Human Gene AGO1 (ENST00000373204.6_4) from GENCODE V47lift37
  Description: argonaute RISC component 1, transcript variant 1 (from RefSeq NM_012199.5)
Gencode Transcript: ENST00000373204.6_4
Gencode Gene: ENSG00000092847.14_13
Transcript (Including UTRs)
   Position: hg19 chr1:36,348,810-36,396,133 Size: 47,324 Total Exon Count: 19 Strand: +
Coding Region
   Position: hg19 chr1:36,349,023-36,385,208 Size: 36,186 Coding Exon Count: 19 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:36,348,810-36,396,133)mRNA (may differ from genome)Protein (857 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: AGO1_HUMAN
DESCRIPTION: RecName: Full=Protein argonaute-1; Short=Argonaute1; Short=hAgo1; AltName: Full=Eukaryotic translation initiation factor 2C 1; Short=eIF-2C 1; Short=eIF2C 1; AltName: Full=Putative RNA-binding protein Q99;
FUNCTION: Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs).
SUBUNIT: Interacts with DDB1, DDX5, DDX6, DHX30, DHX36, DDX47, DICER1, EIF2C2/AGO2, ELAVL1, HNRNPF, IGF2BP1, ILF3, IMP8, MATR3, MOV10, PABPC1, PRMT5, RBM4, SART3, TNRC6B, UPF1 and YBX1. Associates with polysomes and messenger ribonucleoproteins (mNRPs). Interacts with LIMD1, WTIP and AJUBA.
INTERACTION: Q9UPY3:DICER1; NbExp=2; IntAct=EBI-527363, EBI-395506; O15397:IPO8; NbExp=2; IntAct=EBI-527363, EBI-358808;
SUBCELLULAR LOCATION: Cytoplasm, P-body.
SIMILARITY: Belongs to the argonaute family. Ago subfamily.
SIMILARITY: Contains 1 PAZ domain.
SIMILARITY: Contains 1 Piwi domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: AGO1
Diseases sorted by gene-association score: fungal meningitis (5)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 7.48 RPKM in Brain - Cerebellar Hemisphere
Total median expression: 215.10 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -123.40213-0.579 Picture PostScript Text
3' UTR -3422.4010925-0.313 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR014811 - DUF1785
IPR003100 - PAZ
IPR003165 - Piwi
IPR012337 - RNaseH-like_dom

Pfam Domains:
PF02170 - PAZ domain
PF02171 - Piwi domain
PF08699 - Argonaute linker 1 domain
PF16486 - N-terminal domain of argonaute
PF16487 - Mid domain of argonaute
PF16488 - Argonaute linker 2 domain

SCOP Domains:
101690 - PAZ domain
53098 - Ribonuclease H-like

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1SI2 - X-ray MuPIT 1SI3 - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q9UL18
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGD    
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000978 RNA polymerase II core promoter proximal region sequence-specific DNA binding
GO:0000993 RNA polymerase II core binding
GO:0001047 core promoter binding
GO:0003676 nucleic acid binding
GO:0003723 RNA binding
GO:0003725 double-stranded RNA binding
GO:0003727 single-stranded RNA binding
GO:0005515 protein binding
GO:0035198 miRNA binding
GO:0044212 transcription regulatory region DNA binding
GO:0004521 endoribonuclease activity

Biological Process:
GO:0000956 nuclear-transcribed mRNA catabolic process
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006417 regulation of translation
GO:0007223 Wnt signaling pathway, calcium modulating pathway
GO:0010501 RNA secondary structure unwinding
GO:0010586 miRNA metabolic process
GO:0010628 positive regulation of gene expression
GO:0016525 negative regulation of angiogenesis
GO:0031047 gene silencing by RNA
GO:0031054 pre-miRNA processing
GO:0035194 posttranscriptional gene silencing by RNA
GO:0035196 production of miRNAs involved in gene silencing by miRNA
GO:0035278 miRNA mediated inhibition of translation
GO:0035280 miRNA loading onto RISC involved in gene silencing by miRNA
GO:0045652 regulation of megakaryocyte differentiation
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0060964 regulation of gene silencing by miRNA
GO:1901224 positive regulation of NIK/NF-kappaB signaling
GO:0035279 mRNA cleavage involved in gene silencing by miRNA
GO:0090625 mRNA cleavage involved in gene silencing by siRNA

Cellular Component:
GO:0000932 P-body
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005844 polysome
GO:0016442 RISC complex
GO:0035068 micro-ribonucleoprotein complex
GO:0070578 RISC-loading complex
GO:1990904 ribonucleoprotein complex


