Human Gene CARD16 (ENST00000525374.1_9) from GENCODE V47lift37
  Description: Caspase inhibitor. Acts as a regulator of procaspase-1/CASP1 activation implicated in the regulation of the proteolytic maturation of pro-interleukin-1 beta (IL1B) and its release during inflammation. Inhibits the release of IL1B in response to LPS in monocytes. Also induces NF-kappa-B activation during the pro-inflammatory cytokine response. Also able to inhibit CASP1-mediated neuronal cell death, TNF- alpha, hypoxia-, UV-, and staurosporine-mediated cell death but not ER stress-mediated cell death. Acts by preventing activation of caspases CASP1 and CASP4, possibly by preventing the interaction between CASP1 and RIPK2. (from UniProt Q5EG05)
Gencode Transcript: ENST00000525374.1_9
Gencode Gene: ENSG00000204397.11_16
Transcript (Including UTRs)
   Position: hg19 chr11:104,912,298-104,972,158 Size: 59,861 Total Exon Count: 4 Strand: -


Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsOther NamesModel Information
Methods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr11:104,912,298-104,972,158)mRNA (may differ from genome)No protein
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Wikipedia

-  Comments and Description Text from UniProtKB
  ID: CAR16_HUMAN
DESCRIPTION: RecName: Full=Caspase recruitment domain-containing protein 16; AltName: Full=CARD only domain-containing protein 1; AltName: Full=Caspase-1 inhibitor COP; AltName: Full=Pseudo interleukin-1 beta converting enzyme; Short=Pseudo-ICE;
FUNCTION: Caspase inhibitor. Acts as a regulator of procaspase- 1/CASP1 activation implicated in the regulation of the proteolytic maturation of pro-interleukin-1 beta (IL1B) and its release during inflammation. Inhibits the release of IL1B in response to LPS in monocytes. Also induces NF-kappa-B activation during the pro- inflammatory cytokine response. Also able to inhibit CASP1- mediated neuronal cell death, TNF-alpha, hypoxia-, UV-, and staurosporine-mediated cell death but not ER stress-mediated cell death. Acts by preventing activation of caspases CASP1 and CASP4, possibly by preventing the interaction between CASP1 and RIPK2.
SUBUNIT: Homooligomer. Interacts with CASP1, CASP4, CARD8 and RIPK2.
TISSUE SPECIFICITY: Widely expressed. Expressed at higher level in placenta, spleen, lymph node and bone marrow. Weakly or not expressed in thymus.
INDUCTION: Down-regulated in patients suffering of Huntington disease.
SIMILARITY: Contains 1 CARD domain.

-  Primer design for this transcript
 

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-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 45.77 RPKM in Spleen
Total median expression: 268.18 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001315 - CARD
IPR011029 - DEATH-like

ModBase Predicted Comparative 3D Structure on Q5EG05
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
      
      
      
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004869 cysteine-type endopeptidase inhibitor activity
GO:0005515 protein binding
GO:0019900 kinase binding
GO:0030414 peptidase inhibitor activity
GO:0042802 identical protein binding
GO:0050700 CARD domain binding
GO:0089720 caspase binding

Biological Process:
GO:0010466 negative regulation of peptidase activity
GO:0010804 negative regulation of tumor necrosis factor-mediated signaling pathway
GO:0031665 negative regulation of lipopolysaccharide-mediated signaling pathway
GO:0032091 negative regulation of protein binding
GO:0042981 regulation of apoptotic process
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB signaling
GO:0043154 negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
GO:0050713 negative regulation of interleukin-1 beta secretion
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0071222 cellular response to lipopolysaccharide
GO:0071456 cellular response to hypoxia
GO:0071494 cellular response to UV-C
GO:0097340 inhibition of cysteine-type endopeptidase activity

Cellular Component:
GO:0032991 macromolecular complex
GO:0097179 protease inhibitor complex


-  Descriptions from all associated GenBank mRNAs
  AY885669 - Homo sapiens CARD only protein (COP1) mRNA, complete cds, alternatively spliced.
BC148760 - Synthetic construct Homo sapiens clone IMAGE:100015834, MGC:183188 caspase recruitment domain family, member 16 (CARD16) mRNA, encodes complete protein.
BC141473 - Synthetic construct Homo sapiens clone IMAGE:100014731, MGC:175355 caspase recruitment domain family, member 16 (CARD16) mRNA, encodes complete protein.
AB463466 - Synthetic construct DNA, clone: pF1KB7370, Homo sapiens COP1 gene for caspase-1 dominant-negative inhibitor pseudo-ICE isoform 2, without stop codon, in Flexi system.
AK125640 - Homo sapiens cDNA FLJ43652 fis, clone SYNOV4002001, weakly similar to Homo sapiens caspase 1, apoptosis-related cysteine protease (interleukin 1, beta, convertase) (CASP1).
BC070196 - Homo sapiens caspase recruitment domain family, member 16, mRNA (cDNA clone IMAGE:6652663).
BC104635 - Homo sapiens caspase recruitment domain family, member 16, mRNA (cDNA clone IMAGE:4703925).
AF367017 - Homo sapiens caspase-1 dominant-negative inhibitor Pseudo-ICE mRNA, complete cds.
JD511104 - Sequence 492128 from Patent EP1572962.
JD138698 - Sequence 119722 from Patent EP1572962.
BC117478 - Homo sapiens caspase recruitment domain family, member 16, mRNA (cDNA clone IMAGE:40126029), complete cds.
BC117480 - Homo sapiens caspase recruitment domain family, member 16, mRNA (cDNA clone IMAGE:40126031), complete cds.
BC033638 - Homo sapiens caspase recruitment domain family, member 16, mRNA (cDNA clone IMAGE:5221546), with apparent retained intron.
JD415304 - Sequence 396328 from Patent EP1572962.
AK311902 - Homo sapiens cDNA, FLJ92168, Homo sapiens CARD only protein (COP), mRNA.
AY761064 - Homo sapiens INCA (INCA) mRNA, complete cds.

-  Other Names for This Gene
  Alternate Gene Symbols: BC104635, CAR16_HUMAN, COP, COP1, Q5EG05, Q96RJ9, uc324amx.1
UCSC ID: ENST00000525374.1_9
RefSeq Accession: NM_052889.4
Protein: Q5EG05 (aka CAR16_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.