensitization of retinoblastoma PAC(1) receptors
Fink et al., Circ Res 2001 : The increase in PKA dependent phosphorylation of troponin I and myosin binding protein C on isoproterenol stimulation was significantly reduced in Ht31 expressing cells compared with controls
Shioda et al., Ann N Y Acad Sci 2000 (Calcium Signaling) : An inhibitor of protein kinase A (PKA) completely inhibited the PACAP induced increase in [ Ca2+ ] i, but only partly inhibited the NA-induced increase in [ Ca2+ ] i
Paz et al., Endocr Res 2000 : In adrenal cortex, ACTH regulation of steroidogenesis depends on PKA dependent serine/threonine phosphorylation and also on the activity of protein tyrosine phosphatases (PTPs) ... Serine/threonine phosphorylation of these enzymes themselves could be a regulatory mechanism of their activity since the increase of total PTP activity is dependent on PKA-activation
Patterson et al., Endothelium : journal of endothelial cell research 2000 : Although PKA activation attenuated thrombin induced myosin light chain ( MLC ) phosphorylation, PKA inhibition per se did not cause MLC phosphorylation or affect [ Ca2+ ] i
Ryder et al., J Bone Miner Res 2001 (Calcium Signaling...) : Activation of either the protein kinase C ( PKC ) or protein kinase A (PKA) pathways with specific agonists indicated that PKC activation did not alter the Ca2+ response to shear, whereas PKA activation significantly increased the [ Ca2+ ] i response to lower magnitudes of shear
Lei et al., J Tongji Med Univ 2000 (Adenoma...) : In order to investigate the relationship between abnormal intracellular signal transduction and tumorgenesis of human pituitary somatotrophinomas, the effects of protein kinase A (PKA) dependent growth hormone (GH) releasing hormone ( GHRH ) and protein kinase C ( PKC ) -dependent GH-releasing peptide ( GHRP-6 ) on cAMP production were observed by using cell culture and biochemical methods, and the expression of the gsp oncogene was detected by using PCR and direct sequence assay methods in 11 patients with human pituitary somatotrophinomas
Janoo et al., Genetics 2001 (MAP Kinase Signaling System) : The CBF appears to act in parallel to atf1-pcr1, although it is unclear whether or not CBF activity is regulated by PKA
Piech-Dumas et al., J Neurochem 2001 : TPA treatment results in phosphorylation of cAMP responsive element binding protein ( CREB ) and activation of cAMP dependent protein kinase (PKA) in PC12 cells ; hence, we tested whether CREB and/or PKA are essential for the TPA response
Ortmeyer et al., J Nutr 2001 (Diabetes Mellitus, Type 2...) : The insulin effect ( insulin stimulated minus basal ) on the G6P Ka of GS was strongly positively related to the insulin effect on G6P content ( r = 0.80, P : < 0.0001 ) across the entire group of monkeys
Tsygankova et al., Mol Cell Biol 2001 : Both cAMP and protein kinase A (PKA) contribute to the regulation of Rap1 activity and signaling by TSH ... Interference with PKA activity blocked phosphorylation but not the activation of Rap1 ... The dual regulation of Rap1 by cAMP and PKA extends to downstream effectors ... Although the expression of activated Rap1A was sufficient to stimulate wortmannin-sensitive Akt phosphorylation, TSH further increased Akt phosphorylation in a phosphatidylinositol 3-kinase- and PKA dependent manner
Tai et al., J Neurochem 2001 : Forskolin treatment of PC12 cells also rapidly increased nuclear levels of Egr-1 and the catalytic subunit of PKA ( PKA-C ), with the rise in PKA-C preceding that of Egr-1
Tang et al., Acta Pharmacol Sin 2000 : To study the effects of dopamine ( DA ) and glutamate ( Glu ) and their receptor agonists/antagonists on Ca2+/calmodulin dependent protein kinase II ( CCDPK II ), cyclic AMP dependent protein kinase A (PKA) activities and the LDH release in rat striatal slices, and to examine the interaction between DA and Glu transmitter systems in striatum ... To study the effects of dopamine ( DA ) and glutamate ( Glu ) and their receptor agonists/antagonists on Ca2+/calmodulin dependent protein kinase II ( CCDPK II ), cyclic AMP dependent protein kinase A (PKA) activities and the LDH release in rat striatal slices, and to examine the interaction between DA and Glu transmitter systems in striatum
Cong et al., J Biol Chem 2001 : Mutants of AKAP79 that do not bind PKA or target to the beta2AR markedly inhibit phosphorylation of beta2AR/PKA-
Billiard et al., J Biol Chem 2001 : We thus define a pathway for PKA mediated activation of C/EBPdelta through its regulated nuclear import
Dulubova et al., J Neurochem 2001 : In non-neuronal cells, ARPP-19 was highly phosphorylated in response to activation of PKA ... In non-neuronal cells, ARPP-19 was highly phosphorylated in response to activation of PKA
Adams et al., J Neurochem 2001 : In addition, eticlopride did not produce measurable levels of PKA activity in striatum, whereas the cAMP activator Sp-8-Br-cAMPs increased levels of activated PKA
Chheda et al., Nat Cell Biol 2001 : PKA-phosphorylation of Snapin significantly increases its binding to synaptosomal associated protein-25 ( SNAP-25 )
Dodge et al., EMBO J 2001 : Disruption of PKA- mAKAP interaction prevents this enhancement of PDE4D3 activity, suggesting that the proximity of both enzymes in the mAKAP signaling complex forms a negative feedback loop to restore basal cAMP levels
Tokuo et al., FEBS Lett 2001 : DDAH increased PKA phosphorylation of native neurofibromin in a dose dependent manner, especially affecting the phosphorylation of CSRD
Herring et al., Cell Signal 2001 (MAP Kinase Signaling System) : Therefore, the objective of the present studies was to determine the role of cAMP and protein kinase A (PKA) in the CBN mediated inhibition of IL-2, CREB , and NF-kappaB in PMA/Io activated thymocytes ... Therefore, the objective of the present studies was to determine the role of cAMP and protein kinase A (PKA) in the CBN mediated inhibition of IL-2 , CREB, and NF-kappaB in PMA/Io activated thymocytes ... Therefore, the objective of the present studies was to determine the role of cAMP and protein kinase A (PKA) in the CBN mediated inhibition of IL-2, CREB, and NF-kappaB in PMA/Io activated thymocytes
Won et al., Brain Res Mol Brain Res 2001 (Glioma) : This study has demonstrated the mechanism of protein kinase A (PKA) dependent inhibition of astrocytic nitric oxide production and inducible NO synthase mRNA expression induced by lipopolysaccharide ... The dose dependent inhibition of NO production and iNOS mRNA expression by compound SB203580 ( p38 inhibitor ) suggests the participation of p38 in PKA dependent inhibition of LPS induced NO production and iNOS mRNA expression
Ciullo et al., Oncogene 2001 : Moreover, PKA phosphorylates immunoprecipitated p85 and PKA phosphorylation of cell extracts significantly stimulates the formation of the complex PI3K-Ras ... We suggest that PKA phosphorylates p85 and stabilizes the complex p110-p85 , enhancing the interaction PI3K and p21 Ras
Button et al., J Gen Physiol 2001 (Ion Channel Gating) : Activation of CFTR by PKA stimulation appears to be mediated by a complex interaction between several consensus phosphorylation sites in the regulatory domain ( R domain ) ... Here, we show that although endogenous phosphorylation by PKC is required for the effect of PKA on CFTR , stimulation of PKC by itself has only a minor effect on human CFTR ... In contrast, CFTR from the amphibians Necturus maculosus and Xenopus laevis ( XCFTR ) can be activated to similar degrees by stimulation of either PKA or PKC
Yamagishi et al., J Biol Chem 2001 : Leptin activated PKA , and the effects of leptin were inhibited by the cAMP antagonist Rp-cAMPS
Sziklai et al., Acta Otolaryngol 2001 (Synaptic Transmission) : Simultaneous application of ACh and H-89, a selective protein kinase A (PKA) inhibitor , did not antagonize the ACh response
Osna et al., Int Immunopharmacol 2001 : PKA played a role in the inhibition of IFN gamma by histamine when the cells were activated via TCR, and the PKA inhibitors Rp-cAMPS ( 10 ( -5 ) M ) and H8 ( 10 ( -5 ) M ) reversed the inhibitory effects of histamine on IFN gamma production ... However, when the cells were stimulated with ionomycin, the PKA inhibitors did not affect histamine mediated suppression of IFN gamma production
Cao et al., J Biol Chem 2001 : The involvement of PKA in beta ( 3 ) AR-dependent p38 MAPK activation was confirmed by the ability of the PKA inhibitors H89 ( 20 microm ) and ( R ( p ) ) -cAMP-S ( 1 mm ) to block phosphorylation of p38 MAPK ... The involvement of PKA in beta ( 3 ) AR-dependent p38 MAPK activation was confirmed by the ability of the PKA inhibitors H89 ( 20 microm ) and ( R ( p ) ) -cAMP-S ( 1 mm ) to block phosphorylation of p38 MAPK ... Moreover, the PKA dependent transactivation of the UCP1 promoter, as well as its sensitivity to SB202190, was fully reproduced by a 220-nucleotide enhancer element from the UCP1 gene
Yao et al., Biochemistry 2001 : A second-order loss of Ca-ATPase activation by PKA is observed as a function of the fractional contribution of FITC-PLB, indicating that PKA dependent activation of two PLB molecules within a quaternary complex containing the Ca-ATPase is necessary for activation of the Ca-ATPase ... We suggest that the requirement for activation of two PLB molecules by PKA represents a physiological mechanism to ensure that activation of the Ca-ATPase following beta-adrenergic stimulation in the heart only occurs above a threshold level of PKA activation
Shen et al., Diabetes Res Clin Pract 2001 : On the other hand, it did not affect PKA stimulated insulin release under either conditions, but in the case of 100 microM, glucose augmented the insulin secretion in the presence of glyceraldehyde and db-cAMP concentration-dependently
Miggin et al., Biochim Biophys Acta 2001 : Both U46619 and 8-epiPGF ( 2alpha ) induced ERK activations were inhibited by the protein kinase ( PK ) C, PKA and phosphoinositide 3-kinase inhibitors GF109203X, H-89 and wortmannin, respectively, but were unaffected by pertussis toxin
Billiard et al., J Biol Chem 2001 : IGF-I production by rat osteoblasts is stimulated by activation of cAMP dependent protein kinase (PKA) ... Inhibition of active PKA with targeted nuclear expression of PKA inhibitor had no effect on the subcellular location of C/EBPdelta but prevented hormone induced IGF-I gene activation, while cytoplasmic PKA inhibitor additionally caused the removal of C/EBPdelta from the nucleus ... Inhibition of active PKA with targeted nuclear expression of PKA inhibitor had no effect on the subcellular location of C/EBPdelta but prevented hormone induced IGF-I gene activation, while cytoplasmic PKA inhibitor additionally caused the removal of C/EBPdelta from the nucleus ... Cytoplasmic PKA mediates nuclear localization of C/EBPdelta under basal conditions, and nuclear PKA stimulates its transcriptional activity upon hormone treatment
Jessop et al., J Bone Miner Res 2001 : Activation of ERK-1 and the phosphorylation of ER-alpha , by both estrogen and strain, were prevented by the MAP kinase kinase ( MEK ) inhibitor U0126 and the protein kinase A (PKA) inhibitor ( PKI ) ... Activation of ERK-1 and the phosphorylation of ER-alpha, by both estrogen and strain, were prevented by the MAP kinase kinase ( MEK ) inhibitor U0126 and the protein kinase A (PKA) inhibitor ( PKI )
Lopez et al., Endocrine 2001 : Cotransfection studies carried out in Kin 8 cells, a Y1 cell line with a mutation that prevents cAMP activation of PKA, revealed that a functional PKA is required for cAMP induction of SCP2 gene transcription
Westmark et al., Brain Res Mol Brain Res 2001 (MAP Kinase Signaling System) : However the protein kinase C ( PKC ), protein kinase A (PKA) or PLC pathways did not mediate these changes in APP mRNA levels
Pombo et al., Horm Res 2001 : It is well established that binding of GHRH to its receptor leads to activation of protein kinase A (PKA)
Shashkin et al., J Basic Clin Physiol Pharmacol 2001 (Insulin Resistance) : In turn, insulin stimulated cPIP synthase activity was inversely related to both the insulin stimulated fractional activity of PKA ( r=0.89, p < 0.02 ) and the insulin stimulated total PKA activity : r=0.94, p < 0.005
Li et al., Mol Cell Biol 2001 : Phosphorylation at CIITA serines 834 and 1050 accounts for the inhibitory effects of PKA on CIITA-driven class II MHC transcription
Chakravarty et al., J Biol Chem 2001 : The more than 10-fold induction of PKA stimulated PEPCK-C gene transcription caused by the co-activator CBP, was also blocked by SREBP-1c ... In addition, dnSREBP-1c reversed the strong negative effect of E1A and NF1 on PKA stimulated transcription from the PEPCK-C gene promoter
Lopez et al., Endocrine 2001 : The involvement of cyclic adenosine monophosphate cAMP dependent protein kinase A (PKA) in the regulation of the steroidogenic acute regulatory protein ( StAR ) and the high-density lipoprotein receptor ( HDL-R ) genes by steroidogenic factor-1 (SF-1) and cAMP were examined
Zhou et al., Peptides 2001 : PACAP activates PKA , PKC and Ca ( 2+ ) signaling cascades in rat neuroepithelial cells
Kiriyama et al., Endocrinology 2001 : Furthermore, calcitonin induced IL-6 production was completely suppressed by H-89 ( PKA inhibitor ) or GF109203X ( PKC inhibitor ), indicating that the activation of both PKA and PKC is necessary for calcitonin induced IL-6 production
Yang et al., FASEB J 2001 (Ion Channel Gating) : In hippocampal neurons, InsP6 stimulates adenylyl cyclase ( AC ) without influencing cAMP phosphodiesterases, resulting in activation of protein kinase A (PKA) and thereby selective enhancement of voltage gated L-type Ca2+ channel activity
Tartaglia et al., J Biol Chem 2001 : However, BDNF did not activate PKA , and application of a PKA activator did not mimic the BDNF effect
Sauer et al., Cytokine 2001 (Leukemia, Megakaryoblastic, Acute) : We investigated the effect of TPO on activation of cAMP dependent protein kinase (PKA) and its involvement in MAPK signalling in human megakaryoblastic leukaemia CMK cells ... TPO enhanced cAMP and induced activation of PKA in CMK cells
Wyatt et al., Am J Physiol Lung Cell Mol Physiol 2001 : We hypothesized that the loss of EtOH stimulated CBF was a result of downregulation of PKA activity
D'Angiolella et al., Curr Biol 2001 : Blocking PKA , instead, stabilizes cdk2 activity and inhibits cyclin B degradation and cdk1 inactivation
Jiang et al., Proc Natl Acad Sci U S A 2001 : Inhibition of either PKA or MEK1/2 blocked ERK1/2 activation by glucagon ... We conclude that glucagon induced MEK1/2 and ERK1/2 activation is mediated by PKA and that an increase in intracellular calcium concentration is required for maximal ERK activation ... We conclude that glucagon induced MEK1/2 and ERK1/2 activation is mediated by PKA and that an increase in intracellular calcium concentration is required for maximal ERK activation
Danaher et al., J Neurovirol 2001 (Encephalitis, Herpes Simplex) : Taken together, PACAP induced HSV-1 activation from QIF-PC12 cells involves the PKA and possibly cAMP independent pathways
Monroy et al., J Biol Chem 2001 : We show that transcription of a PEPCK chloramphenicol acetyltransferase (CAT) reporter gene activated by protein kinase A (PKA) is enhanced 7-fold by SRCAP ... In addition, SRCAP in the presence of PKA enhances the ability of the Gal-CREB chimera to activate transcription of a Gal-CAT reporter gene that contains only binding sites for Gal4
Szászi et al., J Biol Chem 2001 : Inhibition of NHE3 by cytochalasin D does not require PKA , because the inhibitory effect is preserved in a mutant NHE3 that is not phosphorylated by PKA and in cells pretreated with the PKA inhibitor H89
Weinman et al., J Biol Chem 2001 : Prior studies have indicated a requirement for the PDZ domain containing protein, Na ( + ) /H ( + ) Exchanger Regulatory Factor ( NHERF ), for protein kinase A (PKA) mediated inhibition of the renal basolateral Na(+)-HCO(3)(-) co-transporter ( NBC )
Reimer et al., Endocrinology 2001 : Activators of PKA and PKC were able to increase GLP-1 secretion in NCI-H716 cells
Connor et al., Mol Cell Biol 2001 : In contrast, PKA phosphorylation was required for PP1 binding and inhibition by the N-terminal I-1 ( 1-80 ) fragment
Singh et al., Diabetes 2001 : Pharmacologic inhibition of protein kinase C ( PKC ) and protein kinase A (PKA) , which are involved in hexosamine mediated matrix production, blocked the CREB phosphorylation and fibronectin synthesis seen in HG and GlcN conditions
Kudoh et al., Brain Res 2001 (Magnesium Deficiency...) : In this paper, protein kinase C ( PKC ) and calcium/calmodulin dependent protein kinase type II ( CaMKII ) but not protein kinase A (PKA) were reported to contribute to the potentiation mechanism during a step between the activation of the N-methyl-D-aspartate receptors by the Mg ( 2+ ) -free treatment and the secretion of the protein factor ( s )
Um et al., Oncogene 2001 : Furthermore, PKA inhibitor suppressed markedly the constitutive and drug induced activities of NF-kappaB and Ku in MDR cells and subsequently potentiated the cytotoxic activity of anticancer drugs
Nakamura et al., J Am Soc Nephrol 2001 : However, inhibition of MAPK ( p42/p44 ) and TNF-alpha promoter activity was partially prevented by the cAMP-protein kinase (PKA) inhibitors, H-89 ( 5 x 10 ( -6 ) M ) and KT5720 ( 10 ( -5 ) M ), whereas the suppression of p38 MAPK or NF-kappa B ( p50 ) was not blocked by these inhibitors ... However, inhibition of MAPK ( p42/p44 ) and TNF-alpha promoter activity was partially prevented by the cAMP-protein kinase (PKA) inhibitors, H-89 ( 5 x 10 ( -6 ) M ) and KT5720 ( 10 ( -5 ) M ), whereas the suppression of p38 MAPK or NF-kappa B ( p50 ) was not blocked by these inhibitors ... It is likely that cAMP-PKA and MAPK ( p42/p44, p38 ) may play a critical role in the regulation of the Stx-2 induced TNF-alpha transcription via beta(2)-adrenoceptor activation
Baert et al., J Biol Chem 2002 : We show here that protein kinase A (PKA) increases the transcriptional activity of human ERM and human ETV1 , through a Ser residue situated at the edge of the ETS DNA binding domain
Alexander et al., Invest Ophthalmol Vis Sci 2001 : Agents that modulate protein kinase A (PKA) or inhibit phosphatidylinositol 3-kinase (PI3K) had minimal effects on trabecular MMP or TIMP induction by TNFalpha, whereas several agents that modulate PKC activity were effective
Rodríguez-Esteban et al., Development 2001 : Both the adhesion protein N-cadherin and PKI ( an endogenous protein kinase A inhibitor ) are localized to the right side of the node and may contribute to restrict Nodal activation by Wnt signaling and PKA to the left side of the node ... Both the adhesion protein N-cadherin and PKI ( an endogenous protein kinase A inhibitor ) are localized to the right side of the node and may contribute to restrict Nodal activation by Wnt signaling and PKA to the left side of the node
Niu et al., Curr Biol 2001 : Interaction of heterotrimeric G13 protein with an A-kinase anchoring protein 110 (AKAP110) mediates cAMP independent PKA activation ... Finally, AKAP110 significantly potentiated G alpha 13-induced activation of PKA ... Finally, AKAP110 significantly potentiated G alpha 13-induced activation of PKA ... Finally, AKAP110 significantly potentiated G alpha 13-induced activation of PKA
Cochrane et al., J Interferon Cytokine Res 2001 : In contrast, inhibitors of PKA or NOS, but not catalase, prevented FSK induced suppression of IFN-gamma production
Deeble et al., Mol Cell Biol 2001 (MAP Kinase Signaling System...) : Convergent IL-6 and PKA signaling also resulted in potentiated mitogen activated protein kinase ( MAPK ) activation without affecting the level of signal transducer and activator of transcription or PKA activation observed with these agents alone
Graack et al., Biochem J 2001 : Expressing Dmel/GFAT1 in yeast we showed that Dmel/GFAT1 activity is controlled by UDP-N-acetylglucosamine and PKA in the yeast total protein extract system
Antos et al., Circ Res 2001 (Cardiomyopathy, Dilated...) : As seen in human heart failure, these abnormalities correlated with PKA mediated hyperphosphorylation of the cardiac ryanodine receptor/Ca ( 2+ ) -release channel, which enhances Ca ( 2+ ) release from the sarcoplasmic reticulum, and phospholamban , which regulates the sarcoplasmic reticulum Ca ( 2+ ) -ATPase
Leicht et al., Cardiovasc Res 2001 : The increase in collagen I, collagen III, MMP-2, TIMP-2 , and colligin evoked by serum depletion was also diminished by PKA inhibition ... The increase in collagen I, collagen III, MMP-2 , TIMP-2, and colligin evoked by serum depletion was also diminished by PKA inhibition ... The increase in collagen I, collagen III, MMP-2, TIMP-2, and colligin evoked by serum depletion was also diminished by PKA inhibition ... The increase in collagen I, collagen III, MMP-2 , TIMP-2, and colligin evoked by serum depletion was also diminished by PKA inhibition
Herron et al., Circ Res 2001 : Consistent with previous studies, PKA increased phosphorylation levels of myosin binding protein C and troponin I, and reduced Ca ( 2+ ) sensitivity of force
Higuchi et al., Mol Cell Biol 2002 : Here we show that Rst2p , a zinc finger protein that can bind to the upstream region of ste11 and fbp1 via the STREP motif, mediates the activity of PKA to transcription of these genes ... Rst2p was mainly cytoplasmic under high PKA activity but was concentrated in the nucleus when this activity was lowered, suggesting that PKA might regulate ste11 and fbp1 negatively by excluding Rst2p from the nucleus
Dudas et al., Am J Physiol Regul Integr Comp Physiol 2002 : The effect of parathyroid hormone (PTH) and activation of protein kinase C ( PKC ) and protein kinase A (PKA) on transepithelial P ( i ) transport was examined in monolayers of chick proximal tubule cells in primary culture ( PTCs ) ... These data indicate that PTH inhibition of P ( i ) reabsorption in this in vitro system is mediated by PKC and PKA
Aesøy et al., Endocrinology 2002 : These findings were confirmed by Western blot analysis, showing that stimulation of PKA increased SF-1 protein levels ... The PKA- induced expression of SF-1 protein was not accompanied by an increase in SF-1 mRNA levels
Souza et al., J Biol Chem 2002 : Activation of cyclic AMP dependent protein kinase (PKA) results in the phosphorylation of Peri A and hormone-sensitive lipase (HSL) , the predominant lipase in adipocytes, with concurrent stimulation of adipocyte lipolysis
Tsukada et al., Neurosci Lett 2002 : To examine whether the PKA activation is due to inhibition of phosphodiesterase III (PDE III) by cyclic GMP, we applied PDE III-specific inhibitors and found that the inhibitions activated PKA
Lieb et al., J Physiol 2002 : After increasing human CBF in a PKA dependent manner to a stable plateau with forskolin ( 10 microM ), ATP caused only a transient, calcium coupled CBF increase
Mei et al., J Biol Chem 2002 : Activation of Epac leads to a phosphatidylinositol 3-kinase dependent PKB activation, while stimulation of PKA inhibits PKB activity ... The opposing effects of Epac and PKA on PKB activation provide a potential mechanism for the cell type-specific differential effects of cAMP
Schmitt et al., Mol Cell 2002 : We demonstrate here that PKA 's activation of Rap1 was mediated by the Rap1 guanine nucleotide exchange factor C3G, the adaptor Crk-L, the scaffold protein Cbl, and the tyrosine kinase Src ... PKA activated Src both in vitro and in vivo by phosphorylating Src on serine 17 within its amino terminus
Swarthout et al., Gene 2002 : However, while PKA is linked to many of the changes in gene expression directed by PTH, PKA activation has been shown to inhibit mitogen activated protein kinase ( MAPK ) and proliferation of osteoblasts
Nesher et al., Diabetes 2002 (Diabetes Mellitus, Type 2) : In the isolated rat pancreas, accelerated calcium entry or activation of protein kinase (PK)-A or PKC result in no insulin response in the absence of fuel metabolism ... We suggest that three families of PKs ( calmodulin dependent PK [ CaMK ], PKA , and PKC ) function as distal amplifiers for stimulus-secretion coupling signals originating from fuel metabolism, as well as from incretins acting through membrane receptors, adenylate cyclase, and phospholipase C
Thibault et al., J Leukoc Biol 2002 : The activator of protein kinase A (PKA) , Sp-cAMP inhibited fMLP induced PLD activation and translocation of Arf and RhoA to membranes ... The activator of protein kinase A (PKA) , Sp-cAMP inhibited fMLP induced PLD activation and translocation of Arf and RhoA to membranes ... The activator of protein kinase A (PKA) , Sp-cAMP inhibited fMLP induced PLD activation and translocation of Arf and RhoA to membranes
Suwa et al., J Biol Chem 2002 : The dominant negative mutant of vinexin beta inhibited the anchorage independent ERK2 activation induced by the PKA inhibitor
Murthy et al., Am J Physiol Cell Physiol 2002 : PKA dependent activation of PDE3A and PDE4 and inhibition of adenylyl cyclase V/VI in smooth muscle ... PKA dependent activation of PDE3A and PDE4 and inhibition of adenylyl cyclase V/VI in smooth muscle ... PKA dependent activation of PDE3A and PDE4 and inhibition of adenylyl cyclase V/VI in smooth muscle
Dahan et al., Pflugers Arch 2001 (Cystic Fibrosis) : PKA is an important activator, and its stimulation of CFTR is enhanced by PKC via mechanisms which are not fully understood
Laliberté et al., FEBS Lett 2002 : In vitro PKA phosphorylation mediated human PDE4A4 activation
Imamur et al., Diabetes Nutr Metab 2001 : Our results show that gliclazide stimulated 2-deoxy- [ 3H ] -D-glucose ( 2DG ) uptake, 24 h after treatment, in a dose dependent manner, and it also increased GLUT 1 protein synthesis and mRNA expression ; 2DG uptake and GLUT 1 protein synthesis induced by gliclazide were completely blocked by protein kinase A (PKA) inhibitors ( H89 and rp-cAMP ), and gliclazide increased the intracellular cAMP levels 3 to 24 hr after the treatment
Huang et al., Mol Pharmacol 2002 : Moreover, the activation of beta-adrenergic receptors by isoproterenol mimics forskolin to elicit a PKA dependent inhibition of CB(1) receptor function
Kim et al., Biochem Biophys Res Commun 2002 (Neuroblastoma) : Interestingly, KT5720, a specific inhibitor of PKA , markedly suppressed the phosphorylation of MAPK by N/OFQ in SH-SY5Y cells
Li et al., Circ Res 2002 (Calcium Signaling) : However, clear cellular data on PKA dependent modulation of cardiac RyRs is limited because of difficulty in distinguishing between PKA effects on RyR , phospholamban (PLB), and Ca2+ current ... However, clear cellular data on PKA dependent modulation of cardiac RyRs is limited because of difficulty in distinguishing between PKA effects on RyR, phospholamban (PLB), and Ca2+ current ... PKA activation also increased Ca2+ spark amplitude, duration, and width in WT, but not in PLB-KO or PLB-DM. RyR phosphorylation was confirmed by measurements of 32P incorporation on immunoprecipitated RyR ... In intact resting myocytes, PKA activation increased CaSpF 2.8-fold in WT, but not in PLB-KO , confirming results in permeabilized myocytes
Leemhuis et al., J Pharmacol Exp Ther 2002 : Activated protein kinase A (PKA) inhibits RhoA and induces extensions
Whitehouse et al., J Endocrinol 2002 : We have now investigated the responses to PKA activation and inhibition of PP1/2A and used PTP inhibitors to examine the relationship between the morphological changes and enhanced steroid production
Amano et al., Jpn J Pharmacol 2001 : These results suggested that AC/PKA dependent induction of VEGF certainly enhanced angiogenesis and that pharmacological tools for controlling this signaling pathway may be able to facilitate the management of conditions involving angiogenesis
Constantinescu et al., J Biol Chem 2002 : We propose that type II PKA is translocated and activated in the nucleus and induces CREB phosphorylation that is necessary but not sufficient for gene transcription
Crépieux et al., Arch Biochem Biophys 2002 : In comparison, in the absence of anchorage, ERK1, 2 were still activated by epidermal growth factor , in a PKA dependent manner ... In comparison, in the absence of anchorage, ERK1 , 2 were still activated by epidermal growth factor, in a PKA dependent manner ... In comparison, in the absence of anchorage, ERK1 , 2 were still activated by epidermal growth factor, in a PKA dependent manner
Christian et al., J Biol Chem 2002 : Reporter gene studies showed that FKHR potently enhances PKA dependent activation of the tissue-specific decidual prolactin ( dPRL ) promoter, a major differentiation marker in human ES cells
Dajas-Bailador et al., J Neurochem 2002 (MAP Kinase Signaling System...) : In contrast, two structurally different inhibitors of PKA ( KT 5720 and H-89 ) completely prevented the nicotine dependent increase in ERK1/2 activity
Dwivedi et al., J Pharmacol Exp Ther 2002 : These results, thus, suggest that HAL and CLOZ differentially regulate PKA catalytic and regulatory activities and the expression of selective catalytic and regulatory subunit isoforms of PKA, which may be associated with their mechanisms of action
Shiraha et al., Mol Cell Biol 2002 : EGF induced calpain activation was inhibited by cAMP activation of protein kinase A (PKA) , as the PKA inhibitors H-89 and Rp-8Br-cAMPS abrogated cAMP inhibition of both motility and calpain activation
Lau et al., Br J Haematol 2002 (MAP Kinase Signaling System) : Activation of protein kinase A (PKA) , however, lowered PAI-1 secretion induced by uPA and tPA, as a result of an inhibition of the PKC pathway and inhibition of Raf, Mek and MAPK phosphorylations
Børud et al., Mol Endocrinol 2002 : PKA was shown to stimulate SF-1 transcriptional activity, and coexpression of p/CIP together with the PKA catalytic subunit stimulated SF-1 mediated transactivation even further
Godwin et al., Bone 2002 (Second Messenger Systems) : In addition, exposure to pertussis toxin or exogenous activation of protein kinase A (PKA) inhibited PLC-gamma1 tyrosine phosphorylation in response to Ca ( e )
Spurzem et al., Am J Physiol Lung Cell Mol Physiol 2002 : Augmentation of PKA activity reduced the levels of active Rho and the formation of focal adhesions
Adachi et al., J Biol Chem 2002 : We have evaluated whether post-translational modification of Arix regulates PKA mediated DBH gene transcription ... The results collectively suggest that dephosphorylation of Arix is a necessary event to fully activate PKA mediated DBH transcription
Gevrey et al., J Biol Chem 2002 : This was associated with a nuclear translocation of the PKA catalytic subunit and with a PKA dependent phosphorylation of the CRE binding protein ( CREB ) at Ser ( 133 )
Edwards et al., Alcohol Clin Exp Res 2002 : We measured the protein kinase A (PKA) mediated phosphorylation of DARPP-32 and the NR1 subunit of the NMDA receptor ... SKF82958 alone significantly increased PKA mediated phosphorylation of both DARPP-32 and NR1 ... However, when D1 receptors were blocked, bromocriptine reduced the PKA mediated phosphorylation of both DARPP-32 and NR1
Dhillon et al., Mol Cell Biol 2002 : The cyclic AMP ( cAMP ) -dependent protein kinase (PKA) can inhibit Raf-1 by direct phosphorylation ... cAMP increased Raf-1 serine 259 phosphorylation in a PKA dependent manner with kinetics that correlated with ERK deactivation ... Using RafS259 mutants we also demonstrate that Raf-1 is the sole target for PKA inhibition of ERK and ERK induced gene expression, and that Raf-1 inhibition is mediated mainly through serine 259 phosphorylation ... Using RafS259 mutants we also demonstrate that Raf-1 is the sole target for PKA inhibition of ERK and ERK induced gene expression, and that Raf-1 inhibition is mediated mainly through serine 259 phosphorylation
Yu et al., Brain Res Mol Brain Res 2002 (Synaptic Transmission) : Our data indicate that the protein inhibitor of nNOS (PIN) binds to protein kinase A inhibitors ( PKIs ), which suggests that PKIs, together with PIN , might mediate the phosphorylation of nNOS by PKA ... Our data indicate that the protein inhibitor of nNOS (PIN) binds to protein kinase A inhibitors ( PKIs ), which suggests that PKIs, together with PIN, might mediate the phosphorylation of nNOS by PKA
Tsingotjidou et al., Bone 2002 : PTH induces IL-6 through PKA activation, whereas fluprostenol induces IL-6 through PKC activation ... PTH induces IL-6 through PKA activation, whereas fluprostenol induces IL-6 through PKC activation
Salazar et al., Mol Biol Cell 2002 : Furthermore, PKA inhibition may contribute to ligand induced EGFR endocytosis because epidermal growth factor inhibited 26 % of PKA basal activity
Kawaguchi et al., J Neurosci 2002 : We confirmed that inhibition of Ca ( 2+ ) /calmodulin dependent protein kinase II ( CaMKII ) or PKA suppresses RP
Sorensen et al., Mol Pharmacol 2002 (Second Messenger Systems) : In the present study, we report that in addition to protein kinase C ( PKC ), cAMP dependent protein kinase (PKA) and cGMP dependent protein kinase ( PKG ) can inhibit calmodulin (CaM) interactions with the carboxyl-terminal tail of mGluR7
Williams et al., J Immunol 2002 : Disruption of the AKAP-PKA interaction caused high levels of IL-2 , IL-4, IL-5, and IFN-gamma production in the absence of stimulation via CD3epsilon and CD28 molecules ... Disruption of the AKAP-PKA interaction caused high levels of IL-2, IL-4 , IL-5, and IFN-gamma production in the absence of stimulation via CD3epsilon and CD28 molecules ... Disruption of the AKAP-PKA interaction caused high levels of IL-2, IL-4, IL-5 , and IFN-gamma production in the absence of stimulation via CD3epsilon and CD28 molecules ... Disruption of the AKAP-PKA interaction caused high levels of IL-2, IL-4, IL-5, and IFN-gamma production in the absence of stimulation via CD3epsilon and CD28 molecules
Feldman et al., J Biol Chem 2002 : For the beta ( 2 ) AR in HEK-293 cells, where PKA activity is required for ERK activation, expression of GsCT caused a net inhibition of ERK activation
Jones et al., J Leukoc Biol 2002 : In this work, we examined the role of PKA in PMN alphaMbeta2 integrin activation
Ang et al., J Neurochem 2002 (Second Messenger Systems) : Together, these data show reciprocal regulation of PDE1C and PDE4D by PKA , which represents a novel scheme for plasticity in intracellular signalling ... Together, these data show reciprocal regulation of PDE1C and PDE4D by PKA , which represents a novel scheme for plasticity in intracellular signalling
Gallo et al., J Neurosci 2002 : We found that BDNF causes transient activation of protein kinase A (PKA) during the first 5 min of treatment ... The BDNF induced activation of PKA was required for BDNF mediated stabilization of growth-cone actin filaments against depolymerization by cytochalasin D
Deckelbaum et al., J Cell Sci 2002 : Ihh enhances differentiation of CFK-2 chondrocytic cells and antagonizes PTHrP mediated activation of PKA ... In addition, overexpression of a constitutively active form of the PTHrP receptor ( PTHR1 H223R ) inhibited Ihh mediated differentiation, implicating activation of protein kinase A (PKA) by PTHR1 as a probable mediator of the antagonistic effects of PTHrP ... Conversely, overexpression of Ihh/N-Ihh or exogenous treatment with N-Shh led to dampening of PTHrP mediated activation of PKA
Sugino et al., Hum Reprod 2002 : To study whether the increase in Mn-SOD by db-cAMP or E ( 2 ) + MPA was mediated by cAMP dependent protein kinase A (PKA) , ESC were incubated with protein kinase inhibitor ( PKI ) ( 10 microg/ml ), an inhibitor of PKA, in the presence of db-cAMP or E ( 2 ) + MPA
Onoue et al., FEBS Lett 2002 : These results suggest that PACAP27 attenuates PrP ( 106-126 ) -induced delayed neurotoxicity in PC12 cells by activating both PKA and MAP kinases mediated by PAC1 receptor
Tamura et al., J Clin Endocrinol Metab 2002 : COX-2 mRNA, protein, and PGE ( 2 ) levels in IL-1beta treated ESC were decreased by a PKA inhibitor, a nuclear factor ( NF-kappaB ) inhibitor, and an ERK1/2 inhibitor, but not by a p38 MAPK inhibitor or a PKC inhibitor, suggesting the possible involvement of PKA , NF-kappaB, and/or the ERK1/2 signaling pathway ( s ) in IL-1beta mediated COX-2 gene induction in ESC
Leão-Ferreira et al., Arch Virol 2002 : Furthermore, Ado induced PKA activation modulates negatively an unidentified step of the virus replicative cycle
Jo et al., Circ Res 2002 : In the heart, stimulation of beta2-adrenoceptor (beta2-AR) , a prototypical GPCR, activates a tightly localized protein kinase A (PKA) signaling, which regulates substrates at cell surface membranes, bypassing cytosolic target proteins ( eg, phospholamban )
Cheng et al., J Biol Chem 2002 (MAP Kinase Signaling System) : Results from cAMP-response element binding protein ( CREB ) phosphorylation at serine 133, trans reporting assays, and overexpression of two dominant negative CREB mutants revealed that A ( 2A ) -R stimulation led to activation of CREB in a PKA dependent manner and subsequently reversed the damage of NGF evoked neurite outgrowth by PD98059 or dnMAPK
Yamamoto et al., Brain Res Mol Brain Res 2002 (Brain Edema) : In this study, we investigated the effects of a protein kinase A (PKA) activator on the expression of AQP4 , 5 and 9 in cultured rat astrocytes ... The dbcAMP induced effects on AQP5 and AQP9 mRNAs were inhibited by PKA inhibitors ... The dbcAMP induced effects on AQP5 and AQP9 mRNAs were inhibited by PKA inhibitors
Li et al., Cell Res 2002 : In the presence of AFP ( 20 mg/L ), the content of cAMP and activities of PKA were significantly elevated
Choi et al., Ann N Y Acad Sci 2002 : Here we review the evidence that ( 1 ) the synergistic interactions of cholera toxin and other pharmacological agents on mast cell secretion are attributable to the synergistic activation of PLD via PKA , CaM kinase II, and PKC and ( 2 ) both PLD1 and PLD2 participate in this process
Amano et al., Hum Cell 2002 : These results suggested that COX-2 and AC/PKA dependent induction of VEGF certainly enhanced angiogenesis, and that pharmacological tools for controlling this signaling pathway may be able to facilitate the management of conditions involving angiogenesis
Salvador et al., Endocrinology 2002 : The PKA inhibitor H89 as well as the myristoylated PKA inhibitor peptide PKI strongly inhibited hCG stimulated p42/44 MAPK phosphorylation, whereas the PKC inhibitor GF109203X had no effect on p42/44 MAPK phosphorylation ... Taken together, these results indicate that, under primary culture conditions where physiological levels of signaling proteins are present, hCG signals to activate MEK, p42/44 MAPK, CREB , and histone H3 in a predominantly PKA dependent and PKC independent manner ... Taken together, these results indicate that, under primary culture conditions where physiological levels of signaling proteins are present, hCG signals to activate MEK, p42/44 MAPK, CREB, and histone H3 in a predominantly PKA dependent and PKC independent manner ... Taken together, these results indicate that, under primary culture conditions where physiological levels of signaling proteins are present, hCG signals to activate MEK, p42/44 MAPK, CREB, and histone H3 in a predominantly PKA dependent and PKC independent manner ... Taken together, these results indicate that, under primary culture conditions where physiological levels of signaling proteins are present, hCG signals to activate MEK, p42/44 MAPK , CREB, and histone H3 in a predominantly PKA dependent and PKC independent manner ... Taken together, these results indicate that, under primary culture conditions where physiological levels of signaling proteins are present, hCG signals to activate MEK, p42/44 MAPK , CREB, and histone H3 in a predominantly PKA dependent and PKC independent manner
Duca et al., FEBS Lett 2002 : The elastin peptides mediated induction of pro-collagenase-1 production by human fibroblasts involves activation of MEK/ERK pathway via PKA- and PI(3)K dependent signaling
Forti et al., Biochemistry 2002 (Adrenal Cortex Neoplasms) : ACTH promotion of p27 ( Kip1 ) induction in mouse Y1 adrenocortical tumor cells is dependent on both PKA activation and Akt/PKB inactivation
Cai et al., J Biol Chem 2002 : We concluded that serotonin, by activating 5-HT(1A) receptors, suppress glutamatergic signaling through the inhibition of CaMKII, which is achieved by the inhibition of PKA and ensuing activation of PP1
Harada et al., J Cell Biol 2002 : Therefore, MAP2 is an anchoring protein of PKA in dendrites, whose loss leads to reduced amount of dendritic and total PKA and reduced activation of CREB
Chiu et al., J Biol Chem 2002 : Here we demonstrate that protein kinase A (PKA) dependent phosphorylation of Nopp140 , together with C/EBPbeta, induces agp gene expression synergistically ... Here we demonstrate that protein kinase A (PKA) dependent phosphorylation of Nopp140, together with C/EBPbeta , induces agp gene expression synergistically ... Results from biochemical and functional characterizations of Nopp140 mutants defective in PKA phosphorylation sites suggest that PKA dependent Nopp140 phosphorylation is important for its role in agp gene activation
Uchida et al., J Biol Chem 2002 : These results suggest that AP-2 and Sp1 regulate basal promoter activity, and Sp1 is also involved in PKA mediated expression of the rat SPT gene in concert with the transcriptional coactivator CBP
Takizawa et al., J Allergy Clin Immunol 2002 : PKC and PKA negatively regulate PAF induced CD11b upregulation and cellular adhesion but promote eosinophil effector functions, such as superoxide anion generation and degranulation
Trümper et al., J Endocrinol 2002 (MAP Kinase Signaling System) : These interactions included : ( i ) a central role of tyrosine phosphorylation for stimulation of PKA/CREB, MAPK and PI3-kinase/PKB, ( ii ) inhibition of PKA/CREB by the MAPK pathway at the level of MAPK kinase-1 or downstream, ( iii ) activation of MAPK signaling by PI3-kinase and PKA at the level of extracellular-signal regulated kinase 1/2 or upstream, and ( iv ) activation of PKB by MAPK and PKA signaling at the level of PKB or upstream ... These interactions included : ( i ) a central role of tyrosine phosphorylation for stimulation of PKA/CREB, MAPK and PI3-kinase/PKB, ( ii ) inhibition of PKA/CREB by the MAPK pathway at the level of MAPK kinase-1 or downstream, ( iii ) activation of MAPK signaling by PI3-kinase and PKA at the level of extracellular-signal regulated kinase 1/2 or upstream, and ( iv ) activation of PKB by MAPK and PKA signaling at the level of PKB or upstream ... These interactions included : ( i ) a central role of tyrosine phosphorylation for stimulation of PKA/CREB, MAPK and PI3-kinase/PKB, ( ii ) inhibition of PKA/CREB by the MAPK pathway at the level of MAPK kinase-1 or downstream, ( iii ) activation of MAPK signaling by PI3-kinase and PKA at the level of extracellular-signal regulated kinase 1/2 or upstream, and ( iv ) activation of PKB by MAPK and PKA signaling at the level of PKB or upstream ... These interactions included : ( i ) a central role of tyrosine phosphorylation for stimulation of PKA/CREB, MAPK and PI3-kinase/PKB, ( ii ) inhibition of PKA/CREB by the MAPK pathway at the level of MAPK kinase-1 or downstream, ( iii ) activation of MAPK signaling by PI3-kinase and PKA at the level of extracellular-signal regulated kinase 1/2 or upstream, and ( iv ) activation of PKB by MAPK and PKA signaling at the level of PKB or upstream
Brown et al., Biol Reprod 2002 : By compartmentalizing PKA, AKAP140 and/or other AKAPs could spatially regulate PKA activity during oocyte development
Laurich et al., Endocrinology 2002 (Leydig Cell Tumor) : Protein kinase A (PKA) inhibition with H-89 blocked Cyp17 mRNA induction, suggesting that MIS interferes with the PKA signal transduction pathway
Bhave et al., Neuron 2002 (Pain) : In this study, we demonstrate that PKA reduces VR1 desensitization and directly phosphorylates VR1 ... Electrophysiological analysis of phosphorylation site mutants clearly pinpoints Ser116 as the residue responsible for PKA dependent modulation of VR1
See et al., J Biol Chem 2002 : Furthermore, we demonstrate that phosphorylation of ABCA1 is mediated by protein kinase A (PKA) or a PKA-like kinase in vivo ... These studies demonstrate a novel serine ( Ser-2054 ) on the ABCA1 protein crucial for PKA phosphorylation and for regulation of ABCA1 transporter activity
Huening et al., Mol Endocrinol 2002 : Our data indicate that the mechanism of the inhibitory effect of ICER involves an inhibition of PKA induced VDR transcription, and this inhibition may be mediated in part by binding of ICER to a cAMP response element-like sequence in the VDR promoter
Lesage et al., Hepatology 2002 : Insulin induced activation of PKC alpha, which decreased secretin stimulated cAMP and PKA activity
Sirotkin et al., Reprod Nutr Dev 2002 : The expression of the PKA regulatory subunit was increased by EGF ( 51 % compared with 29 % in the control, p < 0.05 ), but not by IGF-I or IGF-II ( 30 and 29 % ) ... The expression of the PKA regulatory subunit was increased by EGF ( 51 % compared with 29 % in the control, p < 0.05 ), but not by IGF-I or IGF-II ( 30 and 29 % ) ... The expression of the PKA regulatory subunit was increased by EGF ( 51 % compared with 29 % in the control, p < 0.05 ), but not by IGF-I or IGF-II ( 30 and 29 % )
Zhou et al., Biochem Biophys Res Commun 2002 : CREB DNA binding activation by a 50-Hz magnetic field in HL60 cells is dependent on extra- and intracellular Ca ( 2+ ) but not PKA , PKC, ERK, or p38 MAPK ... Furthermore, We found that the MF-induced increase of CREB DNA binding in HL60 cells was dependent on both extracellular and intracellular Ca ( 2+ ) but not PKA , PKC, ERK, or p38 MAPK by using various pathway inhibitors
Nishi et al., Br J Pharmacol 2002 : UTP ( 100 microM ) did not activate PKA
Ranganathan et al., J Biol Chem 2002 : To demonstrate the importance of the AKAPs, inhibition of PKA-AKAP binding with a peptide competitor ( HT31 ) prevented epinephrine mediated inhibition of LPL translation
Schmitt et al., J Biol Chem 2002 (MAP Kinase Signaling System) : In contrast, the activation by isoproterenol of Ras requires Gbetagamma subunits, is independent of PKA , and results in the phosphoinositol 3-kinase dependent activation of AKT
Takahashi et al., Eur J Biochem 2002 (MAP Kinase Signaling System) : PKA activators affected neither IkappaBalpha phosphorylation, IkappaBetaalpha degradation, nor the NF-kappaBeta/DNA binding ... Overexpression of coactivators, CBP ( CREB binding protein ) and p300, failed to reverse the PKA mediated inhibition of p65 transactivation
Liu et al., Endocrinology 2002 : IGF-I increased CREB phosphorylation at a concentration as low as 10 ng/ml, and this effect was inhibited by H89, a PKA inhibitor, and PD98059, a MAPK kinase inhibitor
Tremblay et al., Endocrinology 2002 : We now report that GATA-4 is phosphorylated in MA-10 cells in response to cAMP and in heterologous CV-1 cells, GATA-4 transcriptional activity is stimulated by PKA ... We now report that GATA-4 is phosphorylated in MA-10 cells in response to cAMP and in heterologous CV-1 cells, GATA-4 transcriptional activity is stimulated by PKA ... Moreover, we show that GATA-4 and C/EBPbeta directly interact in vitro and in vivo and synergistically activate the StAR promoter in CV-1 cells exclusively in the presence of PKA
Park et al., Mol Cell Endocrinol 2002 : This study aimed to investigate whether the cAMP/PKA or the PKC system is involved in STAT3 activation in response to TSH ... Increased intracellular cAMP activated STAT3 but inhibition of PKA did not affect STAT3 activation
Sheridan et al., J Biol Chem 2002 : Here we show that overexpression of PKA causes phosphorylation and cytoplasmic accumulation of NF-ATc1 in direct opposition to calcineurin by phosphorylating Ser-245, Ser-269, and Ser-294 in the conserved serine-proline repeat domain, and that mutation of these serines blocks the effect of PKA
Said et al., Pflugers Arch 2002 : The relative contribution of Thr ( 17 ) phosphorylation to the isoproterenol induced phosphorylation of PLB and relaxation thus increased with the level of beta-adrenoceptor stimulation and the consequent increase in PKA activity
Misra et al., J Biol Chem 2002 : The functional role of PKA and PKC phosphorylation sites in PBP remains to be elucidated
Kotak et al., J Neurobiol 2002 : In this study, we asked whether the calcium dependency of inhibitory depression was associated with the activation of calcium/calmodulin dependent protein kinase II ( CaMKII ), protein kinase C ( PKC ), and/or cAMP dependent protein kinase A (PKA)
Zeng et al., American journal of physiology. Renal physiology 2002 (Second Messenger Systems) : We conclude that, in human mesangial cells, CaMK and PKA can both contribute to cell cycle entry, and, although induction of c-fos by CaMK requires active PKA , neither pathway antagonizes or synergizes c-fos induction by the other
Chen et al., Am J Physiol Cell Physiol 2002 (Osteosarcoma) : Consistent with the role for cAMP/PKA signaling in this response, PTH induces transient cAMP elevation, PKA activation, and cAMP-responsive element binding protein ( CREB ) phosphorylation
Boo et al., Am J Physiol Heart Circ Physiol 2002 : These results suggest that shear stress stimulates eNOS by two different mechanisms : 1 ) PKA- and PI3K dependent and 2 ) PKA dependent but PI3K independent pathways
D'Souza et al., Am J Physiol Cell Physiol 2003 : The increased levels of p53 and p21 observed in eNOS transfected cells were reduced in the presence of the PKA inhibitor ... The increased levels of p53 and p21 observed in eNOS transfected cells were reduced in the presence of the PKA inhibitor ... These data suggest that p21 and p53 play a role in the inhibition of proliferation in eNOS transfected cells and that levels of these two proteins are regulated by PKA ... These data suggest that p21 and p53 play a role in the inhibition of proliferation in eNOS transfected cells and that levels of these two proteins are regulated by PKA
Han et al., Vis Neurosci 2002 : In these neurons, protein kinase A (PKA) up-regulated the NMDA evoked [Ca2+ ] i increase
Brandon et al., Dev Growth Differ 2002 : Although some early development genes appear to require both Galpha3 and PKA for expression, the differing response of cAR1 expression and the inhibitory effect of Galpha3 on PKA activity suggest that Galpha3 and PKA are members of interacting pathways controlling gene expression early in development
Fietta et al., Cytokine 2002 (MAP Kinase Signaling System...) : In addition, activation of PKA and the phosphoinoside 3-kinase (PI-3k)/p70 ribosomal S6 kinase cascade was required to have maximal MCP-1 but not IL-8 production
Chang et al., Cell Signal 2003 (Brain Neoplasms) : Conclusively, we present that both the disturbances of mitochondrial calcium homeostasis and reactive oxygen intermediates are essential for rapid transactivation of grp78, and this pathway is separate from protein kinase A (PKA) dependent CREB activation or p38 mitogen activated protein kinase ( p38 ( MAPK ) ) -dependent ATF-2 activation and signalling ... Conclusively, we present that both the disturbances of mitochondrial calcium homeostasis and reactive oxygen intermediates are essential for rapid transactivation of grp78, and this pathway is separate from protein kinase A (PKA) dependent CREB activation or p38 mitogen activated protein kinase ( p38 ( MAPK ) ) -dependent ATF-2 activation and signalling
Wu et al., Oncogene 2002 (Cell Transformation, Neoplastic...) : Here we show that c-MYC induces the activity of Protein Kinase A (PKA) , a key effector of cAMP mediated signal transduction, by inducing the transcription of the gene encoding the PKA catalytic subunit beta (PKA-Cbeta)
Inglefield et al., Neuroscience 2002 (Synaptic Transmission) : By contrast, inhibition of protein kinase A (PKA) with 100 microM PKA inhibitor peptide, PKI, blocked A1254 induced CREB phosphorylation
Ni et al., J Biol Chem 2003 : Although proglucagon transcription and GLP-1 synthesis were shown to be activated by forskolin and other protein kinase A (PKA) activators, deleting or mutating the cAMP-response element ( CRE ) only moderately attenuates the proglucagon gene promoter in response to PKA activation
Groussin et al., Am J Hum Genet 2002 (Adenocarcinoma...) : The mutant protein was present in patients ' leukocytes and tumors, and in vitro studies indicated that the mutant PRKAR1A activated cAMP dependent protein kinase A (PKA) signaling at the nuclear level
Affaitati et al., J Biol Chem 2003 : Here we show that conditional expression of AKAP121 in PC12 cells selectively enhances cAMP.PKA signaling to mitochondria ... AKAP121 induction stimulates PKA dependent phosphorylation of the proapoptotic protein BAD at Ser ( 155 ), inhibits release of cytochrome c from mitochondria, and protects cells from apoptosis
Woo et al., Eur J Neurosci 2002 (Synaptic Transmission) : Our data show that genetic inhibition of PKA impairs L-LTP by reducing PKA mediated suppression of PP1/2A
Haidar et al., J Lipid Res 2002 (Tangier Disease) : In contrast, H-89 PKA inhibitor reduced cellular cholesterol efflux by 70 % in a dose dependent manner and inhibited almost completely ABCA1 phosphorylation
Stenfors et al., J Neural Transm 2002 : The results indicate that activation of tryptophan hydroxylase by reducing the tone from terminal 5-HT(1B) receptors involves PKA whereas the depolarisation induced activation of tryptophan hydroxylase involves CaM kinase II
Ikewaki et al., Microbiol Immunol 2002 : Taken together, these findings indicate that BU49 induces CREB phosphorylation mainly mediated by PKA , which finally results in the induction of homotypic cell aggregation and the enhancement of IL-8 production ... Furthermore, these findings also indicate that the enhancement of IL-8 production from the U937 cells induced by BU49 partially depends on CREB phosphorylation mainly mediated by PKA
Ferraris et al., Proc Natl Acad Sci U S A 2002 : Conclusions : ( i ) PKA plays an important role in TonEBP/OREBP activation of tonicity dependent gene expression ; ( ii ) PKA activation of TonEBP/OREBP appears to be cAMP independent ; and ( iii ) amino acids 872-1271 are sufficient for tonicity dependent transactivation of TonEBP/OREBP
Yano et al., Mol Cell Biochem 2002 : PKA and PKCzeta activators of forskolin and okadaic acid increased catalase activity and 110 kDa expression in NIH3T3 cells up to 2.4-fold and suppressed the cell growth, showing an inverse correlation of the indices ( r : -0.9286, r2 : 0.8622, n : 18, p < 0.0001 )
Masmoudi et al., FASEB J 2003 : Pituitary adenylate cyclase activating polypeptide ( PACAP ) stimulates endozepine release from cultured rat astrocytes via a PKA dependent mechanism
Montiel et al., J Neurosci Res 2003 (Calcium Signaling) : Interestingly, although protein kinase A (PKA) inhibition by H-89 did not affect the high K ( + ) or DMPP induced SNAP-25 expression, basal protein levels were significantly modified upon activation or inhibition of this pathway
Poderoso et al., Endocr Res 2002 : Taken together, these results indicate that PTP activity participates in StAR protein induction and led us to attribute to the PKA mediated PTP activation in steroidogenic systems a functional role, as mediator of StAR protein induction
Lund et al., Endocr Res 2002 : Thus, whereas p/CIP stimulated SF-1 dependent transcription is further potentiated by PKA , we show that activation of PKA leads to selective downregulation of TIF2 protein and a subsequent repression of TIF2 coactivator function
Kim et al., Brain Res Mol Brain Res 2002 : Taken together, these pharmacological and genetic transfection studies provide compelling evidence for the role of PKA activation on neuronal differentiation in HiB5 hippocampal progenitor cells
Tang et al., J Neurosci 2003 (Calcium Signaling) : We discovered that PKA activates and PP1alpha inhibits the activity of recombinant InsP3R1 reconstituted into planar lipid bilayers
Hollinger et al., Biochemistry 2003 : Because of this proximity, we focused on the possible effects of PKA phosphorylation on the GDI activity of RGS14 ... Because of this proximity, we focused on the possible effects of PKA phosphorylation on the GDI activity of RGS14
Konhilas et al., J Physiol 2003 : To clarify the role of PKA dependent phosphorylation of TnI and MyBP-C on LDA in the heart, we examined LDA in skinned myocytes from a non-transgenic ( NTG ) and a transgenic murine model in which the native cardiac isoform ( cTnI ) was completely replaced by the slow skeletal isoform of TnI ( ssTnI-TG ) lacking the phosphorylation sites for PKA, while retaining PKA sites on MyBP-C
Lee et al., Experimental biology and medicine (Maywood, N.J.) 2003 : Leptin has been reported to activate phosphatidylinositol 3-kinase (PI 3-K) and subsequently phosphodiesterase ( PDE ) to impair protein kinase A (PKA) induced insulin secretion from cultured islets of neonatal rats ... We determined if PKA induced insulin secretion was also hyperresponsive in islets from Lep ( ob ) /Lep ( ob ) mice, and if leptin impaired this pathway in islets from these mice
