| CIP-TAP |
This kind of library was made from RNA < 200 nucleotides that was pre-treated sequentially with Calf Intestinal Alkaline Phosphatase followed by Tobacco Acid Pyrophosphatase. Hence, it will be enriched for capped RNAs. |
CIP-TAP |
| FASP |
Filter-Assisted Sample Preparation (FASP) proteomics protocol (Giddings) |
FASP |
| In-gel |
In-gel digestion proteomics protocol (Giddings) |
In-gel digestion |
| MudPIT |
Multidimensional Protein Identification Technology (MudPIT) proteomics protocol (Giddings) |
MudPIT |
| PCR1x |
one 15-cycle round of PCR (Myers) |
PCR 1-round |
| PCR2x |
a 25-cycle round of PCR and an additional 15-cycle round of PCR after gel size selection (Myers) |
PCR 2-round |
| SDS-Page |
SDS-Page proteomics sample preparation protocol (Giddings) |
SDS-Page proteomics sample |
| TAP-Only |
This kind of library was made from RNA < 200 nucleotides that were pre-treated with Tobacco Acid Pyrophosphatase to remove any 5' caps that would preclude cloning. Hence, both capped and 5' monophosphate RNAs will be cloned. |
Tobacco Acid Pyrophosphatase Only |
| v041610.1 |
2x10^7 cells, fragmentation by bioruptor, one 15-cycle round of PCR (Myers) |
biorupter, PCR 1-round |
| v041610.2 |
2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers) |
sonicator, PCR 1-round |
| v042211.1 |
Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers) |
ChIP AMpure XP |
| v042211.2 |
Faster ChIP protocol & gel size selection for ChIP-seq (Myers) |
ChIP gel |