-  Descriptions from all associated GenBank mRNAs
  AK001622 - Homo sapiens cDNA FLJ10760 fis, clone NT2RP3004618, highly similar to Eukaryotic translation initiation factor 2C 1.
JD060486 - Sequence 41510 from Patent EP1572962.
AF093097 - Homo sapiens putative RNA-binding protein Q99 mRNA, complete cds.
BC063275 - Homo sapiens eukaryotic translation initiation factor 2C, 1, mRNA (cDNA clone MGC:71487 IMAGE:30344513), complete cds.
AK314148 - Homo sapiens cDNA, FLJ94852, highly similar to Homo sapiens eukaryotic translation initiation factor 2C, 1 (EIF2C1), mRNA.
JD409007 - Sequence 390031 from Patent EP1572962.
JD484920 - Sequence 465944 from Patent EP1572962.
JD443987 - Sequence 425011 from Patent EP1572962.
KJ898629 - Synthetic construct Homo sapiens clone ccsbBroadEn_08023 EIF2C1 gene, encodes complete protein.
AB385028 - Synthetic construct DNA, clone: pF1KB5078, Homo sapiens EIF2C1 gene for eukaryotic translation initiation factor 2C 1, complete cds, without stop codon, in Flexi system.
CR936823 - Homo sapiens mRNA; cDNA DKFZp686M13167 (from clone DKFZp686M13167).
DQ595629 - Homo sapiens piRNA piR-61741, complete sequence.
AK002003 - Homo sapiens cDNA FLJ11141 fis, clone PLACE1006531.
JD380716 - Sequence 361740 from Patent EP1572962.
JD304876 - Sequence 285900 from Patent EP1572962.
JD516890 - Sequence 497914 from Patent EP1572962.
JD309419 - Sequence 290443 from Patent EP1572962.
JD340316 - Sequence 321340 from Patent EP1572962.
JD481896 - Sequence 462920 from Patent EP1572962.
JD236883 - Sequence 217907 from Patent EP1572962.
JD390374 - Sequence 371398 from Patent EP1572962.
JD052497 - Sequence 33521 from Patent EP1572962.
JD084450 - Sequence 65474 from Patent EP1572962.
JD184506 - Sequence 165530 from Patent EP1572962.
AK027611 - Homo sapiens cDNA FLJ14705 fis, clone NT2RP3000562, highly similar to Homo sapiens putative RNA-binding protein Q99 mRNA.
JD124455 - Sequence 105479 from Patent EP1572962.
JD493231 - Sequence 474255 from Patent EP1572962.
JD438547 - Sequence 419571 from Patent EP1572962.
JD237784 - Sequence 218808 from Patent EP1572962.
JD185983 - Sequence 167007 from Patent EP1572962.
JD262969 - Sequence 243993 from Patent EP1572962.
JD218923 - Sequence 199947 from Patent EP1572962.
JD220541 - Sequence 201565 from Patent EP1572962.
JD340423 - Sequence 321447 from Patent EP1572962.
JD382226 - Sequence 363250 from Patent EP1572962.
JD434872 - Sequence 415896 from Patent EP1572962.
JD359527 - Sequence 340551 from Patent EP1572962.
JD120109 - Sequence 101133 from Patent EP1572962.
JD087788 - Sequence 68812 from Patent EP1572962.
JD288940 - Sequence 269964 from Patent EP1572962.
JD165361 - Sequence 146385 from Patent EP1572962.
JD518508 - Sequence 499532 from Patent EP1572962.
JD323747 - Sequence 304771 from Patent EP1572962.
JD467113 - Sequence 448137 from Patent EP1572962.
JD229806 - Sequence 210830 from Patent EP1572962.
JD297977 - Sequence 279001 from Patent EP1572962.
JD547433 - Sequence 528457 from Patent EP1572962.
JD214826 - Sequence 195850 from Patent EP1572962.
JD359550 - Sequence 340574 from Patent EP1572962.
JD430817 - Sequence 411841 from Patent EP1572962.
JD543904 - Sequence 524928 from Patent EP1572962.
JD494536 - Sequence 475560 from Patent EP1572962.
JD262884 - Sequence 243908 from Patent EP1572962.
JD339850 - Sequence 320874 from Patent EP1572962.
JD329517 - Sequence 310541 from Patent EP1572962.
JD172813 - Sequence 153837 from Patent EP1572962.
JD435236 - Sequence 416260 from Patent EP1572962.
JD281057 - Sequence 262081 from Patent EP1572962.
JD146996 - Sequence 128020 from Patent EP1572962.
JD171954 - Sequence 152978 from Patent EP1572962.
JD526940 - Sequence 507964 from Patent EP1572962.
JD074797 - Sequence 55821 from Patent EP1572962.
JD474860 - Sequence 455884 from Patent EP1572962.
JD077175 - Sequence 58199 from Patent EP1572962.
JD348345 - Sequence 329369 from Patent EP1572962.
JD525967 - Sequence 506991 from Patent EP1572962.
JD319148 - Sequence 300172 from Patent EP1572962.
JD275119 - Sequence 256143 from Patent EP1572962.
JD500775 - Sequence 481799 from Patent EP1572962.
JD259230 - Sequence 240254 from Patent EP1572962.
JD295197 - Sequence 276221 from Patent EP1572962.
JD082148 - Sequence 63172 from Patent EP1572962.
AK000326 - Homo sapiens cDNA FLJ20319 fis, clone HEP08731.
DQ595010 - Homo sapiens piRNA piR-61122, complete sequence.
JD215107 - Sequence 196131 from Patent EP1572962.
JD521987 - Sequence 503011 from Patent EP1572962.
JD201658 - Sequence 182682 from Patent EP1572962.
JD458960 - Sequence 439984 from Patent EP1572962.
JD193953 - Sequence 174977 from Patent EP1572962.
JD422804 - Sequence 403828 from Patent EP1572962.
JD193954 - Sequence 174978 from Patent EP1572962.
JD422805 - Sequence 403829 from Patent EP1572962.
JD193955 - Sequence 174979 from Patent EP1572962.
JD193956 - Sequence 174980 from Patent EP1572962.
JD193959 - Sequence 174983 from Patent EP1572962.
JD193961 - Sequence 174985 from Patent EP1572962.
DQ577919 - Homo sapiens piRNA piR-46031, complete sequence.
JD135054 - Sequence 116078 from Patent EP1572962.
JD135055 - Sequence 116079 from Patent EP1572962.
JD473129 - Sequence 454153 from Patent EP1572962.
JD378969 - Sequence 359993 from Patent EP1572962.
JD072573 - Sequence 53597 from Patent EP1572962.
JD154384 - Sequence 135408 from Patent EP1572962.
JD171192 - Sequence 152216 from Patent EP1572962.
JD502132 - Sequence 483156 from Patent EP1572962.
JD343979 - Sequence 325003 from Patent EP1572962.
JD206541 - Sequence 187565 from Patent EP1572962.
JD298713 - Sequence 279737 from Patent EP1572962.
JD480576 - Sequence 461600 from Patent EP1572962.
U92986 - Homo sapiens clone DT1P1C6 mRNA, CAG repeat region.
JD343395 - Sequence 324419 from Patent EP1572962.
JD066744 - Sequence 47768 from Patent EP1572962.
JD046637 - Sequence 27661 from Patent EP1572962.
JD324597 - Sequence 305621 from Patent EP1572962.
JD251003 - Sequence 232027 from Patent EP1572962.
JD380449 - Sequence 361473 from Patent EP1572962.
JD167869 - Sequence 148893 from Patent EP1572962.
JD508061 - Sequence 489085 from Patent EP1572962.
JD068318 - Sequence 49342 from Patent EP1572962.
JD236398 - Sequence 217422 from Patent EP1572962.
JD447899 - Sequence 428923 from Patent EP1572962.
JD252357 - Sequence 233381 from Patent EP1572962.
LF206617 - JP 2014500723-A/14120: Polycomb-Associated Non-Coding RNAs.
AK125879 - Homo sapiens cDNA FLJ43891 fis, clone TESTI4009454.
DQ571915 - Homo sapiens piRNA piR-40027, complete sequence.
AK025726 - Homo sapiens cDNA: FLJ22073 fis, clone HEP11868.
JD070579 - Sequence 51603 from Patent EP1572962.
JD203764 - Sequence 184788 from Patent EP1572962.
JD505558 - Sequence 486582 from Patent EP1572962.
LF356595 - JP 2014500723-A/164098: Polycomb-Associated Non-Coding RNAs.
LF356594 - JP 2014500723-A/164097: Polycomb-Associated Non-Coding RNAs.
MA442194 - JP 2018138019-A/14120: Polycomb-Associated Non-Coding RNAs.
MA592172 - JP 2018138019-A/164098: Polycomb-Associated Non-Coding RNAs.
MA592171 - JP 2018138019-A/164097: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  BioCarta from NCI Cancer Genome Anatomy Project
h_dicerPathway - Dicer Pathway