Li et al., Blood 2003 : Here we show that, in human platelets, phosphorylation of VASP at Ser239 induced by either cGMP analogs or nitric oxide ( NO ) donor glyco-SNAP1 is inhibited by PKA inhibitors KT5720, PKI, Rp-Br-cAMPS, and H89, but not by PKG inhibitors KT5823 or Rp-pCPT-cGMPS ... Unlike human platelets, cGMP analog induced phosphorylation of VASP in mouse platelets is inhibited by both PKG and PKA inhibitors ... Thus, PKA plays a predominant role in the cGMP induced phosphorylation of VASP and platelet inhibition in human platelets
Bengoechea-Alonso et al., Nitric Oxide 2003 : These observations are consistent with the inhibition caused by W-7, NMLA, H-89, and cyclosporin A on NF-kappaB induction by these agonists, indicating the involvement of PKA and PP2B in the regulation of NF-kappaB in human monocytes
Qiao et al., Am J Physiol Lung Cell Mol Physiol 2003 : PKA inhibits RhoA activation : a protection mechanism against endothelial barrier dysfunction ... In conclusion, the results provide evidence that PKA inhibited RhoA activation in endothelial cells, supporting a signaling mechanism of protection against vascular endothelial barrier dysfunction
Chappe et al., J Physiol 2003 : Phosphorylation of protein kinase C sites in NBD1 and the R domain control CFTR channel activation by PKA
Piiper et al., Biochem Biophys Res Commun 2003 : Chemical inhibition of PKA suppressed forskolin induced EGFR tyrosine phosphorylation and ERK1/2 activation in PC12 cells ... Chemical inhibition of PKA suppressed forskolin induced EGFR tyrosine phosphorylation and ERK1/2 activation in PC12 cells ... Together, our data indicate that activation of PKA mediates the effects of cAMP on the EGFR and ERK1/2 ... Together, our data indicate that activation of PKA mediates the effects of cAMP on the EGFR and ERK1/2 ... While PKA may directly phosphorylate the EGFR on serine residues, PKA induced tyrosine phosphorylation of the EGFR occurs by an indirect mechanism
Dyer et al., Biochem Biophys Res Commun 2003 (Ion Channel Gating) : The effects of PKA on the type I InsP(3) receptor using single channel electrophysiological studies were also investigated
Anderson et al., Br J Pharmacol 2003 : Western blot of Caco-2 cell protein demonstrates expression of the NHE regulatory factor NHERF1 ( expression of which is thought to be required for PKA mediated inhibition of NHE3 )
Yaqub et al., Biochem J 2003 : In the present study, we investigate the mechanism for the protein kinase A (PKA) mediated activation of C-terminal Src kinase (Csk) ... This suggests that an overall structural domain organization and interaction between the kinase and SH3 domains are important for the activity of Csk and its regulation by PKA
Anand et al., Biol Reprod 2003 (MAP Kinase Signaling System) : Gonadotropin stimulated VEGF production was mediated by cAMP dependent protein kinase A (PKA) , as evidenced by the effect of hCG being mimicked by 8Br-cAMP and being abolished in the presence of a PKA-specific inhibitor, H-89
Abrahamsen et al., J Biol Chem 2003 : We propose a novel mechanism whereby G protein coupled receptors inhibit Src signaling by activation of Csk in a cAMP-PKA dependent manner
Than et al., Eur J Biochem 2003 (Carcinoma, Squamous Cell...) : Cytostatic agents induced apoptosis and PP17b synthesis in HeLa cells, which was significantly inhibited by protein kinase C ( PKC ) inhibitor, indicating the involvement of NF-kappa B and AP-1 transcription factors in this process, while protein kinase A (PKA) inhibitor had only a modest inhibitory effect
Cho et al., Clin Cancer Res 2003 (Colonic Neoplasms...) : The results showed that PKA-I activation by Gem231 and topoisomerase I inhibition by HCPT are responsible at the molecular level for the synergistic effects of tumor cell apoptosis and growth inhibition ... The results showed that PKA-I activation by Gem231 and topoisomerase I inhibition by HCPT are responsible at the molecular level for the synergistic effects of tumor cell apoptosis and growth inhibition
Galdiero et al., J Infect 2003 : The inhibitors of PTK, PKA and PKC activation used, were : 3,4-dihydroxybenzylidene-malononitrile ( tyrphostin 23 ), inhibitor of epidermal growth factor (EGF) receptor tyrosine kinase activity ; dihychloride ( H-89 ), a selective inhibitor of PKA which is useful to discriminate between the effects of PKC and PKA ; diacylglycerol kinase inhibitor II ( R59949 ), which is useful for elucidating roles of PKC ; calphostin C, a specific inhibitor of PKC
Wong et al., Metabolism 2003 : To examine the role of PKA in mediating an increase in AVP V2 mRNA expression, we incubated IMCD with 10 ( -7 ) and 10 ( -11 ) M of angiotensin II in the presence of a specific protein kinase A (PKA) inhibitor, Rp diasteroisomer of adenosine 3'-5'-cylic monophosphothionate ( Rp-cAMPS )
Kannan et al., Cell Biol Int 2003 : E-NTPase/E-NTPDase : a potential regulatory role in E-kinase/PKA mediated CD36 activation
Pilon et al., Biochim Biophys Acta 2003 : We have previously shown that, in the human adrenocortical carcinoma cell line, NCI-H295R, SR-BI and LDL receptor expression and steroidogenesis are coordinately regulated by activators of protein kinase A (PKA) leading to glucocorticoid synthesis ... We have previously shown that, in the human adrenocortical carcinoma cell line, NCI-H295R, SR-BI and LDL receptor expression and steroidogenesis are coordinately regulated by activators of protein kinase A (PKA) leading to glucocorticoid synthesis
Tremblay et al., J Biol Chem 2003 : We now show that the rapid phosphorylation of GATA-4 induced by cAMP in vivo can be blocked by a PKA-specific inhibitor but not by mitogen activated protein kinase inhibitors, indicating that GATA-4 is predominantly phosphorylated by PKA in response to cAMP in gonadal cells ... Finally, PKA dependent phosphorylation of GATA-4 also led to enhanced recruitment of the CREB binding protein coactivator
Campos et al., Mol Cell Neurosci 2003 (Calcium Signaling...) : Promoter analyses have revealed that the DRE site, a silencer element present in the prodynorphin promoter, is involved in PKA dependent prodynorphin derepression
Higuchi et al., EMBO J 2003 : Activation of cAMP-PKA is required for translocation of p75 ( NTR ) to lipid rafts, and for biochemical and biological activities of p75 ( NTR ), such as inactivation of Rho and the neurite outgrowth
Tortora et al., Clin Cancer Res 2003 (Neovascularization, Pathologic) : COX-2 expression can be induced by EGFR activation and is regulated by cAMP and PKA ... COX-2 expression can be induced by EGFR activation and is regulated by cAMP and PKA
Takeuchi et al., J Neurochem 2003 (Neuroblastoma) : Stimulation with forskolin and overexpression of constitutively active PKA suppressed NF-kappaB activity, likely due to D1R stimulation
Horgan et al., Exp Cell Res 2003 : However, PKA activity was also not necessary for the early translocation of Erk into the nucleus by NGF or Ras, but it was able to induce a small influx of Erk that could be measured with GFP-Erk2
Ruehr et al., J Biol Chem 2003 : Therefore, localization of PKA by mAKAP at RyR1 increases both PKA dependent RyR phosphorylation as well as efflux of Ca2+ through the RyR
Fujita et al., Prostaglandins Leukot Essent Fatty Acids 2003 : Investigation of intracellular signaling verified that low-density PGE ( 2 ) suppressed PKA activity in undifferentiated cells, suggesting that PGE ( 2 ) acts on the osteoclastic cell lineage to facilitate cell differentiation by suppressing PKA in the presence of RANKL
Yao et al., Med Sci Monit 2003 : The specific PKA inhibitor reduces MCP-1 gene expression and translation, as well as moderately reducing CSF-1 and PTHrP-R expression ... The specific PKA inhibitor reduces MCP-1 gene expression and translation, as well as moderately reducing CSF-1 and PTHrP-R expression ... The specific PKA inhibitor reduces MCP-1 gene expression and translation, as well as moderately reducing CSF-1 and PTHrP-R expression
Kunzmann et al., FASEB J 2003 : Furthermore, the histamine effect on TGF-beta1 responsiveness was cAMP/PKA dependent
Pacheco et al., Brain Res Mol Brain Res 2003 : Activation of PKA by forskolin ( 10 microM ), dibutyryl-cAMP ( 1 mM ), or Sp-8-Br-cAMP ( 1 mM ) enhanced phosphorylation nearly threefold ; the inactive isomer, Rp-8-Br-cAMP ( 1 mM ) had no effect
Pirih et al., Biochem Biophys Res Commun 2003 : PTH activates cAMP-protein kinase A (PKA) , protein kinase C ( PKC ), and calcium signaling
Robinson-White et al., Hum Mol Genet 2003 : We investigated whether the PKA mediated inhibitory effect on ERK1/2 is affected in CNC cells that carry germline PRKAR1A mutations
Yoon et al., J Biol Chem 2003 : Taken together, our results indicate that stimulation of PKA by alpha-MSH causes inhibition of LPS induced activation of p38 kinase and NF kappa B to block TNF-alpha production
Bae et al., Biochem Biophys Res Commun 2003 : This acute effect of BK on both increases was blocked only by treatment of protein kinase A inhibitor H-89, but not by the inhibitors of calmodulin dependent kinase II and protein kinase B, suggesting that the rapid increase in NO production by BK is mediated by the PKA dependent phosphorylation of eNOS-Ser ( 1179 )
Nakamura et al., Biochem Pharmacol 2003 : However, inhibition of Stx2 mediated caspase activity by terbutaline was partially blocked by cAMP-PKA inhibitors
Gutzkow et al., Cell Signal 2003 : The purpose of the present study was to understand the mechanism by which activated protein kinase A (PKA) leads to down-regulation of cyclin D3 in lymphocytes ... Taken together, it appears that activation of PKA in Jurkat cells reduces the expression of cyclin D3 at the level of translational elongation by increasing the phosphorylation of eEF2 and thereby inhibiting its activity
Zhou et al., J Biol Chem 2003 : In addition, expression of S66A mutant ezrin in cultured parietal cells attenuates the dilation of apical vacuolar membrane associated with stimulation by histamine, indicating that PKA mediated phosphorylation of ezrin is necessary for acid secretion ... These data suggest that PKA mediated phosphorylation of ezrin plays an important role in mediating the remodeling of the apical membrane cytoskeleton associated with acid secretion in parietal cells
Xie et al., Proc Natl Acad Sci U S A 2003 : PKA directly phosphorylates PTB on conserved Ser-16, and PKA activation in PC12 cells induces Ser-16 phosphorylation
Piontek et al., J Biol Chem 2003 : In contrast, binding of PKCbetaII and PKA were independent of Ca2+ and PKC inhibition
Zhu et al., J Biol Chem 2003 : As a consequence, apoE also increased protein kinase A (PKA) activity in smooth muscle cells
Vinciguerra et al., Mol Biol Cell 2003 : The [ Na+ ] i-dependent increase in Na, K-ATPase cell-surface expression was prevented by PKA inhibitors H89 and PKI
Hansen et al., Neurosci Lett 2003 : The results demonstrate that PKA and ERK play a key role in KCl- and forskolin induced neuronal differentiation by integration of signals from both pathways
Lehnart et al., Curr Top Med Chem 2003 (Ion Channel Gating) : In addition, the FKBP-RyR interaction is regulated by PKA phosphorylation ... In addition, the FKBP-RyR interaction is regulated by PKA phosphorylation
Dabizzi et al., Cancer Res 2003 (Endometrial Neoplasms...) : Moreover the hrLH induced increase in Hec1A invasiveness relied upon a PKA dependent functional activation of beta ( 1 ) integrin receptors, as well as the subsequent induction of matrix metalloproteinase-2 secretion in its active form
Lee et al., Biochem Pharmacol 2003 : Involvement of PKA and Sp1 in the induction of p27 ( Kip1 ) by tamoxifen ... Taken together, our results suggest that Tam transcriptionally activates p27 ( Kip1 ) expression via the Sp1 consensus sites in the p27 ( Kip1 ) promoter and PKA is involved in this process
Pomerance et al., Mol Endocrinol 2003 : The use of pharmacological inhibitors indicated that cAMP induced CHOP expression was dependent on protein kinase A (PKA) , mammalian target of rapamycin pathway, and reactive oxygen species
Aizawa et al., Circ Res 2003 : The effects of SNAP and CNP on NF-kappaB are mediated by cAMP dependent protein kinase (PKA) but not cGMP dependent protein kinase ( PKG ) based on the findings that the selective PKA inhibitor, PKI, abolished the effects of SNAP and CNP on NF-kappaB, whereas the PKG inhibitor Rp-8-Br-PET-cGMP had no effect ... The effects of SNAP and CNP on NF-kappaB are mediated by cAMP dependent protein kinase (PKA) but not cGMP dependent protein kinase ( PKG ) based on the findings that the selective PKA inhibitor, PKI, abolished the effects of SNAP and CNP on NF-kappaB, whereas the PKG inhibitor Rp-8-Br-PET-cGMP had no effect ... The effects of SNAP and CNP on NF-kappaB are mediated by cAMP dependent protein kinase (PKA) but not cGMP dependent protein kinase ( PKG ) based on the findings that the selective PKA inhibitor, PKI, abolished the effects of SNAP and CNP on NF-kappaB, whereas the PKG inhibitor Rp-8-Br-PET-cGMP had no effect ... Activation of PKA by SNAP or cAMP elevating agents not only inhibited TNF-alpha induced NF-kappaB dependent reporter gene expression but also reduced endogenous NF-kappaB dependent adhesion molecule and chemokine expression
Thomson et al., Exp Dermatol 2003 : We tested the effects of PTHrP on protein synthesis, protein kinase A (PKA) and protein kinase C ( PKC ) activities as well as tyrosine phosphorylation in rat vibrissa dermal papilla and capsular fibroblast cells
Wyatt et al., Am J Pathol 2003 : These data support that EtOH stimulated increases in CBF require the dual activation of both PKA ( via cAMP ) and PKG ( via NO )
Wagner et al., J Biol Chem 2003 : In cells expressing the neuronal, S2+ splice variant of the InsP3R-1, Ca2+ release was markedly enhanced when either PKA or PKG was activated ... Activation of PKA also resulted in potentiated Ca2+ release in cells expressing the non-neuronal, S2- splice variant of the InsP3R-1
Cherian et al., J Biol Chem 2003 : The adenylate cyclase activators, forskolin and 8-bromo-cAMP, enhanced intercellular connectivity, the number of functional gap junctions, and Cx43 protein expression, whereas the PKA inhibitor, H89, inhibited the stimulatory effect of PGE2 on gap junctions
Tremblay et al., J Steroid Biochem Mol Biol 2003 : GATA-4 phosphorylation is mediated by PKA
Takemori et al., J Steroid Biochem Mol Biol 2003 : ACTH induced the SIK 's nuclear to cytosolic translocation in a PKA dependent manner
Martins et al., Biochemistry 2003 (Second Messenger Systems) : Our results indicate that in vitro, the interaction between HA95 and LAP2beta is influenced by a PKA mediated phosphorylation of HA95 rather than by CDK1- or PKC mediated phosphorylation of LAP2beta ... Our results indicate that in vitro, the interaction between HA95 and LAP2beta is influenced by a PKA mediated phosphorylation of HA95 rather than by CDK1- or PKC mediated phosphorylation of LAP2beta
Franceschi et al., Connect Tissue Res 2003 : Interactions with Runx2 are complex involving both binding of distinct components such as AP-1 factors and Smads to separate sites on DNA, direct interactions between Runx2 and AP-1/Smad factors and MAPK or PKA dependent Runx2 phosphorylation
Masci et al., J Leukoc Biol 2003 : Of note, the ligation of CXCR4 by stromal cell derived factor-1alpha but not the engagement of CD4 by monoclonal antibody stimulates the PKA activity and induces a long lasting hyporesponsivity state in naive CD4+ lymphocytes ... Of note, the ligation of CXCR4 by stromal cell derived factor-1alpha but not the engagement of CD4 by monoclonal antibody stimulates the PKA activity and induces a long lasting hyporesponsivity state in naive CD4+ lymphocytes
Alava et al., Biochem J 1992 : These results suggest that PKA mediated phosphorylation of PLC may regulate TCR/CD3 induced InsPL hydrolysis
Chen et al., Endocrinology 1992 : TNF alpha increased cAMP and also increased relative PKA and PKC activity in 1-40 min. Phorbol myristate acetate ( PMA ), an activator of PKC, increased [ 3H ] thymidine incorporation and DNA content
Zhang et al., J Immunol 1992 : In contrast, induction of IL-8 by RA was inhibited by both 1- ( 5-isoquinoline sulfonamide and N- ( 2-gamidinoethyl ) -5-isoquinoline sulfonamide, suggesting the participation of PKA in the induction of IL-8 by RA ... However, activation of PKA by addition of cAMP analogues was not sufficient to induce IL-8 expression
de Groot et al., Oncogene 1992 : Here we show that Jun/AP-1 is activated by the cAMP dependent protein kinase A (PKA) ... Here we show that Jun/AP-1 is activated by the cAMP dependent protein kinase A (PKA)
Janulis et al., J Clin Invest 1992 : PTH can activate both the adenylate cyclase/protein kinase A (PKA) and the plasma membrane phospholipase C/protein kinase C ( PKC ) pathways
Arnold et al., Symp Soc Exp Biol 1992 (Cell Transformation, Neoplastic) : Constitutive expression of the catalytic subunit of PKA completely suppresses transactivation of the myogenin promoter by Myf-5 or MyoD1 suggesting that cAMP may act via phosphorylation by PKA
Andrews et al., Cancer Res 1992 (Prostatic Neoplasms) : In this study, we examine the effects of protein kinase A (PKA) and protein kinase C ( PKC ) on the androgen regulation of PSA in a human adenocarcinoma cell line, LNCaP
Yang et al., J Membr Biol 2003 (Calcium Signaling) : EGF induces CCE through its stimulation of Erkl/2 activity, whereas PKA stimulation suppresses these effects of EGF
Kageyama et al., J Cardiovasc Pharmacol 2003 : Both SQ22536, an adenylate cyclase inhibitor, and Rp-8-Br-cAMPS, a protein kinase A (PKA) inhibitor , were found to attenuate the urocortin II-induced vasodilation
Schulte et al., Exp Cell Res 2003 : Inhibition of cAMP dependent protein kinase (PKA) using H89 ( N- [ 2- ( ( p-bromocinnamyl ) amino ) ethyl ] -5-isoquinolinesulfonamide ) ) blocked phosphorylation of the cAMP response element binding protein ( CREB ) and p38 , but did not decrease NECA induced ERK1/2 phosphorylation ... Inhibition of cAMP dependent protein kinase (PKA) using H89 ( N- [ 2- ( ( p-bromocinnamyl ) amino ) ethyl ] -5-isoquinolinesulfonamide ) ) blocked phosphorylation of the cAMP response element binding protein ( CREB ) and p38, but did not decrease NECA induced ERK1/2 phosphorylation ... p38 activation by NECA was instead independent of PI3K but required cAMP and PKA
Sowa et al., J Biol Chem 2003 : Protein kinase A (PKA) activator as well as protein kinase C ( PKC ) activators increased Smad3 protein level, and both PKA and PKC inhibitors antagonized PTH induced Smad3, indicating that PTH promotes the production of Smad3 through both PKA and PKC pathways
Yang et al., J Immunol 2003 (Glioma) : However, KT5720, a selective PKA inhibitor, reduced neither anti-Fas triggered nor glioma associated IL-10 expression
Lonart et al., Cell 2003 : Our findings suggest that PKA mediated phosphorylation of the active zone protein RIM1alpha at a single N-terminal site induces presynaptic LTP
Ishizaki et al., Exp Cell Res 2003 : These findings indicate that both PKA induced phosphorylation of claudin-5 immunoprecipitates and cAMP dependent but PKA independent induction of claudin-5 expression could be involved in promotion of tight-junction function in endothelial cells
Boo et al., Free Radic Biol Med 2003 : We examined whether phosphorylation of S635 of eNOS induced by PKA stimulates NO production in a calcium independent manner
Yang et al., J Neurophysiol 2004 : Considerable evidence has shown that calcium/calmodulin dependent protein kinase II ( CaMKII ), protein kinase A (PKA) , and protein kinase C ( PKC ) are important for LTP in hippocampus
Baldassa et al., Brain Res Mol Brain Res 2003 (MAP Kinase Signaling System) : Moreover, protein kinase A (PKA) activity is required for depolarization induced Rap1 activation and full Erk stimulation, but is not involved in that of Ras ... Moreover, protein kinase A (PKA) activity is required for depolarization induced Rap1 activation and full Erk stimulation, but is not involved in that of Ras ... This PKA dependent Rap1 activation does not require Src family kinases, but, in contrast to Ras, is sensitive to genistein, indicating the involvement of a tyrosine kinase dependent mechanism
Sudo et al., Platelets 2003 : The PKA/PKG inhibitor, H-89, inhibited VASP phosphorylation by cilostazol
Hayashi et al., Am J Physiol Cell Physiol 2004 : Using molecular and electrophysiological techniques, we examined whether cytosolic ATP dependent regulation of native SK4/IK1-like channel activity would involve endogenous cAMP dependent protein kinase (PKA) in rat submandibular acinar ( RSA ) cells
Cluck et al., Mol Cell Endocrinol 2003 : Amylin gene expression mediated by cAMP/PKA and transcription factors HNF-1 and NFY ... These results indicate that amylin is positively regulated by cAMP and PKA through the transcription factors HNF-1 and NFY
Tiwari et al., Blood 2004 (Leukemia, Lymphocytic, Chronic, B-Cell) : The EPAC-specific cAMP analog 8CPT-2Me-cAMP ( 8- ( 4-chloro-phenylthio ) -2'-O-methyladenosine-3',5'-cAMP ) activates Rap1 in B-CLL cells, but, unlike rolipram/forskolin or 8-Bromo-cAMP, it does not induce PKA activation, as judged by phosphorylation of the transcription factor cAMP-response element binding protein ( CREB ) ... The EPAC-specific cAMP analog 8CPT-2Me-cAMP ( 8- ( 4-chloro-phenylthio ) -2'-O-methyladenosine-3',5'-cAMP ) activates Rap1 in B-CLL cells, but, unlike rolipram/forskolin or 8-Bromo-cAMP, it does not induce PKA activation, as judged by phosphorylation of the transcription factor cAMP-response element binding protein ( CREB )
Zhao et al., Leukemia 2004 (Leukemia, Promyelocytic, Acute...) : Moreover, we found that cAMP dependent protein kinase (PKA) activity was quickly upregulated in parallel in ATRA treated NB4 cells, and the phosphorylation of RARalpha by PKA could increase its transactivation effect
Firulli et al., Mol Cell 2003 : PKA and PKC phosphorylate HAND proteins in vivo, and only B56delta containing PP2A complexes reduce levels of HAND1 phosphorylation
Chen et al., Neuroscience 2003 : A cAMP dependent protein kinase (PKA) selective inhibitor and an adenylate cyclase inhibitor blocked both PKA activation and the subsequent filopodia formation
Lorenz et al., J Biol Chem 2004 : Our results indicate that EGCG induced endothelium dependent vasodilation is primarily based on rapid activation of eNOS by a phosphatidylinositol 3-kinase-, PKA- , and Akt dependent increase in eNOS activity, independently of an altered eNOS protein content
Leong et al., Mol Endocrinol 2004 (Adenocarcinoma...) : DIM stimulated MAPK activity and induced phosphorylation of the endogenous PKA target, cAMP response element binding protein ( CREB ), in a PKA dependent manner ... These results demonstrate that DIM is a mechanistically novel activator of the ER that requires PKA dependent phosphorylation of CREB
Tojima et al., J Neurosci Res 2003 : These results suggest strongly that PKA simultaneously plays two different roles in neuronal differentiation : inhibition of neuritogenesis and stimulation of synaptogenesis, via CREB mediated gene expression
Ke et al., Biochem Biophys Res Commun 2003 (Body Weight) : Overexpression of leptin in transgenic mice leads to decreased basal lipolysis, PKA activity, and perilipin levels
Schiller et al., Oncogene 2003 : Inhibition of protein kinase A (PKA) , the main downstream effector of cAMP, with H-89, suppressed cAMP dependent repression of SMAD-driven gene expression ... cAMP elevating agents did not inhibit nuclear translocation and DNA binding of SMAD3/4 complexes, but abolished the interactions of SMAD3 with the transcription coactivators CREB binding protein (CBP) and p300 in a PKA dependent manner
Vijayaragavan et al., J Neurophysiol 2004 : PKA induced increases ( Nav1.8 ) and decreases ( Nav1.7 ) in peak currents were not associated with shifts in voltage dependent activation or inactivation
Tang et al., J Biol Chem 2004 (Fibrosarcoma) : Conversely, PKA but not JAK2 inhibition significantly reduced the intrinsic cholesterol translocase activity of ABCA1
Kalma et al., Endocrinology 2004 : We show herein that LH inhibits Cx43 expression by reducing its rate of translation and that this effect is mediated by both PKA and MAPK
Vogel et al., J Biol Chem 2004 : Cotransfection experiments with CREB and PKA expression plasmids further supported our conclusions that the TCDD dependent effect on C/EBPbeta transcription is mediated via PKA dependent CREB activation
Mao et al., Exp Cell Res 2004 : Whereas PKA from cells treated with forskolin activated stress kinase p38, PKA from cells treated with sorbitol did not activate p38 , although the enzyme is activated in both cases as analysed in vitro using a specific peptide target ... Whereas PKA from cells treated with forskolin activated stress kinase p38 , PKA from cells treated with sorbitol did not activate p38, although the enzyme is activated in both cases as analysed in vitro using a specific peptide target ... Finally, in CHO cells overexpressing wild-type MLTKalpha, no activation of AMPK of PKA could be demonstrated, indicating that the activated kinase cascades are not involved in a cross-talk process
Reinehr et al., Gastroenterology 2004 : Inhibition of bile salt induced apoptosis by cAMP involves both PKA dependent Ser/Thr phosphorylation of the CD95 and inhibition of EGF-R activation, which results in an inhibition of CD95 tyrosine phosphorylation, CD95 membrane targeting, and DISC formation ... Inhibition of bile salt induced apoptosis by cAMP involves both PKA dependent Ser/Thr phosphorylation of the CD95 and inhibition of EGF-R activation, which results in an inhibition of CD95 tyrosine phosphorylation, CD95 membrane targeting, and DISC formation
Lee et al., Mol Cell Biol 2004 : Remarkably, inhibition of HDAC8 activity by hyperphosphorylation leads to hyperacetylation of histones H3 and H4, suggesting that PKA mediated phosphorylation of HDAC8 plays a central role in the overall acetylation status of histones
Loiselle et al., Am J Physiol Cell Physiol 2004 : Regulation of the human NBC3 Na+/HCO3- cotransporter by carbonic anhydrase II and PKA
Ahi et al., Behav Brain Res 2004 : Inhibition of PKA impaired context- and tone dependent fear conditioning and significantly reduced the phosphorylation of Elk-1, p90Rsk-1 , Erk-1/2, and CREB ... Inhibition of PKA impaired context- and tone dependent fear conditioning and significantly reduced the phosphorylation of Elk-1, p90Rsk-1 , Erk-1/2, and CREB ... Inhibition of PKA impaired context- and tone dependent fear conditioning and significantly reduced the phosphorylation of Elk-1, p90Rsk-1, Erk-1/2, and CREB ... Inhibition of PKA impaired context- and tone dependent fear conditioning and significantly reduced the phosphorylation of Elk-1 , p90Rsk-1, Erk-1/2, and CREB ... Inhibition of PKA impaired context- and tone dependent fear conditioning and significantly reduced the phosphorylation of Elk-1, p90Rsk-1, Erk-1/2 , and CREB ... Inhibition of PKA impaired context- and tone dependent fear conditioning and significantly reduced the phosphorylation of Elk-1, p90Rsk-1, Erk-1/2 , and CREB
Schaar et al., Neuron 2004 : We present evidence that Dcx/microtubule interactions are negatively controlled by Protein Kinase A (PKA) and the MARK/PAR-1 family of protein kinases
Feng et al., FEBS Lett 2004 : PKA phosphorylation and 14-3-3 interaction regulate the function of neurofibromatosis type I tumor suppressor, neurofibromin
Svenningsson et al., Annu Rev Pharmacol Toxicol 2004 (Synaptic Transmission) : Dopamine- and cAMP regulated phosphoprotein , Mr 32 kDa ( DARPP-32 ), was identified initially as a major target for dopamine and protein kinase A (PKA) in striatum
Chio et al., Cell Signal 2004 : PKA dependent activation of PKC, p38 MAPK and IKK in macrophage : implication in the induction of inducible nitric oxide synthase and interleukin-6 by dibutyryl cAMP ... PKA dependent activation of PKC, p38 MAPK and IKK in macrophage : implication in the induction of inducible nitric oxide synthase and interleukin-6 by dibutyryl cAMP
Rius et al., Arterioscler Thromb Vasc Biol 2004 (Calcium Signaling...) : The upregulation of NOR-1 mRNA levels by LDL involves pertusis-sensitive G protein coupled receptors, Ca2+ mobilization, protein kinases A (PKA) and C ( PKC ) activation , and mitogen activated protein kinase pathways ( MAPK ) ( p44/p42 and p38 ) ... The upregulation of NOR-1 mRNA levels by LDL involves pertusis-sensitive G protein coupled receptors, Ca2+ mobilization, protein kinases A (PKA) and C ( PKC ) activation , and mitogen activated protein kinase pathways ( MAPK ) ( p44/p42 and p38 ) ... The upregulation of NOR-1 mRNA levels by LDL involves pertusis-sensitive G protein coupled receptors, Ca2+ mobilization, protein kinases A (PKA) and C ( PKC ) activation , and mitogen activated protein kinase pathways ( MAPK ) ( p44/p42 and p38 )
Layland et al., J Physiol 2004 (Calcium Signaling) : PKA dependent phosphorylation of cardiac troponin I (cTnI) contributes significantly to beta-adrenergic agonist induced acceleration of myocardial relaxation ( lusitropy )
Sirotkin et al., J Reprod Dev 2003 : These observations demonstrate the involvement of IGF-I and OT in the control of ovarian follicular size and follicular cell proliferation, progestagen, estrogen, IGFBP-3, inhibin A and B secretion and in cAMP/PKA- and MAPK/ERK1 dependent intracellular mechanisms
Stempin et al., Eur J Immunol 2004 : Thus, activation of TK, PKA and p38 MAPK by Cz induces an increase of arginase activity in macrophages and the subsequent T. cruzi growth
Cohen et al., Dev Biol 2004 : Protein kinase A (PKA) , known to be active in sperm capacitation, and protein kinase C ( PKC ), involved in the acrosome reaction, can both activate PLD and actin polymerization ... We suggest that PKA- and PKC dependent signal transduction pathways can potentially lead to PLD activation ; however, under physiological conditions, actin polymerization depends primarily on PKA activity
Pedrosa et al., Cell Physiol Biochem 2004 : We have previously shown that the D1-mediated inhibition of Na+-K+-ATPase activity in OK cells involves the sequential activation of the AC-protein kinase A ( AC-PKA ) and the PLC-protein kinase C (PLC-PKC) pathways
Ottaviani et al., Prog Mol Subcell Biol 2004 : PDGF-AB and TGF-beta1 partially inhibit the induced programmed cell death in an insect cell line, and the survival effect is mediated by the activation of phosphatidylinositol 3-kinase, PKA and PKC ... PDGF-AB and TGF-beta1 partially inhibit the induced programmed cell death in an insect cell line, and the survival effect is mediated by the activation of phosphatidylinositol 3-kinase, PKA and PKC ... PDGF-AB and TGF-beta1 partially inhibit the induced programmed cell death in an insect cell line, and the survival effect is mediated by the activation of phosphatidylinositol 3-kinase, PKA and PKC
Rüegg et al., Biochim Biophys Acta 2004 (Neoplasms...) : Endothelial cell COX-2 promotes integrin alphaVbeta3 mediated endothelial cell adhesion, spreading, migration and angiogenesis through the prostaglandin-cAMP-PKA dependent activation of the small GTPase Rac
Boczan et al., J Biol Chem 2004 : Our data predict that syntaphilin could be a highly regulated molecule and that PKA phosphorylation could act as an `` off '' switch for syntaphilin, thus blocking its inhibitory function via the cAMP dependent signal transduction pathway
Dalle et al., J Biol Chem 2004 : Inhibition of either cAMP dependent protein kinase (PKA) or MEK completely blocked ERK1/2 activation by glucagon ... We conclude that glucagon induced ERK1/2 activation is mediated by PKA and that an increase in [ Ca ( 2+ ) ] ( i ) is required for maximal ERK activation
Luconi et al., J Cell Sci 2004 : These results demonstrate that PI 3-kinase negatively regulates sperm motility by interfering with AKAP3-PKA binding, providing the first evidence of a molecular mechanism by which PKA can be targeted to sperm tails by interaction with tyrosine phosphorylated form of AKAP3
Mangiavacchi et al., J Neurochem 2004 : Post-natal rat nucleus accumbens (NAc) cultures were used to study the role of protein kinase A (PKA) in D1 receptor regulation of the surface expression of the AMPA receptor subunit GluR1
Sasaki et al., Inflamm Res 2004 : The inhibitory effect of KF19514 on the GM-CSF production was significantly reduced by a cAMP dependent protein kinase A (PKA) inhibitor, H89
Makarevich et al., Exp Clin Endocrinol Diabetes 2004 : These observations suggest that OT, PKA , and ERKs MAPK can be involved in the control of PGs release and proliferation of ovarian cells ... The influence of OT on both PKA and MAPK, and the ability of PKA and MAPK blockers to prevent completely or partially OT effects suggest, that effects of OT on PGF and PGE can be mediated by both PKA and MAPK
Tsai et al., Endocrinology 2004 : PKA is required for protection and was prevented by H89 ( a PKA inhibitor ), but not PD98059 ( a MAPK kinase inhibitor ) ... These data suggest a mechanism of ERalpha transcriptional activation by PKA that is distinct from E2 activation and that may contribute to the synergistic transcriptional activation of ERalpha by ligand dependent and PKA dependent pathways
Rubic et al., Biochem Pharmacol 2004 : The niacin effect on CD36 but not on ABCA1 was prevented by cyclooxygenase inhibition, whereas the niacin effect on ABCA1 but not on CD36 was prevented by PKA inhibition, suggesting mediation by the 15d-PGJ2/PPARgamma and the cAMP/PKA pathways, respectively ... The niacin effect on CD36 but not on ABCA1 was prevented by cyclooxygenase inhibition, whereas the niacin effect on ABCA1 but not on CD36 was prevented by PKA inhibition, suggesting mediation by the 15d-PGJ2/PPARgamma and the cAMP/PKA pathways, respectively
Uchiya et al., Infect Immun 2004 (Salmonella Infections, Animal) : Taken together, these results indicate that salmonellae cause an SPI-2 dependent increase in PKA activity that leads to CREB phosphorylation, resulting in up-regulation of IL-10 expression in Salmonella infected macrophages
Gomes et al., Eur J Pharmacol 2004 : Dopamine D ( 1 ) -mediated inhibition of Na ( + ), K ( + ) -ATPase activity in opossum kidney ( OK ) cells involves the sequential activation of the adenylyl cyclase-protein kinase A (PKA) and the phospholipase C-protein kinase C ( PKC ) pathways ... It is concluded that dopamine D ( 1 ) -mediated inhibition of NHE3 in renal OK cells involves both adenylyl cyclase-PKA and the phospholipase C-PKC pathways, a mechanism similar to that described for Na ( + ), K ( + ) -ATPase
Cullen et al., Am J Physiol Gastrointest Liver Physiol 2004 : The role of cAMP-GEF and PKA on activation of Akt , a kinase implicated in cAMP survival signaling, was investigated
Nukaga et al., J Periodontol 2004 : The PGE2 stimulated RANKL expression was mediated by EP2/4 and cAMP dependent PKA , while PKC was possibly involved in the PGE2 action
Rao et al., Biol Reprod 2004 : The findings that 1 ) hCG treatment increased cAMP levels and activated protein kinase A (PKA) , 2 ) 8-bromo cAMP mimicked hCG, and 3 ) blocking PKA activation prevented hCG as well as 8-bromo cAMP from increasing both DHEA-ST mRNA and DHEAS levels suggested that cAMP/PKA signaling was involved in the hCG actions
Shimoni et al., Am J Physiol Heart Circ Physiol 2004 (Diabetes Mellitus, Experimental...) : ANG II affects multiple cellular pathways including activation of protein kinase C ( PKC ) and several tyrosine kinases as well as inhibition of protein kinase A (PKA)
Ozkurt et al., Endocrinology 2004 : PTH binding to its receptor activates protein kinase A (PKA) , protein kinase C ( PKC ), and calcium signaling to induce transcription of primary response genes in osteoblasts
Collado et al., Regul Pept 2004 (Neovascularization, Pathologic...) : VIP regulation of VEGF expression was mediated by VPAC(1) receptor and was cAMP/protein kinase A (PKA) dependent
Tokoyoda et al., Int Immunol 2004 : In T cells, the engagement of both the TCR-CD3 complex and the CD28 co-stimulatory molecule also induces cAMP, and subsequently activates PKA
Köhler et al., J Cell Biol 2004 : Rab13 regulates PKA signaling during tight junction assembly
Liu et al., Pancreas 2004 : However, insulin secretion mediated by PKA and PKC activation was desensitized in vanadate treated cells
Kawakami et al., J Pharmacol Sci 2004 (Asthma) : Heterologous H1R down-regulation was significantly reversed in the presence of the cyclic AMP dependent protein kinase (PKA) inhibitor KT5720 ... H1R was phosphorylated by beta2R stimulation ; however, a PKA inhibitor did not inhibit heterologous H1R phosphorylation
Cohen et al., Diabetes 2004 : Mechanistically, although the activity of protein kinase A (PKA) was greatly increased in caveolin-1 null adipocytes, the phosphorylation of perilipin was dramatically reduced, indicating that caveolin-1 may facilitate the PKA mediated phosphorylation of perilipin
Ballabio et al., Cell Mol Life Sci 2004 : Here we report that ( i ) EDF-1 is in vitro and in vivo phosphorylated by protein kinase A (PKA) ; ( ii ) EDF-1/CaM interaction is modulated by the phosphorylation of EDF-1 by PKA ; ( iii ) forskolin stimulates nuclear accumulation of EDF-1 , and ( iv ) PKA phosphorylation enhances EDF-1 interaction with the TATA binding protein
Sakuma et al., Bone 2004 : Treatment with specific inhibitors indicated that PGE ( 2 ), EP2A, and EP4A induced COX-2 expression largely through protein kinase A (PKA)
Endou et al., Am J Physiol Lung Cell Mol Physiol 2004 : We conclude that cAMP/PKA induced Ca2+ desensitization contains at least two mechanisms : 1 ) inhibition of the muscarinic receptor signaling upstream from Rho activation and 2 ) cAMP/PKA 's preferential reversal of PKC mediated Ca2+ sensitization in airway smooth muscle ... To clarify whether cyclic AMP ( cAMP ) /cAMP dependent protein kinase (PKA) activation and Rho-kinase inhibition share a common mechanism to decrease the Ca2+ sensitivity of airway smooth muscle contraction, we examined the effects of 8-bromoadenosine 3',5'-cyclic monophosphate ( 8-BrcAMP ), a stable cAMP analog, and ( + ) - ( R ) -trans-4- ( 1-aminoethyl ) -N- ( 4-pyridyl ) cyclohexane carboxamide dihydrochloride, monohydrate ( Y-27632 ), a Rho-kinase inhibitor, on carbachol ( CCh ) -, guanosine 5'-O- ( 3-thiotriphosphate ) ( GTPgammaS ) -, 4beta-phorbol 12,13-dibutyrate ( PDBu ) -, and leukotriene D4 ( LTD4 ) -induced Ca2+ sensitization in alpha-toxin permeabilized rabbit tracheal and human bronchial smooth muscle
Katoh et al., Mol Cell Endocrinol 2004 : ACTH/PKA dependent nucleocytoplasmic shuttling of SIK1 took place in Y1 cells, implying that the intracellular movement of SIK1 might be a physiologically important determining factor for regulation of steroidogenic gene expression in the early phase of ACTH-stimulation
Park et al., Int Immunopharmacol 2004 : In addition, PGE2 mediated IL-1beta induction was completely inhibited by the cAMP antagonist, Rp-cAMP, and protein kinase A (PKA) inhibitors of KT5720 and H89
Wei et al., Biochemistry 2004 : Site directed mutation of T91 to alanine abolished PKA mediated phosphorylation and inhibition of GAD activity
Jang et al., Exp Mol Med 2004 : These results suggest that both PLD1 and RhoA are phosphorylated by PKA and the interaction between PLD1 and RhoA is inhibited by the phosphorylation of RhoA rather than by the phosphorylation of PLD1 ... These results suggest that both PLD1 and RhoA are phosphorylated by PKA and the interaction between PLD1 and RhoA is inhibited by the phosphorylation of RhoA rather than by the phosphorylation of PLD1
Mundell et al., Mol Pharmacol 2004 : In coimmunoprecipitation experiments, the ability of glutamate to increase association of GRK2 and arrestin-2 with mGluR1a and mGluR1b was inhibited by PKA activation with forskolin ... In coimmunoprecipitation experiments, the ability of glutamate to increase association of GRK2 and arrestin-2 with mGluR1a and mGluR1b was inhibited by PKA activation with forskolin ... Together, these results indicate that PKA activation inhibits the agonist induced internalization and desensitization of mGluR1a and mGluR1b , probably by reducing their interaction with GRK2 and nonvisual arrestins
Tseng et al., J Neurosci 2004 : In contrast, the D2-AMPA interaction involves inhibition of PKA and activation of phospholipase lipase C-IP3 and intracellular Ca2+ at a postsynaptic level
Carlisle Michel et al., Biochem J 2004 : In vitro and cellular experiments demonstrate that PKA-phosphorylation of PDE4D3 on Ser-13 increases the affinity of PDE4D3 for mAKAP
Searle et al., Cell cycle (Georgetown, Tex.) 2004 (Cell Transformation, Neoplastic) : Cdc20 was phosphorylated following DNA damage in a PKA and Mec1 dependent manner, suggesting PKA activation is dependent on Mec1
Hjerrild et al., FEBS Lett 2004 : These results suggest that the transcriptional activity of En-2 is regulated by PKA
Yu et al., J Mol Med (Berl) 2004 : The activity of NF-kappaB can be regulated by protein kinase A (PKA) in a cAMP independent manner ... Our previous results showed that c-MYC induces the activity of PKA by inducing the transcription of the gene encoding the PKA catalytic subunit beta (PKA-Cbeta)
Di Gregorio et al., Am J Physiol Endocrinol Metab 2004 (Insulin Resistance...) : This association between FOXC2 and insulin resistance may be related to the role of FOXC2 in PKA signaling
Sanada et al., Circulation 2004 (Myocardial Infarction) : IP and preischemic PKA activation, but not PKC activation, caused a substantial decrease of Rho-kinase activation during sustained ischemia ... We examined the effect on Rho-kinase activity during sustained ischemia and infarct size of ( 1 ) preischemic transient coronary occlusion ( IP ), ( 2 ) preischemic activation of PKA/PKC , ( 3 ) inhibition of PKA/PKC during IP, and ( 4 ) inhibition of Rho-kinase or actin cytoskeletal deactivation during myocardial ischemia
Liu et al., J Interferon Cytokine Res 2004 : Experimental data indicate that exogenous IFN-gamma stimulated cAMP accumulation and PKA activation in time dependent and dose dependent manners in murine peritoneal macrophages ... Moreover, IFN-gamma stimulated CREB phosphorylation and CREB DNA binding, which could be significantly attenuated by PKA inhibition with H89
Cui et al., J Nutr 2004 : 2HCl , both inhibitors of PKA , did not prevent the fructose induced increases in GLUT5 mRNA abundance and fructose uptake rate
Caesar et al., J Biol Chem 2004 : The involvement of Nat3p and Tfs1p in PKA signaling was supported by caffeine growth inhibition studies
Guerra et al., Pflugers Arch 2004 : Inhibition of either protein kinase A (PKA) or the binding between A-kinase anchoring proteins ( AKAPs ) and the regulatory PKA RII subunit blocked the 2-MeSADP induced activation of CFTR , suggesting that PKA mediates P2Y1 receptor regulation of CFTR through one or more AKAPs ... These data suggest that the X laevis P2Y1 receptor in A6 cells can increase both cAMP/PKA and Ca2+/PKC intracellular levels and that the PKC pathway is involved in CFTR activation via potentiation of the PKA pathway ... These data suggest that the X laevis P2Y1 receptor in A6 cells can increase both cAMP/PKA and Ca2+/PKC intracellular levels and that the PKC pathway is involved in CFTR activation via potentiation of the PKA pathway
Nguyen et al., Cell Signal 2004 (Carcinoma, Hepatocellular...) : Treatment of OVCAR-3 cells with the F-actin stabilizing compound, jasplakinolide, prevented the protein kinase A (PKA) mediated inhibition of SRF ... These results suggest that PKA inhibits LPA stimulation of SRF by promoting the dissolution of F-actin and that this is likely to contribute to the cAMP mediated inhibition of ovarian cancer cell growth ... These results suggest that PKA inhibits LPA stimulation of SRF by promoting the dissolution of F-actin and that this is likely to contribute to the cAMP mediated inhibition of ovarian cancer cell growth
Jaureguiberry et al., Peptides 2004 : The stimulatory effect of ETs on NOS activity was inhibited in the presence of PLC, PKC, PKA and CaMK-II inhibitors ( U-73122, GF-109203X, H-89 and KN-62, respectively ), and the IP3 receptor selective antagonist, 2-APB
Liu et al., Chin Med J (Engl) 2004 : Activation of PKA causes wild type, but not mutant SF-1 to alter its subnuclear distribution pattern to a transactivationally active form ( foci formation )
Li et al., American journal of physiology. Renal physiology 2004 : The stimulatory effect of CHA and CGS-21680 was completely blocked by H89, an inhibitor of protein kinase A (PKA) , or by inhibition of adenylate cyclase with SQ-22536
Jensen et al., Blood 2004 : The protein kinase G ( PKG ) antagonist Rp-8-CPT-cGMPS strongly inhibited PKG mediated 46-kDa VASP Ser239 phosphorylation, but did not inhibit the thrombin induced shape change or the PKA mediated VASP Ser157 phosphorylation
Gray et al., Mol Pharmacol 2004 : Furthermore, specific protein kinase A (PKA) inhibitors blocked IL-1beta and PGE ( 2 ) -induced MUC5AC production ... We conclude that the induction of MUC5AC by IL-1beta, TNF-alpha, PAF, and LPS involves COX-2- generated PGE ( 2 ), activation of EP2 and/or EP4 receptor ( s ), and cAMP-PKA mediated signaling
Thakur et al., J Neurosci 2004 (Synaptic Transmission) : Effects of PKA mediated phosphorylation of Snapin on synaptic transmission in cultured hippocampal neurons ... We investigated the role of PKA dependent phosphorylation of Snapin in hippocampal neurons
Hastings et al., Am J Physiol Cell Physiol 2004 (Lung Neoplasms...) : In summary, PTHrP- ( 1-34 ) and PTHrP- ( 140-173 ) stimulate PKA in lung carcinoma cells and protect cells against UV-induced caspase-3 activation and DNA fragmentation
Montezinho et al., J Neurochem 2004 (Neuroblastoma) : Intracellular Ca2+ chelation, or inhibition of N-type voltage-sensitive Ca2+ channels, or inhibition of cAMP dependent protein kinase (PKA) also abolished the effect of forskolin on Li+ uptake
Hu et al., Neuron 2004 (MAP Kinase Signaling System) : In Aplysia, long-term facilitation (LTF) of sensory neuron synapses requires activation of both protein kinase A (PKA) and mitogen activated protein kinase ( MAPK ) ... Inhibiting PKA during 5-HT applications and inhibiting receptor tyrosine kinase or MAPK during sensorin application blocked both LTF and MAPK activation and translocation
Constantinescu et al., J Biol Chem 2004 (MAP Kinase Signaling System) : This mechanism appears to involve type I PKA dependent phosphorylation of CBP and inhibition of MEK dependent phosphorylation of p90RSK ... This mechanism appears to involve type I PKA dependent phosphorylation of CBP and inhibition of MEK dependent phosphorylation of p90RSK
Lim et al., Am J Physiol Gastrointest Liver Physiol 2005 : The PKA inhibitor KT-5720 ( 0.5 microM ) and milrinone ( 3 microM ), a phosphodiesterase (PDE) III inhibitor , blocked the effect of CO on I ( Ba )
Choudhary et al., Biochem Biophys Res Commun 2004 : In contrast, PKA inhibitors, used at doses that inhibited forskolin stimulated luciferase activity by 90 %, reduced [ Ca(+2) ] ( e ) -stimulated COX-2 mRNA expression and promoter activity by 80-90 %
Snyder et al., J Biol Chem 2004 : Nedd4-2 was a substrate for phosphorylation by PKA in vitro and in cells ; three Nedd4-2 residues were phosphorylated by PKA and were required for cAMP to inhibit Nedd4-2 ( relative functional importance Ser-327 > Ser-221 > Thr-246 )
Lehnart et al., Biochem Biophys Res Commun 2004 (Arrhythmias, Cardiac...) : Calstabin2 binding to RyR2 is regulated by PKA phosphorylation of Ser2809 in RyR2
Hoang et al., J Biol Chem 2004 : Here, we show that GRIP1 is regulated by cAMP dependent protein kinase (PKA) that induces its degradation through the ubiquitin-proteasome pathway ... The proteasome inhibitors MG132 and lactacystin abolished the PKA mediated degradation of GRIP1 ... Using ts20 cells, a temperature-sensitive cell line that contains a thermolabile ubiquitin activating E1 enzyme, it was confirmed that PKA mediated degradation of GRIP1 is dependent upon the ubiquitin-proteasome pathway ... Finally, we show that PKA regulates the intracellular distribution pattern of green fluorescent protein-GRIP1 and stimulates recruitment of GRIP1 to subnuclear foci that are colocalized with the proteasome
Carini et al., Gastroenterology 2004 (Reperfusion Injury) : In the cells treated with CGS21680, PI3K activation was prevented either by inhibiting adenylate cyclase and PKA with, respectively, 2,5-dideoxyadenosine and H89 or by blocking Galphai-protein and Src tyrosine kinase with, respectively, pertussis toxin and PP2 ... In the cells treated with CGS21680, PI3K activation was prevented either by inhibiting adenylate cyclase and PKA with, respectively, 2,5-dideoxyadenosine and H89 or by blocking Galphai-protein and Src tyrosine kinase with, respectively, pertussis toxin and PP2 ... PI3K is activated following hepatocyte hypoxic preconditioning by the combined stimulation of adenosine A 2A receptors, PKA , Galphai protein, and Src
Gentili et al., J Cell Biochem 2004 : Collectively, these data suggest that PTH activates in duodenal cells, a Ca2+ dependent cytosolic PLA2 and attendant AA release and that this activation requires prior stimulation of intracellular ERK1/2, PKA , and PKC
Chen et al., Int J Biochem Cell Biol 2005 (Leydig Cell Tumor...) : Moreover, CS induced the expression of steroidogenic acute regulatory (StAR) protein in dose- and time dependent relationships, and PKA inhibitor, PKC inhibitor, or co-treatment with both inhibitors suppressed it
Li et al., Exp Cell Res 2004 : The protein kinase A (PKA) inhibitor, H-89, completely blocked PGE2 mediated induction of CRE-Luc and c-Fos promoter-Luc promoters, and partially inhibited induction of AP-1-Luc , while the protein kinase C ( PKC ) inhibitor Go-6976 partially inhibited all three promoters, demonstrating substantial cross-talk between these signaling pathways ... The protein kinase A (PKA) inhibitor, H-89, completely blocked PGE2 mediated induction of CRE-Luc and c-Fos promoter-Luc promoters, and partially inhibited induction of AP-1-Luc, while the protein kinase C ( PKC ) inhibitor Go-6976 partially inhibited all three promoters, demonstrating substantial cross-talk between these signaling pathways
Sakurada et al., Adv Perit Dial 2004 : From those findings, we hypothesize that 5 % glucose augments the PKA dependent translocation of AQP1 to the plasma membrane, mediated by PKA translocation to the intracellular AQP1 store
Heijnen et al., J Histochem Cytochem 2004 : The topological proximity of eNOS with the catalytic subunit of PKA in restricted intracellular locations may provide mechanisms for differential PKA mediated eNOS regulation
Kawasaki et al., J Neurosci 2004 (Hyperalgesia) : The ERK activation produced by capsaicin is totally suppressed by inhibition of either protein kinase A (PKA) or PKC
Ciarimboli et al., J Cell Physiol 2004 : hOCT1 expressed in CHO-cells was inhibited by protein kinase A (PKA) activation ( 1 microM forskolin, -58 +/- 6 %, n = 12 ), calmodulin inhibition ( 0.1 microM calmidazolium, -68 +/- 3 %, n = 6 ; 10 microM ophiobolin A, -48 +/- 10 %, n = 7 ), calmodulin dependent kinase II inhibition ( 1 microM KN62, -78 +/- 4 %, n = 12 ), and inhibition of p56 ( lck ) tyrosine kinase ( 10 microM aminogenistein, -35 +/- 7 %, n = 12 ) ... hOCT1 expressed in CHO-cells was inhibited by protein kinase A (PKA) activation ( 1 microM forskolin, -58 +/- 6 %, n = 12 ), calmodulin inhibition ( 0.1 microM calmidazolium, -68 +/- 3 %, n = 6 ; 10 microM ophiobolin A, -48 +/- 10 %, n = 7 ), calmodulin dependent kinase II inhibition ( 1 microM KN62, -78 +/- 4 %, n = 12 ), and inhibition of p56 ( lck ) tyrosine kinase ( 10 microM aminogenistein, -35 +/- 7 %, n = 12 ) ... hOCT1 was inhibited by PKA and endogenously activated by calmodulin, calmodulin dependent kinase II, and p56 ( lck ) tyrosine kinase
Banko et al., J Neurochem 2004 : The NMDA induced activation of Mnk1 and increased phosphorylation of eIF4E were dependent on PKA and ERK, but not PKC, and were present in synaptoneurosome preparations ... The NMDA induced activation of Mnk1 and increased phosphorylation of eIF4E were dependent on PKA and ERK, but not PKC, and were present in synaptoneurosome preparations
Ling et al., Mutat Res 2004 : In contrast, IPP signaling is independent of A2aAR, A2bAR, cAMP or PKA
Francis et al., J Hepatol 2004 : Purified cholangiocytes from normal rats were treated in vitro with forskolin in the absence or presence of Rp-cAMPs ( a PKA inhibitor ), PP2 ( an Src inhibitor ) or PD98059 ( a MEK inhibitor )
Shimomura et al., Arch Biochem Biophys 2004 : PKA activation by 8CPT-2Me-cAMP was 100-fold lower than that by cAMP
Davare et al., J Biol Chem 2004 : Here we report an additional PKA dependent regulation of CaMKK through its interaction with protein 14-3-3
Lee et al., J Neurochem 2004 (Neuroblastoma) : Our results suggest that ERK1/2 and PKA induced regulation of Cdk5 activity possibly through Egr-1, c-fos, and CREB plays a critical role in the RA-induced neuronal differentiation
Sun et al., J Neurophysiol 2004 (Hyperalgesia...) : These results are consistent with the hypothesis that CGRP produces hyperalgesia by a direct action on CGRP1 receptors in the spinal cord dorsal horn and suggest that the effects of CGRP are mediated by both PKA and PKC second-messenger pathways
Rakhilin et al., Science 2004 (Calcium Signaling) : G protein coupled receptor ( GPCR ) -dependent activation of protein kinase A (PKA) led to phosphorylation of RCS at Ser55 and increased its binding to CaM
Luo et al., J Alzheimers Dis 2004 : To further study TRH regulation of tau phosphorylation, immunoblotting was used to explore G-protein coupled TRH receptor activation of the phosphokinase C ( PKC ) and phosphokinase A (PKA) signaling pathways ... Independent of these effects, inhibition of PKA by H89 ( 10 uM ) significantly blocked TRH inhibition of GSK-3 beta ... Independent of these effects, inhibition of PKA by H89 ( 10 uM ) significantly blocked TRH inhibition of GSK-3 beta
Zhang et al., Am J Physiol Gastrointest Liver Physiol 2005 (Hypertension, Portal) : Inhibition of PKA selectively increased myosin phosphorylation to 34.7 +/- 4.18 % ... Acute inhibition of PKA had no effect on RhoA mRNA expression
Harkness et al., Genetics 2004 (Genomic Instability) : While it is known that PKA inhibits APC activity and reduces life span, a link between the Snf1p inhibited Mig1p transcriptional modulator and the APC is novel
Markou et al., J Mol Cell Cardiol 2004 (Cardiomegaly) : Phenylephrine induces activation of CREB in adult rat cardiac myocytes through MSK1 and PKA signaling pathways
Harris et al., Neurosci Lett 2004 : Immunoblot analysis of perirhinal cortical neurons using GluR1 and GluR1-S845 phosphorylation state specific antibodies showed that stimulation of adenylyl cyclase ( AC ) with forskolin ( FSK ) dramatically increased PKA mediated phosphorylation of GluR1-S845
Leblais et al., Circ Res 2004 (Calcium Signaling...) : These results suggest that beta1-AR stimulation activates PI3K via a PKA dependent mechanism, and that G ( betagamma ) and the subsequent activation of betaARK1 are critically involved in the PKA induced PI3K signaling which, in turn, negates cAMP induced positive inotropic effect via inhibiting sarcolemmal Ca2+ influx and the subsequent increase in intracellular Ca2+ transients, without altering the receptor mediated phospholamban phosphorylation, in intact cardiomyocytes ... These results suggest that beta1-AR stimulation activates PI3K via a PKA dependent mechanism, and that G ( betagamma ) and the subsequent activation of betaARK1 are critically involved in the PKA induced PI3K signaling which, in turn, negates cAMP induced positive inotropic effect via inhibiting sarcolemmal Ca2+ influx and the subsequent increase in intracellular Ca2+ transients, without altering the receptor mediated phospholamban phosphorylation, in intact cardiomyocytes
Ferguson et al., Genetics 2005 : Previous studies had proposed that the stress response transcription factors Msn2p/4p, but not Hsf1p , are repressed by PKA
Obara et al., J Cell Sci 2004 : Here, we report that the cAMP dependent protein kinase A (PKA) induced the phosphorylation of Src at residue serine17 ( S17 ) in multiple cell types including PC12, Hek293, AtT-20 and CHO cells ... In these cells, Rap1 is required for cAMP/PKA signaling to ERKs and also for the sustained activation of ERKs by NGF ... In these cells, Rap1 is required for cAMP/PKA signaling to ERKs and also for the sustained activation of ERKs by NGF ... These results strongly indicate that PKA phosphorylation of Src at S17 is essential for cAMP and NGF signaling in PC12 cells and identify PKA as an important downstream target of NGF ... PKA phosphorylation of Src may therefore be required for Rap1 activation in PC12 cells
Min et al., Exp Mol Med 2004 : We investigated the role of protein kinase A (PKA) in microglial activation by both plasminogen and gangliosides in rat primary microglia and in the BV2 immortalized murine microglial cell line ... Both plasminogen and gangliosides activated PKA and increased the DNA binding activity of the cAMP response element- binding protein ( CREB ) ... These results suggest that PKA plays an important role in plasminogen and gangliosides- induced microglial activation
