ID:KAP2_HUMAN DESCRIPTION: RecName: Full=cAMP-dependent protein kinase type II-alpha regulatory subunit; FUNCTION: Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase. SUBUNIT: The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Interacts with AKAP4 and CBFA2T3. Interacts with the phosphorylated form of PJA2. Interacts with MYRIP. This interaction may link PKA to components of the exocytosis machinery, thus facilitating exocytosis, including insulin release (By similarity). SUBCELLULAR LOCATION: Cytoplasm. Cell membrane. Note=Co-localizes with PJA2 in the cytoplasm and the cell membrane. TISSUE SPECIFICITY: Four types of regulatory chains are found: I- alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible. PTM: Phosphorylated by the activated catalytic chain. SIMILARITY: Belongs to the cAMP-dependent kinase regulatory chain family. SIMILARITY: Contains 2 cyclic nucleotide-binding domains.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on P13861
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Gene Ontology (GO) Annotations with Structured Vocabulary
Molecular Function: GO:0000166 nucleotide binding GO:0004862 cAMP-dependent protein kinase inhibitor activity GO:0005515 protein binding GO:0008603 cAMP-dependent protein kinase regulator activity GO:0019904 protein domain specific binding GO:0030552 cAMP binding GO:0031625 ubiquitin protein ligase binding GO:0034236 protein kinase A catalytic subunit binding
Biological Process: GO:0001932 regulation of protein phosphorylation GO:0003091 renal water homeostasis GO:0007596 blood coagulation GO:0034199 activation of protein kinase A activity GO:0035556 intracellular signal transduction GO:0071377 cellular response to glucagon stimulus GO:2000480 negative regulation of cAMP-dependent protein kinase activity
AK026351 - Homo sapiens cDNA: FLJ22698 fis, clone HSI12044. DQ579059 - Homo sapiens piRNA piR-47171, complete sequence. AK293042 - Homo sapiens cDNA FLJ75444 complete cds, highly similar to Homo sapiens protein kinase, cAMP-dependent, regulatory, type II, alpha (PRKAR2A), mRNA. AK023535 - Homo sapiens cDNA FLJ13473 fis, clone PLACE1003592. BC002763 - Homo sapiens protein kinase, cAMP-dependent, regulatory, type II, alpha, mRNA (cDNA clone MGC:3606 IMAGE:3629579), complete cds. JD446458 - Sequence 427482 from Patent EP1572962. JD533754 - Sequence 514778 from Patent EP1572962. JD421973 - Sequence 402997 from Patent EP1572962. JD446457 - Sequence 427481 from Patent EP1572962. JD333031 - Sequence 314055 from Patent EP1572962. JD112170 - Sequence 93194 from Patent EP1572962. JD495408 - Sequence 476432 from Patent EP1572962. JD089350 - Sequence 70374 from Patent EP1572962. X14968 - Human testis mRNA for the RII-alpha subunit of cAMP dependent protein kinase. JD101672 - Sequence 82696 from Patent EP1572962. JD551599 - Sequence 532623 from Patent EP1572962. JD114802 - Sequence 95826 from Patent EP1572962. JD257113 - Sequence 238137 from Patent EP1572962. BT007225 - Homo sapiens protein kinase, cAMP-dependent, regulatory, type II, alpha mRNA, complete cds. KJ901660 - Synthetic construct Homo sapiens clone ccsbBroadEn_11054 PRKAR2A gene, encodes complete protein. KJ905285 - Synthetic construct Homo sapiens clone ccsbBroadEn_14786 PRKAR2A gene, encodes complete protein. KU178276 - Homo sapiens protein kinase cAMP-dependent regulatory type II alpha isoform 1 (PRKAR2A) mRNA, partial cds, alternatively spliced. KU178277 - Homo sapiens protein kinase cAMP-dependent regulatory type II alpha isoform 2 (PRKAR2A) mRNA, partial cds. AB464095 - Synthetic construct DNA, clone: pF1KB6582, Homo sapiens PRKAR2A gene for protein kinase, cAMP-dependent, regulatory, type II alpha, without stop codon, in Flexi system. CU678176 - Synthetic construct Homo sapiens gateway clone IMAGE:100019171 5' read PRKAR2A mRNA. GQ472202 - Homo sapiens epididymis secretory sperm binding protein Li 169mP (HEL-S-169mP) mRNA, complete cds. JD458592 - Sequence 439616 from Patent EP1572962. JD204692 - Sequence 185716 from Patent EP1572962. JD458596 - Sequence 439620 from Patent EP1572962.
Biochemical and Signaling Pathways
BioCarta from NCI Cancer Genome Anatomy Project h_CSKPathway - Activation of Csk by cAMP-dependent Protein Kinase Inhibits Signaling through the T Cell Receptor h_mCalpainPathway - mCalpain and friends in Cell motility h_nos1Pathway - Nitric Oxide Signaling Pathway h_badPathway - Regulation of BAD phosphorylation h_vipPathway - Neuropeptides VIP and PACAP inhibit the apoptosis of activated T cells h_cftrPathway - Cystic fibrosis transmembrane conductance regulator (CFTR) and beta 2 adrenergic receptor (b2AR) pathway h_crebPathway - Transcription factor CREB and its extracellular signals h_dreampathway - Repression of Pain Sensation by the Transcriptional Regulator DREAM h_igf1rPathway - Multiple antiapoptotic pathways from IGF-1R signaling lead to BAD phosphorylation h_mPRPathway - How Progesterone Initiates the Oocyte Maturation h_nfatPathway - NFAT and Hypertrophy of the heart (Transcription in the broken heart) h_gpcrPathway - Signaling Pathway from G-Protein Families h_plcePathway - Phospholipase C-epsilon pathway h_agpcrPathway - Attenuation of GPCR Signaling h_chrebpPathway - ChREBP regulation by carbohydrates and cAMP h_ck1Pathway - Regulation of ck1/cdk5 by type 1 glutamate receptors h_pparaPathway - Mechanism of Gene Regulation by Peroxisome Proliferators via PPARa(alpha) h_akap95Pathway - AKAP95 role in mitosis and chromosome dynamics h_GATA3pathway - GATA3 participate in activating the Th2 cytokine genes expression h_carm1Pathway - Transcription Regulation by Methyltransferase of CARM1 h_no1Pathway - Actions of Nitric Oxide in the Heart h_shhPathway - Sonic Hedgehog (Shh) Pathway h_stathminPathway - Stathmin and breast cancer resistance to antimicrotubule agents h_akap13Pathway - Rho-Selective Guanine Exchange Factor AKAP13 Mediates Stress Fiber Formation h_akapCentrosomePathway - Protein Kinase A at the Centrosome
Reactome (by CSHL, EBI, and GO)
Protein P13861 (Reactome details) participates in the following event(s):
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
