Human Gene CXCL8 (ENST00000307407.8_6) from GENCODE V47lift37
  Description: C-X-C motif chemokine ligand 8, transcript variant 1 (from RefSeq NM_000584.4)
Gencode Transcript: ENST00000307407.8_6
Gencode Gene: ENSG00000169429.12_11
Transcript (Including UTRs)
   Position: hg19 chr4:74,606,286-74,609,433 Size: 3,148 Total Exon Count: 4 Strand: +
Coding Region
   Position: hg19 chr4:74,606,376-74,608,181 Size: 1,806 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr4:74,606,286-74,609,433)mRNA (may differ from genome)Protein (99 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMalacards
MGIOMIMPubMedReactomeUniProtKBWikipedia
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: IL8_HUMAN
DESCRIPTION: RecName: Full=Interleukin-8; Short=IL-8; AltName: Full=C-X-C motif chemokine 8; AltName: Full=Emoctakin; AltName: Full=Granulocyte chemotactic protein 1; Short=GCP-1; AltName: Full=Monocyte-derived neutrophil chemotactic factor; Short=MDNCF; AltName: Full=Monocyte-derived neutrophil-activating peptide; Short=MONAP; AltName: Full=Neutrophil-activating protein 1; Short=NAP-1; AltName: Full=Protein 3-10C; AltName: Full=T-cell chemotactic factor; Contains: RecName: Full=MDNCF-a; AltName: Full=GCP/IL-8 protein IV; AltName: Full=IL8/NAP1 form I; Contains: RecName: Full=Interleukin-8; AltName: Full=(Ala-IL-8)77; AltName: Full=GCP/IL-8 protein II; AltName: Full=IL-8(1-77); AltName: Full=IL8/NAP1 form II; AltName: Full=MDNCF-b; Contains: RecName: Full=IL-8(5-77); Contains: RecName: Full=IL-8(6-77); AltName: Full=(Ser-IL-8)72; AltName: Full=GCP/IL-8 protein I; AltName: Full=IL8/NAP1 form III; AltName: Full=Lymphocyte-derived neutrophil-activating factor; Short=LYNAP; AltName: Full=MDNCF-c; AltName: Full=Neutrophil-activating factor; Short=NAF; Contains: RecName: Full=IL-8(7-77); AltName: Full=GCP/IL-8 protein V; AltName: Full=IL8/NAP1 form IV; Contains: RecName: Full=IL-8(8-77); AltName: Full=GCP/IL-8 protein VI; AltName: Full=IL8/NAP1 form V; Contains: RecName: Full=IL-8(9-77); AltName: Full=GCP/IL-8 protein III; AltName: Full=IL8/NAP1 form VI; Flags: Precursor;
FUNCTION: IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
SUBUNIT: Homodimer.
SUBCELLULAR LOCATION: Secreted.
INDUCTION: By ER stress in a DDIT3/CHOP-dependent manner.
PTM: Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
SIMILARITY: Belongs to the intercrine alpha (chemokine CxC) family.
WEB RESOURCE: Name=Wikipedia; Note=Interleukin-8 entry; URL="http://en.wikipedia.org/wiki/Interleukin_8";
WEB RESOURCE: Name=SeattleSNPs; URL="http://pga.gs.washington.edu/data/il8/";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: CXCL8
Diseases sorted by gene-association score: bronchiolitis (40), extrinsic allergic alveolitis (34), lung giant cell carcinoma (31), bronchiolitis obliterans (31), adult respiratory distress syndrome (25), acute respiratory distress syndrome (24), aseptic meningitis (23), bacterial vaginosis (22), denture stomatitis (22), cryptogenic organizing pneumonia (22), bagassosis (21), alcoholic hepatitis (21), meningitis (21), gastritis (20), toxicodendron dermatitis (19), chorioamnionitis (19), erythema elevatum diutinum (19), acute transverse myelitis (18), bronchitis (18), mycobacterium kansasii (18), recurrent corneal erosion (18), peptic ulcer disease (17), mastitis (17), pneumonia (17), funisitis (16), bacteriuria (16), meconium aspiration syndrome (16), necrotizing fasciitis (16), acne (14), bacterial meningitis (14), pyelonephritis (14), pulpitis (13), haemophilus influenzae (13), common cold (13), acute generalized exanthematous pustulosis (13), herpes simplex virus keratitis (13), appendicitis (13), mycobacterium chelonae (13), mycobacterium fortuitum (13), mycobacterium marinum (13), toxic shock syndrome (12), acute pyelonephritis (12), vulvovaginitis (12), lung disease (12), arthritis (11), periodontal disease (11), pyoderma (11), allergic rhinitis (11), duodenal ulcer (11), papillary conjunctivitis (11), bronchiectasis (11), interstitial lung disease (10), synovitis (10), pulmonary edema (10), tuberculous