Wan et al., J Gen Physiol 2004 (Calcium Signaling) : We propose that PKA and PKC promote insulin secretion by increasing the number of vesicles that are highly sensitive to Ca ( 2+ )
Hongpaisan et al., J Neurosci 2004 (Calcium Signaling) : Large increases in cytosolic free Ca2+ ( [ Ca2+ ] i ) activate several kinases that are important for neuronal plasticity, including Ca2+/calmodulin dependent kinase II ( CaMKII ), protein kinase A (PKA) , and protein kinase C ( PKC ) ... Large increases in cytosolic free Ca2+ ( [ Ca2+ ] i ) activate several kinases that are important for neuronal plasticity, including Ca2+/calmodulin dependent kinase II ( CaMKII ), protein kinase A (PKA) , and protein kinase C ( PKC )
Rodriguez et al., Anim Reprod Sci 2005 : These results indicate that NO* may be generated by a sperm NOS during heparin induced capacitation and that exogenous NO* acts as a capacitation inducer and involves the participation of PKA , PKC and PTK as part of the intracellular mechanisms that lead to capacitation in cryopreserved bull spermatozoa
Hu et al., J Neurophysiol 2005 (Calcium Signaling) : Our results also suggest that D2R mediated reduction in tonic PKA activity may increase free [ Ca2+ ] in, primarily via disinhibition of IP3Rs
Yamamori et al., J Mol Endocrinol 2004 (Calcium Signaling) : Altogether, we conclude that not only the cAMP/PKA but also the calcium/CaMKIV signaling pathway is involved in the regulation of CRH gene expression ... Furthermore, CREB is thought to be involved in CaMK- as well as cAMP/PKA mediated CRH gene expression
Yeagley et al., Mol Endocrinol 2005 : PKA and Dex also synergistically induced a minimal 3 x glucocorticoid response element promoter, but inhibited Dex induction of the mouse mammary tumor virus and IGF binding protein 1 promoters, even though PKA alone did not regulate these promoters
Lo et al., Cancer Res 2004 (Astrocytoma...) : In MGR3 human glioblastoma cells, PKA and PKC activation resulted in a significant increase in the level of phosphorylation of the GSTP1 protein and was accompanied by a 2.1- and 2.7-fold increase, respectively, in specific GSTP1 activity in the cells ... In normal cells, the functional activation of the GSTP1 protein by PKA- and PKC dependent phosphorylation could represent a potentially important mechanism of cellular protection, whereas in tumors, increased phase II metabolism of anticancer drugs by the more active phosphorylated GSTP1 protein could contribute to the drug resistance and therapeutic failure frequently associated with increased activities of these Ser/Thr kinases
Méndez et al., Mol Cell Endocrinol 2005 : Furthermore, insulin and IGF-I blocked the activation of PKA induced by sLH and these effects were correlated with changes in the total protein content of the catalytic ( C ) and type II regulatory ( RII ) subunits of PKA ... Furthermore, insulin and IGF-I blocked the activation of PKA induced by sLH and these effects were correlated with changes in the total protein content of the catalytic ( C ) and type II regulatory ( RII ) subunits of PKA
Tang et al., Genetics 2004 : Although PDK1 phosphorylates and activates PKC, PKB, and RSK in vivo, PDK1 regulation of PKA remains controversial ... Although PDK1 phosphorylates and activates PKC, PKB, and RSK in vivo, PDK1 regulation of PKA remains controversial
Jia et al., Nature 2004 : Here we show that protein kinase A (PKA) and casein kinase I ( CKI ) regulate Smo cell-surface accumulation and activity in response to Hh. Blocking PKA or CKI activity in the Drosophila wing disc prevents Hh-induced Smo accumulation and attenuates pathway activity, whereas increasing PKA activity promotes Smo accumulation and pathway activation
van der Heyden et al., Cardiovasc Res 2005 (Ion Channel Gating) : Stimulation of beta-adrenergic receptors ( ARs ) results in an increased cyclic adenosine monophosphate ( cAMP ) production by adenylate cyclase ( AC ) and consequently activation of protein kinase (PK) A and phosphorylation of L-type Ca ( 2+ ) channels by this enzyme
Aronoff et al., J Immunol 2005 : By contrast, activation of PKA , but not Epac-1, suppressed AM production of leukotriene B ( 4 ) and TNF-alpha , whereas stimulation of either PKA or Epac-1 inhibited AM bactericidal activity and H ( 2 ) O ( 2 ) production
Ghosh et al., Mol Endocrinol 2005 : cAMP-responsive element binding protein ( CREB ) and Ets-2 form a complex that can be coimmunoprecipitated from choriocarcinoma cells, and association of CREB and Ets-2 is increased by activation of PKA ... cAMP-responsive element binding protein ( CREB ) and Ets-2 form a complex that can be coimmunoprecipitated from choriocarcinoma cells, and association of CREB and Ets-2 is increased by activation of PKA
Sengenes et al., Med Sci (Paris) 2005 : Human fat cell lipolysis was considered until recently to be an exclusive cAMP/protein-kinase A (PKA) regulated metabolic pathway under the control of catecholamines and insulin
Kobayashi et al., J Biol Chem 2005 : RANKL induced degradation of I kappa B alpha and phosphorylation of p38 MAPK and c-Jun N-terminal kinase in RAW264.7 cells were up-regulated by PGE2 in a cAMP dependent protein kinase A (PKA) dependent manner, suggesting that EP2 and EP4 signals cross-talk with RANK signals ... RANKL induced degradation of I kappa B alpha and phosphorylation of p38 MAPK and c-Jun N-terminal kinase in RAW264.7 cells were up-regulated by PGE2 in a cAMP dependent protein kinase A (PKA) dependent manner, suggesting that EP2 and EP4 signals cross-talk with RANK signals
Hildesheim et al., J Biol Chem 2005 (Adenomatous Polyposis Coli...) : Moreover, lack of normal p38 MAPK activity in either Gadd45a-null keratinocytes or in p38 MAPK inhibitor treated keratinocytes leads to decreased CK2 activity and increased PKA activity ... Moreover, lack of normal p38 MAPK activity in either Gadd45a-null keratinocytes or in p38 MAPK inhibitor treated keratinocytes leads to decreased CK2 activity and increased PKA activity
Duca et al., Mol Pharmacol 2005 : The simultaneous inhibition of PKA and PI3K, by N- ( 2- ( p-bromocinnamylamino ) ethyl ) -5-isoquinolinesulfonamide ( H89 ) and 2- ( 4-morpholynil ) -8-phenyl-4H-1-bemzopyran-4-one ( LY294002 ), respectively, blocked MEK1/2 and ERK1/2 phosphorylation, as did lactose, an EBP antagonist ... The simultaneous inhibition of PKA and PI3K, by N- ( 2- ( p-bromocinnamylamino ) ethyl ) -5-isoquinolinesulfonamide ( H89 ) and 2- ( 4-morpholynil ) -8-phenyl-4H-1-bemzopyran-4-one ( LY294002 ), respectively, blocked MEK1/2 and ERK1/2 phosphorylation, as did lactose, an EBP antagonist
Takami et al., FEBS Lett 2005 : Induction of TRANCE expression by IBMX was partially suppressed by the inhibitors of protein kinase A (PKA), ERK, and p38 MAPK, suggesting that activation of ERK and p38 MAPK, as well as PKA , is involved in TRANCE expression by IBMX
Mokashi et al., Arch Biochem Biophys 2005 (Carcinoma, Hepatocellular) : The activation of SPF by protein kinases A (PKA) and Cdelta enhances its ability to stimulate these cholesterolgenic enzymes in microsomal preparations ... To confirm a role for PKA in the regulation of SPF , substitution of alanine for serine-289 ( a putative PKA recognition site ) blocked the stimulation of cholesterol synthesis by SPF ... The activation of SPF by PKA suggests that cholesterol synthesis can be rapidly modulated in response to external stimuli by changes in cAMP levels, and that this regulation is dependent on an as yet undefined interaction with Golgi
Kam et al., Br J Pharmacol 2005 : Interestingly, the PKA inhibitor at the same concentration significantly reduced the increases in PKA activity and Ca2+ channel activity upon beta-AR stimulation in all three groups of rats and the inhibitions were significantly greater in the Ovx rat than in the other two groups of rats
Kim et al., J Mol Endocrinol 2005 : Activation of PKA by forskolin resulted in enhanced androgen induced expression of the PSA gene, an effect that was blocked by the AR antagonist, bicalutamide ... Interestingly, when either p300 or CBP was overexpressed, PKA activation was sufficient to stimulate PSA promoter-driven transcription in the absence of androgen, which was not inhibited by bicalutamide
Bagley et al., Neuron 2005 (Disease Models, Animal...) : We report here that opioid withdrawal in vitro induced an opioid-sensitive cation current that was mediated by the GABA transporter-1 (GAT-1) and required activation of protein kinase A (PKA) for its expression
Szkudelski et al., Physiol Res 2005 : Inhibition of protein kinase A (PKA) by H-89 ( 50 micromol/l ) slightly enhanced leptin release stimulated by glucose and leucine but not by alanine ... Moreover, it has also been shown that inhibition of PKA evokes the opposite effect and enhances leptin release
Ait-Mamar et al., J Biol Chem 2005 : Potentiation of beta2-AR/AC/PKA induced Ca2+ responses by AACOCF3 did not rely on the enhancement of AC activity but was associated with eNOS phosphorylation ( Ser1177 ) and L-NAME-sensitive NO production ... This was correlated with PKA dependent phosphorylation of PLB ( Ser16 ) ... The constraint exerted by the beta2-AR/cPLA2 pathway on the beta2-AR/AC/PKA induced Ca2+ responses required integrity of caveolar structures and was impaired by Filipin III treatment ... Taken together, these data highlight cPLA as a cardiac beta2-AR signaling pathway that limits beta2-AR/AC/PKA induced Ca2+ responses in adult rat cardiomyocytes through the impairment of eNOS activation and PLB phosphorylation
Bai et al., J Biol Chem 2005 (Bone Resorption) : In human MG63 cells, however, we found that ROS promoted heat shock factor 2 (HSF2) binding to heat shock element in human RANKL promoter region and that HSF2, but not PKA , was required for ROS up-regulation of RANKL as revealed by KT5720 and HSF2 RNA interference transfection ... In human MG63 cells, however, we found that ROS promoted heat shock factor 2 (HSF2) binding to heat shock element in human RANKL promoter region and that HSF2, but not PKA , was required for ROS up-regulation of RANKL as revealed by KT5720 and HSF2 RNA interference transfection
Alemán et al., Am J Physiol Endocrinol Metab 2005 (Hepatoblastoma) : However, glucagon can activate the following two possible transduction pathways : protein kinase A (PKA) and protein kinase C ( PKC )
Cho et al., Yonsei Med J 2005 : The induction of ICER mRNA expression by rolipram was suppressed by the inhibitors of protein kinase A (PKA) and p38 MAPK, suggesting the involvement of PKA and p38 MAPK activation in ICER expression by rolipram
Deacon et al., Curr Biol 2005 : Finally, direct inhibition of PKA transiently activates ERK , demonstrating that ERK acts downstream of PKA
Zhang et al., Proc Natl Acad Sci U S A 2005 : Activation of the cAMP pathway, for example, stimulates cellular gene expression by means of the PKA mediated phosphorylation of cAMP-response element binding protein ( CREB ) at Ser-133
Johnson et al., Proc Natl Acad Sci U S A 2005 (Muscular Dystrophy, Duchenne) : These results show that skeletal muscle Ca2+ channel activity and voltage dependent potentiation are controlled by PKA and microfilaments in a convergent manner
Anderson et al., J Cell Physiol 2005 : Immunocytochemistry localises NHERF1 ( NHE3 regulatory factor 1 ) to the apical portion of Caco-2 cells where it will interact with NHE3 and allow PKA mediated phosphorylation of NHE3
Grandjean-Laquerriere et al., Cytokine 2005 : In this study, we use specific inhibitors to investigate the role of both PKA- and PKC mediated pathways in the regulation of UVB induced IL-8 expression in NCTC 2544 cell line ... Finally, we concluded that a cAMP independent PKA activation and a PKC associated pathway are probably involved in the regulation of UVB induced IL-8 synthesis in human keratinocytes
Gerlo et al., Cell Signal 2005 : Furthermore, cAMP induced a PKA dependent phosphorylation of cAMP-responsive element binding protein ( CREB )
Marshall et al., J Exp Biol 2005 : We present a model for rapid control of CFTR and NKCC in chloride cells that includes : ( 1 ) activation of NKCC and CFTR via cAMP/PKA , ( 2 ) activation of NKCC by PKC, myosin light chain kinase (MLCK), p38, OSR1 and SPAK, ( 3 ) deactivation of NKCC by hypotonic cell swelling, Ca ( 2+ ) and an as yet unidentified protein phosphatase and ( 4 ) involvement of protein tyrosine kinase ( PTK ) acting on FAK to set levels of NKCC activity
Ha et al., J Leukoc Biol 2005 : Further characterization of the response to PSG17 indicated that cyclic adenosine monophosphate dependent protein kinase A (PKA) is involved in the up-regulation of IL-10 and IL-6, and it is not required for the induction of TGF-beta(1) ... Further characterization of the response to PSG17 indicated that cyclic adenosine monophosphate dependent protein kinase A (PKA) is involved in the up-regulation of IL-10 and IL-6 , and it is not required for the induction of TGF-beta(1)
Subramaniam et al., J Neurosci 2005 : IGF-1 activated the protein kinase A (PKA) to mediate ERK inhibition via c-Raf phosphorylation at an inhibitory site ( Ser259 ) ... This indicates a selective role for PKA in ERK inhibition through c-Raf-Ser259 phosphorylation
Lécureuil et al., Mol Endocrinol 2005 : Here, we report that, in primary cultures of Sertoli cells isolated from prepubertal rats, FSH enhanced p70S6K enzymatic activity, in a PKA dependent manner
Nishimura et al., Clinical calcium 2001 : The decrease in the Ca ( 2+ ) sensitivity involves the PKA mediated inhibition of myosin light chain kinase , the PKG mediated activation of MLCP, and PKA- or PKG mediated inactivation of rhoA
Déliot et al., Am J Physiol Cell Physiol 2005 : Moreover, in mouse kidney slices, PTH induced an increase in NHERF1 phosphorylation ; independent activation of PKA or PKC also resulted in increased phosphorylation of NHERF1 in kidney slices
Yoshida et al., J Neurosci 2005 : Consistently, constitutively active PKA or CREB stimulated VAMP2-EGFP puncta formation
Fukushima et al., J Dent Res 2005 : The cAMP/PKA inhibitors Rp-cAMP, H89, and PKI did not affect PTHrP induced TRAP+ cell formation
Thams et al., Eur J Endocrinol 2005 : In islets cultured at 5.5 mmol/l glucose, and then perifused in physiological Krebs-Ringer medium, the PKA inhibitors, H89 ( 10 micromol/l ) and PKI 6-22 amide ( 30 micromol/l ) did not inhibit glucose ( 16.7 mmol/l ) -induced insulin secretion, but inhibited stimulation by the adenylyl cyclase activator, forskolin ( 10 micromol/l )
Hasler et al., J Am Soc Nephrol 2005 : These results indicate that in collecting duct principal cells : ( 1 ) a short-term increase of extracellular osmolarity decreases AQP2 expression through inhibition of AQP2 gene transcription ; ( 2 ) a long-term increase of extracellular tonicity, but not osmolarity, enhances AQP2 expression via stimulation of AQP2 gene transcription ; and ( 3 ) long-term hypertonicity and PKA increases AQP2 expression through synergistic but independent mechanisms
Maas et al., J Neurosci 2005 (Ataxia) : These results indicate that the unique activation of PKA signaling mediated by the calcium stimulated ACs is an important component of the neuronal response to ethanol
Pasapera et al., J Steroid Biochem Mol Biol 2005 : Transactivation of estrogen-sensitive genes by FSH or PKA activators were blocked ( approximately 90 % ) by H89 ( PKA inhibitor ) and LY294002 but not by Wortmannin ( PI3-K inhibitors ), 4-OH-tamoxifen, ICI182,780 or SB203580 ( p38 MAPK inhibitor ) ; PD98059 ( ERK1/2 inhibitor ) partially ( approximately 30 % ) blocked the FSH mediated effect ... Transactivation of estrogen-sensitive genes by FSH or PKA activators were blocked ( approximately 90 % ) by H89 ( PKA inhibitor ) and LY294002 but not by Wortmannin ( PI3-K inhibitors ), 4-OH-tamoxifen, ICI182,780 or SB203580 ( p38 MAPK inhibitor ) ; PD98059 ( ERK1/2 inhibitor ) partially ( approximately 30 % ) blocked the FSH mediated effect
Golin-Bisello et al., Am J Physiol Cell Physiol 2005 : We used STa, cell-permeant cGMP, and cAMP agonists in conjunction with PKG and PKA inhibitors, respectively, in rat jejunum to examine whether 1 ) cGMP and cGK II regulate the translocation of CFTR to the apical membrane and its relevance to fluid secretion, and 2 ) PKA regulates cAMP dependent translocation of CFTR because this intestinal segment is a primary target for toxigenic diarrhea ... We used STa, cell-permeant cGMP, and cAMP agonists in conjunction with PKG and PKA inhibitors, respectively, in rat jejunum to examine whether 1 ) cGMP and cGK II regulate the translocation of CFTR to the apical membrane and its relevance to fluid secretion, and 2 ) PKA regulates cAMP dependent translocation of CFTR because this intestinal segment is a primary target for toxigenic diarrhea
Jungmann et al., J Biol Chem 2005 (Glioma) : In rat C6 glioma cells, post-transcriptional gene regulation occurs through PKA mediated stabilization of LDH-A mRNA and subsequent increase of intracellular LDH-A mRNA levels
Miyabe et al., Neurosci Lett 2005 (Sciatic Neuropathy) : These data suggest that early, sciatic ligation elicited phosphorylation of CREB in the spinal dorsal horn is mediated by multiple kinase pathways, and that PKA , PKC and CaMKII activate CREB at least in part by way of the ERK pathway
Agarwal et al., Brain Res Mol Brain Res 2005 : Pituitary adenylate cyclase activating polypeptide ( PACAP ) mimics neuroendocrine and behavioral manifestations of stress : Evidence for PKA mediated expression of the corticotropin releasing hormone (CRH) gene
Malki et al., EMBO J 2005 : We find that activation of cAMP dependent protein kinase A (PKA) induces phosphorylation of SOX9 on its two S64 and S181 PKA sites, and its nuclear localization by enhancing SOX9 binding to the nucleocytoplasmic transport protein importin beta
Brennesvik et al., Cell Signal 2005 : Inhibition of PKA by H89 decreased adrenaline stimulated glycogen phosphorylase activation but increased PKB activation, which further supports that adrenaline increases insulin stimulated PKB phosphorylation via Epac
Nanmoku et al., Neurosci Lett 2005 (Second Messenger Systems) : Furthermore, urocortin induced changes in TH-mRNA and activity were inhibited by H89 ( a PKA inhibitor ) and SQ22536 as well as alpha-helical CRF- ( 9-41 )
Hur et al., J Cell Biol 2005 (Calcium Signaling) : The IP ( 3 ) R complex functions as a focal point to promote Ca ( 2+ ) release in two ways : ( 1 ) it facilitates PKA dependent phosphorylation of IP(3)R1 in response to BK-induced elevation of cAMP, and ( 2 ) it couples the plasmalemmal EGFR with IP(3)R1 at the Ca ( 2+ ) store located juxtaposed to the plasma membrane
Ji et al., Mol Cell Biol 2005 (Second Messenger Systems) : In NC cultures, high-level activation of cAMP signaling mediates protein kinase A (PKA) dependent Rap1-B-Raf-ERK1/2 activation, leading to cytoplasmic accumulation of phospho-Smad1, thus terminating bone morphogenetic protein 2 (BMP2) induced sympathoadrenal cell development ... In NC cultures, high-level activation of cAMP signaling mediates protein kinase A (PKA) dependent Rap1-B-Raf-ERK1/2 activation, leading to cytoplasmic accumulation of phospho-Smad1, thus terminating bone morphogenetic protein 2 (BMP2) induced sympathoadrenal cell development ... In NC cultures, high-level activation of cAMP signaling mediates protein kinase A (PKA) dependent Rap1-B-Raf-ERK1/2 activation, leading to cytoplasmic accumulation of phospho-Smad1, thus terminating bone morphogenetic protein 2 (BMP2) induced sympathoadrenal cell development
Moon et al., J Neurosci Res 2005 : In contrast, the Epac-specific cAMP analog 8- ( 4-chloro-phenylthio ) -2'-O-methyladenosine-3',5'-cyclic monophosphate ( 8CPT-2Me-cAMP ) activates Rap1 in the BV2 cells, but does not induce PKA activation, as judged by CREB phosphorylation
Yao et al., Proc Natl Acad Sci U S A 2005 (Heroin Dependence) : AGS3 and Galpha ( i3 ) knockdown prevents opiate activation of PKA signaling ... Thus, Galpha ( i3 ) /betagamma/AGS3 appears to mediate mu opiate receptor activation of PKA signaling as well as heroin seeking behavior
Wang et al., Biochem Biophys Res Commun 2005 : Our recent studies demonstrate the functional relevance of PKA mediated phosphorylation of ezrin in parietal cell secretion [ R. Zhou, X. Cao, C. Watson, Y. Miao, Z. Guo, J.G. Forte, X. Yao, Characterization of protein kinase A-mediated phosphorylation of ezrin in gastric parietal cell activation, J. Biol. Chem. 278 ( 2003 ) 35651 ]
Fernández et al., J Mol Endocrinol 2005 : Studies in GH4C1 cells, with an expression vector for Rap1 GTPase activating protein ( Rap1 GAP1 ), demonstrated reduced VIP induced MAPK activation, indicating that VIP dependent activation of the extracellular signal regulated kinase ( ERK ) pathway requires PKA-Rap1 signaling ... IGF-I and VIP stimulation of CRE mediated transcription required activation of both Ras-MAPK and cAMP/PKA signaling ... IGF-I and VIP stimulation of CRE mediated transcription required activation of both Ras-MAPK and cAMP/PKA signaling
Halls et al., Ann N Y Acad Sci 2005 (MAP Kinase Signaling System) : The reporter gene studies suggest that ligand stimulation of LGR7 and LGR8 involves cAMP-PKA and p38/JNK signaling ... The reporter gene studies suggest that ligand stimulation of LGR7 and LGR8 involves cAMP-PKA and p38/JNK signaling
Yang et al., Life Sci 2005 : We found : ( a ) in the absence of Ca ( 2+ ) /CaM, PKA slightly phosphorylated MLC(20) and stimulated the Mg ( 2+ ) -ATPase activity of myosin , which was strengthened significantly by arachidonic acid ( ACAD ) ; ( b ) Ca ( 2+ ) -independent phosphorylation of myosin by PKA was obviously less efficient than both Ca ( 2+ ) -dependent and independent phosphorylation of myosin by MLCK ; ( c ) micro-amount of calponin could not increase the precipitation of myosin phosphorylated by PKA, but it increased the precipitation of myosin phosphorylated by MLCK, suggesting the presence of conformational differences between the myosins phosphorylated by PKA and by MLCK
Obara et al., Mol Pharmacol 2005 (Astrocytoma) : Furthermore, both CREB and IL-6 promoter activities were suppressed by SB203580 [ 4- ( 4-fluorophenyl ) -2- ( 4-methylsulfinylphenyl ) -5- ( 4-pyridyl ) -1H-imidazole ], a p38 mitogen activated protein kinase ( MAPK ) inhibitor, and H89 [ N- [ 2- ( 4-bromocinnamylamino ) -ethyl ] -5-isoquinoline ], a protein kinase A (PKA) inhibitor, indicating involvements of p38 MAPK and PKA in CREB activation and IL-6 expression ... Furthermore, both CREB and IL-6 promoter activities were suppressed by SB203580 [ 4- ( 4-fluorophenyl ) -2- ( 4-methylsulfinylphenyl ) -5- ( 4-pyridyl ) -1H-imidazole ], a p38 mitogen activated protein kinase ( MAPK ) inhibitor, and H89 [ N- [ 2- ( 4-bromocinnamylamino ) -ethyl ] -5-isoquinoline ], a protein kinase A (PKA) inhibitor, indicating involvements of p38 MAPK and PKA in CREB activation and IL-6 expression
Mordasini et al., American journal of physiology. Renal physiology 2005 : The effects of AVP on Na pump current and Na-K-ATPase cell surface expression were dependent on PKA activity but independent of increased apical Na ( + ) entry ... Therefore, stimulation of Na-K-ATPase cell surface expression by AVP is dependent on PKA but does not rely on alpha ( 1 ) -subunit phosphorylation on serine 943 in the collecting duct principal cells
Zheng et al., Pharmacol Ther 2005 (Heart Failure) : More importantly, persistent beta1AR stimulation evokes a multitude of cardiac toxic effects, including myocyte apoptosis and hypertrophy, via a calmodulin dependent protein kinase II ( CaMKII ) -, rather than cAMP-PKA- , dependent mechanism in rodent heart in vivo and cultured cardiomyocytes
Dejda et al., Pharmacol Rep 2005 (Neurodegenerative Diseases) : The mechanism underlying neuroprotective properties of PACAP seems to involve activation of adenylyl cyclase ( AC ) -- > cyclic adenosine 3',5'-mono-phosphate ( cAMP ) -- > protein kinase A (PKA) and mitogen activated protein ( MAP ) kinase pathways, and inhibition of caspase-3
Valverde et al., J Biol Chem 2005 : When CaM kinase II was blocked by its specific inhibitor KN-93, the Ca2+-ATPase activity decreased to the levels measured in the absence of calmodulin ; the unusually high Ca2+ affinity dropped 2-fold ; and the PKA mediated stimulation of Ca2+-ATPase was no longer seen ... When CaM kinase II was blocked by its specific inhibitor KN-93, the Ca2+-ATPase activity decreased to the levels measured in the absence of calmodulin ; the unusually high Ca2+ affinity dropped 2-fold ; and the PKA mediated stimulation of Ca2+-ATPase was no longer seen
Hughes-Fulford et al., Cell Signal 2005 : Gene expression of IL-2R ( alpha ) and IFN(gamma) was dependent on PKA in S49 wt cells but not in kin- mutants ... Gene expression of IL-2R ( alpha ) and IFN(gamma) was dependent on PKA in S49 wt cells but not in kin- mutants ... Taken together, these results suggest that the PKA in addition to PKC and MAPK pathways plays a role in early T-cell activation and induction of IL-2, IL-2R ( alpha ) and IFN(gamma) gene expression ... Taken together, these results suggest that the PKA in addition to PKC and MAPK pathways plays a role in early T-cell activation and induction of IL-2, IL-2R ( alpha ) and IFN(gamma) gene expression ... Taken together, these results suggest that the PKA in addition to PKC and MAPK pathways plays a role in early T-cell activation and induction of IL-2, IL-2R ( alpha ) and IFN(gamma) gene expression
Giordano et al., J Neurochem 2005 : PKA mediated phosphorylation of matrin 3 is followed by its degradation ... NMDA receptor activation in rat brain in vivo by ammonia injection also induced PKA mediated matrin 3 phosphorylation and degradation in brain cell nuclei ... These results suggest that PKA mediated phosphorylation of matrin 3 may serve as a rapid way of transferring information from synapses containing NMDA receptors to neuronal nuclei under physiological conditions, and may contribute to neuronal death under pathological conditions
Knerr et al., FEBS Lett 2005 (Anoxia) : Stimulation of GCMa and syncytin via cAMP mediated PKA signaling in human trophoblastic cells under normoxic and hypoxic conditions ... Stimulation of GCMa and syncytin via cAMP mediated PKA signaling in human trophoblastic cells under normoxic and hypoxic conditions
Lynch et al., J Biol Chem 2005 : Knockdown of PDE4D, but not PDE4B, amplifies isoprenaline stimulated phosphorylation of the beta2-adrenergic receptor ( beta2-AR ) by PKA and activation of extracellular signal regulated kinase ( ERK ) through G ( i )
Hubbard et al., Cell Immunol 2005 : Cyclic AMP dependent protein kinase (PKA) played a major role in this enhancement since the PKA inhibitor, H89, blocked secretion of Ang2
Kagan et al., Novartis Found Symp 2005 (Long QT Syndrome) : Elevations of cAMP as seen in beta-adrenergic stimulation can result in PKA dependent phosphorylation of hERG and direct binding of cAMP to the channel protein
Phelps et al., Calcif Tissue Int 2005 : Selective activation of cAMP-PKA signaling with, 10 microM forskolin ( FSK ) and PKC signaling with 1 microM phorbol 12-myristate 13-acetate ( PMA ) significantly increased RAMP3 mRNA levels, whereas 1 microM ionomycin ( a calcium ionophore ) had no effect
Qin et al., J Cell Biochem 2005 : In the present report, by using different PTH peptide fragments, protein kinase activators, and inhibitors, we have demonstrated that PTH regulates amphiregulin in a cAMP-protein kinase A (PKA) dependent manner both in vitro and in vivo ... In the present report, by using different PTH peptide fragments, protein kinase activators, and inhibitors, we have demonstrated that PTH regulates amphiregulin in a cAMP-protein kinase A (PKA) dependent manner both in vitro and in vivo
Shimono et al., Diabetes Res Clin Pract 2005 : Neither GTP nor activated PKA in the absence of PKC activation increased insulin release under Ca2+ depleted condition in the presence of ATP, but both enhanced insulin secretion in the presence of ATP when PKC was activated
Andoh et al., Mol Pharmacol 2005 : The induction of Trx was blocked by protein kinase A (PKA) inhibitor and mediated by a PKA-sensitive phospho-activation of mitogen activated protein ( MAP ) kinase Erk1/2 and the transcription factor c-Myc
Gerdjikov et al., Eur J Neurosci 2005 : In previous studies inhibition or activation of cyclic adenosine monophosphate dependent protein kinase (PKA) blocked NAc amphetamine produced CPP
Sindić et al., Kidney Int 2005 : Depolarizations were inhibited by arachidonic acid or inhibition of phospholipase A2 (PLA2) , but not by protein kinase A (PKA) inhibition
Nashiki et al., Kidney Int 2005 : Furthermore, PTH activated both protein kinase A (PKA) and protein kinase Calpha (PKCa) and increased the phosphorylation of 250 kD and 80 kD substrates ; this latter substrate was identified as ezrin, which a member of the ezrin-radixin-moesin (ERM) protein family
Akimova et al., J Physiol 2005 : The rank order of potency ( 2MeSATP > ATP > ADP > > UTP ) indicates that P 2Y1 rather than P 2Y2 receptors contribute to PKA and I ( SC ) activation ... The rank order of potency ( 2MeSATP > ATP > ADP > > UTP ) indicates that P 2Y1 rather than P 2Y2 receptors contribute to PKA and I ( SC ) activation ... Viewed collectively, these data show that Cl- secretion in C7-MDCK monolayers treated with basolateral ATP is triggered by P 2Y1 receptors and is mediated by subsequent [ Ca2+ ] i-independent activation of PLA2 and PKA
Bouchard et al., Endocrinology 2005 (Breast Neoplasms) : PKA mediated phosphorylation increases the interaction between GATA3 and LRH-1 and the requirement for PKA in aromatase PII promoter stimulation involves at least three specific amino acid residues : GATA3 Ser308, GATA4 Ser261, and LRH-1 Ser469 ... PKA mediated phosphorylation increases the interaction between GATA3 and LRH-1 and the requirement for PKA in aromatase PII promoter stimulation involves at least three specific amino acid residues : GATA3 Ser308, GATA4 Ser261, and LRH-1 Ser469 ... PKA mediated phosphorylation increases the interaction between GATA3 and LRH-1 and the requirement for PKA in aromatase PII promoter stimulation involves at least three specific amino acid residues : GATA3 Ser308, GATA4 Ser261, and LRH-1 Ser469
Wang et al., J Clin Invest 2005 : Treatments that prevented release of Ca2+ from intracellular stores or activation of PKA blocked ATPgammaS stimulated changes in capacitance
Belcher et al., Endocrinology 2005 : Inhibition of Galphai signaling or protein kinase A (PKA) activity blocked the ability of ICI182,780 to rapidly stimulate ERK signaling
Somekawa et al., Circ Res 2005 : Here we demonstrated that the gating function of GJ is enhanced by the protein kinase A (PKA) dependent signal, and that the accumulation of connexin43 (Cx43) , the most abundant Cx in myocytes, is enhanced by an exchange protein directly activated by cAMP ( Epac ) ( Rap1 activator ) -dependent signal
Delghandi et al., Cell Signal 2005 : Our results suggest that forskolin induced CREB phosphorylation and activation in NIH 3T3 cells is mediated directly by PKA and by a time delayed PKA dependent p38/MSK-1 pathway
Dhakras et al., American journal of physiology. Renal physiology 2006 : The latter process may result from a PKA dependent phosphorylation of AUF1
Wu et al., Molecular pain 2005 (Pain) : This study was designed to use Western blot analysis to investigate the role of mitogen activated protein ( MAP ) /extracellular signal regulated kinase ( ERK ) kinase ( MEK 1/2 ), PKA and PKC in regulating the phosphorylation of CREB in the spinal cord of rats following intraplantar capsaicin injection
Andersson et al., J Neurosci 2005 (Catalepsy) : Our data indicate that a considerable proportion of the psychomotor effect of cannabinoids can be accounted for by a signaling cascade in striatal projection neurons involving PKA dependent phosphorylation of DARPP-32 , achieved via modulation of dopamine D2 and adenosine A2A transmission
Chang et al., Mol Cell Biol 2005 : We found that acetylation of these residues is required to protect GCMa from ubiquitination and increases the TAD stability with a concomitant increase in transcriptional activity, supporting the importance of acetylation in PKA dependent GCMa activation
Howe et al., Proc Natl Acad Sci U S A 2005 : Importantly, inhibition of PKA activity or its ability to interact with A kinase anchoring proteins inhibited the activity of the Rac GTPase within pseudopodia
Dodge-Kafka et al., Nature 2005 (Hypertrophy) : Anchored PKA stimulates PDE4D3 to reduce local cAMP concentrations, whereas an mAKAP associated ERK5 kinase module suppresses PDE4D3
Oh et al., J Ethnopharmacol 2006 : The expression of HO-1 protein by ALE was reduced by pretreatment with LY83583 and ODQ, specific inhibitors of guanylate cyclase, but not by PKA inhibitors, H89 and KT5720, indicating that PKG signaling pathway regulates HO-1 induction by ALE
Ding et al., Proc Natl Acad Sci U S A 2005 : PDE3A down-regulation activated cAMP/PKA signaling, leading to ICER up-regulation via PKA dependent stabilization of ICER ... PDE3A down-regulation activated cAMP/PKA signaling, leading to ICER up-regulation via PKA dependent stabilization of ICER
Misra et al., Biol Psychiatry 2006 : Interestingly, PKA activator coinfusion with PKA inhibitor into the NAc shell significantly normalized the PKA inhibitor induced decreases in the protein levels of PKA-Calpha and p-CREB as well as of NPY in the NAc shell of rats ... Interestingly, PKA activator coinfusion with PKA inhibitor into the NAc shell significantly normalized the PKA inhibitor induced decreases in the protein levels of PKA-Calpha and p-CREB as well as of NPY in the NAc shell of rats ... Interestingly, PKA activator coinfusion with PKA inhibitor into the NAc shell significantly normalized the PKA inhibitor induced decreases in the protein levels of PKA-Calpha and p-CREB as well as of NPY in the NAc shell of rats
Sanghi et al., Regul Pept 2005 : Inhibition of protein kinase G ( PKG ) with KT5823 blunted ANP ( 1-28 ) -induced upregulation of renin, but not Ao mRNA, while inhibition of protein kinase A (PKA) with KT5720 attenuated the upregulation of both renin and Ao mRNA
Shiga et al., Neurosci Res 2005 : Our results showed that CREB phosphorylation is principally regulated by PKA activity in progenitor cells but not by PKC activity, and that this phosphorylation advances the differentiation of progenitor cells to immature cells in oligodendrocytes
Hino et al., Mol Cell Biol 2005 : Prostaglandin E ( 1 ) ( PGE ( 1 ) ), isoproterenol, and dibutyryl cAMP ( Bt ( 2 ) cAMP ), all of which activate PKA, increased the cytoplasmic and nuclear beta-catenin protein level, and these actions were suppressed by a PKA inhibitor and RNA interference for PKA ... Although PKA did not affect the formation of a complex between glycogen synthase kinase 3beta ( GSK-3beta ), beta-catenin, and Axin, phosphorylation of beta-catenin by PKA inhibited ubiquitination of beta-catenin in intact cells and in vitro ... Ser675 was found to be a site for phosphorylation by PKA, and substitution of this serine residue with alanine in beta-catenin attenuated inhibition of the ubiquitination of beta-catenin by PKA, PKA induced stabilization of beta-catenin, and PKA dependent activation of Tcf ... Ser675 was found to be a site for phosphorylation by PKA, and substitution of this serine residue with alanine in beta-catenin attenuated inhibition of the ubiquitination of beta-catenin by PKA, PKA induced stabilization of beta-catenin , and PKA dependent activation of Tcf
Wand et al., J Clin Invest 2005 (Alcohol-Induced Disorders, Nervous System...) : Alcohol intake by these animals was shown to increase PKA dependent CREB phosphorylation and thereby NPY expression, subsequently ameliorating anxiety-like behavior ... Alcohol intake by these animals was shown to increase PKA dependent CREB phosphorylation and thereby NPY expression, subsequently ameliorating anxiety-like behavior
Guerra et al., J Biol Chem 2005 (Cystic Fibrosis) : Importantly, wt NHERF1 overexpression in confluent DeltaF508 CFBE41o- and DeltaF508 CFT1-C2 cell monolayers induced both a significant redistribution of CFTR from the cytoplasm to the apical membrane and a PKA dependent activation of CFTR dependent chloride secretion ... Importantly, wt NHERF1 overexpression in confluent DeltaF508 CFBE41o- and DeltaF508 CFT1-C2 cell monolayers induced both a significant redistribution of CFTR from the cytoplasm to the apical membrane and a PKA dependent activation of CFTR dependent chloride secretion
Mantovani et al., J Clin Endocrinol Metab 2005 (Adenoma...) : Moreover, in group 2, PKA blockade by the specific inhibitor PKI increased cyclin D1 expression ( 96 +/- 25 % over basal ) and ERK1/2 activity ( 116 +/- 28 % over basal ) ... Moreover, in group 2, PKA blockade by the specific inhibitor PKI increased cyclin D1 expression ( 96 +/- 25 % over basal ) and ERK1/2 activity ( 116 +/- 28 % over basal )
Kiermayer et al., Mol Biol Cell 2005 (Pheochromocytoma) : The present study showed that specific stimulation of PKA triggered ERK1/2 activation that was considerably more transient than that observed upon simultaneous activation of both PKA and Epac ... Activation of Epac appeared to extend the duration of PKA dependent ERK1/2 activation and converted cAMP from a proliferative into an anti-proliferative, neurite outgrowth promoting signal
Boonyaratanakornkit et al., FASEB J 2005 : Since NF-kappaB, AP-1, and CREB are all regulated by PKA and are transcription factors predicted by microarray analysis to be involved in the altered gene expression in vectorless gravity, the data suggest that PKA is a key player in the loss of T-cell activation in altered gravity ... Since NF-kappaB, AP-1 , and CREB are all regulated by PKA and are transcription factors predicted by microarray analysis to be involved in the altered gene expression in vectorless gravity, the data suggest that PKA is a key player in the loss of T-cell activation in altered gravity ... Since NF-kappaB , AP-1, and CREB are all regulated by PKA and are transcription factors predicted by microarray analysis to be involved in the altered gene expression in vectorless gravity, the data suggest that PKA is a key player in the loss of T-cell activation in altered gravity
Yoon et al., FEBS Lett 2005 : To investigate the involvement of protein kinase A (PKA) , Src, and Ras in 8-Br-cAMP induced PLD activation, we used PKA inhibitor, H89 and Rp-cAMPs, and transfections of DN-Src and DN-Ras ... H-89 and Rp-cAMPs completely blocked 8-Br-cAMP mediated PLD and ERK activation, implying the involvement of PKA in this PLD activation
Mattson et al., J Neurochem 2005 (Cocaine-Related Disorders) : Therefore, enhanced activation of ERK, but not PKA , enables and mediates cocaine induced CREB phosphorylation in nucleus accumbens of rats that are sensitized by repeated cocaine administration outside their home cages
Gambaryan et al., Am J Physiol Endocrinol Metab 2006 : In in vitro kinase assays and intact cells, we could show that cAMP induced activation of CaMK , using the adenylate cyclase activator forskolin or the cAMP-analog Sp-5,6-DCI-cBIMPS ( cBIMPS ), was not mediated by PKA
Hokari et al., Am J Physiol Gastrointest Liver Physiol 2005 : VIP induced cAMP response element binding protein ( CREB ) /ATF1 phosphorylation, and this was prevented by treatment with inhibitors of either MEK or p38 and by PKA and MSK1 inhibitor H89 ... VIP induced cAMP response element binding protein ( CREB ) /ATF1 phosphorylation, and this was prevented by treatment with inhibitors of either MEK or p38 and by PKA and MSK1 inhibitor H89 ... VIP induced cAMP response element binding protein ( CREB ) /ATF1 phosphorylation, and this was prevented by treatment with inhibitors of either MEK or p38 and by PKA and MSK1 inhibitor H89
Keeley et al., Biotechniques 2005 (MAP Kinase Signaling System) : The novel hybrid transcription factor TetR-Elk-1 was regulated by MAPK ERK kinase 1 (MEK-1) overexpression, and TetR-CREB was regulated by protein kinase A (PKA) overexpression or elevation of cyclic AMP levels ... The novel hybrid transcription factor TetR-Elk-1 was regulated by MAPK ERK kinase 1 (MEK-1) overexpression, and TetR-CREB was regulated by protein kinase A (PKA) overexpression or elevation of cyclic AMP levels ... The novel hybrid transcription factor TetR-Elk-1 was regulated by MAPK ERK kinase 1 (MEK-1) overexpression, and TetR-CREB was regulated by protein kinase A (PKA) overexpression or elevation of cyclic AMP levels
Rondaij et al., Arterioscler Thromb Vasc Biol 2006 : These results suggest that perinuclear clustering of WPBs involves PKA dependent regulation of the dynein-dynactin complex ... Together, our results suggest that epinephrine induced clustering of WPBs involves PKA dependent regulation of the dynein-dynactin complex
Houslay et al., Biochem Soc Trans 2005 : Beta-arrestin recruited phosphodiesterase-4 desensitizes the AKAP79/PKA mediated switching of beta2-adrenoceptor signalling to activation of ERK
Chen et al., Experimental biology and medicine (Maywood, N.J.) 2005 (Prostatic Neoplasms) : Results of this experiment indicated that cAMP/PKA inhibited RhoA activation, and serine188 phosphorylation on RhoA was necessary for PKA to exert its inhibitory effect on RhoA activation
Fassett et al., Mol Biol Cell 2006 : Inhibition of PKA using H89 increased cell spreading on collagen gel in an EGF dependent manner, whereas activation of PKA using 8-Br-cAMP decreased cell spreading on collagen film ... PKA inhibition also restored ERK activation, cyclin D1 expression and G1-S progression on collagen gel, but had no effect on cells adherent to collagen film ... PKA inhibition also restored ERK activation, cyclin D1 expression and G1-S progression on collagen gel, but had no effect on cells adherent to collagen film ... PKA inhibition also restored ERK activation, cyclin D1 expression and G1-S progression on collagen gel, but had no effect on cells adherent to collagen film
Soto et al., J Neurochem 2006 : Our results suggest that FGF-2 acts on RGCs via FGFR1, activating the ERK pathway and CREB to increase BDNF synthesis, and PKA and CREB to increase TrkB synthesis
Dixit et al., Circ Res 2005 : A dominant negative SHP2 mutant prevented the activation of PKA and phosphorylation of eNOS without affecting that of Akt
Chang et al., Cell Signal 2006 (MAP Kinase Signaling System) : LTA induced iNOS expression and NO release were inhibited by a non-selective COX inhibitor ( indomethacin ), a selective COX-2 inhibitor ( NS-398 ), an adenylyl cyclase ( AC ) inhibitor ( dideoxyadenosine, DDA ), and a protein kinase A (PKA) inhibitor ( KT-5720 ) ... Furthermore, both PGE2 and the direct PKA activator, dibutyryl-cAMP, also induced iNOS expression in a concentration dependent manner
Song et al., Hepatology 2006 : Reporter assays confirmed that cAMP and protein kinase A (PKA) inhibited human CYP7A1 gene transcription, in contrast to their stimulation of the PEPCK gene
Zhang et al., Endocrinology 2006 : ERK MAPK was also induced by the heat treatment in a time- and protein kinase A (PKA) dependent manner
Gu et al., FEBS Lett 2006 : Moreover, activation of PKA decreases and inhibition of PKA increases the dimerization of 14-3-3zeta and the functional interaction of 14-3-3zeta with p53
Namkoong et al., Exp Mol Med 2005 : These results clearly show that PGE2 increased angiogenesis by activating the NO/cGMP signaling pathway through PKA/PI3K/Akt dependent increase in eNOS activity
Chin et al., Exp Mol Med 2005 (Neoplasms) : We also revealed that the combined treatment of radiation and the inhibitor of protein kinase A (PKA) to these radioresistant cells resulted in synergistic inhibition of DNA-PK , Rad51 and Bcl-2 expressions of the cells, and consequently restored radiosensitivity of the cells ... We also revealed that the combined treatment of radiation and the inhibitor of protein kinase A (PKA) to these radioresistant cells resulted in synergistic inhibition of DNA-PK, Rad51 and Bcl-2 expressions of the cells, and consequently restored radiosensitivity of the cells ... We also revealed that the combined treatment of radiation and the inhibitor of protein kinase A (PKA) to these radioresistant cells resulted in synergistic inhibition of DNA-PK, Rad51 and Bcl-2 expressions of the cells, and consequently restored radiosensitivity of the cells
No et al., Biol Pharm Bull 2006 (Melanoma, Experimental) : Results showed that 44'-BP effectively suppressed the amounts of tyrosinase and MITF proteins, cAMP levels, and PKA activation
Endoh et al., Br J Pharmacol 2006 : 7. These results suggest that mu- and kappa-opioid receptors and ORL-1 receptor inhibit N- and P/Q-type VDCCs via G alpha ( i ) -protein betagamma subunits, whereas CB1 receptors inhibit L-type VDCCs via G alpha ( i ) -proteins involving PKA in NTS
Honegger et al., Proc Natl Acad Sci U S A 2006 : Both PKA and EPAC inhibited NHE3 activity without inducing changes in the expression of the transporter in BBM ... Activation of PKA , but not of EPAC, led to an increase of NHE3 phosphorylation
Fujita et al., Blood 2006 (Endotoxemia...) : Unlike normal DCs, which produced proinflammatory cytokines in response to bacterial lipopolysaccharide (LPS), DC ( regs ) produced fewer proinflammatory cytokines and instead preferentially produced interleukin-10 (IL-10), and these events involved the expression of IkappaBNS and Bcl-3 as well as cyclic AMP ( cAMP ) -mediated activation of protein kinase A (PKA) ... Unlike normal DCs, which produced proinflammatory cytokines in response to bacterial lipopolysaccharide (LPS), DC ( regs ) produced fewer proinflammatory cytokines and instead preferentially produced interleukin-10 (IL-10), and these events involved the expression of IkappaBNS and Bcl-3 as well as cyclic AMP ( cAMP ) -mediated activation of protein kinase A (PKA)
Kimata-Hayashi et al., Endocr J 2005 (Pituitary Neoplasms...) : PKA inhibitor attenuated adrenomedullin induced prolactin release
Kawakami et al., Biochim Biophys Acta 2006 : From these results presented here, it is concluded that ( i ) p37 ( FGF-BP ) may be tightly associated with bLF in bovine milk ; and ( ii ) the physiological correlation between p37 and bLF may be regulated by the PKA mediated full phosphorylation of p37 or by the direct binding of sulfatide to p37 in vivo ... From these results presented here, it is concluded that ( i ) p37 ( FGF-BP ) may be tightly associated with bLF in bovine milk ; and ( ii ) the physiological correlation between p37 and bLF may be regulated by the PKA mediated full phosphorylation of p37 or by the direct binding of sulfatide to p37 in vivo ... It was found, in vitro, that ( i ) p37 had a higher binding affinity with bFGF than bLF ; ( ii ) p37 functioned as a phosphate acceptor for at least three protein kinases ( PKA, CK1 and CK2); (iii ) bLF stimulated about 3-fold the PKA mediated phosphorylation of p37 , but suppressed its phosphorylation by CK1 ; and ( iv ) galloyl pedunculagin was an effective inhibitor for the phosphorylation of p37 by PKA and CK1
Han et al., Cell cycle (Georgetown, Tex.) 2006 : As as result of these regulatory loops, the Cdc2/cyclin B complex is maintained in an inactive state by the two-way PKA dependent activation of Wee1 and inactivation of Cdc25
Vanhoose et al., J Neurosci 2006 : After investigating the effect of coactivation of the NMDAR and the Gs-coupled beta-adrenergic receptor on GluR1 phosphorylation state, we have observed a novel signal that prevents PKA mediated phosphorylation of GluR1 at serine site 845
Jin et al., Biochem Biophys Res Commun 2006 : In addition, our study shows that PKA mediated phosphorylation of ezrin is essential and sufficient for the apical localization of WWOX protein as disruption of ezrin-WWOX interaction eliminated the apical localization of WWOX
Zhao et al., Cell Immunol 2005 (MAP Kinase Signaling System) : Although gAd partially increased cAMP concentration and PKA activity, it directly reduced leptin induced ERK1/2 and p38 MAPK phosphorylation thus inhibiting TNF-alpha production
Calipel et al., J Biol Chem 2006 (Cell Transformation, Neoplastic...) : This ruled out a role for Rap1 in the PKA mediated B-Raf/ERK activation in ( WT ) B-Raf cells ... Pharmacological inhibition of cAMP dependent protein kinase (PKA) and siRNA mediated depletion of PKA greatly reduced B-Raf activity, ERK1/2 activation, and cell proliferation in ( WT ) B-Raf cells, whereas it did not affect ( V600E ) B-Raf cells, demonstrating a key role of PKA in mediating ( WT ) B-Raf/ERK signaling for uveal melanoma cell growth ... Pharmacological inhibition of cAMP dependent protein kinase (PKA) and siRNA mediated depletion of PKA greatly reduced B-Raf activity, ERK1/2 activation, and cell proliferation in ( WT ) B-Raf cells, whereas it did not affect ( V600E ) B-Raf cells, demonstrating a key role of PKA in mediating ( WT ) B-Raf/ERK signaling for uveal melanoma cell growth ... Pharmacological inhibition of cAMP dependent protein kinase (PKA) and siRNA mediated depletion of PKA greatly reduced B-Raf activity, ERK1/2 activation, and cell proliferation in ( WT ) B-Raf cells, whereas it did not affect ( V600E ) B-Raf cells, demonstrating a key role of PKA in mediating ( WT ) B-Raf/ERK signaling for uveal melanoma cell growth ... Pharmacological inhibition of cAMP dependent protein kinase (PKA) and siRNA mediated depletion of PKA greatly reduced B-Raf activity, ERK1/2 activation, and cell proliferation in ( WT ) B-Raf cells, whereas it did not affect ( V600E ) B-Raf cells, demonstrating a key role of PKA in mediating ( WT ) B-Raf/ERK signaling for uveal melanoma cell growth ... This ruled out a role for Rap1 in the PKA mediated B-Raf/ERK activation in ( WT ) B-Raf cells
Millen et al., Eur J Cell Biol 2006 (Anoxia...) : Despite the hypoxia induced increases in the expression of PDE4A10/11 , PDE4B2 and PDE4D5 and activation of certain of these long PDE4 isoforms through PKA phosphorylation, we suggest that the failure to see any overall increase in PDE4 activity is due to ERK mediated phosphorylation and inhibition of particular PDE4 long isoforms ... Despite the hypoxia induced increases in the expression of PDE4A10/11, PDE4B2 and PDE4D5 and activation of certain of these long PDE4 isoforms through PKA phosphorylation, we suggest that the failure to see any overall increase in PDE4 activity is due to ERK mediated phosphorylation and inhibition of particular PDE4 long isoforms ... Despite the hypoxia induced increases in the expression of PDE4A10/11, PDE4B2 and PDE4D5 and activation of certain of these long PDE4 isoforms through PKA phosphorylation, we suggest that the failure to see any overall increase in PDE4 activity is due to ERK mediated phosphorylation and inhibition of particular PDE4 long isoforms
Reddy et al., J Membr Biol 2005 : CFTR is classically activated by PKA plus millimolar ATP, but cytosolic glutamate activation of gCFTR is independent of ATP and phosphorylation
Citterio et al., Proc Natl Acad Sci U S A 2006 : Thus, two more critical elements of BIG1 molecular structure were identified, as well as the potential function of microtubules in a novel PKA effect on BIG1 translocation
Van Nuland et al., Mol Microbiol 2006 : Trehalase activation upon ammonium re-supplementation is dependent on PKA activity, but not on its regulatory subunit nor is it associated with an increase in cAMP
Taurin et al., J Biol Chem 2006 : In the present study, we have shown that ( i ) beta-catenin can be phosphorylated by protein kinase A (PKA) in vitro and in intact cells at two novel sites, Ser-552 and Ser-675 ; ( ii ) phosphorylation by PKA promotes the transcriptional activity ( TCF/LEF transactivation ) of beta-catenin ; ( iii ) mutation of Ser-675 attenuates the promoting effect of PKA ; ( iv ) phosphorylation by PKA does not affect the GSK3 dependent phosphorylation of beta-catenin , its stability, or intracellular localization ; and ( v ) phosphorylation at the Ser-675 site promotes the binding of beta-catenin to its transcriptional coactivator, CREB binding protein
Mitsushima et al., Genes Cells 2006 : Inhibition of protein kinase A (PKA) activity suppressed the vinexin induced increase in WAVE2 protein, while activation of PKA increased WAVE2 expression without vinexin beta ... These results suggest that vinexin beta regulates the proteasome dependent degradation of WAVE2 in a PKA dependent manner
Tobimatsu et al., Endocrinology 2006 : The PKA activator, forskolin, and the PKC activator, phorbol 12-myristate-13-acetate, as well as the PTH analog, [ Nle ( 8,18 ), Tyr ( 34 ) ] human PTH- ( 3-34 ) amide, all increased beta-catenin levels ... In conclusion, the data demonstrate that PTH stimulates osteoblast beta-catenin levels via Smad3, and that both PKA and PKC pathways are involved ... In conclusion, the data demonstrate that PTH stimulates osteoblast beta-catenin levels via Smad3, and that both PKA and PKC pathways are involved
Bodnar et al., Circ Res 2006 : Previous work showed that CXCR3 ligandation initiates protein kinase A (PKA) phosphorylation dependent inhibition of m-calpain , required for induced cell motility, in fibroblasts but not epithelial cells ... Previous work showed that CXCR3 ligandation initiates protein kinase A (PKA) phosphorylation dependent inhibition of m-calpain , required for induced cell motility, in fibroblasts but not epithelial cells ... Here we show that CXCR3 activation in endothelial cells induces an increase in cAMP and PKA activation ... These data indicate that the activation of CXCR3 inhibits endothelial tube formation through a PKA mediated inhibition of m-calpain
Park et al., Dev Biol 2006 : As a negative component of PCP signaling, PKA inhibits not only the activation of RhoA and JNK but also the Dsh-Daam1-RhoA complex formation which is essential for the regulation of RhoA activity ... As a negative component of PCP signaling, PKA inhibits not only the activation of RhoA and JNK but also the Dsh-Daam1-RhoA complex formation which is essential for the regulation of RhoA activity
Gesty-Palmer et al., J Biol Chem 2006 (MAP Kinase Signaling System) : Furthermore, stimulation of hPTH1R with PTH analogues, [ Trp1 ] PTHrp- ( 1-36 ) and [ d-Trp12, Tyr34 ] PTH- ( 7-34 ), selectively activated G ( s ) /PKA mediated ERK1/2 activation or G protein-independent/beta-arrestin dependent ERK1/2 activation, respectively
Li et al., J Am Soc Nephrol 2006 (Diabetes Insipidus, Nephrogenic...) : Surprising, the effect of lithium was cAMP independent ; it did not alter AVP stimulated cAMP production or PKA dependent phosphorylation of AQP2 or cAMP responsive element binding protein ... Surprising, the effect of lithium was cAMP independent ; it did not alter AVP stimulated cAMP production or PKA dependent phosphorylation of AQP2 or cAMP responsive element binding protein
Abderrahmani et al., EMBO J 2006 (Hyperglycemia) : We demonstrate that the transcriptional repressor ICER , which is induced in a PKA dependent manner by chronic hyperglycemia and cAMP raising agents, is responsible for the decline of the four genes
Xu et al., Br J Pharmacol 2006 : 6 Our results show that ADM in the RVLM exerts an inhibitory effect on baroreflex activity via an ADM receptor mediated mechanism, and that activation of PKA is involved in this event
Collado-Hidalgo et al., Brain Behav Immun 2006 (HIV Infections) : This effect was specific to Type I interferons and driven by PKA mediated repression of IFNA and IFNB gene transcription ... This effect was specific to Type I interferons and driven by PKA mediated repression of IFNA and IFNB gene transcription
Wang et al., Mol Cell Biol 2006 : Using a small interfering RNA approach, we demonstrate that C3G is required for the activation of ERKs and Rap1 by cAMP/PKA
Paul et al., Am J Physiol 1991 : Our results indicate that although the signaling pathways triggered by alpha- and beta-adrenergic agonists converge at the level of adenylate cyclase in tracheal serous cells, PKA and PKC independently regulate CSPG secretion
O'Flaherty et al., Free radical research 2006 : Taken together these results suggest that LDH-C4 and NADP-ICDH contribute with the redox status to support bovine sperm capacitation and that PKA , PKC and PTK are involved in different mechanisms induced by different inducers that lead bovine spermatozoa to be capacitated
Boo et al., Exp Mol Med 2006 : Shear stress stimulated the phosphorylation of cAMP responsive element binding protein ( CREB ) in a PKA dependent manner
Friedrichsen et al., J Endocrinol 2006 : We conclude that incretin induced beta-cell replication is dependent on cAMP/PKA , p42 MAPK and PI3K activities, which may involve transcriptional induction of cyclin D1
Orihuela et al., J Endocrinol 2006 (MAP Kinase Signaling System) : Inhibition of adenylyl cyclase by i.b. injection of SQ 22536 blocked the increase of IP3 levels induced by E ( 2 ), while inhibition of PLC by ET-18-OCH ( 3 ) had no effect on E ( 2 ) -induced PKA activity ... Thus, activation of PLC-IP3 by E ( 2 ) requires previous stimulation of cAMP-PKA
Cokic et al., Blood 2006 : We found that hydroxyurea dose- and time-dependently induced rapid and transient phosphorylation of eNOS at Ser1177 in a PKA dependent manner ; inhibitors of PKB/Akt could partially abrogate this effect
