Human Gene AGPAT3 (ENST00000291572.13_4) from GENCODE V47lift37
  Description: 1-acylglycerol-3-phosphate O-acyltransferase 3, transcript variant 1 (from RefSeq NM_020132.5)
Gencode Transcript: ENST00000291572.13_4
Gencode Gene: ENSG00000160216.21_11
Transcript (Including UTRs)
   Position: hg19 chr21:45,285,104-45,407,473 Size: 122,370 Total Exon Count: 10 Strand: +
Coding Region
   Position: hg19 chr21:45,379,563-45,402,273 Size: 22,711 Coding Exon Count: 8 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr21:45,285,104-45,407,473)mRNA (may differ from genome)Protein (376 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedReactomeUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: PLCC_HUMAN
DESCRIPTION: RecName: Full=1-acyl-sn-glycerol-3-phosphate acyltransferase gamma; EC=2.3.1.51; AltName: Full=1-acylglycerol-3-phosphate O-acyltransferase 3; Short=1-AGP acyltransferase 3; Short=1-AGPAT 3; AltName: Full=Lysophosphatidic acid acyltransferase gamma; Short=LPAAT-gamma;
FUNCTION: Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone (By similarity).
CATALYTIC ACTIVITY: Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.
PATHWAY: Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3.
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein.
DOMAIN: The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate (By similarity).
SIMILARITY: Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 22.04 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 589.25 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -126.50234-0.541 Picture PostScript Text
3' UTR -1853.045200-0.356 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR002123 - Acyltransferase

Pfam Domains:
PF01553 - Acyltransferase
PF16076 - Acyltransferase C-terminus

SCOP Domains:
51556 - Metallo-dependent hydrolases
69593 - Glycerol-3-phosphate (1)-acyltransferase

ModBase Predicted Comparative 3D Structure on Q9NRZ7
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003841 1-acylglycerol-3-phosphate O-acyltransferase activity
GO:0016740 transferase activity
GO:0016746 transferase activity, transferring acyl groups
GO:0042171 lysophosphatidic acid acyltransferase activity

Biological Process:
GO:0006629 lipid metabolic process
GO:0006654 phosphatidic acid biosynthetic process
GO:0008152 metabolic process
GO:0008654 phospholipid biosynthetic process
GO:0016024 CDP-diacylglycerol biosynthetic process

