Human Gene MAP3K12 (ENST00000547488.6_10) from GENCODE V47lift37
  Description: mitogen-activated protein kinase kinase kinase 12, transcript variant 1 (from RefSeq NM_001193511.2)
Gencode Transcript: ENST00000547488.6_10
Gencode Gene: ENSG00000139625.13_13
Transcript (Including UTRs)
   Position: hg19 chr12:53,873,453-53,893,242 Size: 19,790 Total Exon Count: 14 Strand: -
Coding Region
   Position: hg19 chr12:53,874,966-53,881,175 Size: 6,210 Coding Exon Count: 13 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr12:53,873,453-53,893,242)mRNA (may differ from genome)Protein (892 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
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BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: M3K12_HUMAN
DESCRIPTION: RecName: Full=Mitogen-activated protein kinase kinase kinase 12; EC=2.7.11.25; AltName: Full=Dual leucine zipper bearing kinase; Short=DLK; AltName: Full=Leucine-zipper protein kinase; Short=ZPK; AltName: Full=MAPK-upstream kinase; Short=MUK; AltName: Full=Mixed lineage kinase;
FUNCTION: May be an activator of the JNK/SAPK pathway. Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
COFACTOR: Magnesium.
SUBUNIT: Interacts with MBIP.
INTERACTION: Q70Z53:FRA10AC1; NbExp=2; IntAct=EBI-710223, EBI-710176;
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Membrane; Peripheral membrane protein (By similarity).
TISSUE SPECIFICITY: Highly expressed in brain and kidney.
DOMAIN: Interacts with MBIP through the leucine-zipper motif.
PTM: Autophosphorylated on Ser/Thr. Phosphorylated in cytosol under basal conditions and dephosphorylated when membrane- associated (By similarity).
SIMILARITY: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
SIMILARITY: Contains 1 protein kinase domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 71.10 RPKM in Brain - Cerebellum
Total median expression: 668.99 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -24.6069-0.357 Picture PostScript Text
3' UTR -442.901513-0.293 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011009 - Kinase-like_dom
IPR017419 - MAP3K12_MAP3K13
IPR000719 - Prot_kinase_cat_dom
IPR001245 - Ser-Thr/Tyr_kinase_cat_dom
IPR008271 - Ser/Thr_kinase_AS

Pfam Domains:
PF00069 - Protein kinase domain
PF07714 - Protein tyrosine and serine/threonine kinase

SCOP Domains:
56112 - Protein kinase-like (PK-like)

ModBase Predicted Comparative 3D Structure on Q12852
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0004672 protein kinase activity
GO:0004674 protein serine/threonine kinase activity
GO:0004709 MAP kinase kinase kinase activity
GO:0004871 signal transducer activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity
GO:0019901 protein kinase binding
GO:0042803 protein homodimerization activity

Biological Process:
GO:0000165 MAPK cascade
GO:0000186 activation of MAPKK activity
GO:0006468 protein phosphorylation
GO:0007254 JNK cascade
GO:0016310 phosphorylation
GO:0016572 histone phosphorylation
GO:0018105 peptidyl-serine phosphorylation
GO:0018107 peptidyl-threonine phosphorylation
GO:0035556 intracellular signal transduction
GO:0046777 protein autophosphorylation
GO:2000672 negative regulation of motor neuron apoptotic process

Cellular Component:
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0030424 axon
GO:0030426 growth cone


-  Descriptions from all associated GenBank mRNAs
  LF210469 - JP 2014500723-A/17972: Polycomb-Associated Non-Coding RNAs.
LF338132 - JP 2014500723-A/145635: Polycomb-Associated Non-Coding RNAs.
LF338133 - JP 2014500723-A/145636: Polycomb-Associated Non-Coding RNAs.
AB209453 - Homo sapiens mRNA for mitogen-activated protein kinase kinase kinase 12 variant protein.
BC050050 - Homo sapiens mitogen-activated protein kinase kinase kinase 12, mRNA (cDNA clone MGC:42552 IMAGE:4824512), complete cds.
U07358 - Human protein kinase (zpk) mRNA, complete cds.
JD552999 - Sequence 534023 from Patent EP1572962.
JD260864 - Sequence 241888 from Patent EP1572962.
JD380727 - Sequence 361751 from Patent EP1572962.
JD390729 - Sequence 371753 from Patent EP1572962.
JD544830 - Sequence 525854 from Patent EP1572962.
JD504644 - Sequence 485668 from Patent EP1572962.
JD563281 - Sequence 544305 from Patent EP1572962.
JD142593 - Sequence 123617 from Patent EP1572962.
JD450150 - Sequence 431174 from Patent EP1572962.
JD270641 - Sequence 251665 from Patent EP1572962.
JD335972 - Sequence 316996 from Patent EP1572962.
JD530910 - Sequence 511934 from Patent EP1572962.
JD496355 - Sequence 477379 from Patent EP1572962.
JD437885 - Sequence 418909 from Patent EP1572962.
JD342897 - Sequence 323921 from Patent EP1572962.
JD417909 - Sequence 398933 from Patent EP1572962.
KJ904592 - Synthetic construct Homo sapiens clone ccsbBroadEn_13986 MAP3K12-like gene, encodes complete protein.
KJ905341 - Synthetic construct Homo sapiens clone ccsbBroadEn_14884 MAP3K12 gene, encodes complete protein.
AK094195 - Homo sapiens cDNA FLJ36876 fis, clone ASTRO2019171, highly similar to Mitogen-activated protein kinase kinase kinase 12 (EC 2.7.11.25).
JD190968 - Sequence 171992 from Patent EP1572962.
AK314530 - Homo sapiens cDNA, FLJ95351.
LF338134 - JP 2014500723-A/145637: Polycomb-Associated Non-Coding RNAs.
CU690632 - Synthetic construct Homo sapiens gateway clone IMAGE:100020929 5' read MAP3K12 mRNA.
JD026398 - Sequence 7422 from Patent EP1572962.
JD021905 - Sequence 2929 from Patent EP1572962.
JD111162 - Sequence 92186 from Patent EP1572962.
MA446046 - JP 2018138019-A/17972: Polycomb-Associated Non-Coding RNAs.
MA573709 - JP 2018138019-A/145635: Polycomb-Associated Non-Coding RNAs.
MA573710 - JP 2018138019-A/145636: Polycomb-Associated Non-Coding RNAs.
MA573711 - JP 2018138019-A/145637: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  BioCarta from NCI Cancer Genome Anatomy Project
h_mapkPathway - MAPKinase Signaling Pathway

-  Other Names for This Gene
  Alternate Gene Symbols: B3KSS9, ENST00000547488.1, ENST00000547488.2, ENST00000547488.3, ENST00000547488.4, ENST00000547488.5, G3V1Y2, M3K12_HUMAN, NM_001193511, Q12852, Q86VQ5, Q8WY25, uc324ykl.1, uc324ykl.2, ZPK
UCSC ID: ENST00000547488.6_10
RefSeq Accession: NM_001193511.2
Protein: Q12852 (aka M3K12_HUMAN or M3KC_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.