He et al., Biochim Biophys Acta 2006 : Lipolysis is primarily regulated by protein kinase A (PKA) , which phosphorylates perilipin and hormone-sensitive lipase (HSL), and causes translocation of HSL from cytosol to lipid droplets in adipocytes
Rehfuss et al., J Biol Chem 1991 : Of these CRE binding proteins, only CREB has been shown to be activated by cAMP dependent protein kinase A (PKA) , and as such, CREB represents the only basis for our understanding of cAMP regulated transcriptional activity
Hinken et al., J Appl Physiol 2006 : Myocytes ( n = 9 ) from nine Ex pigs had comparable force production but a approximately 30 % increase in peak power output compared with myocytes ( n = 8 ) from eight Sed. Interestingly, Ex myofibrillar samples also had higher baseline PKA induced phosphorylation levels of cardiac troponin I , which may contribute to the increase in power
Hasegawa et al., Arterioscler Thromb Vasc Biol 2006 : DDAH2 transfection increased nuclear and threonine-phosphorylation levels of Sp1 in a protein kinase A (PKA) dependent manner ... DDAH2 increased Sp1 DNA binding activity was PKA dependent
Miyoshi et al., J Biol Chem 2006 : HSL dependent lipolysis in response to catecholamines is mediated by protein kinase A (PKA) dependent phosphorylation of perilipin A (Peri A) , an essential lipid droplet (LD) associated protein ... PKA stimulated HSL translocation was confirmed in differentiated brown adipocytes from perilipin null mice expressing an adipose-specific Peri Adelta1-6 transgene ... Thus, PKA induced HSL translocation was independent of perilipin phosphorylation ... Using adipocytes retrovirally engineered from murine embryonic fibroblasts of perilipin null mice ( Peri-/- MEF ), we demonstrate by cell fractionation and confocal microscopy that up to 50 % of cellular HSL is LD-associated in the basal state and that PKA stimulated HSL translocation is fully supported by adenoviral expression of a mutant perilipin lacking all six PKA sites ( Peri Adelta1-6 ) ... Thus, the lipolytic action ( s ) of HSL at the LD surface requires PKA dependent perilipin phosphorylation ... This suggests that PKA dependent perilipin phosphorylation facilitates ( either direct or indirect ) perilipin interaction with LD-associated HSL
Sengenes et al., Med Sci (Paris) 2005 : Human fat cell lipolysis was considered until recently to be an exclusive cAMP/protein-kinase A (PKA) regulated metabolic pathway under the control of catecholamines and insulin
Ikari et al., J Cell Sci 2006 : PKA inhibitors, N- [ 2- ( p-bromocinnamylamino ) ethyl ] -5-isoquinolinesulfonamide dihydrochloride ( H-89 ) and myristoylated protein kinase A inhibitor 14-22 amide PKI, and an adenylate cyclase inhibitor, 2',5'-dideoxy adenosine ( DDA ), reduced the phosphoserine level of PCLN-1 ... Thus, the PKA dependent phosphorylation of Ser217 in PCLN-1 is essential for its localization in the TJ and transepithelial Mg2+ transport
Villa et al., Am J Physiol Cell Physiol 2006 (Femoral Fractures) : CGRP ( 10 ( -8 ) M ) enhanced protein kinase A (PKA) activity in hOB, and hOB exposure to the PKA inhibitor, H89 ( 2 x 10 ( -6 ) M ), abolished the inhibitory effect of CGRP on OPG secretion
Muñiz et al., Biol Reprod 2006 : Using primary cultures of rat granulosa cells and immortalized mouse granulosa cells, we demonstrate a mechanism for the regulation of cyclin D2 at the level of transcription via a PKA dependent signaling mechanism
Jones et al., Biophys J 2006 (Calcium Signaling) : We show in vitro that cAMP dependent protein kinase (PKA) is inhibited by high [Ca2+ ] through a shift in the ratio of CaATP/MgATP toward CaATP
Li et al., Biochem Pharmacol 2006 : Inhibition of cAMP dependent PKA with H89 ( 1 microM ) or PLC with U73122 ( 1 microM ) also markedly attenuated the phosphorylation of ERK 1/2 induced by glucagon ( glucagon + U73122 : 109+/-15 % ; glucagon + H89 : 113+/-16 % ; p < 0.01 versus glucagon ) or Ang II ( Ang II + U73122 : 111+/-13 % ; Ang II + H89 : 86+/-10 % ; p < 0.01 versus Ang II ) ... Inhibition of cAMP dependent PKA with H89 ( 1 microM ) or PLC with U73122 ( 1 microM ) also markedly attenuated the phosphorylation of ERK 1/2 induced by glucagon ( glucagon + U73122 : 109+/-15 % ; glucagon + H89 : 113+/-16 % ; p < 0.01 versus glucagon ) or Ang II ( Ang II + U73122 : 111+/-13 % ; Ang II + H89 : 86+/-10 % ; p < 0.01 versus Ang II )
Breitbart et al., Mol Cell Endocrinol 2006 : Protein kinase A (PKA) and C ( PKC ) can both activate sperm PLD and actin polymerization under in vitro incubation, however under physiological conditions, actin polymerization depends primarily on PKA activity
Chen et al., Eur J Cell Biol 2006 : We have found that the functional regulation of the I ( Ks ) channel by PKA requires the A kinase anchoring protein ( AKAP ) Yotiao
Weber et al., Bone 2006 (MAP Kinase Signaling System) : Since PTH ( 1-34 ) activates both Adenylate Cyclase/Protein Kinase A ( AC/PKA ) and Protein Kinase C ( PKC ) downstream of the PTH1R in osteoblastic cells, we independently determined the effect of either pathway on Jagged1
Mattiazzi et al., Braz J Med Biol Res 2006 (Acidosis...) : These two prerequisites appear to be fulfilled by beta-adrenoceptor stimulation, which as a result of PKA activation, triggers the activation of CaMKII by increasing intracellular Ca2+ , and inhibits PP1
Küntziger et al., Biochemistry 2006 : PKA binding to AKAP149 in vitro is unaffected by the presence of PKC or PP1 , and similarly, PKC binding is independent of PKA or PP1
Jafri et al., J Biol Chem 2006 : While agonist dependent ERK1/2 activation by the NK1 receptor was independent of protein kinase A (PKA) or PKC activity, NK1-betaArr1 mediated ERK1/2 activation was completely inhibited when basal PKA and PKC activity were blocked ... While agonist dependent ERK1/2 activation by the NK1 receptor was independent of protein kinase A (PKA) or PKC activity, NK1-betaArr1 mediated ERK1/2 activation was completely inhibited when basal PKA and PKC activity were blocked
Laag et al., Int J Cancer 2006 (Adenocarcinoma...) : Using a cell line derived from a human pulmonary adenocarcinoma with Clara cell phenotype ( PACC ) and immortalized human small airway epithelial cells ( HPLD1 ), the putative cells of origin of this cancer type, our current studies have analyzed signaling initiated by binding of NNK to the beta 1-AR. NNK upregulated ERK1/2 and CREB/ATF-1 phosphorylation in a PKA dependent manner in both cell lines ... Using a cell line derived from a human pulmonary adenocarcinoma with Clara cell phenotype ( PACC ) and immortalized human small airway epithelial cells ( HPLD1 ), the putative cells of origin of this cancer type, our current studies have analyzed signaling initiated by binding of NNK to the beta 1-AR. NNK upregulated ERK1/2 and CREB/ATF-1 phosphorylation in a PKA dependent manner in both cell lines ... Using a cell line derived from a human pulmonary adenocarcinoma with Clara cell phenotype ( PACC ) and immortalized human small airway epithelial cells ( HPLD1 ), the putative cells of origin of this cancer type, our current studies have analyzed signaling initiated by binding of NNK to the beta 1-AR. NNK upregulated ERK1/2 and CREB/ATF-1 phosphorylation in a PKA dependent manner in both cell lines
Leake et al., J Struct Biol 2006 : The softening effect of PKA on N2B-titin may require specific conditions/factors present inside the cardiomyocytes
Park et al., Mol Cells 2006 : The p38 mitogen activated protein kinase ( MAPK ) inhibitor, SB203580, phosphatidylinositol 3-kinase (PI-3K) inhibitor, LY294003, protein kinase A (PKA) inhibitor, H89, and protein kinase C ( PKC ) inhibitor, chelerythrine, had no effect on activation of promoter IV by NGF
Yao et al., Proc Natl Acad Sci U S A 2006 (Heroin Dependence) : In this article, we present evidence that MOR and CB1 act synergistically on cAMP/PKA signaling in NAc/striatal neurons
Bolger et al., Biochem J 2006 : siRNA ( small interfering RNA ) -mediated knockdown of RACK1 in HEK-293 ( human embryonic kidney ) B2 cells increased beta-arrestin scaffolded PDE4D5 approx. 5-fold, increased PDE4D5 recruited to the beta2AR ( beta2-adrenergic receptor ) upon isoproterenol challenge approx. 4-fold and severely attenuated ( approx. 4-5 fold ) both isoproterenol stimulated PKA ( protein kinase A ) phosphorylation of the beta2AR and activation of ERK ( extracellular-signal regulated kinase ) ... siRNA ( small interfering RNA ) -mediated knockdown of RACK1 in HEK-293 ( human embryonic kidney ) B2 cells increased beta-arrestin scaffolded PDE4D5 approx. 5-fold, increased PDE4D5 recruited to the beta2AR ( beta2-adrenergic receptor ) upon isoproterenol challenge approx. 4-fold and severely attenuated ( approx. 4-5 fold ) both isoproterenol stimulated PKA ( protein kinase A ) phosphorylation of the beta2AR and activation of ERK ( extracellular-signal regulated kinase )
Hazan-Eitan et al., Blood 2006 : Inhibition of PKA during PLB differentiation prevented Fc gammaRIIA expression, whereas dibutyryl cAMP ( dbcAMP ) induced its expression in both PLB and PLB-D cells
Gonzalez et al., Mol Cell Biol 1991 : Cyclic AMP mediates the hormonal stimulation of a number of eukaryotic genes by directing the protein kinase A (PK-A) dependent phosphorylation of transcription factor CREB ... Remarkably, a glutamine-rich region near the N terminus appeared to be important for PK-A mediated induction of CREB since removal of this domain caused a marked reduction in CREB activity
Lee et al., Glia 2006 : The H2S induced calcium elevation is partly attenuated by H-89, a selective cAMP dependent protein kinase (PKA) inhibitor , but not by U73122, a phospholipase C (PLC) inhibitor, and chelerythrine, a selective protein kinase C ( PKC ) inhibitor, suggesting the involvement of cAMP/PKA, but not PLC/PKC/phosphoinositol-3,4,5-inositol ( IP3 ) pathway
Guergnon et al., Apoptosis 2006 (Theileriasis) : A PKA survival pathway inhibited by DPT-PKI , a new specific cell permeable PKA inhibitor , is induced by T. annulata in parasitized B-lymphocytes ... DPT-PKI specifically inhibited PKA activity in bovine cell extracts and, as expected, also inhibited the PKA dependent survival of T. annulata parasitized TBL3 cells
Huang et al., J Neurosci 2006 : These Ng-/- mice, as compared with Ng+/+, respond poorly after treatment of their hippocampal slices with agents that activate signaling molecules important for learning and memory, including Ca2+/calmodulin dependent protein kinase II ( alphaCaMKII ), PKC, protein kinase A (PKA) , extracellular signal regulated kinase ( ERK ), and cAMP response element binding protein ( CREB ) ... These Ng-/- mice, as compared with Ng+/+, respond poorly after treatment of their hippocampal slices with agents that activate signaling molecules important for learning and memory, including Ca2+/calmodulin dependent protein kinase II ( alphaCaMKII ), PKC, protein kinase A (PKA) , extracellular signal regulated kinase ( ERK ), and cAMP response element binding protein ( CREB )
Chen et al., J Immunol 2006 : These results suggest that PGN induced IL-6 production involves COX-2 generated PGE ( 2 ), activation of the EP2 and EP4 receptors, cAMP formation, and the activation of PKA , protein kinase C, p38 MAPK, IKKdbeta, kinase alphabeta, p65 phosphorylation, and NF-kappaB ... PGN mediated IL-6 production was inhibited by a nonselective cyclooxygenase (COX) inhibitor ( indomethacin ), a selective COX-2 inhibitor ( NS398 ), a PGE ( 2 ) ( EP2 ) antagonist ( AH6809 ), a PGE ( 4 ) ( EP4 ) antagonist ( AH23848 ), and a protein kinase A (PKA) inhibitor ( KT5720 ), but not by a nonselective NO synthase inhibitor ( N ( G ) -nitro-l-arginine methyl ester )
Amadesi et al., J Physiol 2006 (Calcium Signaling...) : Thus, PAR2 activates PKCepsilon and PKA in sensory neurons, and thereby sensitizes TRPV1 to cause thermal hyperalgesia
Weber et al., J Neurochem 2006 (Calcium Signaling) : These studies and analysis of constitutively active forms of kinases revealed that cyclic nucleotide/protein kinase A (PKA) , calcium/calmodulin dependent kinase ( CaMK ) II and Ras/mitogen activated protein kinase ( MAPK ) regulate CLK/CYC activity
Melling et al., J Mol Cell Cardiol 2006 (MAP Kinase Signaling System) : Recently, we reported that administration of a protein kinase A (PKA) inhibitor suppressed exercise induced hsp70 gene expression, suggesting a role for PKA in the regulation of HSF1 activation in vivo ... While the mechanism by which PKA regulates HSF1 is unclear, studies in vitro have reported that HSF1 is phosphorylated on two serine residues by mitogen activated protein kinases ( MAPKs ) ; ERK1/2 ( ser307 ) and JNK/SAPK ( ser363 ) ... Inhibition of this PKA mediated increase in ERK1/2 phosphorylation through the simultaneous administration of an ERK1/2 inhibitor ( UB-1026 ; 0.25 mg/kg ) restored exercise induced hsp70 mRNA levels in PKA inhibited rats that previously demonstrated a suppressed response ( P < 0.05 ) ... Given that ERK1/2 has been shown to be a negative regulator of HSF1 in vitro, these results suggest a role for ERK1/2 in the PKA mediated regulation of HSF1 activation following exercise
Hayashida et al., J Biol Chem 2006 : However, TGFbeta induction of syndecan-1 was inhibited by transient expression of a dominant negative construct of protein kinase A (PKA) and by specific inhibitors of PKA
Shimo et al., J Bone Miner Res 2006 (Bone Neoplasms...) : Furthermore, we found that CCN2 was induced by PTHrP through PKA- , PKC-, and ERK mediated pathways therein ... CCN2 was critically involved in osteolytic metastasis and was induced by PKA- and PKC dependent activation of ERK1/2 signaling by PTHrP ... CCN2 was critically involved in osteolytic metastasis and was induced by PKA- and PKC dependent activation of ERK1/2 signaling by PTHrP
Li et al., Clinical calcium 2006 (Osteochondrodysplasias) : Signaling transduction through the PTH/PTHrP receptor has two possible pathways : the activation of adenylate cyclase and subsequent protein kinase A (PKA) , and the activation of phospholipase C (PLC)
Favia et al., Biochem Biophys Res Commun 2006 : NHE3 inhibits PKA dependent functional expression of CFTR by NHERF2 PDZ interactions ... We observe here that co-expression of NHE3 reduced both PKA dependent apical CFTR expression and its activation once in place by approximately 50 % ... When only CFTR is expressed on the apical membrane, deletion of any of the NHERF2 binding domains inhibited both PKA dependent apical CFTR expression and its activation, while when NHE3 was co-expressed with CFTR PDZ2 deletion was without effect on CFTR sorting and activity
Leung et al., Int J Cardiol 2007 : Pharmacological studies revealed that ENT-1 activity was stimulated by PKA and PKC-delta/epsilon, however, ENT-2 activity was unaffected
Miura et al., Toxicol Appl Pharmacol 2006 : Triphenyltin impairs a protein kinase A (PKA) dependent increase of cytosolic Na+ and Ca2+ and PKA independent increase of cytosolic Ca2+ associated with insulin secretion in hamster pancreatic beta-cells
Chen et al., Kidney Int 2006 (Uremia) : The results demonstrate that the cyclic AMP ( cAMP ) /protein kinase A (PKA) , but not protein kinase C, signaling pathway is involved in uremic serum induced RUNX2 expression and ALP activity in BVSMCs ... The results demonstrate that the cyclic AMP ( cAMP ) /protein kinase A (PKA) , but not protein kinase C, signaling pathway is involved in uremic serum induced RUNX2 expression and ALP activity in BVSMCs
Umenishi et al., Biochim Biophys Acta 2006 : PKA activity was stimulated by AVP but PKA inhibitors did not block the upregulation of AQP2 expression ... PKA activity was stimulated by AVP but PKA inhibitors did not block the upregulation of AQP2 expression
Bolton et al., Glia 2006 : This study provides evidence that the heterogeneous RMP of mature astrocytes is regulated by cAMP and PKA signaling
Taskén et al., Biochem Soc Trans 2006 : cAMP inhibits Src-family kinase signalling by PKA ( protein kinase A ) -mediated phosphorylation and activation of Csk ( C-terminal Src kinase )
Charest et al., Cell Signal 2007 : Here we report that this activating pathway is independent of G alpha s, G alpha i, G alpha q or G betagamma and that the V2R mediated activation of G alpha s inhibits ERK1/2 activity in a cAMP/PKA dependent manner
Whalen et al., Hum Exp Toxicol 2006 : These results indicate that AC inhibition caused alterations of the levels of key enzymes, while decreased PKA activity caused an increase in p44/42 activation ... These results indicate that AC inhibition caused alterations of the levels of key enzymes, while decreased PKA activity caused an increase in p44/42 activation
Rubis et al., Mol Cell Endocrinol 2006 (Second Messenger Systems) : In the present study we show that HSD11B2 expression in human renal epithelial P58 cells is regulated at the mRNA and protein level, and that protein kinases A (PKA) and C ( PKC ) are involved in this process
Queen et al., J Physiol 2006 : beta ( 2 ) AR activation also increased NOS activity and phosphorylation of NOS-3 on serine-1177, and these increases were attenuated by inhibition of protein kinase A (PKA) , phosphatidylinositol 3-kinase (PI3K) or Akt, and abolished by coinhibition of PKA and Akt ... beta ( 2 ) AR activation also increased NOS activity and phosphorylation of NOS-3 on serine-1177, and these increases were attenuated by inhibition of protein kinase A (PKA) , phosphatidylinositol 3-kinase (PI3K) or Akt, and abolished by coinhibition of PKA and Akt
Costes et al., Diabetes 2006 : This indicates that ERK1/2, reported to be located downstream of PKA, participates in the PKA mediated CREB phosphorylation elicited by glucose
Huang et al., Am J Physiol Cell Physiol 2007 : RGS4 phosphorylation and inhibition of PI hydrolysis were blocked by selective PKA and PKG inhibitors
Perez et al., J Neurophysiol 2006 (Ion Channel Gating) : However, the D ( 2 ) R effects on inward rectification and RMP were blocked by inhibition of PI-PLC, but not PKA activity
Calebiro et al., Mol Endocrinol 2006 : The inhibitory effect of PKA I apparently required constitutive MAPK activation and was associated with an inhibition of ERK phosphorylation
Kara et al., Mol Endocrinol 2006 : Galpha ( s ) /PKA dependent ERK activation was rapid, transient, and blocked by H89 ( a PKA inhibitor ), but it was insensitive to small interfering RNA mediated depletion of beta-arrestins
Carmosino et al., Biol Cell 2007 : Phosphorylation experiments, combined with immunolocalization studies, indicated that AQP4 phosphorylation is mediated by PKA and occurs subsequently to its internalization in late endosomes
Siarey et al., J Neurochem 2006 (Down Syndrome) : Here we report that calcium/calmodulin dependent protein kinase II ( CaMKII ), phosphatidylinositol 3-kinase (PI3K)/Akt, extracellular signal regulated kinase ( ERK ), protein kinase A (PKA) and protein kinase C ( PKC ), all of which have been shown to be involved in synaptic plasticity, are altered in the Ts65Dn hippocampus
Hsieh et al., Am J Physiol Endocrinol Metab 2007 (Anorexia) : To determine whether PKA or CREB was involved in the anorectic response, intracerebroventricular infusions of antisense oligonucleotide ( or missense control ) were performed 60 min before daily AMPH treatment in conscious rats, and results showed that either PKA or CREB knockdown could block AMPH induced anorexia as well as restore NPY mRNA level, indicating the respective involvement of PKA and CREB signaling in the regulation of NPY gene expression ... It is suggested that hypothalamic PKA and CREB signaling may involve the central regulation of AMPH mediated feeding suppression via the modulation of NPY gene expression
Yong et al., J Biol Chem 2006 : However, unlike the other known MYPTs, which upon phosphorylation inhibit PP1c, PKA phosphorylation of MYPT3 resulted in PP1c activation, indicating a different mode of action
Jensen et al., Cell Signal 2007 : The PKA inhibitor H89 ( 50 microM ) reduced adrenaline stimulated GSK-3beta Ser9 phosphorylation but did not influence the effects of insulin
Yang et al., Cancer Res 2006 : Inhibition of PKA by specific PKA inhibitor H89 or by PKA inhibitor peptide blocked STAT3 activation and suppressed TGF-beta1 induced apoptosis and EMT
Urschel et al., J Biol Chem 2006 : To clarify this experimental discrepancy, we examined protein kinase A (PKA) induced phosphorylation of Cx36 in mouse retina as a possible mechanism to modulate the extent of gap junctional coupling ... Furthermore, activation of PKA increased the level of phosphorylation of Cx36 ... cAMP stimulated, PKA mediated phosphorylation of Cx36 protein was accompanied by a decrease of tracer coupling between AII amacrine cells
Lee et al., J Cell Biol 2006 (Synaptic Transmission) : PKA activated ApAF-ApC/EBP heterodimer is a key downstream effector of ApCREB and is necessary and sufficient for the consolidation of long-term facilitation ... PKA activated ApAF-ApC/EBP heterodimer is a key downstream effector of ApCREB and is necessary and sufficient for the consolidation of long-term facilitation ... Collectively, these results suggest that PKA activated ApAF-ApC/EBP heterodimer is a core downstream effector of ApCREB in the consolidation of LTF ... Collectively, these results suggest that PKA activated ApAF-ApC/EBP heterodimer is a core downstream effector of ApCREB in the consolidation of LTF
Wang et al., Oncol Rep 2006 : AKAPs competing peptide HT31 disrupts the inhibitory effect of PKA on RhoA activity ... The results showed that HT31 not only blocked the PKA induced phosphorylation of RhoA but also prevented the PKA induced inhibition on RhoA activation
Lin et al., J Ethnopharmacol 2007 : Furthermore, LC-induced CREB phosphorylation and protective effect could be blocked by a PKA inhibitor and overexpression of the dominant negative CREB, respectively
Moniri et al., Neurosci Lett 2006 : Results for cis-PAB suggest H1/PLC/IP/DAG/PKC signaling activates PKA , downstream of cAMP formation, indicating apparent direct activation of PKA by PKC
Launay et al., Exp Cell Res 2006 : Upstream of p38, we found that RhoK, PKC and PKA are selectively involved in the activation of p38 and phosphorylation of alphaB-crystallin , depending on the cytoskeletal network disorganized ... Upstream of p38, we found that RhoK, PKC and PKA are selectively involved in the activation of p38 and phosphorylation of alphaB-crystallin, depending on the cytoskeletal network disorganized
McQuaid et al., J Endocrinol 2006 (Obesity) : However, there was no effect of ucp2-OE on cAMP concentrations or PKA activity
Moir et al., Proc Natl Acad Sci U S A 2006 : These results indicate that negative regulation of Maf1 by PKA is achieved by inhibiting its nuclear import and suggest that a PKA independent activation step is required for nuclear Maf1 to function in the repression of pol III transcription
Madhyastha et al., Biochim Biophys Acta 2006 : C-pc induced uPA expression was completely inhibited by PKA inhibitor ( KT 5200 ), indicating the regulation is dependent on PKA pathway
Rui et al., J Neurosci 2006 : However, stimulation of protein kinase A (PKA) by forskolin, cAMP analogs, or neuropeptides effectively alleviated the impairment
Li et al., Circ Res 2006 : Increasing PKA activity, by cell dialysis with cAMP, or inhibition of PKA did not affect I(K1) in WT cells
De Gregorio et al., Oncogene 2007 : We suggest that ( 1 ) TSH-cAMP induced PKA phosphorylates p85alpha ( PI3K ) at serine 83, ( 2 ) phosphorylated p85alpha ( PI3K ) binds RIIbeta-PKA and targets PKAII to the membrane, and ( 3 ) PI3K activity and p21Ras binding to PI3K increase and activate PI3K downstream targets
Kim et al., Mol Endocrinol 2007 : This study demonstrates that the activation of RankL gene expression by PKA- and gp130-inducers is mediated via common regulatory domains that also served to facilitate the activity of 1,25- ( OH ) 2D3
Sosroseno et al., Oral Microbiol Immunol 2006 : The effect of cAMP analog or forskolin on arginase activity in the presence or absence of A. actinomycetemcomitans LPS was blocked by the PKA inhibitor ( KT5720 )
Al-Wadei et al., Cancer Detect Prev 2006 (Adenocarcinoma...) : In BEAS-2B and NCI-H69 cells, the stimulation of cAMP/PKA reduced the phosphorylation of ERK1/2 and inhibited cell proliferation whereas phosphorylation of ERK1/2 and cell proliferation were increased in HPL1D and NCI-H322 cells ... In BEAS-2B and NCI-H69 cells, the stimulation of cAMP/PKA reduced the phosphorylation of ERK1/2 and inhibited cell proliferation whereas phosphorylation of ERK1/2 and cell proliferation were increased in HPL1D and NCI-H322 cells
Li et al., Mol Endocrinol 2007 (Colorectal Neoplasms) : The small GTPase Rap1 can be activated by both PKA and Epac ; however, the role of Rap1 in hormone secretion is unknown ... PKA activation increased NT release and Rap1 activity
Yokoyama et al., J Clin Invest 2006 : Activation of EP4, adenylyl cyclase, and PKA all increased HA production and the level of HA synthase 2 (HAS2) transcripts
Saha et al., Pigment Cell Res 2006 : Moreover, we also show that activation of p38 mediated by lipids is potentiated by inhibition of the PI3kinase pathway but not by inhibition of protein kinase A (PKA)
Rossi et al., Endocrinology 2007 : Finally, we demonstrate that FSH protects Sertoli cells against the pro-apoptotic activity of AEA, through PKA and aromatase dependent activation of FAAH
Stefan et al., J Am Soc Nephrol 2007 : In primary cultured principal cells, AVP causes focal activation of PKA
Chedid et al., J Immunol 1991 (Thymoma) : However, the induction of AP-1 activity by IL-1 and phorbol esters is dependent upon the presence of PKA , as evidenced by the loss of AP-1 inducibility in cells transfected with a cDNA encoding protein kinase inhibitor, a specific inhibitor of PKA
Bolon et al., J Cell Physiol 2007 : We propose that LPS reduces inter-endothelial electrical coupling via tyrosine-, ERK1/2-, PKA- , and PKC dependent signaling that targets Cx40
Ponsioen et al., Exp Cell Res 2007 : This degree of cAMP transfer sufficed to evoke a well characterized response to cAMP in neighbor cells, i.e. the PKA mediated phosphorylation of the ER transcription factor in A431 carcinoma cells
Misra et al., J Cell Biochem 2007 : The PKA inhibitor H-89, greatly attenuated forskolin induced Akt1 synthesis
Gardner et al., J Biol Chem 2007 : Thus, the PDZ and its associated SAP97-AKAP79 complex are involved in targeting the cyclic AMP dependent protein kinase (PKA) to the beta ( 1 ) -AR ... Thus, the PDZ and its associated SAP97-AKAP79 complex are involved in targeting the cyclic AMP dependent protein kinase (PKA) to the beta ( 1 ) -AR
Gu et al., J Appl Physiol 2007 : Furthermore, pretreatment with SQ 22536 ( 100-300 microM, 15 min ), an adenylate cyclase inhibitor, and H89 ( 3 microM, 15 min ), a PKA inhibitor , completely abolished the potentiating effect of epinephrine
Yu et al., J Neurosci Res 2007 : Together, the data suggest that MAPK and PKA regulate rhodopsin transcription through parallel signal transduction pathways
Granneman et al., J Biol Chem 2007 : Double label immunofluorescence analysis of 3T3-L1 adipocytes indicated that PKA activation increases the translocation of hormonesensitive lipase (HSL) to perilipin A (Plin) containing droplets and increases the colocalization of adipose tissue triglyceride lipase ( Atgl ) with its coactivator, Abhd5
Ziegler et al., J Biol Chem 1991 : We have utilized the tumor promoter 12-O-tetradecanoylphorbol 13-acetate ( TPA ) to down-regulate PKC, in order to test for an effect on the PKA mediated induction of the uPA gene expression
Regimbald-Dumas et al., J Cell Biochem 2007 : The purpose of this study was to investigate the effect of cAMP dependent protein kinase (PKA) on the activity of IP(3)R-2 in AR4-2J cells ... Our results are consistent with an enhancing effect of PKA on IP(3)R-2 activity
Gorostizaga et al., Mol Cell Endocrinol 2007 : In adrenocortical and Leydig cells PKA activation by trophic hormones increases the activity of protein tyrosine phosphatases and also induces the expression of MAP kinase phosphatase 1 (MKP-1) , a dual activity protein phosphatase ( serine/threonine and tyrosine )
Levallet et al., Biol Reprod 2007 : Resensitization of the cAMP response to FSH in 20-day-old Sertoli cells was also associated with the highest FSH induced transient increase in both soluble and particulate PDE4 activities, which suggests developmental changes in the PKA mediated upregulation of the catalytic activities of long PDE4D
Tu et al., Mol Cell Proteomics 2007 (Neoplasms, Hormone-Dependent...) : We also determined that serine 330 and threonine 366 of NDRG1 were phosphorylated and demonstrated that the phosphorylation of NDRG1 was prominently mediated by protein kinase A (PKA)
Park et al., Biochem Biophys Res Commun 2007 : PKA , ERK, and p38 MAPK pathways regulate COX-2 mRNA expression induced by rolipram, in which PKA is a central regulator of the ERK and p38 MAPK pathways
Gomes et al., Traffic 2007 : GluR4 delivery to synapses in rat hippocampal slices was shown to require protein kinase A (PKA) mediated phosphorylation of GluR4 at serine 842 ( Ser842 )
Obara et al., J Neurochem 2007 : We show that both Ras and Rap1 can be activated by cAMP or PACAP via protein kinase (PKA) dependent mechanisms ... We show that both Ras and Rap1 can be activated by cAMP or PACAP via protein kinase (PKA) dependent mechanisms
Soulsby et al., Cell Calcium 2007 : Several studies have shown that PKA mediated phosphorylation of IP3R1 at serines S1588 and S1755 enhances the receptor 's ability to mobilize Ca2+ ... IP3R3, however, is known to be phosphorylated by PKA at three sites ( S916, S934, and S1832 ) and, thus, we examined how phosphorylation of these sites affects Ca2+ mobilization in DT40-3KO cells stably expressing either exogenous wild-type or mutant IP3R3s ; an antibody raised against phospho-serine 934 of IP3R3 was used to demonstrate that the exogenous IP3R3s are strongly phosphorylated in response to PKA activation
Bobrovskaya et al., Cell Signal 2007 : PACAP caused sustained activation of PKA , but did not sustain activation of other protein kinases including ERK, p38 kinase, PKC, MAPKAPK2 and MSK1
Ansari et al., Carcinogenesis 2007 : A protein kinase A (PKA) inhibitor reduced PGE2 mediated CREB phosphorylation in keratinocytes from EP2 transgenic mice
Hall et al., Biochemistry 2007 : Here we demonstrate that the A-kinase anchor protein 150 ( AKAP150 ) is critical for PKA mediated regulation of Cav1.2 in the brain
Shi et al., Growth Horm IGF Res 2007 (Adrenal Cortex Neoplasms...) : Moreover, PKA inhibitors increased IGFBP-2 expression in NCI-H295R adrenocortical cells, and anti-IGFBP-2 antibody reduced their proliferation ... This is the first evidence for PKA dependent regulation of IGFBP-2 expression in adrenocortical cells
Choi et al., Am J Physiol Gastrointest Liver Physiol 2007 : We discovered that the upregulation of CCK transcription involves ERK1/2, PKA , and calmodulin dependent protein kinase mediated pathways
Yamamoto et al., J Biol Chem 2007 : Activation of PKA repressed LXR induced SREBP-1c expression both in rat primary hepatocytes and mouse livers ... Promoter analyses revealed that the LXRalpha binding site in the SREBP-1c promoter is responsible for PKA inhibitory effect on SREBP-1c transcription
Keller et al., J Virol 2007 : The effect is immediate, independent of de novo protein synthesis, associated with the phosphorylation of ATF-1 serine 63 and CREB serine 133, dependent on protein kinase A (PKA) and the enhancer 's CRE, and linked to viral-lytic-cycle advancement
Ahn et al., Proc Natl Acad Sci U S A 2007 : The B56delta subunit is phosphorylated by PKA , and this increases the overall activity of PP2A in vitro and in vivo
Hirano et al., Eur J Pharmacol 2007 : The synergistic production of MCP-1 and IL-8 by PGD2 was mimicked by dibutyryl cAMP ( db-cAMP ) and was inhibited by a protein kinase A (PKA) inhibitor ... The synergistic production of MCP-1 and IL-8 by PGD2 was mimicked by dibutyryl cAMP ( db-cAMP ) and was inhibited by a protein kinase A (PKA) inhibitor
Wang et al., Am J Physiol Endocrinol Metab 2007 : Since our recent studies have shown that PACAP can induce GH secretion in carp pituitary cells through cAMP/PKA- and Ca2+/calmodulin dependent mechanisms, these results, taken together, suggest that alpha2-adrenergic stimulation in the carp pituitary may inhibit PACAP induced GH release and GH gene transcription by blocking the AC/cAMP/PKA pathway and Ca2+ entry through L-type VSCC
D'Angiolella et al., PloS one 2007 : MPF down-regulation required Ca ( ++ ) /calmodulin dependent kinase II ( CaMKII ) and cAMP dependent protein kinase (PKA) activities at meiosis and mitosis exit, respectively
Cardone et al., Mol Biol Cell 2007 (Anoxia...) : The NHERF1 PDZ2 domain regulates PKA-RhoA-p38 mediated NHE1 activation and invasion in breast tumor cells
Simeone et al., Int J Oncol 2007 (Breast Neoplasms...) : Protein kinase A (PKA) and protein kinase C ( PKC ) are activated by COX-2 and are involved in IL-8 regulation ... Protein kinase A (PKA) and protein kinase C ( PKC ) are activated by COX-2 and are involved in IL-8 regulation ... Activation of PKC, not PKA , increased IL-8 production and invasion in MCF-7 cells
Hirakawa et al., J Pharmacol Exp Ther 2007 : These results indicate that LPA induces cAMP/PKA-sensitive , RhoA mediated random migration of BTSMCs. Regulation of this mechanism would be beneficial for the control of airway remodeling
Kuroda et al., Proc Natl Acad Sci U S A 2007 : All observations are consistent with the effects of BIG1 and BIG2 phosphorylation on vesicular trafficking, via alterations in ARF activation and regulatory roles for cAMP, PKA , and PP1gamma in ARF activation by BIG1 and BIG2
Gervasi et al., J Neurosci 2007 : 5-HT7 mediated PKA responses were obtained in 30 s at the membrane, in 2.5 min in the cytosol, and in 13 min in the nucleus
Chaloux et al., Biol Cell 2007 : The purpose of the present study was to investigate the effect of PKA ( protein kinase A ) on the activity of IP(3)R-3 in RINm5F cells ... The PKA induced enhancement of IP(3)R-3 activity was also observed in intact RINm5F cells stimulated with carbachol and epidermal growth factor, two agonists that use different receptor types to activate phospholipase C
Tiecke et al., Dev Biol 2007 : Expressing dominant negative PKA also induced extra, sometimes multiple digits, from anterior limb bud demonstrating the negative role in Shh signaling
Hu et al., J Endocrinol 1992 (Second Messenger Systems) : These data provide direct evidence for a role for both PKC- and PKA mediated mechanisms in the regulation of CRF , AVP and oxytocin release and for differential effects of both glucocorticoids and mineralocorticoids on PKA- and PKC stimulated responses ... These data provide direct evidence for a role for both PKC- and PKA mediated mechanisms in the regulation of CRF, AVP and oxytocin release and for differential effects of both glucocorticoids and mineralocorticoids on PKA- and PKC stimulated responses
Hsieh et al., Am J Pathol 2007 (Hemorrhage...) : We found that suppression of GPR30 but not ER-alpha prevented E2-BSA- or E2-induced PKA activation and Bcl-2 expression ... We found that suppression of GPR30 but not ER-alpha prevented E2-BSA- or E2-induced PKA activation and Bcl-2 expression
Srisawang et al., Biochim Biophys Acta 2007 : In contrast, activation of PKA by both forskolin and epidermal growth factor (EGF) had no effect on succinate transport
Souza et al., J Lipid Res 2007 : NE-induced lipolysis is mediated by protein kinase A (PKA) dependent phosphorylation of perilipin , a lipid droplet associated protein that is the major regulator of lipolysis ... Thus, in the presence of perilipin, PKA mediated perilipin phosphorylation is essential for NE-dependent lipolysis and full adaptive thermogenesis in BAT
Datta et al., J Neurophysiol 2007 : Activation of pedunculopontine tegmental PKA prevents GABAB receptor activation mediated rapid eye movement sleep suppression in the freely moving rat ... These results provide evidence that the activation of cAMP-PKA within the PPT can successfully block the GABA(B) receptor activation mediated REM sleep suppression effect
Kocinsky et al., American journal of physiology. Renal physiology 2007 : To this end, we directly compared PKA induced NHE3 inhibition with site-specific changes in NHE3 phosphorylation in vivo and in vitro ... We conclude that although phosphorylation of NHE3 at serines 552 and 605 is regulated by PKA both in vivo and in vitro, phosphorylation of these sites does not directly alter Na ( + ) /H ( + ) exchange activity
Xie et al., Mol Pharmacol 2007 (MAP Kinase Signaling System...) : Furthermore, PKA dependent Rap1 activation is required for both ERK1/2 activation and androgen independent AR activation in LNCaP cells upon VIP stimulation ... Furthermore, PKA dependent Rap1 activation is required for both ERK1/2 activation and androgen independent AR activation in LNCaP cells upon VIP stimulation
Rey et al., Bone 2007 (MAP Kinase Signaling System) : Activation of p38 is cAMP-PKA dependent and mediates PTH induced stimulation of ALP which plays a critical role for the calcification of the bone matrix ... Activation of p38 is cAMP-PKA dependent and mediates PTH induced stimulation of ALP which plays a critical role for the calcification of the bone matrix
Ohtsubo et al., Arterioscler Thromb Vasc Biol 2007 (Disease Models, Animal) : The induction of ICER was inhibited by pretreatment with H89, a protein kinase A (PKA) inhibitor, suggesting that PKA mediates the induction of ICER expression
Jeon et al., Mol Cells 2007 : These results indicate that PKA phosphorylates CREB and so induces VEGF gene expression
Zhang et al., J Neurosci Res 2007 (Ion Channel Gating...) : Application of a cAMP analog mimicked the effects of GnRH, and only inhibitors of PKA and PKG could prevent GnRH induced inhibition of INa
Sirotkin et al., Physiol Res 2008 : The aim of our in vitro studies was to understand the role of leptin in controlling proliferation, apoptosis, and protein kinase A (PKA) in human ovarian cells ... We analyzed the in vitro effects of leptin ( 0, 1, 10 or 100 ng/ml ) on the accumulation of proliferation related peptides ( PCNA, cyclin B1 ), apoptosis associated peptide ( Bax ) and the intracellular signaling molecule PKA in cultured human granulosa cells using immunocytochemistry and Western immunoblotting ... It was observed that leptin stimulated in a dose dependent manner the accumulation of PCNA ( at doses 1-100 ng/ml ), cyclin B1 ( at doses 10 or 100 ng/ml ), Bax ( at doses 10 or 100 ng/ml ) and PKA ( at doses 1-100 ng/ml ) in cultured human ovarian cells
Shelly et al., Cell 2007 : Finally, local exposure of the undifferentiated neurite to brain derived neurotrophic factor or dibutyryl-cAMP promoted axon differentiation in a manner that depended on PKA dependent LKB1 phosphorylation ... Thus local LKB1/STRAD accumulation and PKA dependent LKB1 phosphorylation represents an early signal for axon initiation
Winters et al., Mol Cell Endocrinol 2007 (Second Messenger Systems) : The MEK inhibitor PD98059 blocked activation by GnRH or forskolin implying that MAPK contributes to cAMP/PKA mediated activation of follistatin
Etkovitz et al., Biol Reprod 2007 : Activation of protein kinase A (PKA) by dibutyryl cAMP enhanced PIP ( 2 ( 4,5 ) ), PIP ( 3 ( 3,4,5 ) ), and F-actin formation, and these effects were mediated through PI3K ... Activation of protein kinase A (PKA) by dibutyryl cAMP enhanced PIP ( 2 ( 4,5 ) ), PIP ( 3 ( 3,4,5 ) ), and F-actin formation, and these effects were mediated through PI3K ... These results suggest that two alternative pathways lead to PI4K activation : indirect activation by PKA , which is mediated by PI3K ; and activation by PKC, which is independent of PI3K activity
Xu et al., J Neurochem 2007 : H89, a protein kinase A (PKA) inhibitor attenuated the PACAP response
Li et al., Shock 2007 : CCK-8 inhibits LPS induced IL-1beta production in pulmonary interstitial macrophages by modulating PKA , p38, and NF-kappaB pathway
Markhotina et al., IET Nanobiotechnol 2007 : We confirmed the activation of PKA by PACAP in experiments where H89 also inhibited the PACAP induced relaxation
Szentandrássy et al., J Physiol 2007 (Calcium Signaling) : Several different approaches indicated activation of PKA by EPA ( 5-10 micromol l ( -1 ) ) : the time constant of decay of the systolic Ca2+ transient decreased to 65.3 +/- 5.0 % of control, Western blot analysis showed a fourfold increase in phospholamban phosphorylation, and PKA activity increased by 21.0 +/- 7.3 %
Kawamura et al., Arterioscler Thromb Vasc Biol 2007 (Atherosclerosis) : In addition, inhibitors of adenylyl cyclase and protein kinase A (PKA) restored IL-1beta signaling in apoE treated VSMCs, whereas apoE stimulated PKA activity ... In addition, inhibitors of adenylyl cyclase and protein kinase A (PKA) restored IL-1beta signaling in apoE treated VSMCs, whereas apoE stimulated PKA activity
Scherer et al., Naunyn Schmiedebergs Arch Pharmacol 2007 : Activation of inwardly rectifying Kir2.x potassium channels by beta 3-adrenoceptors is mediated via different signaling pathways with a predominant role of PKC for Kir2.1 and of PKA for Kir2.2
Nieto et al., J Mol Biol 2007 : Here we demonstrate that, in vitro, Ser101 is phosphorylated by protein kinase A (PKA) , either purified or contained in melanoma ( 624 mel ) nuclear extract, and that this phosphorylation indeed significantly reduced N Oct-3 DBD binding to PORE and NORE motifs, most likely by hampering the POUh rigid arm insertion in the DNA minor groove
Lin et al., J Cell Biol 2007 : Among actin associated proteins, Enabled ( Ena ) /vasodilator stimulated phosphoprotein ( VASP ) were predicted as PKA effectors downstream of syndecan-2, as Ena/VASP , which is activated by PKA , induces actin polymerization ... This study reveals a novel signaling pathway in which syndecan-2 activates PKA via neurofibromin and PKA consequently phosphorylates Ena/VASP, promoting filopodia and spine formation
Ren et al., Eukaryotic cell 2007 : These dominant mutations alleviate the temperature-sensitive growth defect by elevating PKA activity, as judged by their effects on PKA regulated processes, localization and phosphorylation of the PKA effector Msn2 , as well as in vitro PKA activity
Borthwick et al., Mol Biol Cell 2007 (Ion Channel Gating) : Analysis of 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid ( DIDS ) and CFTR ( inh172 ) -sensitive currents, taken as indication of the outwardly rectifying Cl ( - ) channels ( ORCC ) and CFTR mediated currents, respectively, showed that Ac1-14, but not N1-14, inhibits both the cAMP/PKA dependent ORCC and CFTR activities
Bulun et al., J Steroid Biochem Mol Biol 2007 (Breast Neoplasms...) : In breast adipose fibroblasts exposed to PGE ( 2 ) secreted by malignant epithelial cells, PKC is also activated, and this potentiates cAMP-PKA dependent induction of aromatase
Harada et al., Biol Reprod 2007 : The MEK inhibitor, U0126, and the PKA inhibitors, H89 and cAMP dependent protein kinase peptide inhibitor ( PKI ), completely inhibited MAPK3/1 activation by either ADCYAP1 or CPT-cAMP ... The MEK inhibitor, U0126, and the PKA inhibitors, H89 and cAMP dependent protein kinase peptide inhibitor ( PKI ), completely inhibited MAPK3/1 activation by either ADCYAP1 or CPT-cAMP ... These results suggest that in LbetaT2 cells, ADCYAP1 mainly increases Cga through activation of PKA and MAPK3/1, as well as through an additional unknown pathway
Levin et al., ACS Chem Biol 2007 : Examined here, the caveolin-1 scaffolding domain ( CSD ) binds to and inhibits both endothelial nitric oxide synthase (eNOS) and protein kinase A (PKA)
Sirotkin et al., Comp Biochem Physiol A Mol Integr Physiol 2007 : Finally, leptin inhibited expression of MAPK/ERK1,2 and CDK/p34 and stimulated expression of PKA
Zwart et al., EMBO J 2007 : In a previous study, we showed that protein kinase A (PKA) mediated phosphorylation of serine 305 ( S305 ) of ERalpha results in resistance to tamoxifen ... This intermolecular reorientation as a result of PKA mediated phosphorylation of ERalpha-S305 and tamoxifen binding provides a unique model for resistance to the anticancer drug tamoxifen
Burelout et al., Biochem Pharmacol 2007 : These data provide evidence that PGE2 stimulated PKA activity regulates the PLD pathway stimulated by fMLP at the level of PI3-Kgamma and that the inhibition of PI3-Kgamma activation by PKA is a complex mechanism that remains to be completely elucidated
Wei et al., Biochem Pharmacol 2007 (Adenocarcinoma...) : Activation of ERK and p38 MAPK appeared to be mediated primarily by transactivation of EGFR, whereas activation of CREB and NF-kappaB was mediated by PKA , PKC and ERK ... Activation of ERK and p38 MAPK appeared to be mediated primarily by transactivation of EGFR, whereas activation of CREB and NF-kappaB was mediated by PKA , PKC and ERK
Francis et al., Am J Physiol Cell Physiol 2007 (Bile Duct Diseases) : In BDL rats, UK 14,304 inhibited secretin stimulated 1 ) bile and bicarbonate secretion, 2 ) expansion of IBDU luminal spaces, and 3 ) cAMP levels, PKA activity, Cl ( - ) efflux, and Cl ( - ) /HCO ( 3 ) ( - ) exchanger activity in cholangiocytes
Sun et al., Pharm Res 2008 : The protein kinase A (PKA) inhibitors ( H89 and KT5720 ), but not a PKG inhibitor, blocked the polarized membrane expression of OATPC-GFP and reduced estrone-3-sulfate transport activity
Lee et al., Biochem Pharmacol 2007 (Melanoma) : In addition, after the melanin content was increased by rosmarinic acid, it was reduced by H-89 and KT 5720, protein kinase A (PKA) inhibitors , but not by SB203580, a p38 ( mapk ) inhibitor, or Ro-32-0432, a PKC inhibitor, which suggests the involvement of PKA in rosmarinic acid induced melanogenesis
Kockx et al., Circ Res 2007 (Calcium Signaling) : We investigated the roles of the actin skeleton, microtubules, protein kinase A (PKA) and calcium ( Ca2+ ) in regulating apoE secretion from macrophages
Toffoli et al., Biochim Biophys Acta 2007 : Intermittent hypoxia changes HIF-1alpha phosphorylation pattern in endothelial cells : unravelling of a new PKA dependent regulation of HIF-1alpha
Juric et al., Neurochem Int 2008 : BDNF levels in astrocytes were increased by the specific beta1-adrenergic agonist dobutamine and the beta2-adrenergic agonist salbutamol, as well as by adenylate cyclase activation ( by forskolin ) and PKA activation ( by dBcAMP )
Hsieh et al., Physiol Genomics 2007 (Anorexia...) : Aims of this study were to investigate whether protein kinase A (PKA) signaling is involved in regulating NPY gene expression and PPA induced anorexia ... Results also showed that PKA knock-down could block PPA induced anorexia as well as restore NPY mRNA level, indicating the involvement of PKA signaling in the regulation of NPY gene expression
Eckert et al., J Leukoc Biol 2007 : Our hypothesis is that PKA regulates neutrophil migration through VASP Ser 157 phosphorylation ... These data show that chemoattractant stimulation of human neutrophils induces a rapid and transient PKA dependent VASP Ser 157 phosphorylation
Luo et al., J Gene Med 2007 (Carcinoma) : The PKA and PI3K signal transduction pathways are likely involved in enhanced Cx43 expression as inhibitors of these pathways prevented Cx43 upregulation
Borgkvist et al., Neuropharmacology 2008 : These results indicate that, in the striatum, THC promotes PKA dependent phosphorylation of DARPP-32 in striatal medium spiny neurons expressing dopamine D1 and adenosine A2A receptors
Joy et al., Am J Respir Cell Mol Biol 2008 : The iodide efflux seen with 8-iso-PGE ( 2 ) is abolished by the EP ( 4 ) receptor antagonist AH23848, the CFTR inhibitor 172, and inhibition of PKA and the PI3K pathway
Dash et al., Learn Mem 2007 (Memory Disorders) : Additionally, the negative influence of calcium- and cAMP dependent protein kinase ( i.e., calcium/calmodulin dependent protein kinase II ( CaMKII ), calcium/diacylglycerol activated protein kinase C ( PKC ), and cAMP dependent protein kinase A (PKA) ) activities on working memory will be reviewed
Xiao et al., J Biol Chem 2007 : Importantly, the S2030A mutation, but not mutations of Ser-2,808, diminished the effect of PKA on RyR2
Soodvilai et al., American journal of physiology. Renal physiology 2007 : These data suggest that H2O2 stimulates CFTR Cl ( - ) channel mediated Cl ( - ) secretion through cyclooxygenase- and mPGES-1 dependent release of PGE2 and subsequent activation of PKA
Ferris et al., Biol Reprod 2007 : In LbetaT2 cells, cAMP stimulation of the Lhb promoter was eliminated by inhibition of protein kinase A (PKA) ; GnRH1 stimulation was partially suppressed by either PKA or protein kinase C inhibitors ... Thus, cAMP stimulation of Lhb promoter activity, and enhancement of GnRH1 stimulation, occurs in multiple physiological states independent of steroid status, via a PKA dependent mechanism ... Thus, cAMP stimulation of Lhb promoter activity, and enhancement of GnRH1 stimulation, occurs in multiple physiological states independent of steroid status, via a PKA dependent mechanism
O'Driscoll et al., J Neurochem 2007 : The effects of bFGF on GSK3beta were dependent on protein kinase A (PKA) activation, as inhibition of this pathway blocked inactivation ... Activation of the transcription factor cAMP-response element binding protein ( CREB ) and subsequent up-regulation of Bcl-2 in response to bFGF was also dependent on PKA as inhibition with H-89 attenuated increased pCREB levels and Bcl-2 expression ... Activation of the transcription factor cAMP-response element binding protein ( CREB ) and subsequent up-regulation of Bcl-2 in response to bFGF was also dependent on PKA as inhibition with H-89 attenuated increased pCREB levels and Bcl-2 expression ... Activation of the transcription factor cAMP-response element binding protein ( CREB ) and subsequent up-regulation of Bcl-2 in response to bFGF was also dependent on PKA as inhibition with H-89 attenuated increased pCREB levels and Bcl-2 expression ... These results indicate that the protective efficacy of bFGF in mouse photoreceptors involves PKA dependent inactivation of GSK3beta and subsequent up-regulation of Bcl-2 via CREB activation
Vetter et al., Eur J Pain 2008 (Ion Channel Gating) : Since the mu opioid receptor (MOP) agonist morphine can inhibit TRPV1 responses potentiated by cAMP dependent protein kinase A (PKA), and ethanol mediated modulation of other ion channels involves activation of PKA , we aimed to assess the contribution of MOP-sensitive pathways to the potentiation of TRPV1 mediated capsaicin responses by ethanol
Chemin et al., J Biol Chem 2007 : In this study, we demonstrate that protein kinase A (PKA) and PKC ( but not PKG ) activation induces a potent increase in Ca(V)3.1 , Ca(V)3.2, and Ca(V)3.3 currents in various mammalian cell lines
Marcet-Palacios et al., Biochem Biophys Res Commun 2007 : PKA dependent WT1 phosphorylation results in translocation of WT1 from the nucleus to the cytosol, a process that interferes with WT1 transcriptional activities
Chen et al., Cancer Res 2007 (Breast Neoplasms) : Specific activation of protein kinase A (PKA) or EPAC with cAMP analogues stimulated p38 and JNK1 ; however, only PKA activating cAMP analogues induced aromatase expression ... Taken together, PGE ( 2 ) activation of p38 and JNK1 via PKA and PKC is necessary for aromatase induction in BAFs, and p38 and JNK1 are potential new drug targets for tissue-specific ablation of aromatase expression in breast cancer ... Taken together, PGE ( 2 ) activation of p38 and JNK1 via PKA and PKC is necessary for aromatase induction in BAFs, and p38 and JNK1 are potential new drug targets for tissue-specific ablation of aromatase expression in breast cancer
Wales et al., J Neurochem 2007 : In one clone ( PC47 ), ICP10PK inhibited caspase-3 activation through up-regulation/stabilization of adenylate cyclase ( AC ) , activation of PKA and MEK, and the convergence of the two pathways on extracellular signal regulated kinase activation
McConville et al., Am J Physiol Endocrinol Metab 2007 : These findings are consistent with beta ( 1 ) -AR-specific PKA dependent glycogen phosphorylase kinase signaling
Ruppelt et al., J Immunol 2007 : cAMP negatively regulates T cell immune responses by activation of type I protein kinase A (PKA) , which in turn phosphorylates and activates C-terminal Src kinase (Csk) in T cell lipid rafts
Sun et al., Am J Physiol Gastrointest Liver Physiol 2007 (Pancreatitis) : Prostaglandin E2 modulates TNF-alpha induced MCP-1 synthesis in pancreatic acinar cells in a PKA dependent manner
Baggaley et al., J Biol Chem 2007 : These data suggest that PKA mediated phosphorylation of PMCA1 differentially regulates [ Ca ( 2+ ) ] ( i ) clearance in the apical region of parotid acinar cells because of a dynamic translocation of PKA
Machado et al., J Neurosci Res 2008 (MAP Kinase Signaling System) : Inhibiting calcium/calmodulin dependent kinase II ( CaMKII ), calcineurin, or protein kinase A (PKA) activity prevents KCl induced IPD-IEG mRNA accumulation, suggesting that the IPD-IEG genes are induced by depolarization in neurons via a combination of calcineurin/PKA- and CaMKII dependent pathways
Yang et al., Biochim Biophys Acta 2008 (Ion Channel Gating) : The VIP induced Kir6.1/SUR2B channel activation was blocked by PKA inhibitor RP-cAMP ... The VIP induced Kir6.1/SUR2B channel activation was blocked by PKA inhibitor RP-cAMP
Szatmari et al., J Neurosci 2007 : In cortical neurons, ERK1/2 activation by forskolin was protein kinase A (PKA) dependent but TrkB ( receptor tyrosine kinase B ) independent and was accompanied by the increased association between KSR1 and active ERK1/2 ... In cortical neurons, ERK1/2 activation by forskolin was protein kinase A (PKA) dependent but TrkB ( receptor tyrosine kinase B ) independent and was accompanied by the increased association between KSR1 and active ERK1/2
Haworth et al., J Mol Cell Cardiol 2007 : These results show that ET1 induced PKD activation in ARVM is mediated by PKC, primarily the PKCepsilon isoform, and is suppressed by PKA activation
Jones et al., Biochem J 2008 : To gain insight into the structural basis of the regulation of RyR2 by PKA , we determined the three-dimensional location of the PKA site Ser2030
Lu et al., EMBO J 2007 : Other studies demonstrate that GluR1 homomeric AMPA receptors are the main GluR2 lacking AMPA receptors in adult hippocampus and require PKA for their functional postsynaptic expression during potentiation
Ikari et al., Biochim Biophys Acta 2008 : The activation of CaSR induced a decrease in PKA activity
Park et al., Int J Dev Neurosci 2007 : Preventing protein kinase A (PKA) activation with PKI attenuated expression of DBH in parkin overexpressed LC3541 cells
Rein et al., Am J Physiol Cell Physiol 2008 : Here, we analyze whether the effect of cAMP on V-ATPase is mediated by protein kinase A (PKA) or exchange protein directly activated by cAMP ( Epac ), the cAMP target proteins that are present within the salivary glands
Cieslak et al., Mol Cells 2007 : On the contrary, inhibition of PKA or specifically PKCdelta resulted in up-regulation of BAD
Bloomer et al., J Biol Chem 2008 : A PKA dependent increase in Arc protein level was observed after pharmacological or synaptic activation of N-methyl-D-aspartate ( NMDA ) receptors, which play a critical role in both LTP induction and learning
Etique et al., Int J Oncol 2007 (Breast Neoplasms) : Using PKA inhibitor ( H89 ), the study of phospho-CREB by Western blot and transfection experiments with a CRE-reporter construct demonstrated that PKA was involved in ethanol induced transcription of ERalpha target genes
Shen et al., J Biol Chem 2008 : cAMP/PKA activation and the downstream Rho inhibition are possible mechanisms by which myocilin in overabundance may lead to TM cell or tissue damage
Dyson et al., Biol Reprod 2008 : The expression of the steroidogenic acute regulatory protein ( STAR ) is regulated by PKA in response to trophic hormone stimulation through the second messenger cAMP
Motzkus et al., Chronobiol Int 2007 : Conversely, PKA induced activation of hPER1 was reduced by the inhibition of CREB and the CCAAT-box binding protein C/EBP, but not by AP1
Murthy et al., Am J Physiol Gastrointest Liver Physiol 2008 : We have previously shown that cAMP levels are regulated by PKA mediated phosphorylation of cAMP-specific phosphodiesterase 3A (PDE3A) and PDE4D5 ; the latter is the only PDE4D isoform expressed in smooth muscle ... We have previously shown that cAMP levels are regulated by PKA mediated phosphorylation of cAMP-specific phosphodiesterase 3A (PDE3A) and PDE4D5 ; the latter is the only PDE4D isoform expressed in smooth muscle
Woo et al., Biochem Biophys Res Commun 2008 : These results indicate that, in AQP5, serine156 may be phosphorylated by PKA , but membrane expression of AQP5 may not be regulated by PKA phosphorylation
Hochbaum et al., J Biol Chem 2008 : Since cAMP mediated activation of the G-protein Rap1 and its phosphorylation by PKA are strictly required for the effects of cAMP on mitogenesis, we hypothesized that the Rap1 activator Epac might represent the PKA independent factor
Liang et al., Mol Cell Neurosci 2008 : Lithium inhibits Smad3/4 transactivation via increased CREB activity induced by enhanced PKA and AKT signaling
