Human Gene EEF2 (ENST00000309311.7_7) from GENCODE V47lift37
  Description: eukaryotic translation elongation factor 2 (from RefSeq NM_001961.4)
Gencode Transcript: ENST00000309311.7_7
Gencode Gene: ENSG00000167658.16_9
Transcript (Including UTRs)
   Position: hg19 chr19:3,976,054-3,985,461 Size: 9,408 Total Exon Count: 15 Strand: -
Coding Region
   Position: hg19 chr19:3,976,552-3,985,378 Size: 8,827 Coding Exon Count: 15 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesGeneReviewsModel Information
Methods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr19:3,976,054-3,985,461)mRNA (may differ from genome)Protein (858 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: EF2_HUMAN
DESCRIPTION: RecName: Full=Elongation factor 2; Short=EF-2;
FUNCTION: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
SUBUNIT: Component of the mRNA surveillance SURF complex, at least composed of ERF1, ERF3 (ERF3A or ERF3B), EEF2, UPF1/RENT1, SMG1, SMG8 and SMG9.
SUBCELLULAR LOCATION: Cytoplasm.
PTM: Phosphorylation by EF-2 kinase completely inactivates EF-2.
PTM: Diphthamide is 2-[3-carboxyamido-3-(trimethyl- ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis.
PTM: ISGylated.
SIMILARITY: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: EEF2
Diseases sorted by gene-association score: spinocerebellar ataxia 26* (1734), diphtheria (48), pancoast tumor (15), primary bacterial infectious disease (9), fusariosis (9), myotonic dystrophy 2 (9)
* = Manually curated disease association

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 1126.22 RPKM in Ovary
Total median expression: 27587.68 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -23.2083-0.280 Picture PostScript Text
3' UTR -165.70498-0.333 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR009022 - Elongation_fac_G/III/V
IPR000795 - ProtSyn_GTP-bd
IPR020568 - Ribosomal_S5_D2-typ_fold
IPR014721 - Ribosomal_S5_D2-typ_fold_subgr
IPR005225 - Small_GTP-bd_dom
IPR000640 - Transl_elong_EFG/EF2_C
IPR005517 - Transl_elong_EFG/EF2_IV
IPR004161 - Transl_elong_EFTu/EF1A_2
IPR009000 - Transl_elong_init/rib_B-barrel

Pfam Domains:
PF00009 - Elongation factor Tu GTP binding domain
PF00679 - Elongation factor G C-terminus
PF01926 - 50S ribosome-binding GTPase
PF03144 - Elongation factor Tu domain 2
PF03764 - Elongation factor G, domain IV
PF14492 - Elongation Factor G, domain III

SCOP Domains:
50447 - Translation proteins
52540 - P-loop containing nucleoside triphosphate hydrolases
54211 - Ribosomal protein S5 domain 2-like
54980 - EF-G C-terminal domain-like

ModBase Predicted Comparative 3D Structure on P13639
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologGenome Browser
Gene DetailsGene Details Gene Details Gene Details
Gene SorterGene Sorter Gene Sorter Gene Sorter
 RGDEnsembl  SGD
     Protein Sequence
     Alignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0002039 p53 binding
GO:0003723 RNA binding
GO:0003746 translation elongation factor activity
GO:0003924 GTPase activity
GO:0005515 protein binding
GO:0005525 GTP binding
GO:0008097 5S rRNA binding
GO:0019901 protein kinase binding
GO:0043022 ribosome binding
GO:0045296 cadherin binding
GO:0051015 actin filament binding

Biological Process:
GO:0002244 hematopoietic progenitor cell differentiation
GO:0002931 response to ischemia
GO:0003009 skeletal muscle contraction
GO:0006412 translation
GO:0006414 translational elongation
GO:0007568 aging
GO:0014009 glial cell proliferation
GO:0032355 response to estradiol
GO:0034976 response to endoplasmic reticulum stress
GO:0035914 skeletal muscle cell differentiation
GO:0042493 response to drug
GO:0042542 response to hydrogen peroxide
GO:0043312 neutrophil degranulation
GO:0045471 response to ethanol
GO:0045727 positive regulation of translation
GO:0051593 response to folic acid
GO:1990416 cellular response to brain-derived neurotrophic factor stimulus
GO:2000767 positive regulation of cytoplasmic translation

Cellular Component:
GO:0005576 extracellular region
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005844 polysome
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0016235 aggresome
GO:0034774 secretory granule lumen
GO:0042788 polysomal ribosome
GO:0045121 membrane raft
GO:0070062 extracellular exosome
GO:1904813 ficolin-1-rich granule lumen
GO:1990904 ribonucleoprotein complex


