Human Gene TRIOBP (ENST00000644935.1_8) from GENCODE V47lift37
  Description: TRIO and F-actin binding protein, transcript variant 6 (from RefSeq NM_001039141.3)
Gencode Transcript: ENST00000644935.1_8
Gencode Gene: ENSG00000100106.22_19
Transcript (Including UTRs)
   Position: hg19 chr22:38,093,055-38,172,563 Size: 79,509 Total Exon Count: 24 Strand: +
Coding Region
   Position: hg19 chr22:38,097,373-38,168,769 Size: 71,397 Coding Exon Count: 21 

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Gene AllelesMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesGeneReviewsModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr22:38,093,055-38,172,563)mRNA (may differ from genome)Protein (2365 aa)
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-  Comments and Description Text from UniProtKB
  ID: TARA_HUMAN
DESCRIPTION: RecName: Full=TRIO and F-actin-binding protein; AltName: Full=Protein Tara; AltName: Full=Trio-associated repeat on actin;
FUNCTION: May regulate actin cytoskeletal organization, cell spreading and cell contraction by directly binding and stabilizing filamentous F-actin. The localized formation of TARA and TRIO complexes coordinates the amount of F-actin present in stress fibers. May also serve as a linker protein to recruit proteins required for F-actin formation and turnover.
SUBUNIT: Binds to TRIO and F-actin. May also interact with myosin II. Interacts with HECTD3.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm, cytoskeleton. Note=Localized to F-actin in a periodic pattern.
TISSUE SPECIFICITY: Widely expressed. Highly expressed in heart and placenta. Isoform 3 is expressed in fetal brain, retina and cochlea but is not detectable in the other tissues.
DOMAIN: Contains at least 2 actin-binding sites per coiled-coil dimer.
PTM: Ubiquitinated by HECTD3, leading to its degradation by the proteasome.
DISEASE: Defects in TRIOBP are the cause of deafness autosomal recessive type 28 (DFNB28) [MIM:609823].
MISCELLANEOUS: Has been identified in PubMed:9853615 by a selection system for genes encoding nuclear-targeted protein.
SIMILARITY: Contains 1 PH domain.
SEQUENCE CAUTION: Sequence=BAA34800.1; Type=Frameshift; Positions=478;

-  Primer design for this transcript
 

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Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: TRIOBP
Diseases sorted by gene-association score: deafness, autosomal recessive 28* (1238), nonsyndromic deafness* (161), nonsyndromic hearing loss and deafness* (133), dfnb28 nonsyndromic hearing loss and deafness* (100), autosomal recessive non-syndromic sensorineural deafness type dfnb* (76), autosomal recessive nonsyndromic deafness (19), deafness, autosomal recessive 37 (12)
* = Manually curated disease association

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
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+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -71.40211-0.338 Picture PostScript Text
3' UTR -1058.602776-0.381 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011993 - PH_like_dom
IPR001849 - Pleckstrin_homology

Pfam Domains:
PF00169 - PH domain

SCOP Domains:
50729 - PH domain-like

ModBase Predicted Comparative 3D Structure on Q9H2D6
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003779 actin binding
GO:0017049 GTP-Rho binding
GO:0031625 ubiquitin protein ligase binding
GO:0045159 myosin II binding
GO:0051015 actin filament binding

Biological Process:
GO:0007049 cell cycle
GO:0007605 sensory perception of sound
GO:0030047 actin modification
GO:0032956 regulation of actin cytoskeleton organization
GO:0051016 barbed-end actin filament capping
GO:0051017 actin filament bundle assembly
GO:0051301 cell division
GO:0060088 auditory receptor cell stereocilium organization
GO:1900026 positive regulation of substrate adhesion-dependent cell spreading

Cellular Component:
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005815 microtubule organizing center
GO:0005856 cytoskeleton
GO:0005925 focal adhesion
GO:0015629 actin cytoskeleton
GO:0030496 midbody
GO:0032420 stereocilium
GO:0032437 cuticular plate