Murthy et al., Am J Physiol Cell Physiol 2008 : The present study identified the novel role of PKA in homologous desensitization of VPAC(2) receptors via the phosphorylation of GRK2 at Ser ( 685 )
Kim et al., Mol Cell Biol 2008 : The antiapoptotic effect of GIP in beta cells is therefore partially mediated through a novel mode of transcriptional regulation of Bcl-2 involving cAMP/PKA/AMPK dependent regulation of CREB/TORC2 activity ... The antiapoptotic effect of GIP in beta cells is therefore partially mediated through a novel mode of transcriptional regulation of Bcl-2 involving cAMP/PKA/AMPK dependent regulation of CREB/TORC2 activity
Chytrova et al., Eur J Neurosci 2008 (Brain Injuries...) : In agreement, we found that exercise increased PKA levels and this effect was prevented by BDNF blockade
El-Haroun et al., Am J Physiol Lung Cell Mol Physiol 2008 : Fluorescent kemptide assay and Western blotting confirmed that PKA and p38 MAP kinase were activated by IL-1beta, BK, and TGF-beta1 ... Fluorescent kemptide assay and Western blotting confirmed that PKA and p38 MAP kinase were activated by IL-1beta , BK, and TGF-beta1
Pastor-Soler et al., Am J Physiol Cell Physiol 2008 : The PKA inhibitor myristoylated-PKI ( mPKI, 10 microM ) inhibited the apical accumulation of V-ATPase induced by 6-MB-cAMP ... We conclude that direct stimulation of PKA activity by cAMP is necessary and sufficient for the alkaline pH-induced accumulation of V-ATPase in clear cell apical microvilli
Yu et al., Apoptosis 2008 (Myocardial Reperfusion Injury) : Moreover, insulin blunted ISO mediated increase in PKA activity, enhanced the PKA dependent phosphorylation of phospholamban (PLB) , resulting in increased sarcoplasmic reticulum Ca2+-ATPase ( SERCA2a ) activity ... The inhibitory effect of insulin on the beta-adrenergic action involved the inhibition of PKA mediated Ca2+ transient and promotion of post-ischemic Ca2+ handling
Benz et al., Int Immunopharmacol 2008 : Recently, we identified a novel interaction between alpha II-spectrin and vasodilator stimulated phosphoprotein ( VASP ), which is abrogated by the cAMP dependent protein kinase (PKA) mediated phosphorylation of VASP
Dammer et al., J Biol Chem 2008 : Further, we postulated that PKA evokes changes in the phosphorylation status of CtBP1 that control the ability of the protein to bind to steroidogenic factor-1 and the coactivator GCN5 ( general control nonderepressed 5 ) and repress CYP17 gene expression ... We provide evidence that PKA 1 ) induces metabolic changes in the adrenal cortex and 2 ) phosphorylates CtBP proteins, particularly CtBP1 at T144, resulting in CtBP protein partnering and ACTH dependent CYP17 transcription
Borthwick et al., Cell Calcium 2008 : The annexin 2-S100A10 complex and its association with TRPV6 is regulated by cAMP/PKA/CnA in airway and gut epithelia ... The annexin 2-S100A10 complex and its association with TRPV6 is regulated by cAMP/PKA/CnA in airway and gut epithelia ... The annexin 2-S100A10 complex and its association with TRPV6 is regulated by cAMP/PKA/CnA in airway and gut epithelia ... Thus, the cAMP/PKA/CnA induced anx 2-S100A10/TRPV6 complex may require additional factors for calcium influx or play a role independent of calcium influx in airway epithelia ... Thus, the cAMP/PKA/CnA induced anx 2-S100A10/TRPV6 complex may require additional factors for calcium influx or play a role independent of calcium influx in airway epithelia
Li et al., Cell Res 2008 : Moreover, activation of PKA induces serine/threonine phosphorylation, reduces tyrosine phosphorylation of insulin receptor substrate 1 (IRS-1) and the association between PKA and IRS-1
Yao et al., Mol Pharmacol 2008 : Direct activation of PKA by adenosine-3',5'-cyclic monophosphorothioate, Sp-isomer ( Sp-cAMPS ), prostaglandin E1, or the adenosine A2A receptor is sufficient to cause epsilonPKC translocation to the cytosolic compartment in a process that is dependent on PLC activation and requires PKA activity
Marais et al., Cardiovasc Drugs Ther 2008 (Myocardial Reperfusion Injury) : Activation of PKA , PKC, ERK and p38 MAPK is involved in preconditioning induced CREB phosphorylation
Puana et al., Anesth Analg 2008 : Cyclic adenosine monophosphate inhibits the phosphoinositide/MAP kinase hyperpermeability pathway via the protein kinase A (PKA) dependent inhibition of Raf-1
Yang et al., PloS one 2008 : cAMP/PKA regulates osteogenesis, adipogenesis and ratio of RANKL/OPG mRNA expression in mesenchymal stem cells by suppressing leptin ... All these effects are mediated by the cAMP/PKA/CREB pathway by suppressing leptin , and may contribute to PKA stimulators induced in vivo bone loss in developing zebrafish
Park et al., J Leukoc Biol 2008 : Inhibition of either ERK1/2 or PKA activity prevented gAcrp stimulated CREB phosphorylation, as well as gAcrp stimulated IL-10 promoter activation ... Inhibition of either ERK1/2 or PKA activity prevented gAcrp stimulated CREB phosphorylation, as well as gAcrp stimulated IL-10 promoter activation
Du et al., Mol Cell Biol 2008 : Concomitantly, increased intracellular cAMP mediated PKA activation also resulted in an enhanced nuclear accumulation of histone deacetylase 4 (HDAC4) and a subsequent increase in the MEF2D-HDAC4 repressor complex ... Concomitantly, increased intracellular cAMP mediated PKA activation also resulted in an enhanced nuclear accumulation of histone deacetylase 4 (HDAC4) and a subsequent increase in the MEF2D-HDAC4 repressor complex
Seo et al., Exp Mol Med 2008 : Further, we obtained evidence of crosstalk between P2 receptors, in a situation where intracellular Ca ( 2+ ) release and/or cAMP activated PKA were the main contributors to extracellular ATP- ( or ADP ) -mediated IL-10 expression, and IL-10 production was down-regulated by either MRS2179 ( a P2Y ( 1 ) antagonist ) or 5'-AMPS ( a P2Y ( 11 ) antagonist ), indicating that both the P2Y(1) and P2Y(11) receptors are major receptors involved in IL-10 expression ... Further, we obtained evidence of crosstalk between P2 receptors, in a situation where intracellular Ca ( 2+ ) release and/or cAMP activated PKA were the main contributors to extracellular ATP- ( or ADP ) -mediated IL-10 expression, and IL-10 production was down-regulated by either MRS2179 ( a P2Y ( 1 ) antagonist ) or 5'-AMPS ( a P2Y ( 11 ) antagonist ), indicating that both the P2Y(1) and P2Y(11) receptors are major receptors involved in IL-10 expression
Bezerra et al., American journal of physiology. Renal physiology 2008 : In conclusion, long-term exposure to PTH results in reduction of NHE3 mRNA levels due to a PKA dependent inhibitory effect on the NHE3 promoter and a small reduction of mRNA half-life, and decrease in the total amount of protein which is preceded by endocytosis of the apical surface NHE3
Yue et al., Eur J Pharmacol 2008 : The selective PKA inhibitor, H-89, attenuates the sustained morphine mediated augmentation of basal CGRP release, indicating that the cAMP/PKA pathway plays an important role in regulation of CGRP release from sensory neurons
Ohnishi et al., J Neurochem 2008 : These results demonstrate that PACAP stimulated nNOS translocation mediated by PKA and PKC via PAC(1)-receptor ( a PACAP receptor ) and suggest cross-talk between PACAP and NMDA for nNOS activation by Src dependent phosphorylation of NMDA receptors
Arrese et al., Arch Biochem Biophys 2008 : Using lipoprotein complexes containing triolein it is shown that PKA mediated phosphorylation of Lsd1 promoted a 1.7-fold activation of the main fat body lipase demonstrating the direct link between Lsd1 phosphorylation and activation of lipolysis
Borthwick et al., Cell Signal 2008 (Cystic Fibrosis) : Defective formation of PKA/CnA dependent annexin 2-S100A10/CFTR complex in DeltaF508 cystic fibrosis cells ... Defective formation of PKA/CnA dependent annexin 2-S100A10/CFTR complex in DeltaF508 cystic fibrosis cells ... Defective formation of PKA/CnA dependent annexin 2-S100A10/CFTR complex in DeltaF508 cystic fibrosis cells
Taurin et al., Am J Physiol Cell Physiol 2008 : In the present study, using the phosphospecific antibodies against phospho-Ser552 or phospho-Ser675 sites of beta-catenin, we show that ATP can stimulate PKA dependent phosphorylation of endogenous beta-catenin at both of these sites without affecting its expression levels in VSMC ... This translates to a PKA dependent stimulation of TCF transcriptional activity through an increased association of phosphorylated ( by PKA ) beta-catenin with TCF-4 ... Using the PKA inhibitor PKI or dominant negative TCF-4 mutant, we show that ATP induced cyclin D1 promoter activation, cyclin D1 protein expression, and proliferation of VSMC are all dependent on PKA and TCF activities
Seo et al., Arch Pharm Res 2008 : GTS inhibited cAMP levels and protein expression of protein kinase A (PKA)
Demczuk et al., Eukaryotic cell 2008 : Our results suggest that the increased expression of stress-responsive genes in plc1 Delta cells is mediated by decreased cyclic AMP synthesis and protein kinase A (PKA) mediated phosphorylation of Msn2p and increased binding of Msn2p to stress-responsive promoters
Spencer et al., Mol Cell Neurosci 2008 : BDNF activates CaMKIV and PKA in parallel to block MAG mediated inhibition of neurite outgrowth
Yang et al., J Pharmacol Sci 2008 : In our previous study, anti-apoptotic effects of GABA(C)-receptor stimulation was suppressed by inhibitors of cAMP dependent protein kinase (PKA), implying GABA(C) receptor mediated PKA activation ... To test the possibility of GABA(C) receptor mediated PKA activation, association of GABA(C) receptor with A-kinase anchoring proteins ( AKAPs ) and effect of an AKAP antisense oligonucleotide on the PKA activation were examined in primary cultured rat hippocampal neurons ... To test the possibility of GABA(C) receptor mediated PKA activation, association of GABA(C) receptor with A-kinase anchoring proteins ( AKAPs ) and effect of an AKAP antisense oligonucleotide on the PKA activation were examined in primary cultured rat hippocampal neurons ... Furthermore, antisense oligonucleotide of AKAP220 suppressed such GABA ( C ) stimulation induced PKA activation, suggesting that GABA(C)-receptor stimulation activates PKA via AKAP220
Nicolas et al., Cardiovasc Res 2008 : We propose that the KCNQ1-KCNE1 channel directly interacts with microtubules and that this interaction plays a major role in coupling PKA dependent phosphorylation of KCNQ1 with I ( Ks ) activation
Gauthier et al., J Biol Chem 2008 : Likewise, small hairpin RNA mediated silencing of adipose tissue triglyceride lipase inhibited both forskolin stimulated lipolysis and AMPK activation but not that of PKA
Barlow et al., Apoptosis 2008 : Application of H2O2 increased nuclear accumulation of PKA, and inhibition of PKA with H89 reduced oxidant mediated phosphorylation of both CREB and ERK1/2 ... Application of H2O2 increased nuclear accumulation of PKA, and inhibition of PKA with H89 reduced oxidant mediated phosphorylation of both CREB and ERK1/2
Torii et al., Cell Signal 2008 : On the other hand, loss of function of protein kinase A (PKA), induced by treatment with PKA inhibitors, siRNA mediated knocking down of endogenous PKA catalytic subunits, or enforced expression of dominant negative PKA inhibited the Wnt5a anti-apoptotic activity, indicating the involvement of PKA in the Wnt5a anti-apoptotic activity ... Based on another finding indicating that Wnt5a upregulated PKA mediated phosphorylation of glycogen synthase kinase-3beta ( GSK-3beta ) at serine 9 that caused inactivation of GSK-3beta and subsequently resulted in activation of the beta-catenin pathway, we have speculated that the Wnt5a anti-apoptotic activity may be partially mediated by PKA mediated phosphorylation of GSK-3beta and subsequent activation of the beta-catenin pathway
Yang et al., Arch Pharm Res 2008 (Breast Neoplasms) : We previously reported that cAMP dependent protein kinase (PKA) mediated activator protein-2 (AP-2) activation was responsible for the expression of Her-2/ErbB2 in p53 inactivated mammary epithelial cells ( Yang et al., 2006 ) ... In the present study, we tested the hypothesis that PKA plays a role in the expression of ErbB2 in tamoxifen-resistant breast cancer cells ... In contrast, PKA inhibition by H-89 or cAMP dependent protein kinase inhibitor l gamma overexpression increased the expression levels of ErbB2 in TAM-resistant MCF-7 ( TAMR-MCF-7 ) cells
Nadella et al., Cancer Res 2008 (Multiple Endocrine Neoplasia...) : Finally, this down-regulation of vimentin was recapitulated in the adrenal nodules of CNC patients, confirming an unexpected and previously unrecognized role for PKA in MET
Huang et al., Am J Respir Cell Mol Biol 2008 : On the other hand, PKA inhibition of collagen was dependent on inhibition of protein kinase C-delta
Gerits et al., Cell Signal 2008 : The first- and best characterized interconnections are the PKA dependent inhibition of the MAPKs ERK1/2 mediated by RAF-1, and PKA induced activation of ERK1/2 interceded through B-RAF
Kim et al., J Cell Biochem 2008 : The siRNA mediated inhibition of PKA and ERK1 resulted in significant reduction of EX-4 induced Egr-1 expression
Mandal et al., Am J Physiol Cell Physiol 2008 (Ion Channel Gating) : Taken together, the results are consistent with increased capacitative calcium entry and subsequent cAMP-PKA dependent stimulation of NHE1 in ouabain treated cells
Tawfeek et al., Endocrinology 2008 : In the current study, we explored the role of PTH/PTHrP receptor phosphorylation and PKA in PTH activation of PLC ... In the current study, we explored the role of PTH/PTHrP receptor phosphorylation and PKA in PTH activation of PLC ... Interestingly, PTH and forskolin ( adenylate cyclase/PKA pathway activator ) treatment causes an increase in PLCbeta3 phosphorylation at the Ser1105 inhibitory site and that increase is blocked by the PKA inhibitor, H89
Barinova et al., Klin Lab Diagn 2008 (Diabetes Mellitus, Type 2...) : Patients with the diabetic foot were shown to have impaired adrenergic responsiveness, accompanied by reduced eNOS activity and PKA inhibition
Hsiung et al., J Neurosci Res 2008 : Our data suggest a central role of cAMP/PKA dependent PP2A in shifting the homeostasis of intracellular signaling downstream of activated 5-HT(1A) receptor toward cell death in biological systems linked to neuropsychiatric disorders
Schnizler et al., J Neurosci 2008 (Hyperalgesia) : In functional studies, PKA stimulation with forskolin markedly reduced desensitization of TRPV1
Yang et al., Am J Physiol Gastrointest Liver Physiol 2008 : TGF-beta also induced activation of the PKA dependent transcription factor CREB
Atsuta et al., Int J Cardiol 2009 : Experiments using various pharmacological inhibitors for protein kinases showed that activation of cAMP dependent protein kinase (PKA) was involved in BPS mediated regulation of VEGF and PAI-1 mRNA expression
Hichami et al., Mediators Inflamm 1996 : We investigated the effects of specific inhibitors of cAMP dependent protein kinase (PKA) and cGMP dependent protein kinase ( PKG ) on the inhibitory activity of phosphodiesterase ( PDE ) type 4 inhibitors and of the cell permeable analogue of cAMP, db-cAMP on LPS induced TNF-alpha release from human mononuclear cells
Schmidt et al., BMC physiology 2008 : By using cAMP analogs that activated either protein kinase A (PKA) or Epac and the application of PKA-inhibitors, we found that cAMP induced augmentation of InsP3 induced Ca2+ release was mediated by PKA not by Epac
Lee et al., Neurochem Res 2008 : To examine PKA involvement in the effects of db-cAMP on MMP-9 and tPA activity, we added the PKA inhibitors, H89 or rp-cAMP, along with db-cAMP, and they inhibited db-cAMP mediated changes in tPA activity without affecting MMP-9 activity
Bruss et al., J Biol Chem 2008 : This effect of PDE4D ablation was in large part due to inactivation of a negative feedback mechanism consisting of the PKA mediated activation of PDE4D in response to elevated cAMP levels, as indicated by experiments using the cAMP dependent protein kinase inhibitors H89 and PKI
Paveto et al., Cell Biol Int Rep 1991 (Second Messenger Systems) : The effect of these hormones in permeabilized cells of the fungus produced a 2-3 fold increase in the Mg2+ , GTP dependent adenylyl cyclase activity as well as full activation of the cAMP dependent protein kinase (PKA) activity
Escamilla-Hernandez et al., Mol Endocrinol 2008 : mRNA levels for P450scc and 3beta-HSD were induced to a similar extent by FSH and PKA-CQR , whereas mRNA levels for P450arom and the LHr were induced to a greater extent by FSH
Hong et al., J Biol Chem 2008 : Here we show that ( i ) upstream regulators, PIK and PDK1, are not the target ( s ) of the cAMP inhibitory action ; ( ii ) constitutively active Akt and calyculin A-stimulated Akt are resistant to cAMP inhibition, suggesting the action of a phosphatase ; ( iii ) cAMP increases the rate of Akt dephosphorylation, directly implicating an Akt-phosphatase ; ( iv ) Epac- and protein kinase A (PKA)-specific analogs synergistically inhibit Akt, indicating the involvement of both cAMP dependent effector pathways ; ( v ) H89 and dominant negative Epac 279E block cAMP-inhibitory action ; ( vi ) Epac associates in a complex with Akt and PP2A, and the associated-phosphatase activity is positively modulated by cAMP in a PKA- and Rap1 dependent manner ; ( vii ) like its action on Akt inhibition, PKA- and Epac-specific analogs synergistically activate Epac associated PP2A ; and ( viii ) dominant negative PP2A blocks cAMP-inhibitory action ... Here we show that ( i ) upstream regulators, PIK and PDK1, are not the target ( s ) of the cAMP inhibitory action ; ( ii ) constitutively active Akt and calyculin A-stimulated Akt are resistant to cAMP inhibition, suggesting the action of a phosphatase ; ( iii ) cAMP increases the rate of Akt dephosphorylation, directly implicating an Akt-phosphatase ; ( iv ) Epac- and protein kinase A (PKA)-specific analogs synergistically inhibit Akt, indicating the involvement of both cAMP dependent effector pathways ; ( v ) H89 and dominant negative Epac 279E block cAMP-inhibitory action ; ( vi ) Epac associates in a complex with Akt and PP2A, and the associated-phosphatase activity is positively modulated by cAMP in a PKA- and Rap1 dependent manner ; ( vii ) like its action on Akt inhibition, PKA- and Epac-specific analogs synergistically activate Epac associated PP2A ; and ( viii ) dominant negative PP2A blocks cAMP-inhibitory action
Vorland et al., Platelets 2008 : Further studies using inhibitors as well as specific activators of protein kinases A and G suggest a role for PKA in the inhibition of thrombin activated PLD ... We found that PGE ( 1 ), forskolin and the PKA activator inhibited PLD1 translocation in thrombin stimulated platelets, and that the PKG-activator had no effect ... The present results suggest a role for PKA in the regulation of thrombin induced PLD1 activity and translocation in platelets
Caetano et al., J Neurosci 2008 (MAP Kinase Signaling System) : The secreted cochaperone STI1 triggers activation of protein kinase A (PKA) and ERK1/2 signaling by interacting with the cellular prion ( PrP ( C ) ) at the cell surface, resulting in neuroprotection and increased neuritogenesis
Goldfinger et al., Circ Res 2008 (Mechanotransduction, Cellular) : Moreover, shear induced localized activation of the small GTPase Rac1 , which specifies the directionality of endothelial alignment, was similarly blocked by PKA inhibitors
Kaur et al., Am J Physiol Lung Cell Mol Physiol 2008 : In each case, repression of these inflammatory indexes was prevented by adenoviral overexpression of PKIalpha , a highly selective PKA inhibitor
Tang et al., Cell Signal 2008 : ERK ( 1/2 ) activation involves Gq/phospholipase C (PLC)/protein kinase C ( PKC ), Gs/adenylyl cyclase ( AC ) /cAMP/protein kinase A (PKA) and Gi cascades ; however, the Gq/PLC/PKC pathway, as well as PKA is not required for OX2R mediated p38 MAPK activation ... ERK ( 1/2 ) activation involves Gq/phospholipase C (PLC)/protein kinase C ( PKC ), Gs/adenylyl cyclase ( AC ) /cAMP/protein kinase A (PKA) and Gi cascades ; however, the Gq/PLC/PKC pathway, as well as PKA is not required for OX2R mediated p38 MAPK activation ... ERK ( 1/2 ) activation involves Gq/phospholipase C (PLC)/protein kinase C ( PKC ), Gs/adenylyl cyclase ( AC ) /cAMP/protein kinase A (PKA) and Gi cascades ; however, the Gq/PLC/PKC pathway, as well as PKA is not required for OX2R mediated p38 MAPK activation
Vasquez et al., J Cell Physiol 2008 : DbcAMP stimulated SGK1 mRNA expression was decreased by actinomycin D and mRNA and protein expressions were attenuated by PKA inhibitors ( H-89 and KT5720 )
Hook et al., Neuropeptides 2008 : The goal of this study was to understand how different neuropeptides may be similarly or differentially regulated by protein kinase A (PKA) and protein kinase C ( PKC ) intracellular signaling mechanisms
Islam et al., J Biol Chem 2008 : cAMP dependent protein kinase A (PKA) signaling induces TNFR1 exosome-like vesicle release via anchoring of PKA regulatory subunit RIIbeta to BIG2 ... We show that 8-bromo-cyclic AMP induced the release of full-length, 55-kDa TNFR1 within exosome-like vesicles via a protein kinase A (PKA) dependent mechanism
Lu et al., Mol Cell Biol 2008 (HIV Infections) : All-trans retinoic acid ( atRA ) reverses the HIV induced podocyte phenotype by activating cyclic AMP ( cAMP ) /protein kinase A (PKA) and inhibiting MAPK1,2
Chappe et al., J Pharmacol Exp Ther 2008 : VIP induced accumulation of apical CFTR was mimicked by phorbol ester but not by forskolin, and it was blocked by the protein kinase ( PK ) C inhibitors bisindolylmaleimide X ( BisX ) or chelerythrine chloride but not by the PKA inhibitor N- [ 2- ( p-bromocinnamylamino ) ethyl ] -5-isoquinolinesulfonamide dihydrochloride ( H89 )
Mayama et al., Experimental and clinical cardiology 2007 : At the beginning of fibrillation, an increase in the cardiac tissue AII level, an augmentation of the protein kinase C (PKC)-epsilon activity, the presence of PKC mediated hyperphosphorylation, a suppression of the PKA mediated phosphorylation of Cx43 and a reduction in the expression of Cx43 at the gap junction were observed
Matsumura et al., Experimental and clinical cardiology 2006 : The effects of hypoxia, intracellular Ca ( 2+ ) overload and intracellular acidosis on the PKA mediated phosphorylation of connexin 43 (Cx43) were examined in relation to the function of the cardiac gap junction ... Cyclic AMP or the activation of PKA promotes the intercellular electrical coupling that accompanies an augmentation of the PKA mediated phosphorylation of Cx43 ... Electrical cell-to-cell decoupling and reduction of the PKA mediated phosphorylation of Cx43 were dependent on the progression of hypoxia ... In cardiac ventricular muscle cells, cyclic AMP or the activation of PKA promotes electrical cell-to-cell coupling through the gap junction due to an augmentation of the PKA mediated phosphorylation of Cx43 in the early stage of hypoxia, as well as in normoxia ... The suppression of PKA mediated phosphorylation of Cx43 during hypoxia may be caused by an increase in the intracellular ionic strength of Ca ( 2+ ) and H ( + )
Li et al., J Membr Biol 2008 : Here, we report that the PKA-RII-specific AKAP inhibitory peptide AKAP-IS perturbs the distribution of PKA-RII and diminishes the PKA dependent phosphorylation of HERG protein
Gomes et al., Peptides 2008 : We investigated the hypothesis that PI-PLCbeta could be the target for Ado induced PKA activation ... These data demonstrate that Ado induced PKA activation reduces Ang II-induced stimulation of PI-PLCbeta
Sayasith et al., Endocrinology 2008 : Collectively, this study is the first to describe that the ovulatory process in cows is associated with a gonadotropin dependent induction of TNFAIP6 in ovarian follicles and provide the molecular basis through which AP1 and CRE sites and PKA activation played important roles in the regulation of TNFAIP6 in granulosa cells
Grueb et al., Ophthalmic Res 2008 : In corneal epithelial and endothelial cells, beta-adrenergic stimulation leads to activation of PKA via stimulation of adenylyl cyclase, and alpha(2A)-adrenoceptor stimulation inhibits PKA activity via inhibition of adenylyl cyclase
Yokoyama et al., J Biol Chem 2008 : A specific EP4 agonist, ONO-AE1-329, increased Rap1 activity in the presence of a PKA inhibitor, PKI- ( 14-22 ) -amide, in DA smooth muscle cells
Evaul et al., J Biol Chem 2008 (MAP Kinase Signaling System) : LH-induced increases in cAMP and cAMP dependent protein kinase (PKA) activity mediated trans-activation of the EGF receptor and subsequent mitogen activated protein kinase ( MAPK ) activation, ultimately leading to StAR phosphorylation and mitochondrial translocation ... Steroidogenesis in Leydig cells was unaffected by MMP inhibitors, suggesting that cAMP and PKA trans activated EGF receptors in an intracellular fashion
Izumi et al., American journal of physiology. Renal physiology 2008 (MAP Kinase Signaling System) : The increase of V2R promoter activity by hypertonicity was significantly inhibited by a JNK inhibitor ( SP600125 ) and PKA inhibitor ( H89 )
Hajishengallis et al., Proc Natl Acad Sci U S A 2008 (Bacteroidaceae Infections) : This is accomplished through its surface fimbriae, which induce CXCR4/TLR2 co-association in lipid rafts and interact with both receptors : Binding to CXCR4 induces cAMP dependent protein kinase A (PKA) signaling, which in turn inhibits TLR2 mediated proinflammatory and antimicrobial responses to the pathogen
Luke et al., Purinergic Signal 2008 : Inhibitors of protein kinase C ( PKC ), protein kinase A (PKA) , Ca ( 2+ ) /calmodulin dependent protein kinase II ( CaMKII ), and phosphoinositide-3 kinase (PI3K) had no effect on ATP mediated ERK1/2 phosphorylation
Monje et al., J Biol Chem 2008 : Here, we report that the activation of protein kinase A (PKA) , but not that of exchange protein activated by cAMP ( EPAC ), enhances S-phase entry of SCs by synergistically enhancing the ligand dependent tyrosine phosphorylation/activation of the neuregulin co-receptor, ErbB2-ErbB3 ... Here, we report that the activation of protein kinase A (PKA) , but not that of exchange protein activated by cAMP ( EPAC ), enhances S-phase entry of SCs by synergistically enhancing the ligand dependent tyrosine phosphorylation/activation of the neuregulin co-receptor, ErbB2-ErbB3 ... The role of PKA in neuregulin-ErbB signaling was confirmed using PKA inhibitors, pathway-selective cAMP analogs, and natural ligands stimulating PKA activity in SCs, such as adenosine and epinephrine ... The role of PKA in neuregulin-ErbB signaling was confirmed using PKA inhibitors, pathway-selective cAMP analogs, and natural ligands stimulating PKA activity in SCs, such as adenosine and epinephrine ... Two basic observations defined the synergistic action of PKA as `` gating '' for neuregulin-ErbB signaling : 1 ) the activation of PKA was not sufficient to induce S-phase entry or the activation of either ErbB2 or ErbB3 ; and 2 ) the presence of neuregulin was strictly required to ignite ErbB activation and thereby ERK and Akt signaling ... Two basic observations defined the synergistic action of PKA as `` gating '' for neuregulin-ErbB signaling : 1 ) the activation of PKA was not sufficient to induce S-phase entry or the activation of either ErbB2 or ErbB3 ; and 2 ) the presence of neuregulin was strictly required to ignite ErbB activation and thereby ERK and Akt signaling ... Two basic observations defined the synergistic action of PKA as `` gating '' for neuregulin-ErbB signaling : 1 ) the activation of PKA was not sufficient to induce S-phase entry or the activation of either ErbB2 or ErbB3 ; and 2 ) the presence of neuregulin was strictly required to ignite ErbB activation and thereby ERK and Akt signaling ... However, PKA directly phosphorylated ErbB2 on Thr-686, a highly conserved intracellular regulatory site that was required for the PKA mediated synergistic enhancement of neuregulin induced ErbB2-ErbB3 activation and proliferation in SCs ... However, PKA directly phosphorylated ErbB2 on Thr-686, a highly conserved intracellular regulatory site that was required for the PKA mediated synergistic enhancement of neuregulin induced ErbB2-ErbB3 activation and proliferation in SCs
Kostic et al., Stress 2008 : Thus, PKA mediated phosphorylation of StAR protein is an important mechanism in the adaptive response of Leydig cells to repeated IMO
Honjo et al., Clin Neuropathol 2008 (Multiple System Atrophy) : DARPP32 has been identified as a target for dopamine and PKA in the striatum
Shirakawa et al., Nature structural & molecular biology 2008 : These data imply that PKA mediated phosphorylation of A3G can regulate the interaction between A3G and Vif
Woo et al., Mol Pharmacol 2009 : The inefficient G ( i ) signaling with the R, R isomers is not caused by the inability of the R, R isomers to trigger the protein kinase A (PKA) mediated phosphorylation of the beta(2)-adrenoceptor , because the R, R isomers also markedly increased phosphorylation of the receptor at serine 262 by PKA
Lee et al., Biochemistry 2008 (MAP Kinase Signaling System) : Inhibiting PKA modestly attenuated ERK1/2 activation, while inhibition of Src family tyrosine kinases with PP2 abolished the response
Nishi et al., J Neurosci 2008 : Distinct roles of PDE4 and PDE10A in the regulation of cAMP/PKA signaling in the striatum ... Inhibition of PDE10A by papaverine activated cAMP/PKA signaling in both striatonigral and striatopallidal neurons, resulting in potentiation of dopamine D(1) receptor signaling and inhibition of dopamine D(2) receptor signaling
Basu et al., Mol Cell 2008 : Comparison of bony fish versus amphibian and mammalian AIDs suggests evolutionary divergence from constitutive to PKA regulated RPA/AID interaction
Cardone et al., PloS one 2008 (Cell Transformation, Viral) : HPV16 E7-dependent transformation activates NHE1 through a PKA-RhoA induced inhibition of p38alpha ... Further, using pharmacological agents and transient transfections with dominant interfering, constitutively active, phosphorylation negative mutants and siRNA strategy to modify specific upstream signal transduction components that link HPV16 E7 oncogenic signals to up-regulation of the NHE1, we demonstrate that the stimulation of NHE1 activity is driven by an early rise in cellular cAMP resulting in the down-stream inhibition of p38 MAPK via the PKA dependent phosphorylation of the small G-protein, RhoA , and its subsequent inhibition
Ren et al., Genomics 2009 : Mutagenesis and cotransfection experiments showed that PKA regulation of this c-fos intronic element was mediated by two adjacent CRE-like sequences and the transcription factor CREB ... Mutagenesis and cotransfection experiments showed that PKA regulation of this c-fos intronic element was mediated by two adjacent CRE-like sequences and the transcription factor CREB
Hattori et al., Evidence-based complementary and alternative medicine : eCAM 2010 : Thus, AMP N ( 1 ) -oxide elicited neuronal differentiation of PC12 cells, as evidenced by generation of neurites, and inhibited cell growth through adenosine A(2A) receptor mediated PKA signaling, which may be responsible for characteristic actions of RJ
Zhang et al., Biochem Biophys Res Commun 2008 : This study investigated the regulation of Kir7.1 by PKA and PKC
Rosenkranz et al., J Physiol 2009 : Both forms of plasticity are blocked by inhibition of PKA and occluded by interfering with cAMP degradation, consistent with a PKA mediated increase in MAPK activity following induction of LTP
McAlees et al., Mol Cell Biol 2009 : beta ( 2 ) AR agonists, cAMP elevating agents, and PKA inhibitors were used to show that beta ( 2 ) AR stimulation resulted in a PKA dependent increase in p38 MAPK phosphorylation ... beta ( 2 ) AR agonists, cAMP elevating agents, and PKA inhibitors were used to show that beta ( 2 ) AR stimulation resulted in a PKA dependent increase in p38 MAPK phosphorylation ... Thus, beta ( 2 ) AR stimulation on a B cell phosphorylates and inactivates HePTP in a Gs/cAMP/PKA dependent manner to release bound p38 MAPK, making more available for phosphorylation and subsequent IgE regulation
Mao et al., Brain Res Bull 2009 (MAP Kinase Signaling System) : However, the increase in ERK1/2 phosphorylation was partially blocked by a protein kinase A (PKA) inhibitor
Misior et al., Am J Respir Cell Mol Biol 2009 : Recent studies have suggested that GCs render agents of inflammation such as IL-1 beta and TNF-alpha mitogenic to ASM, via suppression of ( antimitogenic ) induced cyclooxygenase-2 dependent PKA activity
Banales et al., Hepatology 2009 (Cysts...) : On the other hand, both PKA and Epac activation of cystic structures generated by normal and PCK cholangiocytes when cultured under three-dimensional conditions resulted in increased cyst growth, particularly in PCK-cholangiocyte derived cysts
Faruque et al., Am J Physiol Cell Physiol 2009 : Immunoflurescent analysis also indicated that TAT-AKAPis significantly affected PKA subcellular localization
Morgan et al., Proc Natl Acad Sci U S A 2008 : Our results demonstrate that PKA in CalphaM120A mutant sperm is rapidly and reversibly inhibited by 1NM-PP1 and that this blockade has selective and time dependent effects on multiple aspects of capacitation
Kurokawa et al., Channels (Austin) 2009 : beta-adrenergic receptor mediated IKs upregulation, a functional consequence of PKA phosphorylation of the KCNQ1 amino terminus ( N-T ), requires co-expression of KCNQ1/Yotiao with KCNE1
Tian et al., Proc Natl Acad Sci U S A 2008 : Thus, palmitoylation gates BK channel regulation by PKA phosphorylation
Ginty et al., J Biol Chem 1991 : Here we demonstrate that both cAMP- and calcium mediated induction of c-fos and egr1 are dependent on PKA activity
Dyson et al., Mol Cell Endocrinol 2009 (Leydig Cell Tumor) : This includes the PKA dependent expression and activation of the steroidogenic acute regulatory protein ( STAR ), which mediates the rate limiting step of steroidogenesis by inducing the transfer of cholesterol from the outer to the inner mitochondrial membrane
Barclay et al., Int J Cancer 2009 (Breast Neoplasms) : In addition, prolactin induced SOCS3 promoter activation requires PKA stimulated Sp1 binding to the GC-rich region of the promoter
Subramanian et al., J Cell Biochem 2009 : These results indicate that PKA mediated phosphorylation of SGLT1 changes the conformation of the empty carrier and the glucose carrier complex, probably causing the increase in transport affinity
Park et al., Carcinogenesis 2009 (Cell Transformation, Neoplastic...) : The upregulation of MMP-2 induced by phosphatidic acid ( PA ), the product of PLD, was mediated by protein kinase C ( PKC ), protein kinase A (PKA) , nuclear factor-kappaB (NF-kappaB) and Sp1 and it enhanced glioma cell invasion
Manna et al., Mol Immunol 2009 (Neoplasms) : It inhibited phosphorylation of Rel A ( p65 ) by inhibiting activity of protein kinase A (PKA) ... The PKA inhibition was independent of adenylate cyclase activity or cAMP level ... P(3)-25 inhibited almost 80 % PKA activity at 100 nM concentration, having an IC ( 50 ) at 10.5 nM ... Our results suggest that P(3)-25 inhibits PKA activity followed by decreased phosphorylation of p65 and transcriptional activity of NF-kappaB thereby decreasing antiapoptotic proteins resulting in induction of apoptosis in NF-kappaB expressing and doxorubicin-resistant cells
Takei et al., J Mol Endocrinol 2009 : On the other hand, PTH and phorbol 12,13-myristic acetate, a specific PKC activator, but not 8-bromo-cyclic AMP, a specific PKA activator, reduced the mRNA levels of Stc2
Guan et al., Regul Pept 2009 : These results suggest that the VIP mediated wound repair of HBECs is associated with activation of CREB via PKA and ERK dependent pathway
Park et al., Biochem Biophys Res Commun 2009 : HO-1 expression by PGE ( 2 ) was inhibited by LY294002, PI3K inhibitor and H89, PKA inhibitor ... HO-1 expression by PGE ( 2 ) was inhibited by LY294002, PI3K inhibitor and H89, PKA inhibitor ... Taken together, we conclude that PGE ( 2 ) induces HO-1 protein expression through PKA and PI3K signaling pathways via EP ( 2 ) receptor in C6 cells
Beck et al., Biochem Pharmacol 2009 (Inflammation) : Although H89 can inhibit both MSK1 and PKA, TNF does not activate PKA ( EC 2.7.11.11 ) and as such PKA inhibition does not mediate H89 instigated repression of TNF stimulated gene expression
Beyenbach et al., J Exp Biol 2009 : Supporting the putative role of PKA in the AK-III induced activation of the V-type H ( + ) ATPase is the effect of H89, an inhibitor of PKA, on fluid secretion
Bertran-Gonzalez et al., Biol Psychiatry 2009 : Thus, blockade of D2Rs promotes histone H3 phosphorylation through the A2AR mediated activation of Galpha ( olf ) and inhibition of protein phosphatase-1 (PP-1) through the PKA dependent phosphorylation of DARPP-32
Lee et al., Toxicology 2009 : Taken together, these results suggest that PKA dependent NF-kappaB inhibition stimulates 15d-PGJ ( 2 ) -mediated mitochondrial apoptotic pathway through alterations in expression of the NF-kappaB target genes Bax
Omar et al., Cell Signal 2009 : Taken together, these data suggest that PDE3B and PDE4 regulate cAMP pools that affect the activation/phosphorylation state of AMPK and that the effects of cyclic AMP on AMPK involve Epac1, PKA and lipolysis
Nierth-Simpson et al., Endocrinology 2009 (Leiomyoma...) : cAMP-PKA signaling is rapidly activated only in UtSM cells with E2 and inhibits ERK1/2 activation and proliferation
Dai et al., Thromb Res 2009 : PKA inhibition mediated GPIbalpha shedding was reversed by PKA activator forskolin and partially inhibited by membrane-permeable calpain inhibitors
Khoutorsky et al., Learn Mem 2009 (Calcium Signaling) : Inhibition of CaM kinases, PKA , and MAPK also reduced PTP at this synapse
Kadoshima-Yamaoka et al., Immunol Lett 2009 : These findings suggest that PDE7A may regulate function of activated T cells in a cAMP/PKA dependent manner, and that PDE4 might share the role
Hallows et al., Am J Physiol Cell Physiol 2009 : AMP activated protein kinase inhibits alkaline pH- and PKA induced apical vacuolar H+-ATPase accumulation in epididymal clear cells ... We have shown previously that protein kinase A (PKA) , acting downstream of soluble adenylyl cyclase stimulation by alkaline luminal pH or HCO ( 3 ) ( - ), induces V-ATPase apical membrane accumulation in clear cells ... Here we examined whether the metabolic sensor AMP activated protein kinase (AMPK) regulates this PKA induced V-ATPase apical membrane accumulation
Kadota et al., Microbiol Immunol 1991 : In contrast to its effects on PKC, CKS-17-HSA did not inhibit the activity of adenosine 3 ' : 5'-cyclic monophosphate ( cyclic AMP ) -dependent protein kinase (PK-A) nor the calcium and phospholipid independent form of PKC ( PK-M )
Hanft et al., Am J Physiol Heart Circ Physiol 2009 : PKA increased the phosphorylation of myosin binding protein C and cardiac troponin I , as assessed by autoradiography ... PKA increased the phosphorylation of myosin binding protein C and cardiac troponin I, as assessed by autoradiography
Maeda et al., J Biochem 2009 : Finally, PKA inhibited leptin gene transcription without new protein synthesis
Ahn et al., J Pharmacol Exp Ther 2009 : Interactions of protein kinases activated by intracellular Ca ( 2+ ) release downstream to PLC modulate the phosphorylation state of GluR1 on Ser831 and Ser845 : phosphorylation of GluR1 on Ser831 is up-regulated by the protein kinase C and calcium-calmodulin dependent protein kinase ( CaMK ) /c-Jun N-terminal kinase (JNK) pathways, whereas phosphorylation of GluR1 on Ser845 is up-regulated by the protein kinase A (PKA), PKA/ERK1/2 , and PKA/JNK pathways
Zheng et al., J Neurophysiol 2009 : PKA and the Ca ( 2+ ) -calmodulin dependent protein kinases ( CaMKs ) II and IV are activated early in conditioning and are required for acquisition and expression of conditioned responses (CRs)
Liu et al., Mol Endocrinol 2009 : cAMP/PKA stimulation of ERRalpha activation of the SP-A promoter was blocked by the PKA inhibitor, H89, whereas the MAPK P38 inhibitor, SB203580, and the MAPK kinase inhibitor, PD98059, had negligible to modest effects ... Collectively, these findings indicate that cAMP/PKA signaling enhances ERRalpha phosphorylation and nuclear localization, recruitment to the SP-A promoter, and interaction with PKAcat and SRC-2, resulting in the up-regulation of SP-A gene transcription ... Collectively, these findings indicate that cAMP/PKA signaling enhances ERRalpha phosphorylation and nuclear localization, recruitment to the SP-A promoter, and interaction with PKAcat and SRC-2 , resulting in the up-regulation of SP-A gene transcription
Shigemura et al., Prostate 2009 (MAP Kinase Signaling System...) : We observed ERK kinase inhibitor, U0126, but not phosphatidylinositol 3-kinase (PI-3K), LY294002, or protein kinase A (PKA) inhibitor, H-89, inhibited stromal CM or androgen induced PSA promoter luciferase activities, and anchorage independent growth of LNCaP cells
Brubaker et al., Adv Exp Med Biol 1991 : Pancreatic glucagon secretion in response to PKA is subject to further modulation by prevailing glucose concentrations
Cao et al., J Neurosci 2009 (Pain) : Upstream, NMDA receptor, adenylyl cyclase ( AC ) and phosphokinase A (PKA) activators activated ERK in rACC slices ... Downstream, phosphorylation of cAMP response element binding protein ( CREB ) was induced in the rACC by formalin injection and by NMDA, AC and PKA activators in brain slices, which was suppressed by MEK inhibitors
French et al., International journal of medical sciences 2009 (Melanoma...) : PKC and PKA phosphorylation affect the subcellular localization of claudin-1 in melanoma cells
Wang et al., Biochim Biophys Acta 2009 : AM/AMBP-1 prevented a reduction of cAMP levels and protein kinase A (PKA) activity in neural cells after hypoxia exposure
Hsu et al., FEBS Lett 2009 (Calcinosis) : A six-hour T0901317 treatment augmented the PKA induced expression of the phosphate transporter Pit-1 , a positive regulator of mineralization, suggesting a direct role
Shapiro et al., Curr Biol 2009 (Candidiasis) : Hsp90 regulates morphogenetic circuitry by repressing Ras1-PKA signaling
Qian et al., Glia 2009 : Furthermore, we found ERK inhibition, but not protein kinase A (PKA) inhibition , significantly abolished salmeterol induced superoxide production, p47(phox) translocation, and its ability to mediate neurotoxicity
Iliopoulos et al., Cancer Res 2009 (Adrenal Cortex Diseases...) : Using a PPNAD cell line, we showed that miR-449 was up-regulated and identified its direct target, WNT1-inducible signaling pathway protein 2 ( WISP2 ) ; in addition, pharmacologic inhibition of PKA resulted in the up-regulation of miR-449 leading to the suppression of WISP2
Ong et al., Br J Pharmacol 2009 : A role for intracellular cAMP/PKA in enhancing GLP-1 release in response to overexpression of D556A-PDE4D5 mutant was demonstrated by the finding that the PKA inhibitor H89 reduced both basal and glucose induced GLP-1 release by 37 % and 39 %, respectively, from C1 GLUTag cells
Dhillon et al., Regul Pept 2009 : Pharmacological inhibitors of the MAPK and PKA signal transduction pathways attenuated the NPY mediated increase in GnRH transcription
Lai et al., Cell Biochem Funct 2009 : Our results suggest that rapid PTH inhibition of ERK1/2 activity is mediated by PKA dependent inhibition of c-Raf activity and that stimulation of MKP-1 may contribute to maintaining pERK1/2 inhibition over prolonged time
Namkoong et al., Cell Signal 2009 : Forskolin increases angiogenesis through the coordinated cross-talk of PKA dependent VEGF expression and Epac mediated PI3K/Akt/eNOS signaling ... Forskolin increases angiogenesis through the coordinated cross-talk of PKA dependent VEGF expression and Epac mediated PI3K/Akt/eNOS signaling ... Forskolin increases angiogenesis through the coordinated cross-talk of PKA dependent VEGF expression and Epac mediated PI3K/Akt/eNOS signaling ... Forskolin increases angiogenesis through the coordinated cross-talk of PKA dependent VEGF expression and Epac mediated PI3K/Akt/eNOS signaling ... These results suggest that forskolin stimulates angiogenesis through coordinated cross-talk between two distinct pathways, PKA dependent VEGF expression and Epac dependent ERKactivation and PI3K/Akt/eNOS/NO signaling ... These results suggest that forskolin stimulates angiogenesis through coordinated cross-talk between two distinct pathways, PKA dependent VEGF expression and Epac dependent ERKactivation and PI3K/Akt/eNOS/NO signaling ... These results suggest that forskolin stimulates angiogenesis through coordinated cross-talk between two distinct pathways, PKA dependent VEGF expression and Epac dependent ERKactivation and PI3K/Akt/eNOS/NO signaling ... These results suggest that forskolin stimulates angiogenesis through coordinated cross-talk between two distinct pathways, PKA dependent VEGF expression and Epac dependent ERKactivation and PI3K/Akt/eNOS/NO signaling
Al-Wadei et al., Anticancer Drugs 2009 (Carcinoma, Pancreatic Ductal...) : Our data suggest that propranolol may prevent the development of PDAC by blocking cAMP dependent intracellular signaling, cAMP dependent release of epidermal growth factor, and PKA dependent release of vascular endothelial growth factor while additionally downregulating the alpha7 nAChR by inhibiting cAMP mediated subunit assembly
Tumati et al., Neuroreport 2009 (Hyperalgesia...) : Sustained cannabinoid agonist treatment augments CGRP release in a PKA dependent manner
Perry et al., Am J Physiol Gastrointest Liver Physiol 2009 (Ion Channel Gating) : Since many channel proteins are regulated by phosphorylation, we evaluated the roles of protein kinase A (PKA) and phosphatases in the modulation of apical BK channel activity in surface colonocytes from rat distal colon using patch-clamp techniques, having first increased channel abundance by chronic dietary K+ enrichment
Paz et al., Methods Enzymol 2009 : The activation of PKA triggers the posttranslational modification of the mitochondrial acyl-CoA thioesterase ( Acot2 ), which releases arachidonic acid ( AA ) in the mitochondria, and the activation of a kinase cascade that leads to the phoshorylation of the steroidogenic acute regulatory (StAR) protein
Rodrigues et al., Mol Cell Endocrinol 2009 (MAP Kinase Signaling System) : Also, ERK1/2 activation is not significantly affected by protein kinase A (PKA) , protein kinase C ( PKC ) and protein kinase B or Akt ( Akt/PKB ) specific inhibitors ... Also, ERK1/2 activation is not significantly affected by protein kinase A (PKA) , protein kinase C ( PKC ) and protein kinase B or Akt ( Akt/PKB ) specific inhibitors
Matsuba et al., J Gen Physiol 2009 : Reconstitution of cardiac muscle with the fast skeletal Tn complex ( sTn ) not only increased Ca2+ sensitivity, but also abolished the Ca2+ desensitizing effect of PKA, supporting the view that the phosphorylation of cTnI, but not that of other myofibrillar proteins, such as myosin binding protein C, primarily underlies the PKA induced Ca2+ desensitization in cardiac muscle
Boudreau et al., J Neurochem 2009 (Cocaine-Related Disorders...) : We focused on calcium/calmodulin dependent protein kinase II ( CaMKII ), extracellular signal regulated protein kinase ( ERK ), and protein kinase A (PKA)
Bellis et al., Arterioscler Thromb Vasc Biol 2009 (Disease Models, Animal...) : In addition, in early and late PC, PKA physically interacted with the phosphorylated form of Akt, suggesting that PKA is required for Akt phosphorylation ... Late ischemic PC protects BAECs against hypoxia through PKA- and PI3K dependent activation of Akt
Wojtal et al., J Biol Chem 2009 : However, oncostatin M signaling does not stimulate PKA holoenzyme dissociation in living cells
Goto et al., Cell Immunol 2009 : These results suggest that PKA/cAMP as well as PDE7A is involved in regulation of cell proliferation and cytokine production of NKT cells, and that the inhibitory effects of ASB16165 in NKT cells shown here are mediated by increase in cellular cAMP level
Hong et al., J Surg Res 2010 : In this study, the role of PKA in mediating the effect of cAMP on NF-kappaB was investigated ... Overexpression of PKA increased IL-1beta plus IFNgamma induced NF-kappaB binding ... Overexpression of PKA increased IL-1beta plus IFNgamma induced NF-kappaB binding ... These results suggest that PKA is not essential for the inhibitory effect of cAMP on NF-kappaB binding activity in hepatocytes
Baum et al., Am J Physiol Endocrinol Metab 2009 : Glucagon alone had no effect on mTORC1 signaling but stimulated the activity of protein kinase A (PKA)
Kirschner et al., J Intern Med 2009 (Cell Transformation, Neoplastic...) : At the biochemical level, removal of Prkar1a from cells causes enhanced PKA activity, the same effect which has been observed in tumours isolated from CNC patients
Wall et al., Science signaling 2009 (Inflammation...) : Suppression of LPS induced TNF-alpha production in macrophages by cAMP is mediated by PKA-AKAP95-p105
Lyashkov et al., Am J Physiol Heart Circ Physiol 2009 (Calcium Signaling) : The purpose of the present study was to determine how ChR signaling initiated by a cholinergic agonist, carbachol ( CCh ), affects AC, cAMP, and PKA or sarcolemmal ion channels and LCRs and how these effects become integrated to generate the net response to a given intensity of ChR stimulation in single, isolated rabbit SANC ... At IC ( 50 ), CCh reduced cAMP and reduced PKA dependent phospholamban (PLB) phosphorylation by approximately 50 %
Cho et al., Inflamm Res 2009 : In view of the potential roles of PKA and MSK for MKP-1 induction and the ability of H89 to inhibit these kinases, this study examined the effect of H89 on MKP-1 induction by LPS and the role of cyclic-AMP response element binding protein ( CREB ) in the MKP-1 induction
Jiang et al., J Neuroimmunol 2009 : Pargyline stimulated CREB activation and Smac/DIABLO expression were prevented by the inhibitors of PKA , PLC and PKC ... Pargyline stimulated CREB activation and Smac/DIABLO expression were prevented by the inhibitors of PKA , PLC and PKC
Baker et al., Dev Biol 2009 : Phosphorylation and consequent stimulation of the tyrosine kinase c-Abl by PKA in mouse spermatozoa ; its implications during capacitation
Tobe et al., Cell division 2009 : It was found that Swe1 mediated inhibitory tyrosine phosphorylation of Cdc28 during filamentous growth is in part mediated by Ras2 activation of PKA , but not Kss1-MAPK, signaling
Almela et al., J Pharmacol Exp Ther 2009 (Substance Withdrawal Syndrome...) : The present findings demonstrate that the enhancement of ERK1/2 expression and the phosphorylation state of TH at Ser31 during morphine withdrawal are dependent on PKA and suggest cross-talk between PKA and ERK1/2 transduction pathway mediating morphine withdrawal induced activation ( phosphorylation ) of TH
Chaturvedi et al., J Biol Chem 2009 : Previously, we showed that interactions between p90 ( RSK1 ) ( RSK1 ) and the subunits of type I protein kinase A (PKA) regulate the activity of PKA and cellular distribution of active RSK1 ( Chaturvedi, D., Poppleton, H. M., Stringfield, T., Barbier, A., and Patel, T. B. ( 2006 ) Mol. Cell Biol. 26, 4586-4600 )
Xiong et al., J Biol Chem 2009 : We found that cAMP/protein kinase A (PKA) signaling is required for full vWF release
Nakao et al., J Dent Res 2009 (Alveolar Bone Loss...) : PTHrP induced Jagged1 up-regulation was mediated by PKA activation, but not by PKC
Vilas et al., Am J Physiol Cell Physiol 2009 (Epilepsy) : This indicates that PKA regulates WT and AE3 ( fl ) -A867D Cl ( - ) /HCO ( 3 ) ( - ) exchange activity
Uh et al., Reproductive biology and endocrinology : RB&E 2009 : Classically protein kinase A (PKA) and transcription factor activator protein 1 (AP-1) mediate the cyclic AMP ( cAMP ) induced-corticotrophin releasing hormone (CRH) expression in the placenta
Chaves et al., Mech Ageing Dev 2009 : The inhibitory effect of IL-10 on 20-49 years old group was reversed by the pre-treatment with H89 ( PKA inhibitor ) but not with PD169316 ( p38 MAPK inhibitor ) ... The inhibitory effect of IL-10 on 20-49 years old group was reversed by the pre-treatment with H89 ( PKA inhibitor ) but not with PD169316 ( p38 MAPK inhibitor )
Liu et al., Endocrine 2009 : Activation of TR4 orphan nuclear receptor gene promoter by cAMP/PKA and C/EBP signaling ... In this study, we found that cAMP/PKA , the mediator of fasting and glucagon signals, could induce TR4 gene expression that in turn modulates gluconeogenesis ... Mechanistic dissection by in vitro studies in hepatocytes demonstrated that cAMP/PKA might trigger C/EBP alpha and beta binding to the selective cAMP response element, which is located at the TR4 promoter, thus inducing TR4 transcription
Sun et al., Theoretical biology & medical modelling 2009 : It has also been found that the kinase PKA attenuates beta-catenin degradation
Murray et al., J Recept Signal Transduct Res 2009 : These findings indicate that cAMP/PKA serves as a point of convergence for regulation of IL-1alpha responses by multiple G ( s ) -coupled receptors and regulates IL-1alpha responses by a distinct mechanism that does not solely involve direct inhibition of p38, JNK, MEK or IKK
Friis et al., J Am Soc Nephrol 2009 : Taken together, our results show that a 5 to 7 % decrease in extracellular tonicity leads to AQP1 mediated water influx in JG cells, PLA(2)/COX-2 mediated prostaglandin dependent formation of cAMP, and activation of PKA , which promotes exocytosis of renin ... Taken together, our results show that a 5 to 7 % decrease in extracellular tonicity leads to AQP1 mediated water influx in JG cells, PLA(2)/COX-2 mediated prostaglandin dependent formation of cAMP, and activation of PKA , which promotes exocytosis of renin
Imanaga et al., Experimental and clinical cardiology 2004 : Activation of PKA promotes cell-to-cell coupling due to augmentation of the PKA mediated phosphorylation of Cx43 , with a rise in the quantity of and an increase in the expression of Cx43 ... A rise in the ionic strength of Ca ( 2+ ) and H ( + ) impaired cell communication, with the inhibition of PKA mediated Cx43 phosphorylation
Yan et al., Zhonghua Xue Ye Xue Za Zhi 2009 : To explore the regulatory role of protein kinase A (PKA) in platelet surface glycoprotein (GP) I balpha expression ... The actin binding protein (ABP) and talin proteolysis demonstrated that calpain was activated by PKA inhibitor and expressed on the platelet membrane
Hayashi et al., Thromb Haemost 2009 : These results suggested that cAMP elevating agents have two effects on PI3K signalling : inhibition of Akt phosphorylation independent of PKA ; and stimulation of GSK-3beta phosphorylation dependent on PKA
Liu et al., American journal of physiology. Renal physiology 2009 (Mechanotransduction, Cellular) : Interpretation of these results in light of the effects of these inhibitors on the flow induced elevation of [ Ca2+ ] ( i ) suggests that the principal cell apical BK channel is tonically inhibited by PKA and that flow stimulation of J ( K ) in the CCD is PKA and PKC dependent
Krintel et al., FEBS J 2009 : The exact molecular events of PKA mediated activation of HSL in vitro are yet to be determined, but increases in both Vmax and S0.5 seem to be involved, as recently shown for human HSL
Yum et al., Mol Cells 2009 : PKA mediated stabilization of FoxH1 negatively regulates ERalpha activity ... In the present study, we examined the effects of PKA activation on the ability of FoxH1 to represses ERalpha transcriptional activity ... We found that PKA increases the protein stability of FoxH1 , and that FoxH1 inhibits PKA induced and estradiol induced activation of an estrogen response element ( ERE ) ... These results suggest that PKA can negatively regulate ERalpha , at least in part, through FoxH1
Zhang et al., Mol Cell Biochem 2010 : Furthermore, disrupting PKA anchoring to AKAP or PKA activity significantly attenuated the PKA activity, VASP phosphorylation, and subsequent cell migration ... In conclusion, AKAP anchoring of PKA is an essential upstream event in regulation of PKA mediated VASP phosphorylation and subsequent endothelial cell migration, which contributes to explore new methods for controlling endothelial cell migration related diseases and angiogenesis
Vuchak et al., Mol Pharmacol 2009 (MAP Kinase Signaling System) : By contrast, PKA activity was required for cAMP stimulated ERK phosphorylation and was sufficient to increase ERK phosphorylation in starved cells
Bogatcheva et al., J Cell Physiol 2009 : Both VASP and filamin1 phosphorylation was significantly increased as a result of PKA activation ... Both VASP and filamin1 phosphorylation was significantly increased as a result of PKA activation ... Our data indicate that while PKA dependent VASP phosphorylation contributes to the protective effect of cAMP elicited on LPS compromised monolayers, filamin1 phosphorylation is unlikely to play a significant role in this process