meningitis (10), bronchopulmonary dysplasia (10), noma (10), acute monoblastic leukemia (10), opisthorchiasis (10), cardiac rupture (10), engraftment syndrome (10), endometriosis (10), prostatitis (10), giant papillary conjunctivitis (10), capillary leak syndrome (10), cystic fibrosis (9), vibratory urticaria (9), vaginal disease (9), sapho syndrome (9), alcoholic liver cirrhosis (9), pleurisy (9), trypanosomiasis (9), streptococcal toxic-shock syndrome (9), cerebral folate deficiency (9), invasive aspergillosis (9), clonorchiasis (9), pyoderma gangrenosum (9), ulcerative colitis (9), periapical granuloma (9), pharyngitis (9), gastroenteritis (9), rhinitis (8), pulmonary disease, chronic obstructive (8), inflammatory diarrhea (8), septic arthritis (8), cystitis (8), acute pancreatitis (8), fasciitis (8), crohn's colitis (8), oral candidiasis (8), autoinflammation, lipodystrophy, and dermatosis syndrome (8), shigellosis (8), felty syndrome (8), idiopathic interstitial pneumonia (8), periodontitis (8), pneumoconiosis (8), alpha 1-antitrypsin deficiency (8), scabies (8), exudative glomerulonephritis (8), pleural tuberculosis (8), hypereosinophilic syndrome (7), chlamydia (7), severe acute respiratory syndrome (7), status asthmaticus (7), otitis media (7), gastroesophageal reflux (7), estrogen-receptor negative breast cancer (7), gingivitis (7), pouchitis (7), leptospirosis (7), spinal cord injury (7), primary peritoneal carcinoma (7), keratitis (7), aggressive periodontitis (7), allergic conjunctivitis (7), conjunctivitis (7), rheumatoid arthritis (7), atrophic gastritis (7), peritonitis (7), trichomoniasis (7), bullous keratopathy (7), respiratory system disease (7), gastric ulcer (7), esophagitis (7), viral meningitis (7), uterine disease (6), acute chest syndrome (6), chagas disease (6), transverse myelitis (6), microvascular complications of diabetes 1 (6), monocytic leukemia (6), tonsillitis (6), interstitial cystitis (6), contact dermatitis (6), dengue hemorrhagic fever (6), brucellosis (6), cowpox (6), lymphadenitis (6), slow-channel congenital myasthenic syndrome (6), dyspepsia (6), peptic esophagitis (6), trichuriasis (6), endometrial disease (6), endocarditis (6), tungiasis (6), necatoriasis (6), allergic contact dermatitis (6), silicosis (6), keratoconjunctivitis (6), periodontosis (6), chondroblastoma (6), sinusitis (6), toxic myocarditis (6), osteomyelitis (6), mycobacterium abscessus (6), pyuria (6), obstructive jaundice (6), carotid stenosis (6), sebaceous gland disease (5), sleep apnea (5), vaginitis (5), preterm premature rupture of the membranes (5), retinitis pigmentosa 44 (5), cytomegalovirus infection (5), common wart (5), viral encephalitis (5), visceral leishmaniasis (5), obstructive sleep apnea (5), wells syndrome (5), stomach disease (5), wegener granulomatosis (5), israeli tick typhus (5), myxoid liposarcoma (5), radiculopathy (5), dental pulp disease (5), pulmonary fibrosis, idiopathic (5), mycetoma (5), acute cystitis (5), perinatal necrotizing enterocolitis (5), colitis (5), upper respiratory tract disease (5), malaria (5), kaposi sarcoma (5), ovarian lymphoma (5), female reproductive system disease (5), bronchial disease (5), placenta disease (4), fatty liver disease (4), hereditary hemorrhagic telangiectasia (4), cervix disease (4), middle ear disease (4), louse-borne relapsing fever (4), behcet syndrome (4), nasal cavity disease (4), nose disease (4), inflammatory bowel disease (4), allergic asthma (4), osteoarthritis (4), spermatocele (4), hepatocellular carcinoma (3), asthma (3), eye disease (3), chronic fatigue syndrome (3), oral squamous cell carcinoma (3), kidney cancer (2), hemophagocytic lymphohistiocytosis (2), stomach cancer (2), pancreatic cancer (2), viral infectious disease (2), squamous cell carcinoma, head and neck (2), esophageal cancer (2), bone inflammation disease (1), gastrointestinal system disease (1), endometrial stromal sarcoma (1), reproductive system disease (1), intestinal disease (1), colorectal cancer (1), macular degeneration, age-related, 1 (1)