-  Descriptions from all associated GenBank mRNAs
  KX533494 - Homo sapiens TRIO and F-actin-binding protein (TRIOBP) mRNA, complete cds.
DQ278603 - Homo sapiens trio-associated repeat on actin (TRIOBP) mRNA, complete cds.
AK096634 - Homo sapiens cDNA FLJ39315 fis, A-OCBBF2013926.
DQ228003 - Homo sapiens TRIOBP isoform 3 (TRIOBP) mRNA, complete cds.
DQ228005 - Homo sapiens TRIOBP isoform 6 (TRIOBP) mRNA, complete cds.
AB587318 - Synthetic construct DNA, clone: pF1KA1662, Homo sapiens TRIOBP gene for TRIO and F-actin binding protein, without stop codon, in Flexi system.
AB051449 - Homo sapiens mRNA for KIAA1662 protein, partial cds.
DQ228004 - Homo sapiens TRIOBP isoform 4 (TRIOBP) mRNA, complete cds.
BC022200 - Homo sapiens TRIO and F-actin binding protein, mRNA (cDNA clone IMAGE:3356367).
AF281030 - Homo sapiens Tara mRNA, complete cds.
BC003618 - Homo sapiens TRIO and F-actin binding protein, mRNA (cDNA clone IMAGE:2988379), complete cds.
AM392748 - Synthetic construct Homo sapiens clone IMAGE:100002693 for hypothetical protein (TRIOBP gene).
AM392798 - Synthetic construct Homo sapiens clone IMAGE:100002694 for hypothetical protein (TRIOBP gene).
BC004303 - Homo sapiens TRIO and F-actin binding protein, mRNA (cDNA clone IMAGE:3623592), partial cds.
BC013278 - Homo sapiens TRIO and F-actin binding protein, mRNA (cDNA clone IMAGE:3639826), partial cds.
AL713698 - Homo sapiens mRNA; cDNA DKFZp547M048 (from clone DKFZp547M048).
AB015343 - Homo sapiens HRIHFB2122 mRNA, partial cds.
JD405594 - Sequence 386618 from Patent EP1572962.
JD177904 - Sequence 158928 from Patent EP1572962.
JD334220 - Sequence 315244 from Patent EP1572962.
JD098000 - Sequence 79024 from Patent EP1572962.
JD103042 - Sequence 84066 from Patent EP1572962.
JD052049 - Sequence 33073 from Patent EP1572962.
JD240320 - Sequence 221344 from Patent EP1572962.
JD139714 - Sequence 120738 from Patent EP1572962.
JD524487 - Sequence 505511 from Patent EP1572962.
JD297349 - Sequence 278373 from Patent EP1572962.
JD527755 - Sequence 508779 from Patent EP1572962.
JD470698 - Sequence 451722 from Patent EP1572962.
JD111958 - Sequence 92982 from Patent EP1572962.
JD337270 - Sequence 318294 from Patent EP1572962.
JD070630 - Sequence 51654 from Patent EP1572962.
JD342528 - Sequence 323552 from Patent EP1572962.
JD046081 - Sequence 27105 from Patent EP1572962.
JD154033 - Sequence 135057 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: B1AHD4, B1AHD7, F2Z2W0, F8W6V6, HRIHFB2122, KIAA1662, NM_001039141, O94797, Q2PZW8, Q2Q3Z9, Q2Q400, Q5R3M6, Q96DW1, Q9BT77, Q9BTL7, Q9BY98, Q9H2D6, Q9Y3L4, TARA , TARA_HUMAN, uc328lcj.1, uc328lcj.2
UCSC ID: ENST00000644935.1_8
RefSeq Accession: NM_001039141.3
Protein: Q9H2D6 (aka TARA_HUMAN)

-  GeneReviews for This Gene
  GeneReviews article(s) related to gene TRIOBP:
deafness-overview (Genetic Hearing Loss Overview)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.