-  Descriptions from all associated GenBank mRNAs
  BC024689 - Homo sapiens, Similar to Elongation factor 2b, clone IMAGE:4153410, mRNA, partial cds.
Z11692 - H.sapiens mRNA for elongation factor 2.
M19997 - Human elongation factor 2 (EF-2) mRNA, 3' end.
X51466 - Human mRNA for elongation factor 2.
JD289671 - Sequence 270695 from Patent EP1572962.
BC126259 - Homo sapiens eukaryotic translation elongation factor 2, mRNA (cDNA clone MGC:161537 IMAGE:8991975), complete cds.
BC136313 - Homo sapiens eukaryotic translation elongation factor 2, mRNA (cDNA clone MGC:167923 IMAGE:9020300), complete cds.
GQ901037 - Homo sapiens clone HEL-T-149 epididymis secretory sperm binding protein mRNA, complete cds.
JD124655 - Sequence 105679 from Patent EP1572962.
JD355558 - Sequence 336582 from Patent EP1572962.
JD236871 - Sequence 217895 from Patent EP1572962.
DL492547 - Novel nucleic acids.
JD434525 - Sequence 415549 from Patent EP1572962.
JD120685 - Sequence 101709 from Patent EP1572962.
JD472141 - Sequence 453165 from Patent EP1572962.
JD278135 - Sequence 259159 from Patent EP1572962.
AK299225 - Homo sapiens cDNA FLJ58164 complete cds, highly similar to Elongation factor 2.
AK298498 - Homo sapiens cDNA FLJ56548 complete cds, highly similar to Elongation factor 2.
AK297398 - Homo sapiens cDNA FLJ60696 complete cds, highly similar to Elongation factor 2.
JD057419 - Sequence 38443 from Patent EP1572962.
AY942181 - Homo sapiens elongation factor 2 mRNA, complete cds.
HQ258667 - Synthetic construct Homo sapiens clone IMAGE:100072697 eukaryotic translation elongation factor 2 (EEF2) gene, encodes complete protein.
KJ896748 - Synthetic construct Homo sapiens clone ccsbBroadEn_06142 EEF2 gene, encodes complete protein.
AB527843 - Synthetic construct DNA, clone: pF1KB8492, Homo sapiens EEF2 gene for eukaryotic translation elongation factor 2, without stop codon, in Flexi system.
DL492055 - Novel nucleic acids.
DL490604 - Novel nucleic acids.
DL491963 - Novel nucleic acids.
DL490520 - Novel nucleic acids.
BC006547 - Homo sapiens eukaryotic translation elongation factor 2, mRNA (cDNA clone IMAGE:2963048), partial cds.
BC068002 - Homo sapiens cDNA clone IMAGE:6052873, **** WARNING: chimeric clone ****.
DQ587503 - Homo sapiens piRNA piR-54615, complete sequence.
JD174253 - Sequence 155277 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  BioCarta from NCI Cancer Genome Anatomy Project
h_eifPathway - Eukaryotic protein translation

Reactome (by CSHL, EBI, and GO)

Protein P13639 (Reactome details) participates in the following event(s):

R-HSA-156930 GTP-binding activates eEF2
R-HSA-5336421 DT fragment A ADP-ribosylates target cell EEF
R-HSA-8932243 EEF2KMT transfers 3xCH3 from 3xAdoMet to EEF2
R-HSA-5358475 DPH6 ligates ammonium to diphthine-EEF2
R-HSA-5358494 DPH2 transfers a 3-amino-3-carboxypropyl group from AdoMet to residue 715 of nascent EEF2
R-HSA-5358484 DPH5 transfers four methyl groups from AdoMet to aminocarboxypropyl EEF2
R-HSA-5367022 DPH7 hydrolyzes a methyl group on Me-diphthine EEF2
R-HSA-156915 Translocation of ribosome by 3 bases in the 3' direction
R-HSA-6798748 Exocytosis of secretory granule lumen proteins
R-HSA-6800434 Exocytosis of ficolin-rich granule lumen proteins
R-HSA-156902 Peptide chain elongation
R-HSA-5336415 Uptake and function of diphtheria toxin
R-HSA-8876725 Protein methylation
R-HSA-5358493 Synthesis of diphthamide-EEF2
R-HSA-156842 Eukaryotic Translation Elongation
R-HSA-5339562 Uptake and actions of bacterial toxins
R-HSA-597592 Post-translational protein modification
R-HSA-163841 Gamma carboxylation, hypusine formation and arylsulfatase activation
R-HSA-6798695 Neutrophil degranulation
R-HSA-72766 Translation
R-HSA-5663205 Infectious disease
R-HSA-392499 Metabolism of proteins
R-HSA-168249 Innate Immune System
R-HSA-1643685 Disease
R-HSA-168256 Immune System

-  Other Names for This Gene
  Alternate Gene Symbols: A0A384N6H1, B2RMP5, D6W618, EF2, EF2_HUMAN, ENST00000309311.1, ENST00000309311.2, ENST00000309311.3, ENST00000309311.4, ENST00000309311.5, ENST00000309311.6, NM_001961, P13639, Q58J86, uc317omt.1, uc317omt.2
UCSC ID: ENST00000309311.7_7
RefSeq Accession: NM_001961.4
Protein: P13639 (aka EF2_HUMAN)

-  GeneReviews for This Gene
  GeneReviews article(s) related to gene EEF2:
ataxias (Hereditary Ataxia Overview)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.