Cellular Component:
GO:0000139 Golgi membrane
GO:0005634 nucleus
GO:0005635 nuclear envelope
GO:0005783 endoplasmic reticulum
GO:0005789 endoplasmic reticulum membrane
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  AY358704 - Homo sapiens clone DNA71213 AGPAT3 (UNQ759) mRNA, complete cds.
BC011971 - Homo sapiens 1-acylglycerol-3-phosphate O-acyltransferase 3, mRNA (cDNA clone MGC:16906 IMAGE:4148915), complete cds.
AF156774 - Homo sapiens lysophosphatidic acid acyltransferase-gamma1 (LPAAT-gamma1) mRNA, complete cds.
AB040138 - Homo sapiens AGPAT3 mRNA for 1-acylglycerol-3-phosphate O-acyltransferase 3, complete cds.
BC063552 - Homo sapiens 1-acylglycerol-3-phosphate O-acyltransferase 3, mRNA (cDNA clone MGC:75467 IMAGE:4800514), complete cds.
AK300562 - Homo sapiens cDNA FLJ61409 complete cds, highly similar to 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma (EC 2.3.1.51).
JD310243 - Sequence 291267 from Patent EP1572962.
JD252211 - Sequence 233235 from Patent EP1572962.
BC040603 - Homo sapiens 1-acylglycerol-3-phosphate O-acyltransferase 3, mRNA (cDNA clone MGC:43669 IMAGE:5270905), complete cds.
AB587497 - Synthetic construct DNA, clone: pF1KB9185, Homo sapiens AGPAT3 gene for 1-acylglycerol-3-phosphate O-acyltransferase 3, without stop codon, in Flexi system.
CU687760 - Synthetic construct Homo sapiens gateway clone IMAGE:100022765 5' read AGPAT3 mRNA.
HQ447507 - Synthetic construct Homo sapiens clone IMAGE:100070844; CCSB014222_01 1-acylglycerol-3-phosphate O-acyltransferase 3 (AGPAT3) gene, encodes complete protein.
KJ894355 - Synthetic construct Homo sapiens clone ccsbBroadEn_03749 AGPAT3 gene, encodes complete protein.
KJ899260 - Synthetic construct Homo sapiens clone ccsbBroadEn_08654 AGPAT3 gene, encodes complete protein.
AF156775 - Homo sapiens lysophosphatidic acid acyltransferase-gamma2 (LPAAT-gamma2) mRNA, complete cds.
AK125804 - Homo sapiens cDNA FLJ43816 fis, clone TESTI4001561, highly similar to 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma (EC 2.3.1.51).
AL832919 - Homo sapiens mRNA; cDNA DKFZp762M1415 (from clone DKFZp762M1415).
JD161095 - Sequence 142119 from Patent EP1572962.
JD342985 - Sequence 324009 from Patent EP1572962.
JD173261 - Sequence 154285 from Patent EP1572962.
AL109795 - Homo sapiens mRNA full length insert cDNA clone EUROIMAGE 180147.
AK026135 - Homo sapiens cDNA: FLJ22482 fis, clone HRC10859, highly similar to IRO180147 Homo sapiens mRNA full length insert cDNA clone EUROIMAGE 180147.
BC026047 - Homo sapiens 1-acylglycerol-3-phosphate O-acyltransferase 3, mRNA (cDNA clone IMAGE:4846056), with apparent retained intron.
BC013167 - Homo sapiens 1-acylglycerol-3-phosphate O-acyltransferase 3, mRNA (cDNA clone IMAGE:4346467), with apparent retained intron.
BC004413 - Homo sapiens 1-acylglycerol-3-phosphate O-acyltransferase 3, mRNA (cDNA clone IMAGE:3640200).
AL355720 - Homo sapiens mRNA full length insert cDNA clone EUROIMAGE 42408.
BC004219 - Homo sapiens 1-acylglycerol-3-phosphate O-acyltransferase 3, mRNA (cDNA clone IMAGE:3354271).
AK074300 - Homo sapiens cDNA FLJ23720 fis, clone HEP13757.
KJ906255 - Synthetic construct Homo sapiens clone ccsbBroadEn_15925 AGPAT3 gene, encodes complete protein.
AL355721 - Homo sapiens EST from clone 41529, 5' end.
JD359962 - Sequence 340986 from Patent EP1572962.
JD213400 - Sequence 194424 from Patent EP1572962.
JD213401 - Sequence 194425 from Patent EP1572962.
JD399785 - Sequence 380809 from Patent EP1572962.
JD039989 - Sequence 21013 from Patent EP1572962.
JD335779 - Sequence 316803 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  BioCyc Knowledge Library
PWY-5667 - CDP-diacylglycerol biosynthesis
TRIGLSYN-PWY - diacylglycerol and triacylglycerol biosynthesis

Reactome (by CSHL, EBI, and GO)

Protein Q9NRZ7 (Reactome details) participates in the following event(s):

R-HSA-8849345 LPAAT3 acylates lysophosphatidylcholine to yield phosphatidylcholine
R-HSA-75885 1-acyl LPA is acylated to PA by AGPAT (LPAAT)
R-HSA-6811436 COPI-independent Golgi-to-ER retrograde traffic
R-HSA-1483166 Synthesis of PA
R-HSA-8856688 Golgi-to-ER retrograde transport
R-HSA-1483206 Glycerophospholipid biosynthesis
R-HSA-6811442 Intra-Golgi and retrograde Golgi-to-ER traffic
R-HSA-1483257 Phospholipid metabolism
R-HSA-199991 Membrane Trafficking
R-HSA-556833 Metabolism of lipids
R-HSA-5653656 Vesicle-mediated transport
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: D3DSL2, ENST00000291572.1, ENST00000291572.10, ENST00000291572.11, ENST00000291572.12, ENST00000291572.2, ENST00000291572.3, ENST00000291572.4, ENST00000291572.5, ENST00000291572.6, ENST00000291572.7, ENST00000291572.8, ENST00000291572.9, LPAAT3, NM_020132, PLCC_HUMAN, Q3ZCU2, Q6UWP6, Q6ZUC6, Q8N3Q7, Q9NRZ6, Q9NRZ7, uc317kyh.1, uc317kyh.2, UNQ759/PRO1490
UCSC ID: ENST00000291572.13_4
RefSeq Accession: NM_020132.5
Protein: Q9NRZ7 (aka PLCC_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.