Human Gene ADORA2B (ENST00000304222.3_3) from GENCODE V47lift37
  Description: adenosine A2b receptor (from RefSeq NM_000676.4)
Gencode Transcript: ENST00000304222.3_3
Gencode Gene: ENSG00000170425.4_6
Transcript (Including UTRs)
   Position: hg19 chr17:15,848,444-15,879,060 Size: 30,617 Total Exon Count: 2 Strand: +
Coding Region
   Position: hg19 chr17:15,848,563-15,878,656 Size: 30,094 Coding Exon Count: 2 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr17:15,848,444-15,879,060)mRNA (may differ from genome)Protein (332 aa)
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HGNCMGIOMIMPubMedReactomeUniProtKB
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-  Comments and Description Text from UniProtKB
  ID: AA2BR_HUMAN
DESCRIPTION: RecName: Full=Adenosine receptor A2b;
FUNCTION: Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
SIMILARITY: Belongs to the G-protein coupled receptor 1 family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene
  • D013749 Tetrachlorodibenzodioxin
  • D000241 Adenosine
  • D008070 Lipopolysaccharides
  • C049325 1,2-dithiol-3-thione
  • C021183 1,7-dimethylxanthine
  • C111118 2',3,3',4',5-pentachloro-4-hydroxybiphenyl
  • C036990 2-amino-3,8-dimethylimidazo(4,5-f)quinoxaline
  • C027576 4-hydroxy-2-nonenal
  • D015123 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide
  • D019830 Adenosine-5'-(N-ethylcarboxamide)
          more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 7.20 RPKM in Colon - Transverse
Total median expression: 98.25 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -69.40119-0.583 Picture PostScript Text
3' UTR -89.80404-0.222 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000276 - 7TM_GPCR_Rhodpsn
IPR001435 - Adeno_A2B_rcpt
IPR001634 - Adenosn_rcpt
IPR017452 - GPCR_Rhodpsn_supfam

Pfam Domains:
PF00001 - 7 transmembrane receptor (rhodopsin family)
PF10320 - Serpentine type 7TM GPCR chemoreceptor Srsx
PF13853 - Olfactory receptor

SCOP Domains:
81321 - Family A G protein-coupled receptor-like

ModBase Predicted Comparative 3D Structure on P29275
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0001609 G-protein coupled adenosine receptor activity
GO:0004871 signal transducer activity
GO:0004930 G-protein coupled receptor activity

Biological Process:
GO:0000187 activation of MAPK activity
GO:0001973 adenosine receptor signaling pathway
GO:0002882 positive regulation of chronic inflammatory response to non-antigenic stimulus
GO:0006968 cellular defense response
GO:0007165 signal transduction
GO:0007186 G-protein coupled receptor signaling pathway
GO:0007189 adenylate cyclase-activating G-protein coupled receptor signaling pathway
GO:0007190 activation of adenylate cyclase activity
GO:0007254 JNK cascade
GO:0007588 excretion
GO:0010575 positive regulation of vascular endothelial growth factor production
GO:0030828 positive regulation of cGMP biosynthetic process
GO:0031284 positive regulation of guanylate cyclase activity
GO:0031668 cellular response to extracellular stimulus
GO:0032722 positive regulation of chemokine production
GO:0032755 positive regulation of interleukin-6 production
GO:0043306 positive regulation of mast cell degranulation
GO:0044267 cellular protein metabolic process
GO:0060087 relaxation of vascular smooth muscle

Cellular Component:
GO:0005622 intracellular
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  X68487 - H.sapiens mRNA for A2b adenosine receptor.
M97759 - Human adenosine A2b receptor (ADORA2) mRNA, complete cds.
BC025722 - Homo sapiens adenosine A2b receptor, mRNA (cDNA clone MGC:34640 IMAGE:5198898), complete cds.
AB464451 - Synthetic construct DNA, clone: pF1KB8955, Homo sapiens ADORA2B gene for adenosine A2b receptor, without stop codon, in Flexi system.
DQ891714 - Synthetic construct clone IMAGE:100004344; FLH179348.01X; RZPDo839F06130D adenosine A2b receptor (ADORA2B) gene, encodes complete protein.
DQ894891 - Synthetic construct Homo sapiens clone IMAGE:100009351; FLH179344.01L; RZPDo839F06129D adenosine A2b receptor (ADORA2B) gene, encodes complete protein.
CU693036 - Synthetic construct Homo sapiens gateway clone IMAGE:100016719 5' read ADORA2B mRNA.
KJ890636 - Synthetic construct Homo sapiens clone ccsbBroadEn_00030 ADORA2B gene, encodes complete protein.
AY136748 - Homo sapiens adenosine receptor A2B (ADORA2B) mRNA, complete cds.
JD062484 - Sequence 43508 from Patent EP1572962.
JD437025 - Sequence 418049 from Patent EP1572962.
AK172851 - Homo sapiens cDNA FLJ24012 fis, clone LNG11380, highly similar to Adenosine A2b receptor.
JD070958 - Sequence 51982 from Patent EP1572962.
JD203096 - Sequence 184120 from Patent EP1572962.
JD239479 - Sequence 220503 from Patent EP1572962.
JD478724 - Sequence 459748 from Patent EP1572962.
JD551539 - Sequence 532563 from Patent EP1572962.
JD305752 - Sequence 286776 from Patent EP1572962.
JD357095 - Sequence 338119 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein P29275 (Reactome details) participates in the following event(s):

R-HSA-418925 ADORA2A,B bind Ade-Rib
R-HSA-744886 The Ligand:GPCR:Gs complex dissociates
R-HSA-744887 Liganded Gs-activating GPCRs bind inactive heterotrimeric Gs
R-HSA-379044 Liganded Gs-activating GPCR acts as a GEF for Gs
R-HSA-417973 Adenosine P1 receptors
R-HSA-5683826 Surfactant metabolism
R-HSA-418555 G alpha (s) signalling events
R-HSA-418038 Nucleotide-like (purinergic) receptors
R-HSA-392499 Metabolism of proteins
R-HSA-388396 GPCR downstream signalling
R-HSA-373076 Class A/1 (Rhodopsin-like receptors)
R-HSA-372790 Signaling by GPCR
R-HSA-500792 GPCR ligand binding
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: AA2BR_HUMAN, ENST00000304222.1, ENST00000304222.2, NM_000676, P29275, uc317njp.1, uc317njp.2
UCSC ID: ENST00000304222.3_3
RefSeq Accession: NM_000676.4
Protein: P29275 (aka AA2BR_HUMAN or AA2B_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.