Mehebik-Mojaat et al., J Cell Biochem 2009 (Insulin Resistance) : When leptin signaling was blocked by PKA inhibitors, insulin induced NOS activity and NOS III phosphorylation at Ser ( 1179 ) was observed ... In the presence of leptin and insulin, ( i ) IRS-1 was phosphorylated on Ser ( 307 ) and this effect was prevented by PKA inhibitors, ( ii ) JAK-2 was dephosphorylated, an effect prevented by SHP-1 inhibitor
Lee et al., Mol Cell Neurosci 2009 : PKA inhibitor effectively inhibited VASP phosphorylation and chemotaxis, indicating that P2Y12 mediated activation of PKA and subsequent VASP phosphorylation are involved in the regulation of microglia chemotaxis ... PKA inhibitor effectively inhibited VASP phosphorylation and chemotaxis, indicating that P2Y12 mediated activation of PKA and subsequent VASP phosphorylation are involved in the regulation of microglia chemotaxis
Ding et al., Am J Physiol Cell Physiol 2009 : In contrast, in the absence of GPR30 expression in cultured VSMC, estradiol stimulated PKA activity and inhibited ERK phosphorylation ... Thus the effect of estradiol on vascular smooth muscle cell apoptosis is likely dependent on the balance between ER-mediated PKA activation and GPR30 mediated PKA inhibition and PI3 kinase activation
Tai et al., J Biol Chem 2009 (Carcinoma, Hepatocellular) : These data suggest that C/EBPbeta is involved in the DHA regulated increase in SAA1 expression via PKA dependent mechanisms ... These data suggest that C/EBPbeta is involved in the DHA regulated increase in SAA1 expression via PKA dependent mechanisms
Chu et al., Chin J Physiol 2009 (Heart Diseases) : Moreover, immunoprecipitation assay revealed that treatment with IGF-II could enhance the interaction of IGF2R with Galphai and Galphaq but reduce its binding with Galphas, resulting in the reduction of phospho-PKA and the activation of PLC-beta
Spirli et al., Gastroenterology 2010 (Cysts...) : Inhibition of protein kinase A (PKA) reduced VEGF secretion and pERK1/2 expression ... Inhibition of protein kinase A (PKA) reduced VEGF secretion and pERK1/2 expression ... In Pkd2-defective LCECs, PKA dependent ERK1/2 signaling controls HIF-1alpha dependent VEGF secretion and VEGFR-2 signaling
Jeske et al., Pain 2009 (Calcium Signaling) : Recent studies have reported that the A-kinase Anchoring Protein 150 ( AKAP150 ) mediates PKA phosphorylation of TRPV1 in several nociceptive models
Blaker et al., Arterioscler Thromb Vasc Biol 2009 : PKA dependent phosphorylation of serum response factor inhibits smooth muscle-specific gene expression
Morimoto et al., Biochem Biophys Res Commun 2009 : The Ca ( 2+ ) leak in isoproterenol treated preparations was significantly increased when the PKA dependent phosphorylation of RyR was increased without the involvement of CaMKII dependent phosphorylation ... Both the increase in Ca ( 2+ ) leak and the phosphorylation of RyR were blocked by a PKA inhibitor ... Our results show that beta-adrenergic stimulation increases Ca ( 2+ ) leak from SR through PKA dependent phosphorylation of RyR
Wang et al., Cell Res 2009 (Neoplasms) : Importantly, PKA mediated phosphorylation of ezrin promotes the NK cell internalization process
Baviera et al., Mol Cell Endocrinol 2010 : On the other hand, inhibition of PKA by H89 further increased the phosphorylation levels of AKT and Foxo3a induced by epinephrine, DBcAMP or 8CPT-2Me-cAMP ... On the other hand, inhibition of PKA by H89 further increased the phosphorylation levels of AKT and Foxo3a induced by epinephrine, DBcAMP or 8CPT-2Me-cAMP
Gao et al., J Biol Chem 2009 : RSK1 attenuated the ability of cAMP to activate PKA in vitro and this modulation was abrogated by peptide E, but not by peptide A ... These findings demonstrate that interactions between endogenous RSK1 and PKAc in intact cells regulate the ability of cAMP to activate PKA and identify a novel mechanism by which PKA activity is regulated by the Erk1/2 pathway
Zhong et al., Am J Physiol Regul Integr Comp Physiol 2009 (Disease Models, Animal...) : CGRP ( 8-37 ), a selective CGRP receptor antagonist, RP67580, a selective neurokinin-1 receptor antagonist, PKC-epsilon V1-2, a selective PKC-epsilon inhibitor, or H-89, a selective PKA inhibitor , abolished SLIGRL protection by inhibiting the recovery of the rate of coronary flow, maximum rate of left ventricular pressure development, and left ventricular developed pressure, and increasing left ventricular end-diastolic pressure in WT but not TRPV1 ( -/- ) hearts
Bersimbaev et al., Fiziol Zh SSSR Im I M Sechenova 1990 (Second Messenger Systems) : Like TPA, both C and CCK , but not H, stimulated the Ca+2-sensitive particulate PK C. H increased the activity of cAMP dependent PK A
Xia et al., Mol Cell Biol 2010 : Interestingly, the activation of PI3K/Akt appeared to be independent of the activation of PKA, whereas both PI3K/Akt and PKA signaling inactivated GSK-3 and increased beta-catenin translocation
Skoblenick et al., J Mol Neurosci 2010 : Protein kinase A (PKA) participation in the signaling pathway was examined using selective PKA inhibitors, which reduced synapsin II expression in cell cultures while dopaminergic agents were unable to increase synapsin II in the presence of the PKA inhibitor
Vega et al., Circulation. Arrhythmia and electrophysiology 2009 (Andersen Syndrome...) : This PKA simulated catecholaminergic stimulation caused marked reduction of outward I(K1) compared with Kir2.1-WT. PKA induced reduction in I(K1) was eliminated by mutating the phosphorylation site at serine 425 ( S425N ) ... Heteromeric Kir2.1-V227F and WT channels showed an unusual latent loss of function biophysical phenotype that depended on PKA dependent Kir2.1 phosphorylation
Sánchez-Muñoz et al., J Neurosci Res 2010 : Here we show that the early activation of CREB was dependent on cAMP/PKA along with CaMKs pathways whereas the ERK/MAPK and CaMKs were implicated in the second peak
Reddy et al., J Membr Biol 2009 : PKA mediates constitutive activation of CFTR in human sweat duct
He et al., Am J Physiol Heart Circ Physiol 2010 : PKA , Rap1, ERK1/2, and p90RSK mediate PGE2 and EP4 signaling in neonatal ventricular myocytes ... Treatment of NVMs with PGE ( 2 ) activated Rap , and this activation was inhibited in part by an EP4 antagonist and PKA inhibition ... PGE ( 2 ) stimulation of c-Fos was dependent on EP4, PKA , ERK1/2, and p90RSK, whereas only the latter two kinases were involved in PGE ( 2 ) regulation of early growth response-1
Ogrodnik et al., J Physiol 2010 (Calcium Signaling) : This was likely to have been mediated by Ca ( 2+ ) /calmodulin dependent protein kinase ( CaMKII ), rather than cAMP dependent protein kinase (PKA)
Nemani et al., Arch Biochem Biophys 1991 : We have adapted this method to study the effect of PTH on PKA and PKC activity using thin slices of rat renal cortex ... PTH ( 250 nM ) stimulated cytosolic PKA activity four- to fivefold within 30 s, and PKA activity was sustained for at least 5 min. PTH also rapidly stimulated PKC activity in the membrane fraction and decreased PKC activity in the cytosol
Bradley et al., J Biol Chem 2010 : The aim of this study was to examine if PKA dependent phosphorylation of VACM-1/Cul5 controls its neddylation status, phosphorylation by PKC, and ultimately growth ... Western blot analysis of cell lysates immunoprecipitated with an antibody directed against a PKA-specific phosphorylation site and probed with anti-VACM-1-specific antibody showed that PKA dependent phosphorylation of VACM-1 protein was decreased in cells transfected with ( S730A ) VACM-1 cDNA when compared with the cytomegalovirus transfected cells
Matavel et al., Channels (Austin) 2010 (Ion Channel Gating...) : These regulatory pathways were shown to crosstalk, with PKA phosphorylation increasing the apparent affinity of IKs to PIP ( 2 )
Bobick et al., J Cell Biochem 2010 : Depleted cellular vimentin also induced a drastic reduction in PKA phosphorylation levels but did not affect the phosphorylation of multiple other chondro-regulatory kinases and transcription factors, including ERK1/2, p38, Smad2, and Smad1/5/8
Djouder et al., EMBO J 2010 : TAG resynthesis is associated with high-energy consumption, which when inordinate, leads to increased AMPK activity that acts to restrain hydrolysis of TAGs by inhibiting PKA mediated activation of HSL ... These results suggest a new mechanism of negative regulation of AMPK activity by PKA that is important for converting a lipolytic signal into an effective lipolytic response
Kang et al., The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 2008 : Taken together, the results suggest that the stimulation of reverse NCX1 by STS is independent of PKC and/or PKA inhibition
Bidwell et al., Prostaglandins Other Lipid Mediat 2010 (Glioma...) : Prostaglandin E2 activates cAMP response element binding protein in glioma cells via a signaling pathway involving PKA dependent inhibition of ERK ... Inhibition of PKA by H-89 or expression of a dominant negative PKA construct attenuated PGE ( 2 ) -induced CREB activation
Oh et al., Carcinogenesis 2010 (Carcinoma, Squamous Cell...) : The E5-induced decrease in Bax expression was inhibited by a cyclooxygenase-2 (COX-2) inhibitor, prostaglandin E2 ( PGE ( 2 ) ) receptor antagonists and cyclic adenosine monophosphate dependent protein kinase (PKA) inhibitor
Barzi et al., J Cell Sci 2010 : Cerebellar granular neuronal precursors ( CGNPs ) proliferate in response to the mitogenic activity of Sonic hedgehog (Shh) , and this proliferation is negatively regulated by activation of cAMP dependent protein kinase (PKA) ... Here we report that, in the presence of Shh, inactive C-PKA accumulates at the cilium base of proliferative CGNPs whereas removal of Shh triggers the activation of PKA at this particular location
Liu et al., Traffic 2010 (Hyperalgesia) : Inhibition of PKA completely blocked the PGE ( 2 ) -promoted effect on Na(v)1.8
Chen et al., Mol Cells 2010 (Calcium Signaling) : Previous studies indicated that intracellular calcium, PKA , and MAPKs are involved in CREB phosphorylation and activation
Yamashita et al., Journal of ovarian research 2009 : Activation of PKA , p38 MAPK and ERK1/2 by gonadotropins in cumulus cells is critical for induction of EGF-like factor and TACE/ADAM17 gene expression during in vitro maturation of porcine COCs ... The results showed that PKA , p38 MAPK and ERK1/2 positively controlled the expression of EGF-like factor and TACE/ADMA17 , the latter of which impacts the cumulus expansion and oocyte maturation of porcine COCs via the EGFR-ERK1/2 pathway in cumulus cells
Zou et al., Acta Pharmacol Sin 2010 (Glomerulonephritis) : The PPARgamma agonists also inhibited alpha-SMA and collagen IV protein expression by blocking PKA activation ... The PPARgamma agonists also inhibited alpha-SMA and collagen IV protein expression by blocking PKA activation
Hu et al., Acta Pharmacol Sin 2010 (Pancreatic Neoplasms) : Activation of beta-AR receptor transactivates epidermal growth factor receptor (EGFR) and then elicits Akt and ERK1/2 in a PKA dependent manner, which together up-regulate levels of HIF-1alpha and downstream target genes independently of oxygen level ... Activation of beta-AR receptor transactivates epidermal growth factor receptor (EGFR) and then elicits Akt and ERK1/2 in a PKA dependent manner, which together up-regulate levels of HIF-1alpha and downstream target genes independently of oxygen level
Lin et al., Mol Cell Neurosci 2010 : We found that while interaction between Kv4.2 and KChIP4a does not require PKA phosphorylation of Kv4.2 ( S552 ), phosphorylation of this site is necessary for both enhanced stabilization and membrane expression of Kv4.2 channel complexes produced by KChIP4a
Belleannée et al., Am J Physiol Cell Physiol 2010 : The PKA inhibitor myristoylated protein kinase inhibitor ( mPKI ) inhibited adenosine dependent V-ATPase apical accumulation, indicating the participation of the P1 A2B receptor
Kooij et al., J Mol Cell Cardiol 2010 : Protein kinase A (PKA) mediated phosphorylation of Ser23/24 of cardiac troponin I (cTnI) causes a reduction in Ca ( 2+ ) -sensitivity of force development
Dodge-Kafka et al., J Biol Chem 2010 : Accordingly, expression of a B56delta mutant that can not be phosphorylated by PKA results in increased PDE4D3 phosphorylation
Goel et al., American journal of physiology. Renal physiology 2010 : AVP- and forskolin induced translocation of TRPC3 in IMCD-3 cells was also blocked by two additional inhibitors of PKA , specifically Rp-cAMPS and the myristoylated inhibitor of PKA ( m-PKI )
Kroviarski et al., FASEB J 2010 : Here we report that NOX1 activity is regulated through MAP kinase ( MAPK ), protein kinase C ( PKC ), and protein kinase A (PKA) dependent phosphorylation of NOXA1
Spirli et al., Hepatology 2010 (Cysts...) : The expression of insulin-like growth factor 1 (IGF1), insulin-like growth factor 1 receptor ( IGF1R ), and phosphorylated mammalian target of rapamycin ( p-mTOR ) and the protein kinase A (PKA) dependent phosphorylation of extracellular signal regulated kinase 1/2 ( ERK1/2 ) are also up-regulated in LCE ... CONCLUSION : These data demonstrate that PKA dependent up-regulation of mTOR has a central role in the proliferative, antiapoptotic, and pro-angiogenic effects of IGF1 and VEGF in polycystin-2-defective mice
Stetler et al., Proc Natl Acad Sci U S A 2010 (Brain Ischemia) : Induction of APE1 expression requires PKA- and p38 dependent phosphorylation of cAMP response-element binding and activating transcription factor 2, which leads to transactivation of the APE1 promoter
Guo et al., Cell Metab 2010 : In cultured MC3T3E1 preosteoblastic cells, PTH dramatically suppressed Dkk1 expression, induced PKA mediated phosphorylation of beta-catenin , and significantly enhanced Lef1 expression
Gong et al., American journal of physiology. Renal physiology 2010 : Vacuolar H+-ATPase apical accumulation in kidney intercalated cells is regulated by PKA and AMP activated protein kinase ... In developmentally related epididymal clear cells, we showed previously that PKA , acting downstream of soluble adenylyl cyclase (sAC), induces V-ATPase apical membrane accumulation ... Here, we examined the regulation of V-ATPase subcellular localization in intercalated cells by PKA and AMPK in rat kidney tissue slices ex vivo
Lara et al., Arch Biochem Biophys 2010 : Cholera toxin, an activator of Gs protein, mimicked it ; ( 2 ) in the presence of Ang II, Ang- ( 1-7 ) increased the PKA activity 10-fold ; ( 3 ) the peptide inhibitor of PKA blocked the Ang- ( 1-7 ) effect on Ang II-stimulated Na+-ATPase ; ( 4 ) Ang- ( 1-7 ) reverses the Ang II-stimulated PKC activity ; ( 5 ) cAMP mimicked the Ang- ( 1-7 ) effect on the Ang II-stimulated Na+-ATPase ... Cholera toxin, an activator of Gs protein, mimicked it ; ( 2 ) in the presence of Ang II, Ang- ( 1-7 ) increased the PKA activity 10-fold ; ( 3 ) the peptide inhibitor of PKA blocked the Ang- ( 1-7 ) effect on Ang II-stimulated Na+-ATPase ; ( 4 ) Ang- ( 1-7 ) reverses the Ang II-stimulated PKC activity ; ( 5 ) cAMP mimicked the Ang- ( 1-7 ) effect on the Ang II-stimulated Na+-ATPase ... Cholera toxin, an activator of Gs protein, mimicked it ; ( 2 ) in the presence of Ang II , Ang- ( 1-7 ) increased the PKA activity 10-fold ; ( 3 ) the peptide inhibitor of PKA blocked the Ang- ( 1-7 ) effect on Ang II-stimulated Na+-ATPase ; ( 4 ) Ang- ( 1-7 ) reverses the Ang II-stimulated PKC activity ; ( 5 ) cAMP mimicked the Ang- ( 1-7 ) effect on the Ang II-stimulated Na+-ATPase
Glausier et al., Neuroscience 2010 : Inhibition of PP1 occurs through PKA mediated phosphorylation of dopamine- and cAMP regulated phosphoprotein 32 kDa ( DARPP-32 ) or the related protein inhibitor-1 ( I-1 ), and the availability of DARPP-32 is essential to the functional outcome of D1R activation in the basal ganglia
Shi et al., J Alzheimers Dis 2010 (Alzheimer Disease) : Ginsenoside Rg1 attenuates amyloid-beta content, regulates PKA/CREB activity, and improves cognitive performance in SAMP8 mice
Zhou et al., Proc Natl Acad Sci U S A 2010 : PKC phosphorylation at S ( 1151 ) also influences stimulation of BK channel activity by protein kinase G ( PKG ) and protein kinase A (PKA)
Wang et al., FASEB J 2010 : The binding is required to induce G-protein/cAMP/protein kinase A (PKA) signaling, which controls PKA dependent phosphorylation of GluR1 and beta ( 2 ) AR, and AMPA receptor mediated excitatory postsynaptic currents ( EPSCs )
Tao et al., Journal of molecular signaling 2010 : AKAR2-AKAP12 can be expressed in mammalian cells, is fully functional, and reveals the spatial-temporal activation of AKAP12 undergoing phosphorylation by PKA in response to beta-adrenergic activation in human epidermoid carcinoma A431 cells
Amente et al., Oncogene 2010 : In addition, we demonstrate that Myc binds and recruits LSD1 to the E-box chromatin and the formation of this complex is stimulated by cAMP-PKA
Yao et al., Neuroscience 2010 (Calcium Signaling) : Acute Ca ( 2+ ) -dependent desensitization of 5-HT(1A) receptors is mediated by activation of protein kinase A (PKA) in rat serotonergic neurons
Guo et al., Circ Res 2010 (Calcium Signaling) : PKA dependent RyR2 phosphorylation has no significant effect on binding of either FKBP12 or 12.6 to RyR2 in myocytes
Miyoshi et al., Mol Cell Endocrinol 2010 : Furthermore, in the presence of oocytes, the inhibition of endogenous FGF receptor signaling suppressed FSH- and forskolin induced progesterone and cAMP, showing that endogenous FGF system is involved in activation of FSH induced cAMP-PKA signaling via ERK and SAPK/JNK
Oeseburg et al., Arterioscler Thromb Vasc Biol 2010 (Diabetes Mellitus...) : Further analysis revealed that GLP-1 activates the cAMP response element binding ( CREB ) transcription factor in a cAMP/protein kinase A (PKA) dependent manner, and inhibition of the cAMP/PKA pathway abolished the GLP-1 protective effect
Kirschner et al., Mol Cell Endocrinol 2010 (Acromegaly...) : Loss of PRKAR1A causes enhanced PKA signaling, which leads to pituitary tumorigenesis
Solc et al., Mol Hum Reprod 2010 : In addition, PKA mediated phosphorylation of the phosphatase CDC25B results in its cytoplasmic retention
Wang et al., Am J Physiol Cell Physiol 2010 : Simultaneous inhibition of PKA and PI3K reduces IL-6 expression in stimulated chondrocytes well below the basal levels of untreated cells
Sakakibara et al., Biosci Biotechnol Biochem 2010 : The inhibitors of cAMP dependent protein kinase (PKA) and AMP activated protein kinase (AMPK) blocked acetate induced eNOS phosphorylation in the early and the late phase respectively
Chen et al., Am J Physiol Cell Physiol 2010 : The cAMP antagonist and the PKA inhibitors reduced NGF induced p35 expression
Uusi-Oukari et al., Neurochem Int 2010 : While JNK activity negatively regulates delta subunit mRNA expression in unstimulated neurons, activity of ERK1/2 and PKA are required for full induction of GABA(A) receptor delta subunit expression in response to AMPA receptor stimulation
Hnisz et al., PLoS Pathog 2010 (Candidiasis) : The Set3/Hos2 histone deacetylase complex attenuates cAMP/PKA signaling to regulate morphogenesis and virulence of Candida albicans ... The Set3/Hos2 histone deacetylase complex attenuates cAMP/PKA signaling to regulate morphogenesis and virulence of Candida albicans
Qu et al., Free Radic Biol Med 2010 : It was demonstrated that the H ( 2 ) O ( 2 ) -induced up-regulation of Mipu1 in H9c2 cardiomyocytes was mediated by cAMP/PKA dependent CREB activation
Sántha et al., Acta Physiol Hung 2010 : Activation of the cannabinoid 1 (CB1) receptor that is expressed by the majority of the TRPV1 expressing primary sensory neurons reduces PKA activity ... These findings suggest that activation of the CB1 receptor can reduce the activity of TRPV1 in primary sensory neurons, and that this inhibitory effect could be mediated through the reduction of PKA mediated phosphorylation of TRPV1
Alzamora et al., J Biol Chem 2010 : Finally, Ser-175 was necessary for PKA stimulated apical accumulation of the V-ATPase in a polarized rabbit cell line of collecting duct A-type intercalated cell characteristics ( Clone C )
Gao et al., J Biol Chem 2010 (Breast Neoplasms) : In this study, we have demonstrated the effect of PKA on NF-kappaB activity in view of AKIP1 action ; and in 293 and HeLa cells, where the endogenous AKIP1 expression is minimal, PKA activating agents inhibited the NF-kappaB dependent reporter gene expression, blocked the interaction of PKAc and p65 subunit of NF-kappaB, and attenuated PKA dependent phosphorylation of p65 on Ser-276 ... In this study, we have demonstrated the effect of PKA on NF-kappaB activity in view of AKIP1 action ; and in 293 and HeLa cells, where the endogenous AKIP1 expression is minimal, PKA activating agents inhibited the NF-kappaB dependent reporter gene expression, blocked the interaction of PKAc and p65 subunit of NF-kappaB, and attenuated PKA dependent phosphorylation of p65 on Ser-276
Glaser et al., Hepatology 2010 (Cholestasis, Extrahepatic...) : In vitro, secretin increased proliferation, PKA activity, and ERK1/2 phosphorylation of large cholangiocytes that was blocked by PKA and mitogen activated protein kinase kinase inhibitors
Daniel et al., Dev Biol 2010 : Moreover, we show here that PKA and SRC family are involved in the activation of EGFR by 10 microM ouabain, further demonstrating that ouabain induces the acrosome reaction by a mechanism mediated by the trans-activation of EGFR
Dechant et al., EMBO J 2010 (Second Messenger Systems) : Finally, V-ATPase is also regulated by glucose in the Min6 beta-cell line and contributes to PKA activation and insulin secretion
Duquesnes et al., Pflugers Arch 2010 : Interestingly, inhibition of PKA partly suppressed both Iso induced increases in Cx43 phosphorylation and in GJIC
Ketsawatsomkron et al., Vascul Pharmacol 2010 (Insulin Resistance) : Finally, we also showed that Ang II-induced activation of PTP-1B in VSMC was PKA/JAK2 dependent ... Finally, we also showed that Ang II-induced activation of PTP-1B in VSMC was PKA/JAK2 dependent
Barinov et al., Klin Lab Diagn 2010 (Diabetic Foot) : In patients with DFS, the condition for wound process termination was decreased baseline iNOS activity and enhanced arginase-1 activity during PKA stimulation with the lower activity of 5-LO , PDE, and PKS
Liu et al., J Cardiovasc Pharmacol 2010 : PKA-specific activation by PKA agonist enhanced the upregulation of ABCA1 serine phosphorylation and ABCA1 mediated cholesterol efflux by D4-F
Valbuena et al., PloS one 2010 : We conclude that both the PKA and the TOR pathways inhibit Ste11 nuclear accumulation to repress Ste11 dependent gene expression
Kim et al., Biochem Biophys Res Commun 2010 : Here we showed that cAMP induced activation of protein kinase A (PKA) and Akt is essential for the transcriptional activation of PPAR-gamma ... In addition, IBMX induced PPAR-gamma transcriptional activity was blocked by PI3K/Akt, PKA , or Rap1 inhibitors
Kostenko et al., Cell Mol Life Sci 2011 : We identified noroxoaconitine as an ATP competitor that inhibited the catalytic activity of MK5 in vitro ( IC50 = 37.5 µM ; K(i) = 0.675 µM ) and prevented PKA induced nuclear export of MK5, a process that depends on kinase active MK5
Liu et al., J Mol Neurosci 2011 : To further understand the anti-inflammatory effect of adenosine cyclic 3',5'-monophosphate ( cAMP ), we examined the effect of protein kinase A (PKA) and cAMP-responsive guanine nucleotide exchange factor ( Epac ) on the transcription and production of cytokines and on the activity of mitogen activated protein kinases ( MAPK ) p38 and glycogen synthase kinase-3ß ( GSK-3ß ) in endotoxin treated rat primary cultured microglia ... To further understand the anti-inflammatory effect of adenosine cyclic 3',5'-monophosphate ( cAMP ), we examined the effect of protein kinase A (PKA) and cAMP-responsive guanine nucleotide exchange factor ( Epac ) on the transcription and production of cytokines and on the activity of mitogen activated protein kinases ( MAPK ) p38 and glycogen synthase kinase-3ß ( GSK-3ß ) in endotoxin treated rat primary cultured microglia
Oliveira et al., PloS one 2010 : Simulations further demonstrate that generation of the cAMP microdomain requires a pool of PDE4D anchored in the cytosol and also requires PKA mediated phosphorylation of PDE4D which increases its activity
Dankel et al., Biochim Biophys Acta 2010 : We have therefore investigated the role of cAMP/PKA in regulation of HNF-4alpha in COS-1 cells and the hepatoma HepG2 cell line ... cAMP/PKA inhibited the transcriptional activity of HNF-4alpha in COS-1 cells, whereas a stimulatory effect was observed in HepG2 cells ... The cAMP induced inhibition of HNF-4alpha in COS-1 cells was counteracted by overexpression of the nuclear receptor coactivator PGC-1alpha, and cAMP/PKA dependent induction of the PGC1A gene in HepG2 cells seems to explain the cell specific differences ... Similar to the cAMP/PKA mediated regulation of HNF-4alpha , overexpression of the cAMP-response element binding protein ( CREB ) inhibited the transcriptional activity of HNF-4alpha in COS-1 cells, regardless of cAMP/PKA activation and CREB phosphorylation ... Moreover, activation of CREB by cAMP/PKA further stimulated HNF-4alpha transactivation in HepG2 cells ... Moreover, activation of CREB by cAMP/PKA further stimulated HNF-4alpha transactivation in HepG2 cells ... In conclusion, our results suggest that the level of PGC-1alpha determines whether the cAMP/PKA-pathway overall stimulates or inhibits HNF-4alpha transcriptional activation
Han et al., Toxicol Lett 2010 (Breast Neoplasms) : o, p'-DDT also increased the phosphorylation of PKA , Akt, ERK, and JNK in their signaling pathways in MCF-7 and MDA-MB-231 cells
Nakaya et al., Atherosclerosis 2010 : Cilostazol did not change intracellular cAMP levels in THP-1 macrophages and RAW264.7 cells, and a protein kinase A (PKA) inhibitor did not affect cilostazol induced ABCA1 and ABCG1 expression ... Cilostazol did not change intracellular cAMP levels in THP-1 macrophages and RAW264.7 cells, and a protein kinase A (PKA) inhibitor did not affect cilostazol induced ABCA1 and ABCG1 expression
Dong et al., Neurosci Lett 2010 : These results suggest PACAP to be an upstream regulator for inducing Pdyn mRNA expression through PKA
Yao et al., Nat Med 2010 (Cocaine-Related Disorders...) : PKA and PKC phosphorylate and activate tyrosine hydroxylase , further increasing dopamine synthesis in a positive-feedback loop
Choi et al., Mol Cell Biol 2010 (Insulin Resistance) : Furthermore, insulin regulates total PKA activity in an Akt dependent manner ... Furthermore, insulin regulates total PKA activity in an Akt dependent manner
Roscioni et al., Br J Pharmacol 2011 : Activation of both Epac and PKA similarly prevented PDGF induced phenotype switching and PDGF induced activation of ERK ... Interestingly, only PKA activation resulted in inhibition of PDGF induced phosphorylation of p70 ( S6K )
Yan et al., FASEB J 2011 : The antimitogenic efficacy of PGE ( 2 ) in inhibiting control cultures was associated with greater ability to stimulate sustained PKA activation and greater inhibition of late-phase promitogenic p42/p44 and PI3K activities ... The antimitogenic efficacy of PGE ( 2 ) in inhibiting control cultures was associated with greater ability to stimulate sustained PKA activation and greater inhibition of late-phase promitogenic p42/p44 and PI3K activities ... The antimitogenic efficacy of PGE ( 2 ) in inhibiting control cultures was associated with greater ability to stimulate sustained PKA activation and greater inhibition of late-phase promitogenic p42/p44 and PI3K activities
Melnikova et al., PloS one 2010 (Disease Progression...) : Herein, we demonstrate that both inhibition of PKA dependent CREB phosphorylation, as well as silencing of CREB expression by shRNA, restored AP-2alpha protein expression in two metastatic melanoma cell lines
Santini et al., PloS one 2010 (Dyskinesias...) : We here studied, in the gold-standard non-human primate model of Parkinson 's disease, the changes in PKA dependent phosphorylation of DARPP-32 and GluR1 AMPA receptor, as well as in ERK and ribosomal protein S6 ( S6 ) phosphorylation, associated to acute and chronic administration of L-DOPA ... We here studied, in the gold-standard non-human primate model of Parkinson 's disease, the changes in PKA dependent phosphorylation of DARPP-32 and GluR1 AMPA receptor, as well as in ERK and ribosomal protein S6 ( S6 ) phosphorylation, associated to acute and chronic administration of L-DOPA ... We here studied, in the gold-standard non-human primate model of Parkinson 's disease, the changes in PKA dependent phosphorylation of DARPP-32 and GluR1 AMPA receptor, as well as in ERK and ribosomal protein S6 ( S6 ) phosphorylation, associated to acute and chronic administration of L-DOPA
Zhang et al., J Biol Chem 2010 : These results suggest that activation of SHIP1 by PKA via phosphorylation on Ser ( 440 ) is an important regulatory event in hematopoietic cells
Tee et al., Dev Biol 2010 : We also found that BMP2 was higher in unbudded regions of the GDNF stimulated WD. Treatment of isolated WDs with BMP2 suppressed budding and resulted in a 3-fold increase in PKA activity
Tumati et al., Eur J Pharmacol 2010 (Pain) : In the present work we investigated the hypothesis that morphine pretreatment also sensitizes ACs toward Gs-protein coupled excitatory modulators ( such as PGE2 ), leading to augmented PKA dependent CGRP release from PGE2 stimulated primary sensory dorsal root ganglion ( DRG ) neurons ... Our results show that sustained morphine treatment potentiated PGE2 mediated cAMP formation and augmented PGE2 evoked CGRP release from cultured primary sensory neurons in a PKA dependent manner
Jin et al., Blood 2010 : We show here that Gs-linked G protein coupled receptor activation of cAMP dependent protein kinase (PKA) plays an important role in regulating the switch between endothelial cell adhesion and migration by activating C-terminal Src kinase, leading to inhibition of pp60Src ... Activated PKA blocks pp60Src dependent vascular endot helial-cadherin phosphorylation, thereby stimulating cell-cell adhesion while suppressing endothelial cell polarization, motility, angiogenesis, and vascular permeability
Song et al., J Biol Chem 2010 : We found that Ser-21/9 phosphorylation of GSK-3 was mediated by Ca ( 2+ ) /calmodulin dependent protein kinase II ( CaMKII ) but not by Akt/PKB, PKA , or p90 ( RSK )
Lin et al., Nucleic Acids Res 2011 : Here we show that the interaction of GCM1 and dual-specificity phosphatase 23 (DUSP23) is enhanced by PKA dependent phosphorylation of GCM1 on Ser269 and Ser275
Toranzo et al., Zygote 2011 : Our results suggest that in incompetent oocytes of B. arenarum two signal transduction pathways may be involved in the control of MPF activation : ( 1 ) the inhibition of phosphatase 2A that through the MEK-MAPK pathway regulates the activity of the Myt1 ; and ( 2 ) the inhibition of AMPc-PKA , which affects the activity of the Cdc25 phosphatase
Soltis et al., Biophys J 2010 (Arrhythmias, Cardiac...) : Cardiac excitation-contraction coupling is a highly coordinated process that is controlled by protein kinase signaling pathways, including Ca ( 2+ ) /calmodulin dependent protein kinase II ( CaMKII ) and protein kinase A (PKA) ... To better understand mechanisms of cardiac CaMKII function, we integrated dynamic CaMKII dependent regulation of key Ca ( 2+ ) handling targets with previously validated models of cardiac EC coupling, Ca ( 2+ ) /calmodulin dependent activation of CaMKII, and ß-adrenergic activation of PKA
Wu et al., J Mol Cell Cardiol 2011 : These results demonstrate that FN acts via a5ß1 integrin to increase force production in myocardium and that this effect is partly mediated by increases in [ Ca ( 2+ ) ] ( i ) and Ca ( 2+ ) sensitivity, PKA activation and phosphorylation of phospholamban
Almeida et al., Hum Mol Genet 2011 (Cryopyrin-Associated Periodic Syndromes) : NOMID tumor cells showed high PKA activity, and an increase in their cAMP signaling led to PKA-specific activation of caspase-1 ... Increased PKA led to inflammation independent activation of caspase-1 via over-expression of the proto-oncogene ( and early osteoblast factor ) Ets-1
Yang et al., Br J Pharmacol 2011 (Arrhythmias, Cardiac) : Urocortin 2 enhanced contractility in mouse ventricular myocytes via activation of CRF2 receptors in a cAMP/PKA- and Ca² ( + ) /CaMKII dependent manner
Lo et al., J Orthop Res 2011 : By using different target-specific cAMP analogs : 8-CPT-2Me-cAMP which specifically activates exchange protein activated by cAMP ( Epac ), and 6-Bnz-cAMP which specifically activates protein kinase A (PKA) , we observed that the PKA signaling pathway plays a dominant role in this process
Raghuraman et al., J Neurochem 2010 : PKA inhibitor restored GAD67 activity and GABA levels in IH treated cells ... These results provide evidence for the role of D1R-cAMP-PKA signaling in IH-mediated inhibition of GAD67 via protein phosphorylation resulting in down-regulation of GABA synthesis
Makarevich et al., Horm Metab Res 2010 : A PKA inhibitor, KT 5720 suppressed the expression of PKA and MAPK/ERK1,2 , the IGF-I release, and the ratio of PCNA positive cells in granulosa cells ... A PKA inhibitor, KT 5720 suppressed the expression of PKA and MAPK/ERK1,2 , the IGF-I release, and the ratio of PCNA positive cells in granulosa cells ... A PKA inhibitor, KT 5720 suppressed the expression of PKA and MAPK/ERK1,2, the IGF-I release, and the ratio of PCNA positive cells in granulosa cells ... These observations confirm the involvement of PKs in control of basic ovarian functions and demonstrate the involvement of PKA in stimulation of ovarian cell proliferation and MAPK ( but not CDK ) and in promotion of ovarian IGF-I release ... These observations confirm the involvement of PKs in control of basic ovarian functions and demonstrate the involvement of PKA in stimulation of ovarian cell proliferation and MAPK ( but not CDK ) and in promotion of ovarian IGF-I release
Xie et al., Journal of molecular cell biology 2010 : Here, we show that PKA activation also leads to increased steady-state RGS13 expression through RGS13 phosphorylation, which inhibits RGS13 protein degradation
Gaudy et al., J Biol Chem 2010 : In gain-of-function studies, adenoviral expression of SOCS3 in primary hepatocytes caused a 50 % decrease in 8-br-cAMP dependent PKA phosphorylation of the transcription factor CREB
Lajevic et al., Immunology 2011 (Thymoma) : p38 MAPK activation involves the ßAR, Gs protein, AC, cAMP and PKA , as determined through the use of a ßAR antagonist, activators of AC and cAMP, and S49 clonal mutants deficient in Gs and PKA ... p38 MAPK activation involves the ßAR, Gs protein, AC, cAMP and PKA , as determined through the use of a ßAR antagonist, activators of AC and cAMP, and S49 clonal mutants deficient in Gs and PKA
Bouley et al., J Am Soc Nephrol 2011 : Pharmacologic inhibition of protein kinase A (PKA) and deficiency of a critical PKA phosphorylation site on AQP2 both prevented calcitonin induced membrane accumulation of AQP2
Ramachandran et al., Genetics 2011 : Using a number of reporters for the PKA pathway, we found that the inhibition of TORC1 did not result in diminished PKA signaling activity
McArdle et al., J Virol 2011 : We find that inhibition of calmodulin dependent kinase kinase ( CaMKK ), but not calmodulin dependent kinase II ( CaMKII ) or protein kinase A (PKA) , blocks HCMV mediated activation of glycolysis
Murshid et al., PloS one 2010 : These findings of PKA regulation of HSF1 through S320 phosphorylation add to our knowledge of the signaling networks converging on this factor and may contribute to elucidating its complex roles in the stress response and understanding HSF1 dysregulation in disease
Shen et al., Cell cycle (Georgetown, Tex.) 2010 (Infertility, Female) : Our results suggest that PKA induced inhibition of Plk1 may be critical in oocyte meiotic arrest and female infertility in PDE3A ( -/- ) mice
Li et al., Nutr Cancer 2011 (Breast Neoplasms) : Adiponectin also increased intracellular levels of cAMP and the activity of protein kinase-A (PKA)
Liu et al., Biochem Biophys Res Commun 2011 : On the sarcoplasmic reticulum ( SR ) in cardiomyocytes, PKA regulates the calcium cycling for exciting-contraction coupling, which is often dysfunctional in a variety of heart diseases including heart failure
Cohen et al., BMC cancer 2010 (Cerebellar Neoplasms...) : PACAP and pharmacological activation of PKA also inhibited proliferation
Jefcoate et al., Mol Cell Endocrinol 2011 : StAR transcription is directly activated by PKA phosphorylation
Maldonado et al., Cancer Res 2010 (Carcinoma, Hepatocellular...) : Protein kinase A (PKA) activation by cAMP analogues and glycogen synthase kinase 3ß ( GSK-3ß ) inhibition decreased d?, whereas PKA inhibition hyperpolarized, consistent with reports that PKA and GSK-3ß decrease and increase VDAC conductance, respectively
Lee et al., J Biol Chem 2011 : We previously reported that dehydroepiandrosterone stimulated phosphorylation of FoxO1 in endothelial cells requires cAMP dependent protein kinase a (PKA-a)
Christian et al., J Biol Chem 2011 (Chronic Disease...) : Small molecule AKAP-protein kinase A (PKA) interaction disruptors that activate PKA interfere with compartmentalized cAMP signaling in cardiac myocytes
Breitbart et al., Asian J Androl 2011 : In the capacitation process, the EGFR is partially activated by protein kinase A (PKA) , resulting in phospholipase D ( PLD ) activation and actin polymerization ... Under in vivo conditions, the EGFR can be directly activated by its known ligand epidermal growth factor (EGF), and indirectly by activating PKA or by transactivation mediated by G protein coupled receptors ( GPCRs ) activation or by ouabain
Pulinilkunnil et al., J Biol Chem 2011 : Both PKA and AKT inhibitors attenuated AMPK Ser ( 485/491 ) phosphorylation resulting from a-adrenergic blockade and prevented decreases in AMPK activity
Zeng et al., PloS one 2010 : We further show that activation of protein kinase A (PKA) efficiently inhibits ciliary localization of Gli2 and Gli3, but only moderately affects the ciliary localization of Gli1 ... We further show that activation of protein kinase A (PKA) efficiently inhibits ciliary localization of Gli2 and Gli3 , but only moderately affects the ciliary localization of Gli1 ... We further show that activation of protein kinase A (PKA) efficiently inhibits ciliary localization of Gli2 and Gli3, but only moderately affects the ciliary localization of Gli1
Líbano-Soares et al., Arch Biochem Biophys 2011 : The inhibitory effect of PGE2 on PT Na+-ATPase activity involves G-protein and the activation of protein kinase A (PKA) because : ( 1 ) PGE2 increased [ 35S ] GTP?S binding ; ( 2 ) GDPßS abolished the inhibitory effect of PGE2 ; ( 3 ) PGE2 increased PKA activity ; ( 4 ) the inhibitory effect of PGE2 was abolished by PKA inhibitor peptide ... We observed that the PKA mediated inhibitory effect of PGE2 on PT Na+-ATPase activity requires previous activation of protein kinase C
Kim et al., J Biol Chem 2011 : AKAP10 also mediated PGE ( 2 ) potentiation of the expression of cytokines IL-10 and IL-6, whereas PGE ( 2 ) suppression of TNF-a was mediated by AKAP8 anchored PKA-RII
Li et al., J Neurosci 2011 (Pain) : Mitochondrial reactive oxygen species are activated by mGluR5 through IP3 and activate ERK and PKA to increase excitability of amygdala neurons and pain behavior
Chen et al., J Biol Chem 2011 (Colonic Neoplasms) : The interaction is dependent on protein kinase A (PKA) mediated phosphorylation of hDpr1 at Ser-237 and Ser-827 ... Together, our findings establish a novel layer of regulation of Wnt signaling by PKA via the 14-3-3-Dpr1-Dvl axis
Gobejishvili et al., J Pharmacol Exp Ther 2011 : The SAM mediated decrease in LPS-inducible Pde4b2 up-regulation resulted in an increase in cellular cAMP levels and activation of cAMP dependent protein kinase A (PKA) , which plays an inhibitory role in LPS induced TNF production ... These results demonstrate for the first time that inhibition of LPS induced PDE4B2 up-regulation and increased cAMP dependent PKA activation are significant mechanisms contributing to the anti-TNF effect of SAM
Cho et al., Biochem J 2011 : Interestingly, dual inhibition of PKA and Ras completely inhibited PLD1 expression and cAMP induced decidualization ... These results suggest that PLD1 expression during decidualization is controlled additively by PKA and Ras
Wanichawan et al., Am J Physiol Cell Physiol 2011 : These data indicate that the novel in vitro PKA phosphorylation site is inaccessible in full-length as well as in calpain- or caspase-3 digested NCX1 protein, suggesting that NCX1 is not a direct target for PKA phosphorylation
Bonfleur et al., Metabolism 2011 : Insulin secretion induced by the protein kinase A (PKA) activators forskolin and 3-isobutyl-1-methyl-xanthine, in the presence of 11.1 mmol/L glucose, was lower in LDLR ( -/- ) islets and was normalized in the presence of the protein kinase C pathway activators carbachol and phorbol 12-myristate 13-acetate
Smith et al., Cell cycle (Georgetown, Tex.) 2011 : AKAP-Lbc also directs PKA phosphorylation of KSR-1 on a critical residue to ensure maximal signaling efficiency
Saraiva et al., PloS one 2011 (Malaria, Falciparum) : These results indicate that the effect of Ang- ( 1-7 ) on P. falciparum blood stage involves a MAS mediated PKA inhibition
Wan et al., Science signaling 2011 (Second Messenger Systems) : Knockdown of LRP6 disrupted the localization of Ga ( s ) to the plasma membrane, which led to a decrease in the extent of coupling of Ga ( s ) to PTH1R and inhibited the production of cAMP and the activation of cAMP dependent protein kinase (PKA) in response to PTH
Ishiguro et al., Curr Neurovasc Res 2011 (Hypoxia-Ischemia, Brain) : P13-K inhibition by LY294002 had no influence on the effects of cilostazol, but inhibition of cAMP/PKA with PKA inhibitor and Rp-cAMPS suppressed cilostazol induced inhibition of cell damage and promotion of VE-cadherin expression
Goto et al., Mol Biol Cell 2011 : dbcAMP induced Rac1 activation was principally mediated by protein kinase A (PKA) and Sif- and Tiam1-like exchange factor ( STEF ) /Tiam2
Li et al., Mol Med Report 2011 (Adenocarcinoma...) : Fibronectin increases RhoA activity through inhibition of PKA in the human gastric cancer cell line SGC-7901 ... Results from laboratories, including ours, have demonstrated that PKA inhibits the activity and function of RhoA
Perino et al., Mol Cell 2011 (Heart Failure...) : Anchored PKA activates PDE3B to enhance cAMP degradation and phosphorylates p110? to inhibit PIP ( 3 ) production
Matsumoto et al., Mol Med Report 2008 : In K1 cells, B-Raf might predominate over Raf-1, and the elevated cAMP is thought to promote MAPK phosphorylation through the PKA mediated activation of Rap1
McDougall et al., Synapse 2011 : PKA activity of Mn-treated rats was enhanced in both the dorsal striatum and PFC, whereas p-Akt levels were elevated in the dorsal striatum
Cousin et al., J Physiol 2011 (Calcium Signaling...) : Finally, we found that stimulation of synapsin phosphorylation, but not RIM1a, by PKA was frequency dependent and long lasting ... Finally, we found that stimulation of synapsin phosphorylation, but not RIM1a , by PKA was frequency dependent and long lasting
Cui et al., Learn Mem 2011 : GluA1 PKA dependent phosphorylation peaked 10 min after the 10-min training trial and returned to baseline within 90 min ... PKA mediated GluA1 phosphorylation and later GluA1 insertion could, conjointly, provide increased AMPA function to support both short-term and long-term appetitive memory
Pernalete et al., Endocrinology 1990 : Recent studies indicate that PTH binding promotes not only stimulation of adenylate cyclase and activation of protein kinase-A (PK-A) , but also, stimulation of phospholipase-C, leading to activation of PK-C
Sroubek et al., J Biol Chem 2011 : A PKA inhibitor targeted to the ER surface ( termed p4PKIg ) completely abolished PKA mediated augmentation of HERG in HEK293 cells as well as rat neonatal cardiomyocytes ... Our results support a model where PKA dependent regulation of HERG synthesis occurs at the ER surface
Makarova et al., J Biol Chem 2011 (Acute Lung Injury...) : uPA induced phosphorylation of eNOS was also inhibited by the protein kinase A (PKA) inhibitor, myristoylated PKI, but was not dependent on PI3K-Akt signaling ... uPA induced phosphorylation of eNOS was also inhibited by the protein kinase A (PKA) inhibitor, myristoylated PKI, but was not dependent on PI3K-Akt signaling ... uPA induced phosphorylation of eNOS was also inhibited by the protein kinase A (PKA) inhibitor, myristoylated PKI, but was not dependent on PI3K-Akt signaling ... LRP blockade and inhibition of PKA prevented uPA- and uPA-PAI-1 induced permeability of PMVEC monolayers in vitro and uPA induced lung permeability in vivo
Shiryaev et al., Journal of molecular signaling 2011 : The MAPKAPKs MK2, MK3 and MK5 are closely related, but MK2 and MK3 are the major downstream targets of the p38MAPK pathway, while MK5 can be activated by the atypical MAPK ERK3 and ERK4, protein kinase A (PKA) , and maybe p38MAPK ... Therefore, we wanted to investigate the implication of MK2 and MK5 in stress- and PKA induced HSP27 phosphorylation
Ghosh et al., Nature 1990 (Second Messenger Systems) : Using gel retardation assays we found that PKC, cyclic AMP dependent protein kinase (PKA) and a haem regulated eIF-2 kinase ( HRI ) could activate NF-kappa B in vitro, whereas casein kinase II was ineffective
Yen et al., J Biomed Sci 2011 : The possibility of PKA dependent nNOS activation was substantiated by immunohistochemical demonstration of co-localization of PKA and nNOS in putative NTS neurons
Mahata et al., Cell Tissue Res 2011 : In agreement, secretin increased PKA activity and induced phosphorylation of CREB and binding to Th CRE, suggesting secretin signaling to transcription via a PKA-CREB pathway
Baxter et al., J Neurochem 2011 : Here, we show that in cortical neurons, PACAP induced PKA signaling exerts a major part of its neuroprotective effects indirectly, by triggering action potential ( AP ) firing
Begum et al., J Biol Chem 2011 (MAP Kinase Signaling System) : We conclude that PDE3A regulates VSMC growth via two complementary pathways, i.e. PKA catalyzed inhibitory phosphorylation of Raf-1 with resulting inhibition of MAPK signaling and PKA/CREB mediated induction of p21 , leading to G0/G1 cell cycle arrest, as well as by increased accumulation of p53, which induces MKP-1, p21, and WIP1, leading to inhibition of G1 to S cell cycle progression ... We conclude that PDE3A regulates VSMC growth via two complementary pathways, i.e. PKA catalyzed inhibitory phosphorylation of Raf-1 with resulting inhibition of MAPK signaling and PKA/CREB mediated induction of p21, leading to G0/G1 cell cycle arrest, as well as by increased accumulation of p53, which induces MKP-1, p21, and WIP1, leading to inhibition of G1 to S cell cycle progression
Kim et al., Arch Pharm Res 2011 (Parkinsonian Disorders...) : Interference of alpha-synuclein with cAMP/PKA dependent CREB signaling for tyrosine hydroxylase gene expression in SK-N-BE ( 2 ) C cells
Bradshaw et al., J Neurosci 2011 : PKA phosphorylation of NDE1 is DISC1/PDE4 dependent and modulates its interaction with LIS1 and NDEL1
Sucharov et al., J Card Fail 2011 : Acute ß ( 1 ) -AR stimulation or expression of constitutively active ( CA ) PKA reduced a ( 1 ) -adrenergic mediated phosphorylation of HDAC5 and PKD by activation of a phosphatase ... Acute ß ( 1 ) -AR stimulation or expression of constitutively active ( CA ) PKA reduced a ( 1 ) -adrenergic mediated phosphorylation of HDAC5 and PKD by activation of a phosphatase
Su et al., Science signaling 2011 : Phosphorylation of Smo was initiated by adenosine 3',5'-monophosphate ( cAMP ) -dependent protein kinase (PKA) and further enhanced by casein kinase I ( CKI )
Macdougall et al., J Mol Cell Cardiol 2012 : This was not linked to modulation of L-type Ca ( 2+ ) current, but instead to a discrete PKA mediated phosphorylation of phospholamban at Ser ( 16 )
Jian et al., Steroids 2011 (Melanoma, Experimental) : Diethylstilbestrol enhances melanogenesis via cAMP-PKA mediating up-regulation of tyrosinase and MITF in mouse B16 melanoma cells ... Diethylstilbestrol enhances melanogenesis via cAMP-PKA mediating up-regulation of tyrosinase and MITF in mouse B16 melanoma cells
Xie et al., Cell Signal 2011 : In this study, we show that the pharmacological elevation of [ cAMP ] ( i ) in mouse embryonic fibroblasts ( MEFs ) and human embryonic kidney 293 (HEK293) cells inhibits mTORC1 activation via a PKA dependent mechanism
Persaud et al., Biochem Biophys Res Commun 1990 : The involvement of cyclic AMP dependent protein kinase A (PKA) in the exocytotic release of insulin from rat pancreatic islets was investigated using the Rp isomer of adenosine 3',5'-cyclic phosphorothioate ( Rp-cAMPS ) ... These results suggest that cAMP induced activation of PKA is not essential for either basal or glucose stimulated insulin secretion from rat islets
Moncada et al., Proc Natl Acad Sci U S A 2011 : Moreover, activation of the glutamatergic NMDA receptors is required for setting the learning tags, and this machinery further required a-Ca ( 2+ ) /calmodulin dependent protein kinase II and PKA but not ERK1/2 activity
Moon et al., Exp Cell Res 2011 : PKA inhibitor, H-89, reduced CREB ( S133 ) phosphorylation and mBAFF expression in control and LPS stimulated macrophages
Deb et al., Mol Cell Biochem 2011 : The results indicate that downregulation of testicular CYP1B1 by estradiol was independent of PKA activity and was not mediated by ERs in MA-10 cells
Nishioka et al., Arterioscler Thromb Vasc Biol 2011 (Calcium Signaling) : PKA mediated phosphorylation of TRPC6 at Thr69 is essential for the vasorelaxant effects of PDE3 inhibition against the vasoconstrictive actions of Ang II
Wu et al., Mol Pharmacol 2011 : Moreover, PKA inhibition prevented the activation of LKB1 , supporting the fact that PKA regulates LKB1 ... In conclusion, rimonabant has an antilipogenic effect in hepatocytes by inhibiting LXRa dependent SREBP-1c induction, as mediated by an increase in PKA activity and PKA mediated LKB1 activation downstream of CB1 coupled Ga ( i/o ) inhibition
Gangopahyay et al., J Biol Chem 2011 : The stimulation of eNOS activity by BMPRII ligands was largely dependent on protein kinase A (PKA) activation, as demonstrated using the PKA inhibitors H89 and myristoylated PKI ( 6-22 ) amide
Reyes et al., Mol Membr Biol 2011 : Our data support a role for PKA and PKC in the phosphorylation of ENT1 within the intracellular loop and show that PKA can phosphorylate multiple sites within this loop while PKC specifically targets serines 279 and 286 and threonine 274
Shelly et al., Neuron 2011 : Fluorescence resonance energy transfer ( FRET ) imaging showed that Sema3A elevated the cGMP but reduced cAMP and protein kinase A (PKA) activity, and its axon suppression is attributed to the downregulation of PKA dependent phosphorylation of axon determinants LKB1 and GSK-3ß
Liu et al., J Mol Cell Cardiol 2011 (Calcium Signaling) : Inhibition of intrinsic PKA activity or inhibition of PDE in SANC, respectively : reduces or increases PLB phosphorylation, and markedly prolongs or reduces the LCR period ; and markedly reduces or accelerates SAN spontaneous firing rate
Torres-Altoro et al., J Neurochem 2011 (Neurotoxicity Syndromes...) : CPF potentiated protein kinase A (PKA) dependent phosphorylation of the striatal protein dopamine- and cAMP regulated phosphoprotein of M ( r ) 32 kDa ( DARPP-32 ) and the glutamate receptor 1 (GluR1) subunit of a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid ( AMPA ) receptors in mouse brain slices
Tulloch et al., J Biol Chem 2011 : Phosphorylation of PLM at serine 68 by PKA in ARVM or transiently transfected HEK cells increased its palmitoylation, but PKA activation did not increase the palmitoylation of S68A PLM-YFP in HEK cells
Liu et al., BMB Rep 2011 : FSCB phosphorylation could be induced by PKA agonist DB-cAMP, but was blocked by PKA antagonist H-89.Therefore, FSCB contributes to spermatozoa capacitation in a tyrosine phosphorylated format, which may help in further elucidating the molecular mechanism of spermatozoa capacitation
Wierstra et al., Biochem Biophys Res Commun 2011 : The transcription factor FOXM1c is activated by protein kinase CK2, protein kinase A (PKA) , c-Src and Raf-1 ... Indeed, full-length FOXM1c is strongly activated by protein kinase CK2 and PKA ( protein kinase A ) ... Both CK2 and PKA do not activate a FOXM1c deletion mutant without N-terminus demonstrating that the activation of FOXM1c by CK2 and PKA depends on the presence of the N-terminus ... Consequently, CK2 and PKA activate FOXM1c by alleviating the inhibition of FOXM1c by its N-terminus ... The presence of two potential CK2 phosphorylation sites and two potential PKA phosphorylation sites in the N-terminus of FOXM1c suggests that CK2 and PKA may activate FOXM1c through phosphorylation of the FOXM1c N-terminus ... Thus, CK2 and PKA strongly activate full-length FOXM1c because they alleviate the repression of FOXM1c by its own auto-inhibitory N-terminus
Henri et al., J Cell Physiol 2012 : We then assessed ROS production immediately after UVA exposure and found that : ( 1 ) UVA-ROS production was strongly reduced in HaCaT-MC1R but not in HaCaT-R ( 151 ) C cells compared to parental HaCaT cells ; ( 2 ) this inhibitory effect was further amplified by incubation of HaCaT-MC1R cells with a-MSH before UVA exposure ; ( 3 ) protein kinase A (PKA) dependent NoxA1 phosphorylation was increased in HaCaT-MC1R compared to HaCaT and HaCaT-R ( 151 ) C cells
Brown et al., Mol Cell Neurosci 2012 (Neurofibromatosis 1) : Third, neurofibromin regulates CNS neurite length and growth cone areas in a cAMP/PKA/Rho/ROCK dependent manner in vitro and in vivo
Mao et al., Proc Natl Acad Sci U S A 2011 (Obesity) : Glucagon stimulated protein kinase PKA , which in turn phosphorylated IRE1a at Ser ( 724 ), a highly conserved site within the kinase activation domain
Yger et al., Frontiers in behavioral neuroscience 2011 : It is one of three related, PKA regulated inhibitors of protein phosphatase-1 (PP1)
Benninger et al., J Physiol 2011 : Forced elevation of cAMP led to PKA mediated increases in insulin secretion from islets lacking gap-junctions, but not from islets expressing Cx36 gap junctions
Yen et al., J Biol Chem 2011 (MAP Kinase Signaling System) : We show that PGE2 induced MMP-9 expression is mediated primarily through the EP2/EP4  cAMP  protein kinase A (PKA)/PI3K  ERK signaling pathway, leading to c-Fos expression, and through JNK mediated activation of c-Jun in a PKA/PI3K/ERK independent manner ... PKA , PI3K, and ERK inhibitors abolished PGE2- and cAMP induced c-Fos and MMP-9 up-regulation, and ERK activation was required for the binding of activator protein 1 (AP-1) transcription factor to the MMP-9 promoter ... PKA , PI3K, and ERK inhibitors abolished PGE2- and cAMP induced c-Fos and MMP-9 up-regulation, and ERK activation was required for the binding of activator protein 1 (AP-1) transcription factor to the MMP-9 promoter ... PKA , PI3K, and ERK inhibitors abolished PGE2- and cAMP induced c-Fos and MMP-9 up-regulation, and ERK activation was required for the binding of activator protein 1 (AP-1) transcription factor to the MMP-9 promoter
Van de Velde et al., Proc Natl Acad Sci U S A 2011 : Increases in PKA activity stimulate the phosphorylation of CREB , which in turn enhances beta cell survival by upregulating IRS2 expression
Pongratz et al., Ann Rheum Dis 2012 (Arthritis, Experimental) : Interleukin 10 (IL-10) producing B cells suppress arthritis and are a potential target of the SNS because ( 1 ) B cells express functional ß ( 2 ) -adrenoceptors ( ß ( 2 ) ARs ) and ( 2 ) IL-10 , at least in monocytes/macrophages, is regulated in a cAMP/PKA/CREB dependent manner
Husse et al., Heart international 2010 : The inhibition of PKA , PKC, MEK, p38-MAPK or PI3-kinase partially reduced the strain induced CREB phosphorylation ... Activation of PKA by forskolin or PKC by PMA resulted in a level of CREB phosphorylation comparable to the reduced level of the strain induced CREB phosphorylation in the presence of PKA or PKC inhibitors ... The strain induced c-Fos expression can be reduced by PKC inhibition but not by PKA inhibition
Lv et al., Leukemia 2012 (Precursor T-Cell Lymphoblastic Leukemia-Lymphoma) : High levels of miR-142-3p resulted in low levels of cAMP and weak activity of PKA , thus relieving the inhibitory effect of PKA on T-leukemic cell proliferation
Spirli et al., Hepatology 2012 (Calcium Signaling) : In Pkd2KO cells, ER-Ca ( 2+ ) depletion increases cAMP and PKA dependent ERK1/2 activation and both are inhibited by STIM1 inhibitors or by silencing of adenylyl cyclase type 6 ( AC6 )
Inoue et al., Am J Physiol Regul Integr Comp Physiol 2012 (Disease Models, Animal...) : In addition, the baseline PKA dependent NHE3 phosphorylation at serine 552 was reduced in renal cortical membranes of rats with HF. Collectively, these results suggest that NHE3 is upregulated in the proximal tubule of HF rats by transcriptional, translational, and posttranslational mechanisms