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 17.44 RPKM in Lung
Total median expression: 79.03 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -14.1090-0.157 Picture PostScript Text
3' UTR -267.601252-0.214 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001089 - Chemokine_CXC
IPR018048 - Chemokine_CXC_CS
IPR002473 - Chemokine_CXCL8/IL8
IPR001811 - Chemokine_IL8-like_dom

Pfam Domains:
PF00048 - Small cytokines (intecrine/chemokine), interleukin-8 like

SCOP Domains:
54117 - Interleukin 8-like chemokines

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1ICW - X-ray MuPIT 1IKL - NMR MuPIT 1IKM - NMR MuPIT 1IL8 - NMR MuPIT 1ILP - NMR MuPIT 1ILQ - NMR MuPIT 1QE6 - X-ray MuPIT 1ROD - NMR MuPIT 2IL8 - NMR MuPIT 3IL8 - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P10145
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
      
      
  Ensembl   
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005125 cytokine activity
GO:0005153 interleukin-8 receptor binding
GO:0005515 protein binding
GO:0008009 chemokine activity

Biological Process:
GO:0001525 angiogenesis
GO:0002237 response to molecule of bacterial origin
GO:0006935 chemotaxis
GO:0006952 defense response
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0007050 cell cycle arrest
GO:0007165 signal transduction
GO:0007186 G-protein coupled receptor signaling pathway
GO:0008285 negative regulation of cell proliferation
GO:0010469 regulation of receptor activity
GO:0019221 cytokine-mediated signaling pathway
GO:0019722 calcium-mediated signaling
GO:0030155 regulation of cell adhesion
GO:0030593 neutrophil chemotaxis
GO:0031623 receptor internalization
GO:0031640 killing of cells of other organism
GO:0034976 response to endoplasmic reticulum stress
GO:0035556 intracellular signal transduction
GO:0036499 PERK-mediated unfolded protein response
GO:0042119 neutrophil activation
GO:0044344 cellular response to fibroblast growth factor stimulus
GO:0045091 regulation of single stranded viral RNA replication via double stranded DNA intermediate
GO:0045744 negative regulation of G-protein coupled receptor protein signaling pathway
GO:0045766 positive regulation of angiogenesis
GO:0048566 embryonic digestive tract development
GO:0050930 induction of positive chemotaxis
GO:0070098 chemokine-mediated signaling pathway
GO:0071222 cellular response to lipopolysaccharide
GO:0071347 cellular response to interleukin-1
GO:0071356 cellular response to tumor necrosis factor
GO:0090023 positive regulation of neutrophil chemotaxis
GO:2000535 regulation of entry of bacterium into host cell

Cellular Component:
GO:0005576 extracellular region
GO:0005615 extracellular space
GO:0005622 intracellular