Panas et al., J Neuroimmune Pharmacol 2012 : Furthermore, we observed a TAAR1 dependent phosphorylation of PKA and PKC following treatment with methamphetamine in transfected HEK293 cells, immortalized rhesus monkey B cells and PHA activated rhesus monkey lymphocytes
Chin et al., Am J Physiol Gastrointest Liver Physiol 2012 (Crohn Disease...) : Secretion was also inhibited by H-89 ( PKA inhibitor ) while Tph1 and VMAT1 transcription was regulated by PKA/MAPK and PI3K mediated signaling ... Secretion was also inhibited by H-89 ( PKA inhibitor ) while Tph1 and VMAT1 transcription was regulated by PKA/MAPK and PI3K mediated signaling
Chen et al., Proc Natl Acad Sci U S A 2011 (Synaptic Transmission) : The tomosyn-SNARE interaction is regulated by protein kinase A (PKA) , an enzyme implicated in learning and memory, suggesting tomosyn could be an important effector in PKA dependent synaptic plasticity
O'Connor et al., Am J Physiol Cell Physiol 2012 : Finally, we find that the regulation of Rac1 and RhoA in response to LPA is differentially regulated by phosphodiesterases, PKA , and phosphatidylinositol 3-kinase, thus supporting their spatially distinct compartmentalization ... Finally, we find that the regulation of Rac1 and RhoA in response to LPA is differentially regulated by phosphodiesterases, PKA , and phosphatidylinositol 3-kinase, thus supporting their spatially distinct compartmentalization
Dickey et al., J Neurosci 2011 : PKA/AKAP1 mediated phosphorylation of Drp1 at Ser656 increased mitochondrial length and dendrite occupancy, enhancing dendritic outgrowth but paradoxically decreasing synapse number and density
Enomoto et al., Free Radic Biol Med 2012 : GSK-3ß phosphorylated STK38 on residues S6 and T7 in vitro, depending largely on a PKA mediated priming phosphorylation of STK38 on residues S10 and S11, respectively
Guo et al., Chin Med J (Engl) 2010 (Fibrosis...) : The effects of the protein kinase A (PKA) activator 8-Br-cAMP and protein kinase C ( PKC ) activator phorbol 12-myristate 13-acetate ( PMA ) on MAPK phosphorylation, and the effects of the PKA inhibitor H89 and PKC inhibitor calphostin C on MAPK phosphorylation and CTGF expression were detected by immunoblotting assay ... A PKA activator as well as PKC activators induced MAPK phosphorylation, and both PKA and PKC inhibitors antagonized PTH induced MAPK phosphorylation and CTGF expression ... The effects of the protein kinase A (PKA) activator 8-Br-cAMP and protein kinase C ( PKC ) activator phorbol 12-myristate 13-acetate ( PMA ) on MAPK phosphorylation, and the effects of the PKA inhibitor H89 and PKC inhibitor calphostin C on MAPK phosphorylation and CTGF expression were detected by immunoblotting assay
Chang et al., J Cell Biochem 2012 (MAP Kinase Signaling System) : Collectively, quercetin induced EGR-1 expression is largely dependent on PKA and partly on p38 MAPK pathway, and SRE sites of EGR-1 promoter are involved in quercetin induced EGR-1 transcriptional activity
Ji et al., Br J Pharmacol 2012 : Signalling pathway inhibitors were used to examine the roles of Rho, PKA , the cAMP response element binding protein ( CREB ) and NF-?B on the PGE ( 2 ) stimulated up-regulation of Nurr1 ... PGE ( 2 ) stimulation of the EP1 receptor induced the phosphorylation and activation of CREB and NF-?B, which could be blocked by inhibition of PKA
Úriz et al., PloS one 2011 (Choledochal Cyst...) : Experiments with inhibitors and Ca ( 2+ ) -chelator confirmed that the synergistic effect of secretin plus TUDCA involves microtubules, intracellular Ca ( 2+ ), PKCa, PI3K, PKA and MEK pathways ... UDCA is conjugated in order to promote secretin stimulated hydrocholeresis in rats through Ae2, microtubules, intracellular Ca ( 2+ ), PKCa, PI3K, PKA , and MEK
Goncharova et al., Am J Respir Cell Mol Biol 2012 : Vasodilator stimulated phosphoprotein ( VASP ), an anticapping protein, modulates the formation of actin stress fibers during cell motility, and is negatively regulated by protein kinase A (PKA)-specific inhibitory phosphorylation at serine 157 ( Ser157 ) ... Here, we show that treatment with ß ( 2 ) AR agonists and PGE2 induces the PKA dependent phosphorylation of VASP and inhibits the migration of HASM cells
Lee et al., Biochim Biophys Acta 2012 : Regulation of SGLT expression and localization through Epac/PKA dependent caveolin-1 and F-actin activation in renal proximal tubule cells ... In addition, 8-Br-cAMP induced PKA and Epac activation increased phosphorylation of extracellular signal regulated kinase ( ERK ), p38 mitogen activated protein kinase ( MAPK ), and nuclear factor kappa B ( NF-?B ), which were involved in expressions of SGLTs
Czuriga et al., Basic Res Cardiol 2012 (Disease Models, Animal...) : The level of protein kinase A (PKA) dependent cardiac troponin I (TnI) phosphorylation was lower in the myocardium surrounding the pacing electrode ( pacing site ) of the failing left ventricle ( LV ) than in the controls
Gallegos et al., Dev Biol 2012 (Wnt Signaling Pathway) : PKA induction of canonical Wnt signaling during tubulogenesis was confirmed genetically using MM from Batgal-reporter mice
Ji et al., Liver Transpl 2012 (Disease Models, Animal...) : In vitro, cAMP-PKA activation diminished macrophage tumor necrosis factor a, IL-6, and IL-12 in an IL-10 dependent manner and prevented necrosis/apoptosis in primary mouse hepatocyte cultures ... In vitro, cAMP-PKA activation diminished macrophage tumor necrosis factor a, IL-6 , and IL-12 in an IL-10 dependent manner and prevented necrosis/apoptosis in primary mouse hepatocyte cultures
Deyts et al., J Neurosci 2012 : We found that accumulation of APP C-terminal fragments or expression of membrane tethered APP intracellular domain results in adenylate cyclase dependent activation of PKA ( protein kinase A ) and inhibition of GSK3ß signaling cascades, and enhancement of axodendritic arborization in rat immortalized hippocampal neurons, mouse primary cortical neurons, and mouse neuroblastoma
Monterisi et al., J Cell Sci 2012 : NHERF1 overexpression in CFBE41o-cells restores chloride secretion, subcortical cAMP compartmentalization and local PKA activity, indicating that regulation of dF508CFTR function requires not only stable expression of the mutant CFTR at the cell surface but also depends on both generation of local cAMP signals of adequate amplitude and activation of PKA in proximity of its target ... Our data show that a sufficiently high concentration of cAMP in the subcortical compartment is required to achieve PKA mediated regulation of CFTR activity
Li et al., Oncogene 2012 (Precursor T-Cell Lymphoblastic Leukemia-Lymphoma) : Thus, our data revealed a novel interplay between PKA phosphorylation and TAL1 mediated epigenetic regulation that regulates hematopoietic transcription and differentiation programs during hematopoiesis and leukemogenesis
Murai et al., J Neurophysiol 2012 : The Ca ( 2+ ) /calmodulin stimulated adenylate cyclase may contribute to the activation of PKA
Liu et al., Biochimie 2012 : Adiponectin levels are also stimulated by FOXO1 and AMP activated protein kinase (AMPK), and are suppressed by PKA or silencing mediator of retinoid and thyroid hormone receptors ( SMRT ) ... Adiponectin levels are also stimulated by FOXO1 and AMP activated protein kinase (AMPK), and are suppressed by PKA or silencing mediator of retinoid and thyroid hormone receptors ( SMRT ) ... Adiponectin levels are also stimulated by FOXO1 and AMP activated protein kinase (AMPK) , and are suppressed by PKA or silencing mediator of retinoid and thyroid hormone receptors ( SMRT )
Damm et al., Mol Endocrinol 2012 (MAP Kinase Signaling System) : Melanocortin induced PKA activation inhibits AMPK activity via ERK-1/2 and LKB-1 in hypothalamic GT1-7 cells ... Inhibitory effects of a-MSH on AMPK were blocked by specific inhibitors of protein kinase A (PKA) or ERK-1/2, pointing to an important role of both kinases in this process
Oldenburger et al., PloS one 2012 (Pulmonary Disease, Chronic Obstructive) : CSE decreased Epac1 expression, but did not affect Epac2 and PKA expression
Lee et al., J Biol Chem 2012 : These results suggest that PKA dependent phosphorylation and microtubule binding of IP(3)K-A are involved in its regulatory mechanism for activity dependent neuronal events such as local calcium signaling and its synaptic targeting
Aksaas et al., Genes & cancer 2011 : We conclude that PKA phosphorylation of SRSF1 at serine 119 regulates SFRS1 dependent RNA binding and processing but not its interaction with PKA
Michel et al., J Neurosci 2012 : Using Western blotting, we found that sustained MAPK phosphorylation was dependent upon protein synthesis, but not PKA or PKC activity
Frasch et al., Prog Lipid Res 2012 (Inflammation) : In macrophages, this early acting pathway results in PKA dependent augmentation of Rac1 activity via increased production of PGE2 and cAMP
Somvanshi et al., Int J Cardiol 2013 (Cardiomegaly) : SST enhances ß2AR agonist formoterol mediated effects on PKA , CREB and ERK1/2 phosphorylations whereas it inhibits isoproterenol mediated ERK1/2 and p38 signaling in concentration dependent manner ... SST enhances ß2AR agonist formoterol mediated effects on PKA , CREB and ERK1/2 phosphorylations whereas it inhibits isoproterenol mediated ERK1/2 and p38 signaling in concentration dependent manner
Ghorpade et al., Mol Cell Biol 2012 : Genetic and signaling perturbations presented the evidence that miR-155 regulates PKA signaling by directly targeting a negative regulator of PKA, protein kinase inhibitor alpha ( PKI-a ) ... Enhanced activation of PKA signaling resulted in the generation of PKA C-a ; phosphorylation of MSK1, cyclic AMP response element binding protein ( CREB ), and histone H3 ; and recruitment of phospho-CREB to the apoptotic gene promoters ... Enhanced activation of PKA signaling resulted in the generation of PKA C-a ; phosphorylation of MSK1 , cyclic AMP response element binding protein ( CREB ), and histone H3 ; and recruitment of phospho-CREB to the apoptotic gene promoters
Zimmerman et al., Cell death & disease 2012 (Neoplasms) : Apoptin interacts with the PKA associating protein BCA3/AKIP1 , and inhibition of PKA in tumor cells by treatment with H89 increased the phosphorylation of apoptin, whereas the PKA activator cAMP partially reduced it
Ullrich et al., J Mol Cell Cardiol 2012 : During physical exercise and stress, the sympathetic system stimulates cardiac contractility via ß-adrenergic receptor activation, resulting in protein kinase A (PKA) mediated phosphorylation of the cardiac ryanodine receptor , RyR2, at Ser2808 ... We show here by three independent experimental approaches that PKA dependent RyR2-S2808 phosphorylation plays significant functional roles at the subcellular level, namely, Ca ( 2+ ) release synchronization, Ca ( 2+ ) wave propagation and functional adaptation of RyR2 to variable [ Ca ( 2+ ) ] ( SR )
Wang et al., Nature 2012 (Calcium Signaling...) : Glucagon promotes CRTC2 dephosphorylation in part through the protein kinase A (PKA) mediated inhibition of the CRTC2 kinase SIK2
Koike et al., J Biol Chem 2012 : In summary, our data suggest that Rad24 and Rad25 negatively regulate Prz1 and that Pka1 , Msn5, Pac1, Tfs1, and Ape2 also regulate Prz1
Jones et al., Cell Signal 2012 : Consistent with these observations, cAMP elevation triggered the PKA dependent phosphorylation of RhoA on serine 188, and a non-phosphorylatable Ser188Ala RhoA mutant functioned as a dominant negative inhibitor of cAMP mediated neuroendocrine phenotype formation ... These results suggest that PKA mediated inhibition of RhoA via its phosphorylation on serine 188 and the subsequent inhibition of ROCK activity plays a key role in determining initial changes in cellular morphology during LNCaP cell differentiation to a neuroendocrine phenotype ... These results suggest that PKA mediated inhibition of RhoA via its phosphorylation on serine 188 and the subsequent inhibition of ROCK activity plays a key role in determining initial changes in cellular morphology during LNCaP cell differentiation to a neuroendocrine phenotype
Dell'anno et al., Neuropharmacology 2013 : The ability of DHPG to increase PKA dependent phosphorylation of GluA1 depends on concomitant activation of the dopamine- and cAMP regulated phosphoprotein of 32kDa ( DARPP-32 )
Bakouh et al., PloS one 2012 : When NPT2a-oocytes were injected with NEG2, a short amino-acid sequence from the CFTR regulatory domain that regulates PKA dependent CFTR trafficking to the plasma membrane, IPi values and NPT2a membrane expression were diminished, and could be enhanced by PKA stimulation, thereby mimicking the effects of CFTR co-expression
Ji et al., Hepatology 2013 (Liver Diseases...) : In vitro, PACAP treatment not only diminished macrophage tumor necrosis factor alpha/IL-6/IL-12 levels in a PKA dependent manner, but also prevented necrosis and apoptosis in primary mouse hepatocyte cultures ... In vitro, PACAP treatment not only diminished macrophage tumor necrosis factor alpha/IL-6/IL-12 levels in a PKA dependent manner, but also prevented necrosis and apoptosis in primary mouse hepatocyte cultures ... In vitro, PACAP treatment not only diminished macrophage tumor necrosis factor alpha/IL-6/IL-12 levels in a PKA dependent manner, but also prevented necrosis and apoptosis in primary mouse hepatocyte cultures ... Conclusion : Our novel findings document the importance of PACAP mediated cAMP-PKA signaling in hepatic homeostasis and cytoprotection in vivo
Huang et al., Pain 2012 (Hyperalgesia...) : Here, we report that protease activated receptor 2 (PAR2) activation after CCD or dissociation mediates the increase of cAMP activity and protein kinase A (PKA) and cAMP dependent hyperexcitability and hyperalgesia in rats
Lin et al., J Biomed Sci 2012 : We further showed that the capt/ssh mutant phenotypes depended on the inactivation of protein kinase A (PKA) and activation of Rho
Kumari et al., Molecular vision 2012 : Effects of phosphokinase A (PKA) agonist cyclic AMP ( cAMP ), and antagonist H-89 on AQP5 expression and localization were studied in vitro using MDCK ( Madin-Darby Canine Kidney ) cells, and ex vivo using isolated corneas from wild type mice ... This is the first report on the spatial expression of AQP5 in the corneal keratocytes and lens epithelial cells, as well as on the regulation of AQP5 localization by PKA in the corneal epithelial cells ... PKA mediated regulation of AQP5 holds promise for therapeutic intervention to control corneal and lens diseases
Nin et al., J Biol Chem 2012 : We observed that PKA activation leads to a fast and transient activation of SIRT1 that is DBC1 dependent ... In fact, an increase in cAMP/PKA activity resulted in the dissociation of SIRT1 and DBC1 in an AMP activated protein kinase (AMPK) dependent manner ... We propose that PKA and AMPK can acutely activate SIRT1 by inducing dissociation of SIRT1 from its endogenous inhibitor DBC1
Hendarto et al., Metabolism 2012 (Albuminuria...) : This effect was reversed by both PKA inhibitor H89 and adenylate cyclase inhibitor SQ22536, but not by Epac2 inhibition via its small interfering RNA ... Liraglutide may have a direct beneficial effect on oxidative stress and diabetic nephropathy via a PKA mediated inhibition of renal NAD(P)H oxidase , independently of a glucose lowering effect ... Liraglutide may have a direct beneficial effect on oxidative stress and diabetic nephropathy via a PKA mediated inhibition of renal NAD(P)H oxidase , independently of a glucose lowering effect
Savchenko et al., Neurotox Res 2013 : However, it is unknown whether PKA regulates NADPH oxidase gene expression in neurons and microglia ... The activation of PKA with agonist dibutyryl cAMP ( dbcAMP ) ( 100 µM ) induced the upregulation of Arg1 gene expression in microglia involving in the process of microglial activation ... The activation of PKA for 24 h induced the transcriptional upregulation of NOX1 and NCF1 in cortical neurons ... The activation of PKA for 24 h induced the transcriptional upregulation of NOX1 and NCF1 in cortical neurons
Fang et al., J Cell Biochem 2012 : Based on our findings, we suggest that Mob2 is involved in PKA signaling mediated astrocytic stellation
Fujii et al., Life Sci 2012 : Activation of PKC and PKA up-regulates ACh synthesis in T cells, and immunological activation triggers ChAT gene transcription through calcineurin mediated pathways
Song et al., Int J Mol Med 2012 : The phosphorylation levels of nNOS at Ser1412, induced by activation of Akt/PKB or CaM-K II, but not by PKA signaling, were reduced by pre-treatment with the NO donor diethylamine-NONOate ... Furthermore, the levels of phosphorylation of nNOS at Ser1412, induced by Akt/PKB or CaM-K II but not by PKA signaling, were enhanced by inhibition of nNOS activity with 7-nitroindazole
Koçer et al., Journal of molecular signaling 2012 : Using an AKAR2-AKAP5 fusion `` biosensor '', we investigated the spatial-temporal activation of AKAP5 undergoing phosphorylation by PKA in response to ß-adrenergic stimulation
Lee et al., J Biol Chem 2012 (MAP Kinase Signaling System...) : PDE4B inhibits MKP-1 expression in a cAMP-PKA dependent manner
Wang et al., J Biol Chem 2012 : Activation of ezrin anchored PKA initiates NHERF1 phosphorylation to disassemble the ternary complex, release Npt2a, and thereby inhibit phosphate transport
Liu et al., Eur J Pharmacol 2012 (Calcium Signaling) : This negative effect was well correlated with increased cAMP levels via PKA activity and the subsequent inhibition of ERK ( p42/p44 ) phosphorylation to decrease superoxide anion production
Spirli et al., Hepatology 2012 (Cysts...) : Cyclic AMP/PKA dependent paradoxical activation of Raf/MEK/ERK signaling in polycystin-2 defective mice treated with sorafenib ... These effects are consistent with the ability of Raf inhibitors to transactivate Raf-1 when a PKA activated Ras promotes Raf-1/B-Raf heterodimerization, and are inhibited by interfering with cAMP/PKA signaling both in vitro and in vivo, as shown by the reduction of liver cysts in mice treated with combined octreotide and sorafenib ... These effects are consistent with the ability of Raf inhibitors to transactivate Raf-1 when a PKA activated Ras promotes Raf-1/B-Raf heterodimerization, and are inhibited by interfering with cAMP/PKA signaling both in vitro and in vivo, as shown by the reduction of liver cysts in mice treated with combined octreotide and sorafenib
Li et al., Acta Pharmacol Sin 2012 (Myocardial Infarction...) : The aim of this study was to examine whether protein kinase A (PKA) was involved in statin mediated eNOS phosphorylation and cardioprotection ... SIM significantly increased the activities of PKA and constitutive NOS, and increased Ser ( 133 ) p-CREB protein, Ser ( 1179 ) p-eNOS, and Ser ( 635 ) p-eNOS in ischemic myocardium
Nedvetsky et al., Mol Biol Cell 2012 : cAMP dependent apoptosis is accompanied by a posttranscriptional PKA dependent increase in the proapoptotic protein Bcl-2 interacting mediator of cell death
Lieskovska et al., Parasite Immunol 2012 : Saliva induced enhancement of IL-10 was not observed in the presence of specific inhibitor of Protein Kinase A (PKA) , H-89, suggesting the involvement of PKA pathway in IL-10 production
Matsumoto et al., Cell Res 2012 (Calcium Signaling) : A recent report in Nature showed that PKA dependent phosphorylation of the inositol-1,4,5-trisphosphate receptor ( InsP3R ) induces Ca mobilization, leading to increase in the phosphatase activity of calcineurin and the subsequent dephosphorylation of CRTC2, thereby resulting in the induction of gluconeogenic gene expression
Lee et al., PloS one 2012 (Neurodegenerative Diseases) : In this study, we show that one cytokinin, zeatin riboside, can prevent pheochromocytoma ( PC12 ) cells from serum deprivation induced apoptosis by acting on the adenosine A(2A) receptor ( A ( 2A ) -R ), which was blocked by an A ( 2A ) -R antagonist and a protein kinase A (PKA) inhibitor, demonstrating the functional ability of zeatin riboside by mediating through A ( 2A ) -R signaling event
Kisslov et al., Biochim Biophys Acta 2012 (Colonic Neoplasms) : AS or H-89, but not Ly294002, inhibited CREB activation, suggesting that CREB activation is mediated by PKA
Edwards et al., Biochem J 2012 : Gene silencing and rescue experiments establish that AKAP-Lbc mediated PKA phosphorylation of Hsp20 is crucial to the anti-apoptotic effects of the Hsp
Joshi et al., Frontiers in physiology 2012 : Interestingly, stimulation of Cx43 expression by DADS was not dependent on PKA , PKG or PKC
Rosa et al., J Physiol 2012 (Anoxia...) : To address this question we measured the early effects of hypoxia ( 5-60 s, P ( O ( 2 ) ) < 5 mmHg ) on Ca ( 2+ ) current ( I ( Ca ) ) and tested the involvement of protein kinase A (PKA) phosphorylation, Ca ( 2+ ) /calmodulin mediated signalling and the haem oxygenase ( HO ) pathway in the hypoxic regulation of Ca(V)1.2 in rat and cat ventricular myocytes and HEK-293 cells
Wang et al., BMC cancer 2012 : PKA activation in TG2 overexpressing MEF ( tg2-/- ) cells resulted in an increased activation of NF-?B and Akt phosphorylation in comparison to empty vector transfected control cells as determined by the reporter-gene assay and immunoblot analysis respectively
Voss et al., Inflamm Allergy Drug Targets 2012 : While poly ( I:C ) induced IL-8 gene expression was solely inhibited by the NF-?B inhibitor III, MCP-1 gene induction was also blocked by PKA , p38 MAPK and JAK-STAT inhibitors ... While poly ( I:C ) induced IL-8 gene expression was solely inhibited by the NF-?B inhibitor III, MCP-1 gene induction was also blocked by PKA , p38 MAPK and JAK-STAT inhibitors
Williams et al., J Neurosci 2012 : Since secretin receptors activate PKA ( Wessels-Reiker et al., 1993 ), we tested the hypothesis that secretin regulates Kv1.2 trafficking in the cerebellum
Park et al., Neurochem Int 2012 : Furthermore, HO-1 activation via PKA mediated CREB phosphorylation attenuated the expression of neuroinflammatory molecules in LPS induced microglial cells
Li et al., J Biol Chem 2012 (Long QT Syndrome) : Human mutations that disrupt I ( Ks ) -Yotiao interaction result in reduced PKA dependent phosphorylation of the I ( Ks ) subunit KCNQ1 and inhibition of sympathetic stimulation of I ( Ks ), which can give rise to long-QT syndrome
Narasimamurthy et al., Proc Natl Acad Sci U S A 2012 (Inflammation) : We further demonstrate that increased phosphorylation of p65 at S276 residue in Cry1 ( -/- ) ; Cry2 ( -/- ) cells is due to increased PKA signaling activity, likely induced by a significantly high basal level of cAMP, which we detected in these cells
Stückemann et al., Development 2012 : We show that Cxcr4a inhibits PKA activity independently of Hh signalling, and propose that Cxcr4a enhances Hh-dependent proliferation by promoting the activity of Gli1
Yates-Binder et al., PloS one 2012 : Inhibition of endothelial function by IP-10p was similar to that described for IP-10, secondary to CXCR3 mediated increase in cAMP production, activation of PKA inhibiting cell migration, and inhibition of VEGF mediated m-calpain activation ... Inhibition of endothelial function by IP-10p was similar to that described for IP-10, secondary to CXCR3 mediated increase in cAMP production, activation of PKA inhibiting cell migration, and inhibition of VEGF mediated m-calpain activation ... Inhibition of endothelial function by IP-10p was similar to that described for IP-10, secondary to CXCR3 mediated increase in cAMP production, activation of PKA inhibiting cell migration, and inhibition of VEGF mediated m-calpain activation
Li et al., Int J Cardiol 2013 : Ischemia or ischemia-reperfusion induced severe myocardial edema, PKA activation , and up-regulation of aquaporin-1 , -4, -8, and -9 in the reflow and no-reflow myocardium ... The cardioprotective effects of acute SIM therapy against myocardial no-reflow and infarction relate to the reduction of myocardial edema by suppressing the expression of aquaporin-1 , -4, -8, and -9 in a partially PKA dependent manner
Nascimento et al., Eur J Pharmacol 2012 : Relaxin induced AKT phosphorylation was G ( i ) - but not PKA dependent , and it was blocked by both PI3K and MEK inhibitors ... Relaxin induced AKT phosphorylation was G ( i ) - but not PKA dependent , and it was blocked by both PI3K and MEK inhibitors
He et al., PloS one 2012 : Inhibition of G ( i ) eliminated the CPH induced effect on both the I ( K ) and PKA
Zhou et al., J Ethnopharmacol 2012 (Melanoma, Experimental) : The special p38 MAPK inhibitor, SB203580, and protein kinase A (PKA) inhibitor, H89, both attenuated the AVE induced up-regulation of MITF and tyrosinase expression in B16F10 cells and NHMC ... The special p38 MAPK inhibitor, SB203580, and protein kinase A (PKA) inhibitor, H89, both attenuated the AVE induced up-regulation of MITF and tyrosinase expression in B16F10 cells and NHMC
Chen et al., J Neurosci 2012 (Neuroblastoma) : Here, we found that overexpression of BACE1 reduces cAMP response element binding protein ( CREB ) phosphorylation, protein kinase A (PKA) activity, and cAMP levels, whereas downregulation of BACE1 has the opposite effect
Loilome et al., Tumour Biol 2012 (Adenocarcinoma...) : We hypothesize a role for protein kinase A (PKA) in Ov-induced CCA
Sekiguchi et al., Br J Pharmacol 2013 (Hyperalgesia) : In NG108-15 cells and/or rat DRG neurons, dibutyryl cAMP ( db-cAMP ) or PGE ( 2 ) increased T currents, an effect blocked by AKAP St-Ht31 inhibitor peptide ( AKAPI ) or KT5720, a PKA inhibitor
Birnbaum et al., Cardiovasc Drugs Ther 2012 (Diabetes Mellitus, Type 2...) : MK-2 and Cil increased PKA activity when given alone ; however, PKA activity was significantly higher in the MK-2+Cil group than in the other groups
Fernández-Galilea et al., J Lipid Res 2012 : LA treatment also induced a marked increase in PKA mediated perilipin phosphorylation
Lee et al., Am J Physiol Endocrinol Metab 2012 (Insulin Resistance) : Cotreatment with DEX blocked TNF induction of basal lipolysis and insulin resistance by antagonizing TNF stimulation of PKA mediated phosphorylation of hormone-sensitive lipase (HSL) at Ser ( 563 ) and Ser ( 660 ) and perilipin ... Cotreatment with DEX blocked TNF induction of basal lipolysis and insulin resistance by antagonizing TNF stimulation of PKA mediated phosphorylation of hormone-sensitive lipase (HSL) at Ser ( 563 ) and Ser ( 660 ) and perilipin
Lessa et al., American journal of physiology. Renal physiology 2012 : Overall, our data suggest that the inhibitory effect of UGN on NHE3 transport activity in proximal tubule is mediated by activation of both cGMP/PKG and cAMP/PKA signaling pathways which in turn leads to NHE3 phosphorylation and reduced NHE3 surface expression
Shaikh et al., Cell Signal 2012 (Lung Neoplasms) : Inhibition of PKA activity with chemical inhibitors prevented EMT induced by hypoxia and tumor growth factor ß1 ... However, activation of PKA by forskolin and 8-Br-cAMP did not induce EMT ... Our results suggest that hypoxia enhances PKA activity by upregulating PKA gene expression in a HIF dependent mechanism and that PKA plays a key role in hypoxia mediated EMT , migration, and invasion in lung cancer cells
Castellani et al., Lab Invest 2012 (Cystic Fibrosis) : Na ( + ) /H ( + ) exchanger regulatory factor 1 ( NHERF1 ) is a protein involved in PKA dependent activation of CFTR by interacting with CFTR via its PDZ domains and with ezrin via its C-terminal domain
Su et al., PloS one 2012 : In the present studies, we have demonstrated that PKA enhances NF-E2 SUMOylation in an in vitro system using purified proteins, suggesting a possible mechanism for PKA dependent activation of the NF-E2 transcription factor through SUMOylation ... In the present studies, we have demonstrated that PKA enhances NF-E2 SUMOylation in an in vitro system using purified proteins, suggesting a possible mechanism for PKA dependent activation of the NF-E2 transcription factor through SUMOylation
Shapiro et al., PloS one 2012 : Sgt1 physically interacts with Cyr1, and depletion of either Sgt1 or Hsp90 activates cAMP-PKA signaling, revealing the elusive link between Hsp90 and the PKA signaling cascade ... Sgt1 physically interacts with Cyr1, and depletion of either Sgt1 or Hsp90 activates cAMP-PKA signaling, revealing the elusive link between Hsp90 and the PKA signaling cascade
Dezaki et al., Methods Enzymol 2012 : Ghrelin attenuates the glucose induced cAMP production and PKA activation, which drives activation of Kv channels and suppression of the glucose induced [ Ca ( 2+ ) ] ( i ) increase and insulin release in ß-cells
Ying et al., FEBS Lett 2012 (Synaptic Transmission) : Knock-down of syntabulin by shRNA reduced both basal and glucose stimulated insulin secretion, and diminished cAMP-Epac2 and cAMP-PKA potentiated insulin secretion
Yang et al., Cardiovasc Res 2013 (Disease Models, Animal...) : Early ischaemic preconditioning requires Akt- and PKA mediated activation of eNOS via serine1176 phosphorylation ... Thus, Akt- and PKA mediated eNOS activation, with phosphorylation near the C-terminus, is critical for early IPC induced cardioprotection, with eNOS derived NO from the endothelium serving a critical role
Kocieda et al., J Biol Chem 2012 (Second Messenger Systems) : Mutation within the putative CREB and C/EBP sites combined with in vivo DNA binding ( ChIP ) assays identified the distal CREB site ( -1125 ) and the two proximal C/EBP sites ( -274 and -232 ) as essential for PKA activated CREB and EPAC activated C/EBPß induced IL-23p19 expression ... Mutation within the putative CREB and C/EBP sites combined with in vivo DNA binding ( ChIP ) assays identified the distal CREB site ( -1125 ) and the two proximal C/EBP sites ( -274 and -232 ) as essential for PKA activated CREB and EPAC activated C/EBPß induced IL-23p19 expression ... Using the EP4 agonist PGE ( 1 ) OH in conjunction with TNFa, we found that PKA induced phosphorylation of cAMP-response element binding protein ( ( P ) CREB ) and EPAC induced phosphorylation of C/AATT enhancer binding protein ß ( ( P ) C/EBPß ) mediate the stimulatory effect of PGE2 on IL-23p19 expression
Sun et al., Peptides 2012 : H89, a selective inhibitor of cyclic AMP dependent protein kinase A (PKA), inhibited the NPFF activated ERK pathway, indicating the involvement of PKA in the NPFF induced ERK activation
Zhu et al., Exp Diabetes Res 2012 (Diabetic Nephropathies...) : In conclusion, low dosage/concentration Glib prevents HG-induced collagen accumulation in MC by enhancing collagen catabolism in a cAMP-PKA mediated PAI-1 inhibition
Oishi et al., J Biol Chem 2012 : Protein kinase A (PKA) inhibits RhoA signaling and thereby induces a characteristic morphological change, cell rounding ... Here we show that phosphorylation of RhoGDIa but not RhoA plays an essential role in the PKA induced inhibition of RhoA signaling and in the morphological changes using cardiac fibroblasts
Manchanda et al., Oncogene 2013 (Neurilemmoma) : Further, PKA activation through mutation in Prkar1a is sufficient to initiate Rac1 signaling, with subsequent reduction of Nf2 and schwannomagenesis
Burmeister et al., J Biol Chem 2012 (Cardiomegaly) : Our data indicate that AKAP-Lbc integrates PKA and Shp2 signaling in the heart and that AKAP-Lbc associated Shp2 activity is reduced in hypertrophic hearts in response to chronic ß-adrenergic stimulation and PKA activation
Wang et al., Bone 2013 : NHERF1 regulation of PTH-sensitive Pi uptake in proliferating osteoblasts is mediated by cAMP/PKA and PLC/PKC, while modulation of Pi uptake in differentiated osteoblasts depends only on cAMP/PKA signaling
Ma et al., J Biol Chem 2012 (Atherosclerosis) : We present evidence that ABCA1 activates PKA and that this elevated PKA activity contributes to M2-like inflammatory response from ABCA1 ( +/+ ) bone marrow derived macrophages
Maymó et al., PloS one 2012 (MAP Kinase Signaling System) : We found that specific PKA inhibition by H89 caused a significant increase of hCG leptin induction, suggesting that probably high cAMP levels might inhibit hCG effect ... In this work, we demonstrated that hCG leptin induction through the MAPK signaling pathway is inhibited by PKA
Wang et al., J Biol Chem 2012 : First, not only D835A, D836A, and E838A but also K946A reduced the PKA dependent CFTR activation
Wang et al., Exp Neurol 2013 : Involvement of PKA dependent upregulation of nNOS-CGRP in adrenomedullin initiated mechanistic pathway underlying CFA induced response in rats ... Involvement of PKA dependent upregulation of nNOS-CGRP in adrenomedullin initiated mechanistic pathway underlying CFA induced response in rats
Queiroz et al., PloS one 2012 (Leukemia) : Pro-death activity of violacein is actually carried out by inhibition of calpain and DAPK1 and activation of PKA , AKT and PDK, followed by structural changes caused by endoplasmic reticulum stress and Golgi apparatus collapse, leading to cellular demise ... Pro-death activity of violacein is actually carried out by inhibition of calpain and DAPK1 and activation of PKA , AKT and PDK, followed by structural changes caused by endoplasmic reticulum stress and Golgi apparatus collapse, leading to cellular demise
Balwani et al., PloS one 2012 : Thus, MPTAG mediated inhibition of TNF-a induced Akt activation was independent of PI-3K and dependent on PKA ... Most importantly, MPTAG restores the otherwise repressed activity of PKA and inhibits the TNF-a induced Akt phosphorylation at both Thr308 and Ser473 residues
Campo et al., Biofactors 2012 (Inflammation) : IL-1beta stimulation significantly up-regulated HA levels, its fragmentation, cAMP, PKA , cytokine levels, and activated NF-kB
Ye et al., Am J Physiol Heart Circ Physiol 2013 (Diabetes Mellitus, Type 2...) : Glucagon-like peptide (GLP)-1 receptor activation increases intracellular cAMP with downstream activation of PKA ... Glucagon-like peptide (GLP)-1 receptor activation increases intracellular cAMP with downstream activation of PKA
Eisel et al., Biochem Pharmacol 2013 : Taken together, we demonstrate a redox mediated activation of PKA by PDGF-BB thus highlighting a physiological role of ROS as regulator of PKA activity in rat mesangial cells
Stone et al., Frontiers in physiology 2012 : Interestingly, AICAR also phosphorylated a suspected PKA-inhibitory Ser485 site on AMPK, and these cumulative events were reversed by the PKA inhibitor PKI suggesting possible PKA mediated regulation of AMPK ... The cAMP stimulator forskolin increased PKA activity and completely ameliorated Ser/Thr protein phosphatase-2C activity, suggesting a potential mechanism of AMPK modulation by PKA since inhibition of PKA by PKI reduced AMPK activity
Liu et al., J Mol Cell Cardiol 2013 (Cardiomyopathies) : Reducing mitochondrial ROS by application of NAD ( + ), mitoTEMPO, PKC inhibitors, or PKA activators, restored I(Na)
Choi et al., J Cell Physiol 2013 (Prostatic Neoplasms) : We demonstrated that the Serine-70 of NCoR is identified the critical amino acid for PKA dependent NCoR phosphorylation ... More importantly, the activation of PKA enhanced the repressive activity of NCoR in a reporter assay and potentiated the antagonist activity in the Androgen Receptor (AR) mediated transcription
Lee et al., Nat Biotechnol 2012 (Dysautonomia, Familial) : One of the hits, SKF-86466, was found to induce IKBKAP transcription through modulation of intracellular cAMP levels and PKA dependent CREB phosphorylation
Chang et al., J Biol Chem 2013 : Isoproterenol ( ISO ) or PKA activation results in strong nuclear accumulation of HDAC5 in contrast to nuclear export driven by Ca ( 2+ ) -calmodulin protein kinase II and protein kinase D
Makinodan et al., Exp Dermatol 2012 (Carcinoma, Basal Cell...) : Here we show that PKA activation via the cAMP agonist forskolin is sufficient to completely abolish oncogenic Smo activity in vitro
Khan et al., Gen Comp Endocrinol 2013 : In addition, inhibition of endogenous PKA by a more selective peptide inhibitor [ PKI- ( 6-22 ) -amide ] was sufficient to resume GVBD and MAPK activation in intact perch oocytes
Zhang et al., PloS one 2012 : We and others also have shown that the high glucose induced decrease in G6PD activity is mediated , at least in part, by cAMP dependent protein kinase A (PKA)
Dong et al., Am J Physiol Endocrinol Metab 2013 : In cultured endothelial cells, GLP-1 acutely increased PKA activity and stimulated endothelial NO synthase phosphorylation at Ser ( 1177 ) and NO production ... In cultured endothelial cells, GLP-1 acutely increased PKA activity and stimulated endothelial NO synthase phosphorylation at Ser ( 1177 ) and NO production
Luo et al., Int J Biochem Cell Biol 2013 (Second Messenger Systems) : It is of note that GLP-1 preferentially increased the expression and activity of PKA , whereas had no effects on Epac2 at high glucose
Tu et al., PloS one 2012 : Our data suggest that CFTR regulated release contributes to ATP release from contracting muscle in vivo, and that cAMP and PKA are involved in the activation of CFTR during muscle contractions or acidosis ; NHE and NCX may be involved in the signal transduction pathway
Dringenberg et al., Horm Metab Res 2013 : Activation of the MC2R increases stimulatory G-protein, adenylyl cyclase, and protein kinase A (PKA) activities
Havekes et al., J Neurosci 2012 : Furthermore, both hippocampal ß2-adrenergic receptor phosphorylation by PKA , and learning induced activation of ERK in the CA1 region of the hippocampus are attenuated in mice lacking gravin-a
Yang et al., Small GTPases 2013 : Recent observations reveal that Plexin 's GAP activity is controlled by the cAMP dependent protein kinase (PKA) , which phosphorylates Plexin and generates a binding site for the phospho-serine/threonine binding protein 14-3-3e
Jia et al., Endocrinology 2013 : Inhibition of PKA significantly attenuated the effect of genistein on thrombin induced EC permeability, MLC phosphorylation, and RhoA membrane translocation in ECs ... These findings demonstrated that genistein improves thrombin induced endothelial barrier dysfunction in ECs through PKA mediated suppression of RhoA signaling
Strainic et al., Nat Immunol 2013 : Here we found that when signals from C3aR and C5aR were not transduced into CD4 ( + ) cells, signaling via the kinases PI(3)K?, Akt and mTOR ceased, activation of the kinase PKA increased , autoinductive signaling by transforming growth factor-ß1 ( TGF-ß1 ) initiated and CD4 ( + ) T cells became Foxp3 ( + ) induced regulatory T cells ( iT ( reg ) cells )
Chen et al., Cell Mol Neurobiol 2013 : Consistent with the involvement of PKA in the modulation of PAR4 expression, this evoked expression both at mRNA and protein levels was significantly inhibited after PKA was inhibited by pre-incubation with PKI14-22
Rajagopal et al., Am J Physiol Gastrointest Liver Physiol 2013 : OA, but not a selective exchange protein activated by cAMP ( Epac ) ligand ( 8-pCPT-2'-O-Me-cAMP ), caused phosphorylation of RhoA and the phosphorylation was blocked by the PKA inhibitor, myristoylated PKI, and by the expression of phosphorylation-deficient mutant RhoA ( S188A ) ... We conclude that activation of TGR5 causes relaxation of gastric smooth muscle and the relaxation is mediated through inhibition of RhoA/Rho kinase pathway via both cAMP/Epac dependent stimulation of Rap1 and cAMP/PKA dependent phosphorylation of RhoA at Ser ( 188 )
Kobayashi et al., Arterioscler Thromb Vasc Biol 2013 : This phenomenon is mediated by cAMP/PKA/Tiam1 dependent Epac1 independent Rac1 activation and subsequent enhancement of adherens junction in endothelial cell
Schweigel et al., Proteomics 2013 : Given the fact that PKA activity is negatively regulated by the P2Y12 receptor, our data provide evidence for a novel link of synergistic control of the state of tyrosine phosphorylation by both P2Y receptors
Jin et al., Exp Cell Res 2013 : Taken together, our results demonstrate that TGFß induction of PKA activity results in phosphorylation of km23-1 on S73
Rodriguez et al., J Biol Chem 2013 : Taken together, these findings suggest that Fsk activation of adenylate cyclase and PKA can negatively regulate IL-2 signaling at multiple levels that include IL-2R complex formation and Jak3/Stat5 activation
Kaihara et al., Diabetes 2013 (Hyperglycemia...) : In response to sustained hyperglycemia, PKA activity potentiated both acute and sustained insulin release ... Intracellular calcium signaling was unaffected by PKA activation, suggesting that the effects of PKA on acute-phase insulin secretion are mediated by the phosphorylation of proteins involved in ß-cell exocytosis
He et al., PloS one 2013 : Cyclooxygenase inhibitors and PGE ( 2 ) receptor ( EP ) antagonists were able to eliminate this ELF-EMF induced increase in phosphorylated PKA and I(Na)
Por et al., Biochem J 2013 : Pharmacological activation of the kinases PKA ( protein kinase A ) and PKC ( protein kinase C ) led to a robust increase in TRPV1 and ß-arrestin-2 association, whereas inhibition of PKA and PKC decreased association
Manna et al., J Biol Chem 2013 (Second Messenger Systems) : These findings provide novel insight and demonstrate the molecular events by which HSL acts to drive cAMP/PKA mediated regulation of StAR expression and steroidogenesis in mouse Leydig cells
Dacher et al., J Neurosci 2013 : Using immunofluorescence and whole-cell patch-clamp recording in rat midbrain slices, we show that activation of postsynaptic D ( 2 ) -like family of dopamine ( DA ) receptor in the ventral tegmental area ( VTA ) induces long-term depression ( LTD ) of GABAergic synapses on DA neurons through an inositol triphosphate receptor mediated local rise in postsynaptic Ca ( 2+ ) and CaN activation accompanied by PKA inhibition, which requires AKAP150 as a bridging signaling molecule
Thompson et al., Mol Endocrinol 2013 : GnRH stimulation of CREB phosphorylation ( pCREB ) in the gonadotrope derived LßT2 cell line was attenuated by a protein kinase A (PKA) inhibitor, H89 ... GnRH stimulation of CREB phosphorylation ( pCREB ) in the gonadotrope derived LßT2 cell line was attenuated by a protein kinase A (PKA) inhibitor, H89
Spahn et al., Pain 2013 (Disease Models, Animal...) : Inhibition of AC and PKA , as well as mutations of the PKA phosphorylation sites threonine 144 and serine 774, prevented the enhanced TRPV1 activity
Li et al., J Neurosci 2013 : Cx36 among photoreceptor terminals, representing predominantly rod-cone gap junctions but possibly including some rod-rod and cone-cone gap junctions, was phosphorylated in a PKA dependent manner by the same treatments
Ji et al., Molecular pain 2013 (Pain...) : CRF increased a latent NMDA receptor mediated EPSC, and this effect also required CRF1 and PKA but not CRF2 and PKC
Shell et al., Biochim Biophys Acta 2013 : In the process of validating this assay, we discovered that PKA activity is regulated by the protease calpain
Jia et al., Cell Signal 2013 : Furthermore, Eph induced PKA mediated cyclic AMP response element binding protein ( CREB ) phosphorylation
Shan et al., J Gastroenterol Hepatol 2013 : Inhibition of PKA did not block the bile acid induced p38 MAPK activation
Guereschi et al., Eur J Immunol 2013 : B2AR activation in Treg cells leads to increased intracellular cAMP levels and to protein kinase A (PKA) dependent CREB phosphorylation
Hoang et al., Endocrinology 2013 : Here we show that the cAMP response element binding protein ( CREB ) downregulates GRIP1 and is necessary for the PKA stimulated degradation of GRIP1 , which leads to changes in the expression of a subset of genes regulated by estrogen receptor-a in MCF-7 breast cancer cells ... We propose that CREB mediates the PKA stimulated degradation of GRIP1 through protein-protein interaction and stimulation of proteasomal degradation of ubiquitinated GRIP1
Roy et al., Mol Biol Cell 2013 : Mth1 regulates the interaction between the Rgt1 repressor and the Ssn6-Tup1 corepressor complex by modulating PKA dependent phosphorylation of Rgt1
Jana et al., J Neuroimmune Pharmacol 2013 : NaB induced the activation of protein kinase A (PKA) , but not protein kinase C ( PKC ), and H-89, an inhibitor of PKA, abrogated NaB induced increase in neurotrophic factors
Ombra et al., Biochim Biophys Acta 2013 (Breast Neoplasms...) : Retinoic acid impairs estrogen signaling in breast cancer cells by interfering with activation of LSD1 via PKA
Wu et al., Eur J Pharmacol 2013 : Using F11 cells as a model of post-synaptic neurons, the present study examined the role of protein kinase C ( PKC ) and protein kinase A (PKA) in desensitization of the 5-HT1A-receptor by agonist
Fang et al., American journal of physiology. Renal physiology 2013 (Renal Insufficiency, Chronic) : Adiponectin did not activate PKA in renal tubular cells, and the specific PKA inhibitor myristoylated PKI ( 14-22 ) amide failed to block the inhibitory effect of adiponectin on ANG II-induced NADPH oxidase activation
Zhang et al., Invest Ophthalmol Vis Sci 2013 : Our findings suggest Compound 49b induces DNA-PK levels through PKA activity
Rashid et al., J Neurochem 2013 : Conversely, PKA inhibitors or PKA knockdown abolished the synaptamide induced neuronal differentiation of NSCs. URB597, a fatty acid amide hydrolase (FAAH) inhibitor, elevated the level of DHA derived synaptamide and further potentiated the DHA- or synaptamide induced neuronal differentiation of NSCs
Dalton et al., Cell Signal 2013 : FAK 397 Tyr-P and Phase I FAK 576/577 Tyr-P involved protein tyrosine phosphatase ( PTP1B and Shp1/Shp2 ) -mediated Src activation, Protein Kinase A (PKA) inhibition , and integrin activation
Holleran et al., J Cell Sci 2013 : Stimulation by protein kinase A (PKA) depleted this intracellular pool and redistributed CFTR channels to the cell surface, elicited by reduced endocytosis and active translocation to the PM. Corrector or temperature rescue of F508del also resulted in targeting to the ERC and exhibited subsequent PKA stimulated trafficking to the PM. Corrector treatment ( 24 hours ) led to persistent residence of F508del in the ERC, while thermally destabilized F508del was targeted to lysosomal compartments by 3 hours
Kach et al., Am J Physiol Lung Cell Mol Physiol 2013 (Pulmonary Fibrosis) : TGF-ß stimulated a profound expression of CRLR with a time course that mirrored the increased PKA responses to ADM ... The TGF-ß receptor kinase inhibitor SB431542 abolished expression of CRLR and attenuated the PKA responses of cells to ADM but not to iloprost
D'Inzeo et al., Int J Oncol 2013 : Our data show that the Smad4 C324Y mutation interacts with P-Smad3 more strongly than Smad4 wt, already in basal condition ; this interaction is responsible for TGF-ß signaling and PKA activation that, in turn, determines an increased phosphorylation of CREB , necessary for the mitogenic actions of TSH
Franzellitti et al., PloS one 2013 : FX significantly decreased cAMP levels and PKA activity, and induced ABCB mRNA down-regulation ... CONCLUSIONS : This study provides the first direct evidence for the cAMP/PKA mediated regulation of ABCB transcription in mussels
Subramanian et al., Journal of thrombosis and haemostasis : JTH 2013 (Calcium Signaling) : Phosphorylation of S587 correlated with the inhibitory effect of PKA on Rap1b activation in platelets
Bäck et al., J Mol Endocrinol 2013 : However, inhibition of PKA did not affect the regulation of MT1X by TSH ... In summary, these findings indicate that TSH stimulation induces MT1X expression via Gq/11 and PKC, whereas cAMP-PKA signaling does not play a predominant role
Neukamm et al., J Biol Chem 2013 : Phosphorylation of IRS2 on PKA consensus motifs was induced by forskolin and the PKA activator N ( 6 ) -Phe-cAMP and prevented by both PKA inhibitors
Zheng et al., Journal of the American Heart Association 2013 (Calcium Signaling) : AA dilates HCAs through a novel mechanism involving endothelial TRPV4 channel dependent Ca ( 2+ ) entry that requires endothelial hyperpolarization, PKA mediated basal phosphorylation of TRPV4 , and direct activation of TRPV4 channels by AA
Xu et al., Life Sci 2013 (MAP Kinase Signaling System) : PKA and ERK1/2 are involved in dopamine D1 receptor induced heterologous desensitization of the d opioid receptor
Wiseman et al., J Biol Chem 2013 (Second Messenger Systems) : Proteasomal Degradation of Eukaryotic Elongation Factor-2 Kinase ( EF2K ) Is Regulated by cAMP-PKA Signaling and the SCFßTRCP Ubiquitin E3 Ligase ... Proteasomal Degradation of Eukaryotic Elongation Factor-2 Kinase ( EF2K ) Is Regulated by cAMP-PKA Signaling and the SCFßTRCP Ubiquitin E3 Ligase
Bonito-Oliva et al., Neuropharmacology 2013 : They also indicate that this effect is exerted by suppressing dephosphorylation at Ser240/244, through PKA dependent activation of DARPP-32 and inhibition of PP-1 ... They also indicate that this effect is exerted by suppressing dephosphorylation at Ser240/244, through PKA dependent activation of DARPP-32 and inhibition of PP-1
Chiluiza et al., J Biol Chem 2013 (Glomerulosclerosis, Focal Segmental) : However, these effects appear to be independent of their effects on calcium/calmodulin dependent protein kinase II and PKA , respectively
Bachmann et al., Proc Natl Acad Sci U S A 2013 (MAP Kinase Signaling System) : Our findings offer additional mechanistic insights how ß-adrenergic receptor controlled PKA activities enhance GTP-Rac mediated activation of nuclear Erk1/2 signaling ... Our findings offer additional mechanistic insights how ß-adrenergic receptor controlled PKA activities enhance GTP-Rac mediated activation of nuclear Erk1/2 signaling
Mighiu et al., Nat Med 2013 : Glucagon activates hepatic protein kinase A (PKA) to increase glucose production, but the gluco-stimulatory effect is transient even in the presence of continuous intravenous glucagon infusion
Guibinga et al., PloS one 2013 : In the present studies, we find that HPRT-deficient neuronal cell lines have reduced CREB ( cAMP response element binding protein ) expression and intracellular cyclic AMP ( cAMP ), which correlates with attenuated CREB dependent transcriptional activity and a reduced phosphorylation of protein kinase A (PKA) substrates such as synapsin ( p-syn I )
Cheng et al., DNA Cell Biol 2013 : Nevertheless, the effect of PKA inhibition on the HCN channel activity in DRG neuron remains to be elucidated
Shima et al., Cancer Res 2013 : PKA mediated phosphorylation of PML-RARa blocked its ability to inhibit PML oligomerization and destabilize HIPK2
Choi et al., Biochem Biophys Res Commun 2013 (Inflammation) : PKA negatively regulates PP2Cß to activate NF-?B mediated inflammatory signaling
Billet et al., J Biol Chem 2013 : Results suggest that PKA and tyrosine kinases contribute to CFTR regulation by GPCRs that are expressed at the apical membrane of intestinal and airway epithelia
Aouida et al., PloS one 2013 : Further, we demonstrate agonist-AdipoR dependent activation of protein kinase A (PKA) signaling and AMP activated protein kinase (AMPK) phosphorylation in S. cerevisiae, which are homologous to important mammalian adiponectin-AdipoR1 signaling pathways
Moberly et al., Basic Res Cardiol 2013 (Diabetes Mellitus, Type 2...) : No evidence for GLP-1 mediated activation of cAMP/PKA or AMPK signaling in lean or obese hearts was observed
Lallet-Daher et al., Cancer Res 2013 : Restoring KCNA1 expression in transformation-competent cells triggered variation in membrane potential and blocked RAS induced transformation, and PKA activation suppressed both effects
Larabee et al., J Biol Chem 2013 : ET as well as a cell-permeable activator of PKA induce Notch target genes ( HES1, HEY1, IL2RA, and IL7R ) and are able to significantly enhance the induction of these Notch target genes by a Toll-like receptor ligand
Schott et al., Cell Signal 2013 : Finally, confocal microscopy of cells co-transfected with gravin-EYFP and PKA RII-ECFP revealed that gravin redistribution mediated by ionomycin, thapsigargin, and ATP each triggered the gravin dependent loss of PKA localized at the cell cortex
Cabral et al., J Pharmacol Exp Ther 2013 (Colonic Neoplasms) : A significant increase in cAMP levels ( 32 ± 3 % and 14 ± 2 % after short- and long-term stimulation, respectively ) was detected, and inhibition of protein kinase A (PKA) , phospholipase C (PLC), and downregulation of protein kinase C ( PKC ) prevented NHE1 inhibition
Hillman et al., PloS one 2013 : Moreover, inhibition of PKA activity by H89, or its activation using 8Br-cAMP, increased AKAP3 degradation rate
Patrussi et al., Frontiers in immunology 2013 : We have shown that exogenous GroPIns4P enhances CXCL12 induced T-cell chemotaxis through activation of the kinase Lck in a cAMP/PKA dependent manner
Fenne et al., PloS one 2013 : In order to identify genes that may be regulated through PKA induced downregulation of SRC-2 , overlapping transcriptional targets in response to the respective treatments were characterized
Duff et al., PloS one 2013 : The CB2R induced morphological changes observed at the growth cone are PKA dependent and require the presence of the netrin-1 receptor, Deleted in Colorectal Cancer
Hu et al., PloS one 2013 : Although stretch activated the kinases AMPKa, PKA, Akt, and ERK1/2, stretch induced eNOS activation was only inhibited by kinase-specific inhibitors of PKA and PI3K/Akt, but not of AMPKa and Erk1/2 ... Furthermore, inhibition of PKA preferentially attenuated eNOS activation in the early phase, while inhibition of the PI3K/Akt pathway reduced eNOS activation in the late phase, suggesting that the PKA and PI3K/Akt pathways play distinct roles in a time dependent manner
Lee et al., Allergy, asthma & immunology research 2013 : LPS induced COX-2 expression in RAW 264.7 macrophages was enhanced by exogenous PGE2 or cyclic AMP ( cAMP ) analogue and was suppressed by a COX inhibitor ( indomethacin ), a protein kinase A (PKA) inhibitor ( KT5720 ), and A kinase anchoring protein ( AKAP ) disruptors ( Ht31 and RIAD )
Bringhurst et al., Mol Endocrinol 1989 (Osteosarcoma) : In the absence of metallothionein induction, these cells exhibited nearly normal PTH responsiveness, but after REV-1 induction by Zn2+, they were resistant to PTH induced activation of PK-A and regulation of membrane phospholipid synthesis by both PTH and cAMP analogs
Martin et al., Endocrinology 1989 : Activation of PK-A in response to PTH is demonstrable at the lowest doses of PTH that alter phosphate transport
Shirakawa et al., Mol Cell Biol 1989 : In contrast, PKA and PKC did not activate NF-kappa B in nuclear extracts from unstimulated cells
Scheurich et al., J Exp Med 1989 : As activation of PKA does not slow down the degradation rate of TNF-Rs, but rather enhances protein synthesis dependent reexpression of TNF-Rs after transient PKC mediated transmodulation and after tryptic digestion of TNF-Rs, it is concluded that PKA stimulates TNF-R synthesis ... PKA mediated enhancement of TNF-R expression was predominantly observed in normal peripheral blood monocytes and tumor cell lines of myeloid origin ... As in these typical TNF producer cells, the production of TNF is also controlled by PKA and PKC, a regulatory circuit is proposed, by which these two independent signal pathways antagonistically regulate TNF production and, at the receptor level, TNF sensitivity
Takahashi et al., J Antibiot (Tokyo) 1989 : UCN-01 has been shown to inhibit PKC and protein kinase A (PKA) with IC50 values of 0.0041 and 0.042 microM, respectively, and UCN-02 has been shown to inhibit PKC and PKA with IC50 values of 0.062 and 0.25 microM, respectively
Hohmann et al., Int J Cancer 1989 (Cell Transformation, Neoplastic) : The effect of retinyl acetate ( RAC ) on the activity of cAMP dependent protein kinase (PKA) was studied in mouse 10T1/2 cells
Tamura et al., Biochem Biophys Res Commun 1989 : In contrast, PTH activation of cAMP dependent protein kinase (PKA) , while also rapid, was greater in magnitude ( 0.10 to 0.50 ), persistent, and occurred at a threshold level of 3 x 10 ( -10 ) M PTH, compared to 10 ( -8 ) M for PKC
Briseid et al., Acta Pharmacol Toxicol (Copenh) 1978 : It is suggested that the low PKA activity of the acetone activated enzyme preparation from plasma of rats treated with dextran was due to the loss of HMWK or a fraction of HMWK
Wattanapermpool et al., J Mol Cell Cardiol 1995 : Protein kinase A (PKA) dependent phosphorylation of C-protein and cardiac troponin I (cTnI) is known to be associated with a reduced sensitivity to Ca2+
Seldon et al., Mol Pharmacol 1995 : When PGE2 was used to activate adenylyl cyclase, however, rolipram potentiated cAMP accumulation, PKA activation, and inhibition of TNF-alpha generation
Jovov et al., J Biol Chem 1995 : Thus, functional CFTR is required for activation of the ORCC by PKA and ATP ... Moreover, the addition of hexokinase + glucose to the extracellular side prevented activation of the ORCCs by PKA and ATP in the presence of CFTR ... Moreover, the addition of hexokinase + glucose to the extracellular side prevented activation of the ORCCs by PKA and ATP in the presence of CFTR ... These experiments confirm that both the presence of CFTR as well as the presence of ATP on the extracellular side is required for activation of the ORCC by PKA and ATP
Kachra et al., Endocrinology 1994 : The synergistic augmentation of hepatocyte IGF-I mRNA levels by GH and glucagon involves the activation of PKA and PKC, but also appears to require the synthesis of one or more protein ( s )
Gwosdow et al., Am J Physiol 1994 : The present studies were conducted to determine if cAMP is involved in IL-1 alpha activation of PKA and if PKA is responsible for IL-1 alpha induced ACTH release from AtT-20 cells ... Inhibition of adenylate cyclase with 2'5'-dideoxyadenosine ( 2'5'-DDA ) did not affect IL-1 alpha induced increases in PKA activity and ACTH secretion ... Inhibition of adenylate cyclase with 2'5'-dideoxyadenosine ( 2'5'-DDA ) did not affect IL-1 alpha induced increases in PKA activity and ACTH secretion ... In contrast, CRF stimulated PKA activity and ACTH secretion were inhibited by 2'5'-DDA ... IBMX did not alter IL-1 alpha induced PKA activity or ACTH secretion, yet IBMX potentiated CRF induced cAMP accumulation ... Inhibition of PKA with the PKA inhibitor, H-8, blocked activation of PKA and ACTH secretion by both IL-1 alpha and CRF in AtT-20 cells ... Inhibition of PKA with the PKA inhibitor, H-8, blocked activation of PKA and ACTH secretion by both IL-1 alpha and CRF in AtT-20 cells ... These observations demonstrate that 1 ) the mechanism of IL-1 alpha activation of PKA is independent of adenylate cyclase or cAMP and 2 ) PKA is used by IL-1 alpha to induce ACTH secretion from AtT-20 cells
Tan et al., J Neurosci 1994 : In this study 32P labeling of non-NMDA GluRs was investigated in cultured hippocampal neurons stimulated 2-15 min with agonists that selectively stimulate either Ca2+/calmodulin dependent protein kinase II ( CaM-kinase II ), Ca2+/phospholipid dependent protein kinase C ( PKC ), or cAMP dependent protein kinase A (PKA) ... In this study 32P labeling of non-NMDA GluRs was investigated in cultured hippocampal neurons stimulated 2-15 min with agonists that selectively stimulate either Ca2+/calmodulin dependent protein kinase II ( CaM-kinase II ), Ca2+/phospholipid dependent protein kinase C ( PKC ), or cAMP dependent protein kinase A (PKA)
Abu-Lawi et al., Infect Immun 1995 : Inhibitors of PKC, PKA , and PKG did not block the phosphorylation of p14
Hirata et al., Hypertension 1995 : Moreover, we measured NO release from cultured BAECs by a chemiluminescence method to examine the effect of PKC and PKA on endothelial NOS activity
McDonald et al., Proc Natl Acad Sci U S A 1995 (Cystic Fibrosis) : Inhibition of PKA activity by expression of a dominant negative regulatory subunit or treatment with the PKA-selective inhibitor N- [ 2- ( p-bromocinnamylamino ) ethyl ] -5-isoquinolinesulfonamide ( H-89 ) caused a complete suppression of CFTR gene expression without affecting other constitutively active genes
Kennedy et al., Biochim Biophys Acta 1995 : Regulation of phospholipases C (PLC) and arachidonic acid ( AA ) release by cAMP dependent protein kinase (PKA) was investigated in MDCK-D1 cells ... The results strongly suggest a role for PKA in the regulation of PLC activity and AA release in MDCK-D1 cells
Moe et al., J Clin Invest 1995 : The present studies examined regulation of NHE-3 by PKA ... PKA activation ( 0.1 mM 8-BrcAMP x 20 min ) inhibited NHE-3 activity by 39 % ( P < 0.01 ) with no change in NHE-3 protein abundance in the plasma membrane ... In summary, acute activation of PKA inhibits NHE-3 activity, an effect that is likely mediated by phosphorylation of its cytoplasmic domain
Wong et al., J Clin Invest 1995 : When compared with basal MAPK activity of 26.4 +/- 6.6 pmol/mg/min in controls, basal MAPK activity varied widely in Ras transfected clones from 29.0 +/- 6.6 to 96.6 +/- 13.4 pmol/mg/min. Clones that functionally expressed activated Ras displayed complete abolition of AVP stimulated PKA activity, whereas those that failed to express elevated basal MAPK activity showed intact AVP stimulated PKA ... The correlation between expression of high basal MAPK activity and inhibition of AVP induced PKA yielded a correlation coefficient of -0.92 ( P = 0.009 ) ... The Raf clones showed no impairment of AVP stimulated PKA activity ... We conclude that expression of activated Ras is inhibitory of AVP induced PKA activation in the M-1 cortical collecting duct cell line at a site proximal to G alpha s protein
Wong et al., Neuroendocrinology 1995 : Stimulation of cytosolic PKA activity in rat somatotrophs by the adenylate cyclase activator forskolin ( 10 nM-1 microM ) and membrane permeant cAMP analog db.cAMP ( 5 microM-0.5 mM ) mimicked the GH-releasing effect of GRF ... Somatostatin ( SRIF) (1 nM ), the physiological GH-release inhibitor, suppressed the GH response to GRF without affecting the basal or GRF stimulated PKA activity ... In this case, SRIF also induced a small but significant inhibition of GRF stimulated PKA activity
Bernardo et al., J Membr Biol 1995 : PKA also inhibits the activity of the Na+/HCO3- cotransporter ... In the presence of trypsin, the inhibitory effect of CSU of cAMP-PKA on the activity of Na+/HCO3- cotransporter was blunted ... The effect of CSU of cAMP-PKA on the Na+/HCO3- cotransporter activity was assayed in proteoliposomes digested with trypsin with the addition of a fraction containing the 42 kDa protein ( fraction S+ ) or without the 42 kDa protein ( fraction S- ). ( ABSTRACT TRUNCATED AT 250 WORDS )
Costa Rosa et al., Biochem J 1995 : Regulation of glucose-6-phosphate dehydrogenase activity by PKA has not been reported elsewhere
Azizi et al., Eur J Endocrinol 1995 : We conclude that : the effect of isoproterenol on ANP release is sensitive to indomethacin and H89 ; PGE2, but not PGE2 alpha or PGI2 , increases ANP release ; isoproterenol promotes myocardial PGE2 formation independently of adenylate cyclase and PKA activation pathways ; and PGE2 induced ANP release is mediated by cAMP production and subsequent PKA activation ... We conclude that : the effect of isoproterenol on ANP release is sensitive to indomethacin and H89 ; PGE2, but not PGE2 alpha or PGI2, increases ANP release ; isoproterenol promotes myocardial PGE2 formation independently of adenylate cyclase and PKA activation pathways ; and PGE2 induced ANP release is mediated by cAMP production and subsequent PKA activation
Mitsuyama et al., Mol Cell Biochem 1995 : These observations suggest that not only Ca2+ influx but the inhibition of PKA is necessary for the maximum O2- production by fMLP and that the O2- production is partially suppressed by the activation of PKA induced by fMLP
Rajnarayan et al., Mol Endocrinol 1995 : To directly determine whether GHF-1 is necessary and sufficient to mediate the PKA activation of the rPRL promoter, we established a cotransfection reconstitution assay whereby the activity of an intact and site-specific mutants of the ( -425 to +73 ) rPRL promoter-luciferase reporter gene was reconstituted by cotransfecting expression vectors encoding for either the PKA beta catalytic subunit, GHF-1, or both, into HeLa nonpituitary cells
Lei et al., Mol Cell Endocrinol 1995 : Treatment with the PKA inhibitor alone modestly decreased GnRH mRNA levels suggesting that PKA signaling also controls the basal expression of the GnRH gene
Suwanichkul et al., J Biol Chem 1993 (Carcinoma, Hepatocellular...) : In addition, cotransfection of the catalytic subunit of cAMP dependent protein kinase A (PKA) with the native 1205-bp IGFBP-1 promoter construct stimulated IGFBP-1 promoter activity 3.9-fold, but the TAGCA mutation decreased by 73 % the ability of PKA to stimulate IGFBP-1 promoter activity above control levels
Graves et al., Proc Natl Acad Sci U S A 1993 : PKA did not inhibit MAPKK and MAPK activity in vitro, and MAPKK and MAPK from extracts of forskolin treated cells could be activated normally with purified Raf-1 and MAPKK, respectively, suggesting that PKA blocked signaling upstream of MAPKK
Hoesche et al., Biochim Biophys Acta 1995 (Neuroblastoma) : Transient expression of a CAT fusion gene under the control of the synapsin I promoter region is also inhibited by forskolin/IBMX, as well as by protein kinase A (PKA) overexpression, suggesting that the decrease of synapsin I mRNA in response to forskolin/IBMX is due to the inhibition of transcription
Volonté et al., J Neurosci Res 1995 : C-PKA and PKN are widely expressed enzymes and the possibility of PKN dependent modulation of PKA in intact cells would therefore have biological implications for signal transduction mechanisms
Raymond et al., Biochemistry 1994 : PKA activators did not induce a measurable desensitization of 5-HT1A receptor inhibited adenylyl cyclase activity
Neumann et al., EMBO J 1995 : In CD4+ T helper cells, stimulation of PKA leads to suppression of interleukin 2 (IL-2) induction, while induction of the genes coding for the lymphokines IL-4 and IL-5 is enhanced ... We show that the differential effect of PKA activity on induction of the IL-2 and IL-4 genes is mediated through their promoters ... We show that the differential effect of PKA activity on induction of the IL-2 and IL-4 genes is mediated through their promoters ... Mutations preventing factor binding to the IL-2 kappa B site result in a loss of PKA mediated suppression of IL-2 promoter activity ... Stimulation of the PKA pathway in Jurkat T cells with the PKA activator forskolin leads to an increase in synthesis of c-Rel and p105/p50 , while synthesis of p65/RelA remains unchanged ... Stimulation of the PKA pathway in Jurkat T cells with the PKA activator forskolin leads to an increase in synthesis of c-Rel and p105/p50, while synthesis of p65/RelA remains unchanged ... These results indicate that PKA mediated suppression of NF-kappa B activity plays an important role in the control of activation of peripheral T lymphocytes
Lok et al., FEBS Lett 1995 (Leukemia, Promyelocytic, Acute) : These results suggest that ( i ) the down-regulation of TfR mRNA expression during the differentiation of HL-60 cells can be mediated by activation of either PKC or PKA ; ( ii ) the constitutive expression of TfR mRNA in proliferating HL-60 cells is staurosporine-sensitive and is probably maintained by protein kinase ( s ) other than PKC and PKA
Murphy et al., Biochem Biophys Res Commun 1994 : These results imply that H-ras functions, in this system, to decrease levels of cAMP, thus negating the regulatory effect of PKA on PLC
Peraldi et al., FEBS Lett 1995 : Hence, these data indicate that the PKA mediated phosphorylation of B-Raf hampers its interaction with p21ras, which is responsible for the PKA mediated decrease in B-Raf activity
Slobodyansky et al., Am J Physiol 1995 : In the brain, dopamine, via protein kinase A (PKA) activation of dopamine- and cAMP regulated phosphoprotein ( DARPP-32 ), inhibits protein phosphatase 1 (PP1) activity and keeps Na ( + ) -K ( + ) -adenosinetriphosphatase (ATPase) in its phosphorylated inactive state
Li et al., Cell 1995 (Congenital Abnormalities) : Reduced protein kinase A (PKA) activity in anterior imaginal disc cells leads to cell-autonomous induction of decapentaplegic ( dpp ), wingless ( wg ), and patched (ptc) transcription that is independent of hedgehog ( hh ) gene activity
Singer-Lahat et al., Receptors Channels 1994 : Enhancement of cardiac L-type Ca2+ channel activity by norepinephrine via phosphorylation by protein kinase A (PKA) underlines the positive inotropic effect of this transmitter and is a classical example of an ion channel modulation
Gross et al., Gastroenterology 1995 : The aim of this study was to improve the understanding of the regulation of IL-8 synthesis by investigating the roles of protein kinase C ( PKC ), protein kinase A (PKA) , and protein tyrosine kinase ( PTK ) in the induction of IL-8
Warnock et al., J Cell Physiol 1995 (Second Messenger Systems) : The addition of various PKC inhibitors also blocked the IL-1 beta induction of PGHS-2 mRNA but did not alter PGHS-1 mRNA expression ; inhibitors of protein kinase A (PKA) or tyrosine kinase ( TK ) had only a limited effect on IL-1 beta induced PGHS-2 mRNA expression
Aguila-Mansilla et al., J Pharmacol Exp Ther 1993 : Localization of a defect in hypothalamic dopaminergic neurons of the aged brain that results in impaired PKA dependent activation of tyrosine hydroxylase
Kwok et al., Nature 1994 : CREB is activated through phosphorylation by protein kinase A (PKA) , but precisely how phosphorylation stimulates CREB function is unknown
Kim et al., J Neurochem 1994 (Neuroblastoma) : To define the precise role of cyclic AMP ( cAMP ) -dependent protein kinase (PKA) in transcriptional regulation of the tyrosine hydroxylase (TH) gene, we performed transient cotransfection analyses of a reporter construct containing the upstream 2,400 bp sequence of the rat TH gene with expression plasmids encoding a heat-stable specific inhibitor of PKA ( PKI ), a mutant regulatory subunit of PKA, or the catalytic subunit of PKA
Imaki et al., Brain Res 1994 : Cyclic AMP ( cAMP ) mediates the hormonal stimulation of a number of eukaryotic genes by directing the protein kinase A (PK-A) dependent phosphorylation of the transcription factor CREB
VanRenterghem et al., J Biol Chem 1994 : Furthermore, inhibition of endogenous PKA by the heat-stable PKA inhibitor is sufficient to stimulate MAPK activity in these extracts in the absence of protein synthesis and without dependence on a functional Ras protein
Krajewski et al., Mol Cell Biol 1994 : Remarkably, monomeric CREB acts as a potent, constitutive activator under conditions in which native CREB is inducible by PKA
Grieco et al., Proc Natl Acad Sci U S A 1994 : PKA does not block the accumulation of cyclin or its binding to p34cdc2 , but the resultant complex lacks kinase activity and p34cdc2 remains tyrosine phosphorylated ... PKA does not block the accumulation of cyclin or its binding to p34cdc2, but the resultant complex lacks kinase activity and p34cdc2 remains tyrosine phosphorylated
Hawes et al., J Biol Chem 1994 : Thus, the binding of G beta gamma to phosducin can be regulated by PKA-phosphorylation in an intact cell model system
Doolan et al., Br J Pharmacol 1994 : 4. Cyclic AMP stimulated PKA activity was inhibited by 10 microM AA and this effect was significantly enhanced by nordihydroguaiaretic acid ( NDGA ) + indomethacin (Indo) , inhibitors of the lipoxygenase and cyclo-oxygenase pathways of AA metabolism respectively ... 5. Oleic acid, elaidic acid, but not caprylic or palmitic acids, also significantly inhibited PKA activity and this effect was again enhanced by NDGA + Indo
Arias et al., Nature 1994 : Cyclic AMP regulates the expression of numerous genes, for example, through the protein kinase-A (PKA) mediated phosphorylation of transcription factor CREB at Ser 133
Raymond et al., Nature 1993 : Here we report that the GluR6 glutamate receptor, transiently expressed in mammalian cells, is directly phosphorylated by PKA, and that intracellularly applied PKA increases the amplitude of the glutamate response
Manié et al., J Biol Chem 1993 : The use of different protein kinase inhibitors supported a role of the PKA , but not the PKC, in the colchicine induced IL-1 production
Marti et al., Oncogene 1994 : Comparing nuclear extracts from normal mammary glands with nuclear extracts from glands which had been cleared of all epithelial cells 3 weeks after birth, revealed that PKA activation, AP-1 induction and Oct-1 inactivation all are dependent on the presence of the epithelial compartment ... The increased Fos/Jun expression and the inactivation of Oct-1 may be consequences of the increased PKA activity ... The increased Fos/Jun expression and the inactivation of Oct-1 may be consequences of the increased PKA activity
Quinn et al., J Biol Chem 1994 (Liver Neoplasms, Experimental) : The minimal promoter/transcription factor requirements for induction of phosphoenolpyruvate carboxykinase ( PEPCK ) transcription by cAMP activated protein kinase A (PKA) and inhibition of this induction by insulin were investigated
de Wit et al., Br J Haematol 1994 : Using the human monocytic cell line Mono Mac 6 we studied the involvement of Ca2+, protein kinase A (PKA) , and protein kinase C ( PKC ) dependent pathways in the regulation of M-CSF and IL-6 gene expression ... Using the human monocytic cell line Mono Mac 6 we studied the involvement of Ca2+, protein kinase A (PKA) , and protein kinase C ( PKC ) dependent pathways in the regulation of M-CSF and IL-6 gene expression
Robertson et al., Dementia 1993 (Alzheimer Disease) : Although the phosphorylated forms of tau generated by PKA are not identical to those of PHF-tau, PKA may be involved in the generation of PHF-tau in Alzheimer 's disease via phosphorylation of additional, as yet unidentified, sites on tau
Wieprecht et al., Biochem J 1994 : This inhibitory effect could be partly prevented by simultaneous addition of 10 microM forskolin, indicating that choline kinase is regulated in part by PKA activity
Bakhiet et al., Eur J Immunol 1993 : Although both the PKC and the PKA inhibitors suppressed PHA induced IFN-gamma secretion and proliferation of rat MNC and mouse CD8+ CD4- MNC, they had no effect on the same TLTF induced responses
Aksamit et al., J Immunol 1993 : In addition, since IL-1 and PGE2 can activate protein kinase C ( PKC ) and protein kinase A (PKA) , we exposed human lung fibroblasts to 1- ( 5-Isoquinoline sulfonyl ) -2-methylpiperafine ( H7 ) or staurosporine, which are relatively specific inhibitors of PKC, and N- [ 2- ( methylamino ) ethyl ] -5-isoquinolinesulfomamide ( H8 ), an inhibitor of PKA, to determine whether the IL-1 and PGE2 stimulated increases in binding sites were mediated by activation of PKC or PKA
Hagiwara et al., Mol Cell Biol 1993 : Cyclic AMP ( cAMP ) regulates a number of eukaryotic genes by mediating the protein kinase A (PKA) dependent phosphorylation of the CREB transcription factor at Ser-133
Palkama et al., Hum Immunol 1993 : The effect of PKC, PKA , and tyrosine kinase inhibitors on HLA-DR mediated IL-1 beta mRNA expression was then determined
Janulis et al., Endocrinology 1993 : Rat PTH- ( 1-34 ) ( 10 ( -7 ) M ) increased PKA activity 4.5-fold, but other fragments failed to stimulate PKA between 10 ( -12 ) -10 ( -6 ) M. Human PTH- ( 28-34 ) stimulation of PKC was variable from experiment to experiment ... The adenylate cyclase inhibitor 2',5'-dideoxyadenosine ( 10 ( -4 ) M ) reduced PTH- ( 1-34 ) -stimulated PKA activity by 60 %, but failed to block the rise in 1,25- ( OH ) 2D3 secretion
Gwosdow et al., Endocrinology 1993 (Pituitary Neoplasms) : Because IL-1 did not affect cAMP accumulation, the effect of IL-1 on protein kinase A (PKA) was investigated
Fukayama et al., Am J Physiol 1993 : We have used wild-type and adenosine 3',5'-cyclic monophosphate ( cAMP ) -resistant mutant osteoblast-like SaOS-2 cells to investigate the role of protein kinase A (PKA) in the regulation of cytosolic free Ca2+ concentration ([Ca2+]i)
Kessels et al., Biochem J 1993 : Taken together, our results indicate that in human neutrophils : ( i ) PKC activity is not essential for fMLP induced activation of PLD; (ii) PKC activity does play an essential role in the activation of the respiratory burst by fMLP, other than mediating or modulating PLD activation ; ( iii ) there exists a negative-feedback mechanism on fMLP induced PLD activation by concomitant activation of PKA
Prat et al., Am J Physiol 1993 : The cAMP dependent protein kinase A (PKA) in the presence of ATP induced and/or enhanced Na+ channel activity in excised inside-out patches with a change in average apparent channel number and percent open probability similar to those observed with either AVP or cAMP analogues in intact cells
Hajnóczky et al., Biochem J 1993 : However, the amount of compartmentalized fura-2 that could be quenched by Mn2+ in the presence of maximal Ins ( 1,4,5 ) P3 was increased by PKA
Muroi et al., Cell Signal 1993 : LPS induced activation of NF-kappa B was inhibited by a protein kinase A (PKA) inhibitor ( H-89 ), but not by a protein kinase C ( PKC ) inhibitor ( H-7 ) ... Thus, these data suggested a probable involvement of PKA in LPS induced NF-kappa B activation in macrophages
Grüne et al., J Biol Chem 1993 : Bt2cAMP induced increases in TC uptake and [ Ca2+ ] i were inhibited by known inhibitors of PKA and by an activator of PKC, but they remained unaffected by a specific inhibitor of PKC
Menez et al., Alcohol Alcohol 1993 : Phosphorylation of pure cytochrome P4502E1 (CYP2E1) was achieved in vitro using Ca2+/calmodulin dependent protein kinase II ( CaM kinase II ), protein kinase C ( PKC ) and cAMP dependent protein kinase (PKA) ... Phosphorylation of pure cytochrome P4502E1 (CYP2E1) was achieved in vitro using Ca2+/calmodulin dependent protein kinase II ( CaM kinase II ), protein kinase C ( PKC ) and cAMP dependent protein kinase (PKA)
Weinman et al., J Clin Invest 1993 : When assayed after reconstitution, PKA in the presence of ATP and Mg2+ did not inhibit Na ( + ) -H+ exchange transport in a fraction of solubilized BBM proteins eluting from an anion exchange column between 0.2 and 0.4 M NaCl ( fraction B )
Braha et al., J Neurosci 1993 : We then use specific blockers of protein kinase C ( PKC ) and the cAMP dependent protein kinase A (PKA) to examine the roles of PKC and PKA in mediating the effect of 5-HT on the nifedipine-sensitive Ca2+ current
Ishibashi et al., Biochem Biophys Res Commun 1993 : The result suggests that BMP7 gene expression may be regulated in MDCK cells by PKA and PKC
Caelles et al., Mol Cell Biol 1995 : As reported here, cAMP agonists and PKA do not inhibit GHF-1 activity in living cells and although they stimulate the phosphorylation of GHF-1, the inhibitory phosphoacceptor site within the homeodomain is not affected
Satoh et al., Hypertens Res 1995 : We reported a novel intracellular mechanism of renal Na-K-ATPase regulation by dopamine ( DA ) in the rat cortical collecting duct (CCD) , which involves stimulation of protein kinase A (PKA) and phospholipase A2 (PLA2)
Laxminarayana et al., J Immunol 1996 (Leukemia-Lymphoma, Adult T-Cell) : To confirm that PKA-I activation via the TCR/CD3 complex and IL-1R requires antecedent protein tyrosine kinase catalyzed phosphorylation of phospholipase C-gamma 1, we used wild-type and CD45-deficient ( mutant J45.01 ) Jurkat T cell lines ... To confirm that PKA-I activation via the TCR/CD3 complex and IL-1R requires antecedent protein tyrosine kinase catalyzed phosphorylation of phospholipase C-gamma 1, we used wild-type and CD45-deficient ( mutant J45.01 ) Jurkat T cell lines ... To confirm that PKA-I activation via the TCR/CD3 complex and IL-1R requires antecedent protein tyrosine kinase catalyzed phosphorylation of phospholipase C-gamma 1, we used wild-type and CD45-deficient ( mutant J45.01 ) Jurkat T cell lines
Graves et al., J Biol Chem 1996 : In summary, these results demonstrated that PDGF increases cAMP formation and PKA activity through a MAP kinase mediated activation of cPLA2 , arachidonic acid release, and PGE2 synthesis in human arterial smooth muscle cells
Post et al., J Biol Chem 1996 (Lymphoma) : This loss of sites in WT cells displayed a t1/2 of approximately 7 min, was agonist concentration dependent ( EC50 approximately 60 nM ), was not mimicked by 8-Br-cAMP, and could be blocked by the PKA inhibitor, H89 ... This loss of sites in WT cells displayed a t1/2 of approximately 7 min, was agonist concentration dependent ( EC50 approximately 60 nM ), was not mimicked by 8-Br-cAMP, and could be blocked by the PKA inhibitor, H89
Joseph et al., Am J Physiol 1995 : Because IL-1 alpha did not affect cAMP accumulation, the effect of IL-1 alpha on PKA activity was investigated ... IL-1 alpha increased ( P < 0.05 ) PKA activity at 15 and 30 min and returned to control levels by 1 h. Forskolin also increased ( P < 0.05 ) PKA activity ... These observations demonstrate that 1 ) IL-1 alpha stimulated dopamine release from PC-12 cells by activating PKA, 2 ) the mechanism of IL-1 alpha activation of PKA does not involve detectable increases in intracellular cAMP accumulation, and 3 ) IL-1 alpha activates type II PKA, which is used by IL-1 alpha to stimulate dopamine secretion from PC-12 cells
Ismailov et al., J Biol Chem 1996 (Ion Channel Gating) : Protein kinase A (PKA) plus ATP activated CFTR , but did not have any effect on rENaC
Xie et al., Cell Immunol 1996 (Lymphoma, B-Cell) : By using specific inhibitors for protein kinase C ( PKC ), Ca2+/calmodulin dependent protein kinase II ( CaM kinase II ), and protein kinase A (PKA), we found that anti-Ig induced CREB Ser133 phosphorylation depends on PKC, but does not require activation of PKA or CaM kinase II
Yang et al., Mol Cell Biol 1996 : We investigated the effect of cyclic AMP dependent protein kinase (PKA ) on v-Mos kinase activity ... Increase in PKA activity in vivo brought about either by forskolin treatment or by overexpression of PKA catalytic subunit resulted in a significant inhibition of v-Mos kinase activity
Crestani et al., J Lipid Res 1995 (Second Messenger Systems) : Finally, cyclic AMP and protein kinase A (PKA) stimulated the expression of the CYP7/luciferase gene through multiple sequences in the distal and proximal regions, and both positive and negative response regions were mapped
Richards et al., J Biol Chem 1996 : The transcription factor CREB ( cAMP responsive element binding protein ) is activated by protein kinase A (PKA) phosphorylation of a single serine residue ... Protease cleavage patterns indicate that PKA phosphorylation does not induce a global conformational change in CREB ... Since PKA phosphorylation of CREB results in its specific binding to the transcriptional co-activators CREB binding protein and p300, we suggest that the PKA activation of CREB occurs by the production of specific, complementary interactions with these proteins, rather than through the previously proposed mechanisms of a phosphorylation dependent conformational change or increased DNA binding affinity
Berdiev et al., J Biol Chem 1996 (Ion Channel Gating) : 4 ) DNase I , an agent known to prohibit elongation of actin filaments, prevented activation of alpha, beta, gamma-rENaC by ATP or PKA plus ATP
Itano et al., J Neurochem 1996 (Neuroblastoma) : From these results, we suggest that the expression of Bcl-2 proteins is regulated by PKC and PKA in positive and negative manners, respectively, in SH-SY5Y cells
Yu et al., J Biol Chem 1996 : The fenoldopam induced increase in PLC-gamma and activity was mediated by protein kinase A (PKA) since it was blocked by the PKA antagonist Rp-8-CTP-adenosine cyclic 3 ' : 5'-monophosphorothioate ( Rp-8-CTP-cAMP-S ) and mimicked by direct stimulation of adenylyl cyclase with forskolin or by a PKA agonist, Sp-cAMP-S ... We suggest that the D1A mediated stimulation of PLC occurs as a result of PKA activation ... PKA then stimulates PLC-gamma in cytosol and membrane via activation of PKC
Ju et al., Glycoconj J 1995 : The activities of GnTV and membranous protein kinase A (PKA) were also induced in parallel by dibutyryl cAMP ( db-cAMP ) and this induction was blocked by a specific PKA inhibitor
Cashman et al., Mol Chem Neuropathol 1995 (Alzheimer Disease) : The purpose of this study was to compare the effects of aging and Alzheimer disease (AD) on the important intracellular signaling enzyme cAMP dependent protein kinase A (PKA) in cerebral microvessels
Tsai et al., Endocrinology 1996 : PGHS-2 was acutely ( < 12 h ) increased by PKA , and to a lesser extent by PKC ... Temporal expression of FP receptor mRNA is not consistent with the involvement of FP receptor in ovulation and suggests that PKA stimulates PGHS-2 and FP receptor mRNA by distinct mechanisms
Yang et al., Pflugers Arch 1996 : These results suggest that activation of cAMP/PKA might inhibit the 5-HT stimulated phosphoinositide breakdown and consequently reduce the [Ca2+ ] i increase or inhibit both responses independently
Montminy et al., Metabolism 1996 : The kinetics of protein kinase (PK-A) dependent cAMP response element binding protein ( CREB ) phosphorylation closely parallel the changes in transcription of cAMP-responsive genes by run-on assay
Mischak et al., Mol Cell Biol 1996 : As the binding to GTP loaded Ras induces Raf-1 activation in the cell, we examined which mechanism is required for PKA mediated Raf-1 inhibition in vivo
Bengis-Garber et al., Cell Signal 1996 : These results indicated that the PKA dependent downregulation of fMLP induced p47 phox phosphorylation apparently involves step ( s ) in the fMLP signaling pathway that are upstream of PKC
Lin et al., Pathobiology 1995 : Anti-CD2 also increased the activity of cAMP dependent protein kinase (PKA)
Yokoo et al., Kidney Int 1996 : Unexpectedly, the inhibitory action of PKA was not through cAMP response element ( CRE ) but through TRE, because ( i ) activation of CRE was not induced by IL-1 beta, and ( ii ) cAMP mediated activation of PKA suppressed the basal TRE activity ... Unexpectedly, the inhibitory action of PKA was not through cAMP response element ( CRE ) but through TRE, because ( i ) activation of CRE was not induced by IL-1 beta , and ( ii ) cAMP mediated activation of PKA suppressed the basal TRE activity
Ree et al., Clin Exp Metastasis 1996 (Breast Neoplasms) : These data suggest that activation of PKC but not of PKA may induce transcription of MMP-1 and TIMP-1 , possibly by the synthesis of transcription factor ( s ), in transformed cells of epithelial origin ... These data suggest that activation of PKC but not of PKA may induce transcription of MMP-1 and TIMP-1, possibly by the synthesis of transcription factor ( s ), in transformed cells of epithelial origin
Igual et al., Mol Gen Genet 1996 : We have also analyzed the role of the cAMP dependent protein kinase (PKA) in the transcriptional regulation of POT1
Henry et al., J Physiol 1996 : PKA activators caused no change in KIR1.1 , KIR2.1, or KIR2.3 current
Melamed et al., Neuroendocrinology 1996 (Second Messenger Systems) : The involvement of PKA and PKC in the GnRH stimulatory effect on both GtH release and GtH II beta mRNA levels was corroborated by use of the PKA and PKC inhibitors, H89 and GF109203X, respectively ( 100 nM ) which attenuated the GnRH effect
Ye et al., EMBO J 1996 : The binding of WT1ZF - KTS to an EGR1 consensus binding site was also inhibited by prior PKA and PKC phosphorylation ... The binding of WT1ZF - KTS to an EGR1 consensus binding site was also inhibited by prior PKA and PKC phosphorylation
Skouteris et al., Biosci Rep 1996 : Therefore it is suggested that PKA mediated at a great extent the co-stimulatory effects of PTH on hepatocyte proliferation via IP3 production
Romano et al., Brain Res 1996 (Amnesia) : Activation of crab PKA by Sp-5,6-DCl-cBIMPS and its inhibition by Rp-8-Cl-cAMPS were assessed using an in vitro PKA activity assay
Marambaud et al., J Neurochem 1996 : Altogether, this report demonstrates that the alpha-secretase pathway is under the control of PKA in human cells and that the proteasome likely contributes, either directly or through yet unknown intermediates, to the PKA stimulated APP alpha secretion in human cells
Enan et al., Reprod Toxicol 1996 : The stimulatory effect of TCDD on postnuclear PKA activity was enhanced by insulin and TCDD reversed the negative effect of FK, but there was no effect of either insulin or FK on the inhibition by TCDD of nuclear PKA activity
Sugiyama et al., J Immunol 1997 : The role of cAMP dependent protein kinase (PKA) in the regulation of TCR triggered IL-2 secretion was studied by transfecting T hybridoma cells with cDNA encoding the inhibitory regulatory subunit ( RI alpha ) of PKA with mutations in cAMP binding sites ( RI alpha ( m ) ) or by pretreating T cells with catalytic subunit-alpha ( C alpha ) antisense mRNA oligonucleotides
Lee et al., J Cell Physiol 1997 : We showed the following points : 1 ) in micromass cultures, activation of PKA enhances chondrogenesis and increases the phosphorylation of CREB; 2 ) BMP-2, a chondrogenic stimulator, increases PKA activity and the level of phosphorylated CREB ( P-CREB ) ; 3 ) H8, a PKA inhibitor, inhibits chondrogenesis ; 4 ) the chondrogenic activities of BMP-2 and cAMP are suppressed by H8 ; and 5 ) long-term TPA treatment ( a protein kinase C ( PKC ) modulator ) inhibits chondrogenesis and decreases the levels of CREB and P-CREB ... We showed the following points : 1 ) in micromass cultures, activation of PKA enhances chondrogenesis and increases the phosphorylation of CREB; 2 ) BMP-2, a chondrogenic stimulator, increases PKA activity and the level of phosphorylated CREB ( P-CREB ) ; 3 ) H8, a PKA inhibitor, inhibits chondrogenesis ; 4 ) the chondrogenic activities of BMP-2 and cAMP are suppressed by H8 ; and 5 ) long-term TPA treatment ( a protein kinase C ( PKC ) modulator ) inhibits chondrogenesis and decreases the levels of CREB and P-CREB ... We showed the following points : 1 ) in micromass cultures, activation of PKA enhances chondrogenesis and increases the phosphorylation of CREB; 2 ) BMP-2, a chondrogenic stimulator, increases PKA activity and the level of phosphorylated CREB ( P-CREB ) ; 3 ) H8, a PKA inhibitor, inhibits chondrogenesis ; 4 ) the chondrogenic activities of BMP-2 and cAMP are suppressed by H8 ; and 5 ) long-term TPA treatment ( a protein kinase C ( PKC ) modulator ) inhibits chondrogenesis and decreases the levels of CREB and P-CREB
Xiao et al., J Biol Chem 1997 : These PKA inhibitors had no effect on the PAH induction of the cyp1A1
Lund et al., Steroids 1997 : Furthermore it is not known how PKA regulates the activity of Pbx1 or its possible binding partners
Frohnwieser et al., J Physiol 1997 : From our data we conclude that the effects of PKA on hH1 are conferred by the large cytosolic loop interconnecting transmembrane domains I and II, which is not conserved among sodium channel subtypes
White et al., J Neurosci Res 1997 : In C alpha EV6 cells, activation of PKA by 80 microM ZnSO4 upregulated the expression of C alpha mRNA with maximal levels reached 8 hr post-treatment and maintained at 24 hr. Reporter gene assays in C alpha EV6 cells following transfection with increasing lengths of the NF-L promoter demonstrated that both a putative Sp1-like and a cAMP response ( CRE ), but not a NGFI-A, element were likely involved in PKA dependent activation of the NF-L promoter
Xu et al., J Mol Cell Cardiol 1997 : Activation of SR CaM kinase with Ca2+ and calmodulin, or induction of phospholamban phosphorylation by exogenous PKA , resulted in stimulation of the Ca2+ uptake activity of SR in fetal, newborn and adult heart
Wright et al., J Exp Biol 1997 : We conclude that PKA and PKC regulate rabbit SGLT1 activity by modulating the number of cotransporters in the plasma membrane and that this occurs through regulation of exocytosis and endocytosis
Houglum et al., J Clin Invest 1997 : Moreover, the phosphorylation of CREB on Ser133 was stimulated in activated cells by inducing the activity of PKA or CAMK-II
Corraliza et al., Biochim Biophys Acta 1997 : These results strongly suggest that PKA is involved in the induction of arginase and supports the hypothesis that there is a reciprocal regulation of these two enzymes that drives the macrophages towards opposite functional states
Onishi et al., Endocrinology 1997 (Osteosarcoma) : The inhibition of cell cycle progression through PKA mediated p27Kip1 induction might play an important role in PTH induced differentiation of osteoblasts
Luo et al., Cell Signal 1997 : These results suggest that activation of cyclic AMP/PKA might inhibit the BK-stimulated PI breakdown and consequently reduce the [ Ca2+ ] i increases or inhibit independently both responses, which is distal to the BK receptor in canine cultured TSMCs
Harris et al., Biochem Biophys Res Commun 1997 : We have now modified PKI sequences by N-terminal myristoylation to enhance their membrane permeability, and have used the myristoylated ( myr ) peptides to investigate the role of PKA activation in glucose induced insulin secretion from intact pancreatic beta-cells ... In experiments using intact islets, myr PKI14-22 caused a concentration dependent inhibition of insulin secretion in response to the PKA activators dibutyryl cyclic AMP and forskolin, suggesting that it gained access to the cytosolic compartment of intact beta-cells and inhibited PKA in situ ... However, these concentrations of myr PKI14-22 did not inhibit insulin secretion in response to glucose suggesting that the activation of PKA is not required for the initiation of glucose induced insulin secretion
Anciaux et al., J Neurosci Res 1997 (Glioma) : The membrane-permeable PKA inhibitor N- [ 2- ( p-bromocinnamylamino ) ethyl ] -5-isoquinoline sulfonamide ( H89 ) did not suppress the induction of GFAP-mRNA and its translation into GFAP ... Although all components of the PKA signal transduction pathway are present in C6, GFAP synthesis is not dependent on PKA activation but required the synthesis of an unidentified factor
Gekle et al., Am J Physiol 1997 : Activation of protein kinase A (PKA) by adenosine 3',5'-cyclic monophosphate, forskolin, or parathyroid hormone (PTH) reduced uptake to approximately 65 % of control
Brown et al., Arch Biochem Biophys 1997 : Moreover, PB induction of CYP3A23 and of CYP2B1/2 mRNAs was greatly diminished by inhibitors of cAMP dependent protein kinase (PKA)
Hong et al., Biochem Mol Biol Int 1997 : We suggest that PRL negatively regulates the PCNA-gene transcription by interfering with the cAMP/PKA mediated induction of CREB binding to the CRE-sequences and thereby suppresses DNA synthesis in regenerating rat liver ... We suggest that PRL negatively regulates the PCNA-gene transcription by interfering with the cAMP/PKA mediated induction of CREB binding to the CRE-sequences and thereby suppresses DNA synthesis in regenerating rat liver
Yan et al., J Biol Chem 1997 : A 17-amino acid synthetic peptide comprising this site completely inhibited the PKA dependent phosphorylation of TTF-1 in vitro ... Phosphorylation of TTF-1 mediates PKA dependent activation of surfactant protein B gene transcription
Beales et al., Cytokine 1997 : The aim of this study was to investigate the roles of protein kinase A (PKA) , protein kinase C ( PKC ), protein tyrosine kinase ( PTK ) and intracellular calcium in the induction of IL-8 production by gastric epithelial cells ... Stimulation of PKA with forskolin or dibutyryl cyclic adenosine monophosphate or inhibition with H89 had no effect on IL-8 production
Goldman et al., Recent Prog Horm Res 1997 (Second Messenger Systems) : In this review, we will discuss the mechanisms involved in the PKA dependent activation of CREB and describe how the co-activator CBP and its homologue are involved in this process
Bornfeldt et al., J Clin Invest 1997 : In these cells, PDGF induced MAPK activation leads to cytosolic phospholipase A2 activation, PGE2 release, and subsequent activation of the cAMP dependent protein kinase (PKA) , which acts as a strong inhibitor of SMC proliferation
Rohlff et al., J Biol Chem 1997 : Reporter gene activity under the control of the Sp1 dependent SV40 promoter is stimulated in insect cells transiently expressing Sp1 and PKA, and the DNA binding activity of recombinant Sp1 is activated by exogenous PKA in vitro
Lee et al., J Biol Chem 1997 : The PKC inhibitors also slowed the decay rate of the Ca2+ response induced by 300 microM ATP, and the PKA inhibitors increased it when induced by 30 microM ATP
Poleev et al., Eur J Biochem 1997 (Wilms Tumor) : Stimulation of cAMP synthesis augments Pax8-specific transcription in thyroid cells, indicating that PKA is involved in Pax8 activation
Ohlmeyer et al., Genes Dev 1997 : In addition, we find that Hh and Smo can stimulate target gene expression at constant Ci levels and that increased PKA activity can induce ectopic Hh target gene expression in a manner that requires Smo and Ci activities but does not involve changes in Ci protein concentration
Chen et al., Eur J Biochem 1997 (Brain Neoplasms) : In addition, PKA mediated transcriptional regulation of grp78 in OA -- > HS treatment is through regulation of protein phosphorylation as well as de novo synthesis of ATF-2
Cheng et al., Am J Physiol 1997 : PKA mediated phosphorylation and inhibition of Na ( + ) -K ( + ) -ATPase in response to beta-adrenergic hormone
Takuma et al., Biochem Mol Biol Int 1997 : Isoproterenol, forskolin, and CPS-cAMP markedly stimulated the phosphorylation of CREB in parotid acinar cells, and PKA inhibitors H-8 and H-89 dose-dependently inhibited it
Kamphuis et al., J Neuroimmunol 1997 : We could observe that PKA is involved in IL-4 mediated PPE mRNA induction, whereas IL-10 apparently uses another route
Ding et al., Cell Signal 1997 : Furthermore, elevation of cAMP may inhibit IP3 production and [ Ca2+ ] i mobilization through mechanisms involving PKA dependent phosphorylation of PLC , G-proteins, IP3 receptor and/or IP3 metabolizing enzymes
Fournier et al., J Biol Chem 1997 : Neither COX1 nor COX2 protein levels were affected by TNFalpha or PKA activators used alone, whereas in association, marked up-regulation of COX2 mRNA and protein was observed
Fishman et al., Cancer Res 1997 (Fibrosarcoma...) : However, based on the discovery that activated PKA may inhibit ras mediated translocation of c-Raf-1 to the plasma membrane, it is generally accepted that the cross-talk between cAMP/PKA and p21ras mediated signal transduction pathways is unilateral, i.e., that the activation of PKA regulates growth factor receptor protein tyrosine kinase mediated signaling ... However, based on the discovery that activated PKA may inhibit ras mediated translocation of c-Raf-1 to the plasma membrane, it is generally accepted that the cross-talk between cAMP/PKA and p21ras mediated signal transduction pathways is unilateral, i.e., that the activation of PKA regulates growth factor receptor protein tyrosine kinase mediated signaling ... Most importantly, our experiments have demonstrated the existence of a novel character of interactions between EGFR and PKA, because the ligation of the EGFR by epidermal growth factor in the metastatic variant induced a high activity of PKA
Ding et al., Biochem Biophys Res Commun 1997 : It is concluded that HP and its toxin can increase IL-8 expression in endothelial cells, and the expression of IL-8 elicited by HP toxin, TNF-alpha, and LPS is partially dependent on PTK but not PKA or PKC activation
Gradin et al., J Cell Biol 1998 : The results show that phosphorylation of Op18 on two specific sites, Ser-16 and Ser-63, is necessary and sufficient for PKA to switch off Op18 activity in intact cells
Borger et al., J Allergy Clin Immunol 1998 : We have recently shown that IL-4 gene expression can be inhibited by PKA activation but depends on the mode of T-cell activation
Cheng et al., Invest Ophthalmol Vis Sci 1998 : The inductions of VEGF and bFGF mRNA expression by PGE2 were blocked by the specific PKA inhibitors H-89 ( 30 microM ) or SQ 22536 ( 500 microM, 1000 microM )
Van der Kloot et al., J Physiol 1998 : ( In other preparations CGRP also activates PKA . )
Huang et al., Neuropharmacology 1997 : In conclusion, both PKA and PKC negatively regulate the activity of the GABA(A) receptor-channel complex probably through phosphorylation of the receptor, and the PKA system underlies the mechanism of mercury chloride potentiation of GABA activated currents
Bullock et al., Biochemistry 1998 : Fluorescent electrophoretic mobility shift assays show that phosphorylation by PKA results in a 3-5-fold increase in the binding affinity of CREB to both the symmetrical somatostatin CRE (SMS-CRE) and the asymmetric somatostatin upstream element (SMS-UE) ... Fluorescent electrophoretic mobility shift assays show that phosphorylation by PKA results in a 3-5-fold increase in the binding affinity of CREB to both the symmetrical somatostatin CRE (SMS-CRE) and the asymmetric somatostatin upstream element (SMS-UE) ... Processive phosphorylation of CREB by GSK-3 attenuates the enhanced DNA binding in response to PKA thus acts as an inhibitor of PKA induced binding
Yao et al., J Biol Chem 1998 : The cyclic adenosine monophosphate dependent protein kinase (PKA) is required for the sustained activation of mitogen activated kinases and gene expression by nerve growth factor ... Here we show that maximal sustained activation of ERK1 by NGF requires PKA ... Here we show that maximal sustained activation of ERK1 by NGF requires PKA ... Inhibitors of PKA partially blocked activation of ERK1 by NGF but had no effect on activation of ERK1 by EGF ... Inhibitors of PKA partially blocked activation of ERK1 by NGF but had no effect on activation of ERK1 by EGF ... Inhibition of PKA also reduced the ability of NGF and cAMP, but not EGF, to activate the transcription factor Elk-1 , reduced the induction of both immediate early and late genes after NGF treatment, and blocked the nuclear translocation of ERK1 induced by NGF ... Inhibition of PKA also reduced the ability of NGF and cAMP, but not EGF, to activate the transcription factor Elk-1, reduced the induction of both immediate early and late genes after NGF treatment, and blocked the nuclear translocation of ERK1 induced by NGF
Guyot et al., Immunol Lett 1998 : Phosphorylation was not associated with the induction of cAMP, and inhibition of PKA signaling had no effect on P-CREB
Mita et al., Comp Biochem Physiol C Pharmacol Toxicol Endocrinol 1996 : The PKA activity required Mg2+ at concentrations between 2 and 10 mM
Srivastava et al., Mol Cell Biol 1998 (Breast Neoplasms...) : In addition, paclitaxel- and vincristine induced activation of cyclic AMP ( cAMP ) -dependent protein kinase ( protein kinase A [PKA ] ) induced Bcl2 hyperphosphorylation and apoptosis, which were blocked by the PKA inhibitor Rp diastereomers of cAMP ( Rp-cAMP )
Tian et al., J Biol Chem 1998 : However, PKA mediated inhibition of BK channel activity in isolated patches from steroid treated cells was severely blunted
Fischer et al., Pflugers Arch 1998 : This suggests that, in CFTR expressing 3T3 cells, a phosphorylation site of CFTR is regulated by cellular PKA , PP2B and another phosphatase ... This suggests that, in CFTR expressing 3T3 cells, a phosphorylation site of CFTR is regulated by cellular PKA , PP2B and another phosphatase
Moroo et al., Brain Res 1998 : Neither an inhibitor of PKA ( H89 ) nor inhibitors of PKC ( staurosporine and calphostin C ) had any effect on the ERK2 activation induced by 1 pM PACAP38
Wang et al., Shi Yan Sheng Wu Xue Bao 1996 (Stomach Neoplasms) : [ Co-regulative effect of PKA-RII and PKC-alpha kinase subspecies on expression of c-myc and c-H-ras in human gastric cancer cells ( MGC 80-3 ) ]
Maizels et al., Endocrinology 1998 : FSH induced phosphorylation of p38 MAPK was blocked by pretreatment with the protein kinase A (PKA) inhibitor H89 and mimicked by the cAMP generating agonist forskolin, indicating that FSH induced cAMP production and PKA activation are necessary and sufficient for the activation of p38 MAPK in GC
Ghee et al., Brain Res Mol Brain Res 1998 (Teratocarcinoma) : In F9 and NIH3T3 cells, CBP, a co-activator which targets Fos-Jun and PKA stimulated CREB , also induced the TH promoter
Ortmeyer et al., J Basic Clin Physiol Pharmacol 1997 (Diabetes Mellitus, Type 2...) : Insulin decreases skeletal muscle cAMP dependent protein kinase (PKA) activity in normal monkeys and increases PKA activity in insulin-resistant rhesus monkeys
Sharma et al., Mol Cell Biochem 1998 : Phosphorylation of histone IIs was inhibited by W-7 ( calmodulin inhibitor ) and KN-62 ( CaM-kinase inhibitor ) with IC50 of 1.5 and 0.25 microm respectively, but was not affected by inhibitors of PKA ( Sigma P5015 ) and PKC ( H-7 )
Yubero et al., Mol Endocrinol 1998 : A double-point mutation in the -139/-122 element abolished both PKA- and c-Jun dependent regulation through this site, and overexpression of CREB blocked c-Jun repression
Kotani et al., Mol Cell 1998 : PKA and MPF activated polo-like kinase regulate anaphase promoting complex activity and mitosis progression ... These results indicate that Plk and PKA regulate mitosis progression by controlling APC activity
Zhong et al., Mol Cell 1998 : The transcriptional activity of NF-kappa B is stimulated upon phosphorylation of its p65 subunit on serine 276 by protein kinase A (PKA) ... Therefore, PKA regulates the transcriptional activity of NF-kappa B by modulating its interaction with CBP/p300 ... Therefore, PKA regulates the transcriptional activity of NF-kappa B by modulating its interaction with CBP/p300
Millet et al., J Bone Miner Res 1998 : Moreover, we show that PGE2 mediated IL-6 induction is prevented by the cAMP antagonist, Rp-cAMP, and the protein kinase A (PKA) inhibitors, KT5720 and H89
Agati et al., J Biol Chem 1998 : Thus, insulin inhibition of PKA induced PEPCK expression does not require MAPK activation but does require activation of PI3-kinase, although this signal is not transmitted through the PKB or PKC pathways ... Thus, insulin inhibition of PKA induced PEPCK expression does not require MAPK activation but does require activation of PI3-kinase , although this signal is not transmitted through the PKB or PKC pathways
Zidovetzki et al., AIDS Res Hum Retroviruses 1998 : This biological effect of Tat involved activation of both protein kinase C ( PK-C ) and cAMP dependent protein kinase (PK-A) in CNS-ECs ... Incubation of cells with the cyclooxygenase inhibitor indomethacin did not affect Tat induced activation of PK-A , indicating that prostacyclins are not involved in this process ... Tat induced increase in IL-6 mRNA was abolished in the presence on PK-A inhibitor H-89, demonstrating that activation of PK-A is necessary and sufficient for the increase in IL-6 production by these cells
Miras-Portugal et al., FEBS Lett 1998 : Activation of PKA or PKC, directly or through membrane receptors, results in a decrease of affinity or in reduction of the Ca2+ transient respectively
Rogue et al., Proc Natl Acad Sci U S A 1998 : Measurement of ATP dependent 45Ca2+ uptake into purified nuclei showed that PKA phosphorylation enhanced the Ca2+ pumping activity of NCA
Lorenz et al., Eur J Immunol 1998 : Based on the data presented, we propose that CD45 signaling in T lymphocytes subsequently activates cAMP accumulation and PKA activation in monocytes via LFA-1/ICAM-1 dependent cellular interactions
Takagi et al., Diabetes 1998 : A protein kinase A (PKA) inhibitor ( H89, 20 micromol/l ) suppressed hypoxia induced GLUT1 mRNA expression by 42 +/- 9 % ( P < 0.01 )
Deshpande et al., J Interferon Cytokine Res 1998 : In contrast, the myelomonocytic cell lines ( WEHI-3B, U-937 ) and peripheral blood neutrophils ( PMNC ) showed a rapid decrease in PKA activity in response to G-CSF ... H-89, a specific inhibitor of PKA, blocked G-CSF induced PKA activation in HL-60 cells but did not affect ligand induced downmodulation of G-CSF-R ... Indomethacin, an inhibitor of the cyclooxygenase pathway and prostaglandin synthesis, did not inhibit PKA induction in G-CSF treated HL-60 cells
Wang et al., Mol Pharmacol 1998 : This indicates that PKA plays a substantial role in angiotensin II-stimulated AT1-R mRNA down-regulation
Yurko-Mauro et al., Am J Physiol 1998 : Our data suggest that activation of PKC can enhance PKA dependent CFTR activation
Braiman et al., Am J Physiol 1998 : PKA induces Ca2+ release and enhances ciliary beat frequency in a Ca2+ dependent and -independent manner
Zeng et al., J Pharmacol Exp Ther 1998 : Pharmacological agents that increase intracellular concentration of cyclic AMP ( [ cAMP ] i ) mimicked and synergistically enhanced induction of IL-6 secretion by PGE2, whereas inhibitors of protein kinase A (PKA) but not protein kinase C suppressed PGE2 evoked increases in IL-6 secretion, suggesting that cAMP and PKA are the intracellular messengers of the PGE2 effect
Sakaguchi et al., Biochem Pharmacol 1998 : In contrast, the established PKA inhibitors H-8 and H-89 inhibited CaMK I with relatively high IC50 values of > 100 and 24.4+/-3.2 microM, respectively
Wyatt et al., Am J Physiol 1998 : cAMP levels and PKA activity are increased in BBECs stimulated with 0.01-1 mM isoproterenol, with a corresponding increase in CBF ... These studies suggest that the activation of either PKA or PKG can lead to the stimulation of CBF in bovine airway epithelium
Jaspers et al., J Cell Physiol 1998 (Adenocarcinoma, Bronchiolo-Alveolar...) : Exposure to ozone increased interleukin 8 expression and transcription factor activities in a protein tyrosine kinase ( PTK ) -dependent and protein kinase A (PKA) dependent , yet protein kinase C ( PKC ) -independent, manner ... Exposure to ozone increased interleukin 8 expression and transcription factor activities in a protein tyrosine kinase ( PTK ) -dependent and protein kinase A (PKA) dependent , yet protein kinase C ( PKC ) -independent, manner
Impey et al., Neuron 1998 (Calcium Signaling) : Interestingly, the Ca2+ induced nuclear translocation of ERK and Rsk2 to the nucleus requires protein kinase A (PKA) activation ... Interestingly, the Ca2+ induced nuclear translocation of ERK and Rsk2 to the nucleus requires protein kinase A (PKA) activation
Nishizaki et al., Brain Res 1998 : The present study was designed to assess the effect of protein kinase C ( PKC ) and cAMP dependent protein kinase (PKA) on desensitization of Torpedo acetylcholine (ACh) receptors by analyzing summated macroscopic currents in an outside-out patch-clamp configuration
Goretzki et al., Biochem J 1998 : Here, we investigated whether ligation of LRP increases the intracellular cAMP level and PKA activity via a stimulatory GTP binding protein ... Addition of the 39 kDa receptor associated protein (RAP), an antagonist for ligand interactions with LRP, blocked the lactoferrin induced increase in PKA activity, demonstrating a requirement for ligand binding to LRP
Sabatier et al., J Physiol 1998 : 4. The activation of protein kinase A (PKA) with Sp-cAMPS caused an increase in [Ca2+ ] i which was additive to the vasopressin elicited [Ca2+ ] i increase
Lévesque et al., Biochem Biophys Res Commun 1998 : We further established that the transition proteins are in vitro substrates for the Ca+2-phospholipid dependent protein kinase ( PKC ) as well as the cAMP dependent protein kinase (PKA)
Hu et al., J Biol Chem 1999 : These results indicate that the CRR binding activity and the Ras-suppressive function of Rap1A can be modulated through phosphorylation and suggest that Rap1A may function as a PKA dependent regulator of Raf-1 activation, not merely as a suppressor
Sakakibara et al., Neuroendocrinology 1998 : We hypothesize that activation of PDE4D3 by PKA may constitute a negative feedback signaling pathway which participates in the regulation of cAMP levels
Shin et al., Cytokine 1998 : Taken together, these results provide evidence that human CD8 ( + ) T cells are also the source of massive expression of IL-17, and that PKA plays a prominent role in the switch of CD8 ( + ) T cells to a Th2 like profile and an inhibition of IL-17 expression, thus suggesting that the activation of cAMP signal transduction pathway may have consequences for the relative role of CD8 ( + ) T cells in the immune and inflammatory process
Venkataraman et al., Mol Immunol 1998 : PKA-activation caused a downregulation of anti-IgM induced expression of Bcl-xL protein, that was restored by IL-4
LaVoie et al., Endocrinology 1999 : IGF-I significantly augmented PKA pathway agonist activation of the StAR promoter, whereas IGF-I had no effect alone
Chen et al., Mol Cell Biol 1999 : Heterodimer formation between ERalpha and ERbeta is similarly affected by PKA phosphorylation of serine 236 of ERalpha
Putz et al., Cancer Res 1999 (Prostatic Neoplasms) : We have studied the effects of EGF, IGF-I, and the protein kinase A (PKA) activator forskolin on the activation of p42/ extracellular signal regulated kinase ( ERK ) 2, which is a key kinase in mediation of growth factor induced mitogenesis in prostate cancer cells
Mulder et al., Diabetes 1999 : Because HSL in adipocytes is activated by cAMP dependent protein kinase (PKA) , PKA regulated triglyceride hydrolysis in beta-cells may participate in the regulation of insulin secretion, possibly by providing a lipid derived signal, e.g., long-chain acyl-CoA and diacylglycerol
Massheimer et al., Calcif Tissue Int 1999 : When the aged rats were injected with 25 ( OH ) D3, similar Ca2+ influx, cAMP, and PKA responses to in vitro stimulation with calcitriol were obtained
Zhao et al., J Biol Chem 1999 : A dominant negative PKA-regulatory subunit completely abolished the effect of cAMP on NHE-3 activity demonstrating a role of PKA in the functional regulation of NHE-3 by cAMP ... We conclude that regulation of NHE-3 by PKA in vivo involves complex mechanisms, which include phosphorylation of Ser-552 and Ser-605
Zanger et al., Mol Endocrinol 1999 : The pituitary-specific transcription factor , Pit-1, is necessary to mediate protein kinase A (PKA) regulation of the GH, PRL, and TSH-beta subunit genes in the pituitary ... The pituitary-specific transcription factor, Pit-1, is necessary to mediate protein kinase A (PKA) regulation of the GH, PRL , and TSH-beta subunit genes in the pituitary
Li et al., Exp Eye Res 1999 (Second Messenger Systems) : SQ22536 ( 100 microM ), an adenylate cyclase inhibitor, and H-89, a PKA inhibitor , had no inhibitory effect on bFGF induced down-regulation of IGF-I expression