-  Descriptions from all associated GenBank mRNAs
  LP896310 - Sequence 1174 from Patent EP3253886.
LQ882878 - Sequence 27 from Patent WO2018160841.
Y00787 - Human mRNA for MDNCF (monocyte-derived neutrophil chemotactic factor).
AK311874 - Homo sapiens cDNA, FLJ92140, Homo sapiens interleukin 8 (IL8), mRNA.
AK131067 - Homo sapiens cDNA FLJ29033 fis, clone DMC06975.
M17017 - Human beta-thromboglobulin-like protein mRNA, complete cds.
JD042176 - Sequence 23200 from Patent EP1572962.
M26383 - Human monocyte-derived neutrophil-activating protein (MONAP) mRNA, complete cds.
JD361648 - Sequence 342672 from Patent EP1572962.
BC013615 - Homo sapiens interleukin 8, mRNA (cDNA clone MGC:9211 IMAGE:3882471), complete cds.
AF043337 - Homo sapiens interleukin 8 C-terminal variant (IL8) mRNA, complete cds.
AB590232 - Synthetic construct DNA, clone: pFN21AE1361, Homo sapiens IL8 gene for interleukin 8, without stop codon, in Flexi system.
DQ893727 - Synthetic construct Homo sapiens clone IMAGE:100008187; FLH163980.01L; RZPDo839B06161D interleukin 8 (IL8) gene, encodes complete protein.
DQ890564 - Synthetic construct clone IMAGE:100003194; FLH163984.01X; RZPDo839B06162D interleukin 8 (IL8) gene, encodes complete protein.
KJ891460 - Synthetic construct Homo sapiens clone ccsbBroadEn_00854 IL8 gene, encodes complete protein.
Z11686 - H.sapiens mRNA for interleukin 8 (partial).
CR542151 - Homo sapiens full open reading frame cDNA clone RZPDo834G0923D for gene IL8, interleukin 8; complete cds, without stopcodon.
BT007067 - Homo sapiens interleukin 8 mRNA, complete cds.
JD434606 - Sequence 415630 from Patent EP1572962.
JD362945 - Sequence 343969 from Patent EP1572962.
JD093758 - Sequence 74782 from Patent EP1572962.
JD295348 - Sequence 276372 from Patent EP1572962.
JD320330 - Sequence 301354 from Patent EP1572962.
JD381991 - Sequence 363015 from Patent EP1572962.
JD349004 - Sequence 330028 from Patent EP1572962.
JD069062 - Sequence 50086 from Patent EP1572962.
JD334363 - Sequence 315387 from Patent EP1572962.
JD183229 - Sequence 164253 from Patent EP1572962.
AJ227913 - Homo sapiens partial mRNA; ID ED166-8B, EE2-8C.
JD250501 - Sequence 231525 from Patent EP1572962.
JD375523 - Sequence 356547 from Patent EP1572962.
MP584528 - Sequence 27 from Patent WO2020081767.

-  Biochemical and Signaling Pathways
  BioCarta from NCI Cancer Genome Anatomy Project
h_freePathway - Free Radical Induced Apoptosis
h_pepiPathway - Proepithelin Conversion to Epithelin and Wound Repair Control
h_cytokinePathway - Cytokine Network
h_il17Pathway - IL 17 Signaling Pathway
h_inflamPathway - Cytokines and Inflammatory Response
h_granulocytesPathway - Adhesion and Diapedesis of Granulocytes
h_stemPathway - Regulation of hematopoiesis by cytokines
h_LairPathway - Cells and Molecules involved in local acute inflammatory response
h_lympathway - Adhesion and Diapedesis of Lymphocytes
h_nthiPathway - NFkB activation by Nontypeable Hemophilus influenzae

Reactome (by CSHL, EBI, and GO)

Protein P10145 (Reactome details) participates in the following event(s):

R-HSA-374251 DARC is a non-specific receptor for many chemokines
R-HSA-373813 Receptor CXCR2 binds ligands CXCL1 to 7
R-HSA-373791 Receptor CXCR1 binds CXCL6 and CXCL8 ligands
R-HSA-749454 The Ligand:GPCR:Gi complex dissociates
R-HSA-749456 Liganded Gi-activating GPCRs bind inactive heterotrimeric G-protein Gi
R-HSA-380073 Liganded Gi-activating GPCR acts as a GEF for Gi
R-HSA-375276 Peptide ligand-binding receptors
R-HSA-380994 ATF4 activates genes
R-HSA-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-HSA-380108 Chemokine receptors bind chemokines
R-HSA-6783783 Interleukin-10 signaling
R-HSA-6785807 Interleukin-4 and 13 signaling
R-HSA-373076 Class A/1 (Rhodopsin-like receptors)
R-HSA-381042 PERK regulates gene expression
R-HSA-2559583 Cellular Senescence
R-HSA-418594 G alpha (i) signalling events
R-HSA-449147 Signaling by Interleukins
R-HSA-500792 GPCR ligand binding
R-HSA-381119 Unfolded Protein Response (UPR)
R-HSA-2262752 Cellular responses to stress
R-HSA-388396 GPCR downstream signalling
R-HSA-1280215 Cytokine Signaling in Immune system
R-HSA-372790 Signaling by GPCR
R-HSA-392499 Metabolism of proteins
R-HSA-8953897 Cellular responses to external stimuli
R-HSA-168256 Immune System
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: B2R4L8, ENST00000307407.1, ENST00000307407.2, ENST00000307407.3, ENST00000307407.4, ENST00000307407.5, ENST00000307407.6, ENST00000307407.7, IL8, IL8_HUMAN, NM_000584, P10145, Q6FGF6, Q6LAE6, Q96RG6, Q9C077, Q9UCE1, Q9UCR8, Q9UCR9, Q9UCS0, uc317obl.1, uc317obl.2
UCSC ID: ENST00000307407.8_6
RefSeq Accession: NM_000584.4
Protein: P10145 (aka IL8_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.