Reactome (by CSHL, EBI, and GO)

Protein Q9UL18 (Reactome details) participates in the following event(s):

R-HSA-426522 Nonendonucleolytic RISC binds exactly matching target RNAs
R-HSA-5578742 AGO1,2:small RNA complexes interact with chromatin
R-HSA-426489 RISC binds inexactly matching target RNAs
R-HSA-3209151 miR-24 binds p16-INK4a and p14-ARF mRNAs
R-HSA-8935766 miR-378 binds RUNX1 mRNA
R-HSA-8937134 miR-27a binds RUNX1 mRNA
R-HSA-8938440 miR-17 binds RUNX1 mRNA
R-HSA-8938487 miR-20a binds RUNX1 mRNA
R-HSA-8938507 miR-106a binds RUNX1 mRNA
R-HSA-8944650 miR-214 microRNA binds PTEN mRNA
R-HSA-8944706 miR-21 nonendonucleolytic RISC binds PTEN mRNA
R-HSA-203862 Dicer cleaves pre-miRNA to yield duplex miRNA
R-HSA-426464 Dicer cleaves double-stranded RNA to yield double-stranded siRNA
R-HSA-1606561 MIR449 microRNAs bind 3'UTR of NOTCH1 mRNA
R-HSA-1606682 MIR34 microRNAs bind 3'UTR of NOTCH1 mRNA
R-HSA-1912367 MIR34 microRNAs bind 3'UTR of NOTCH2 mRNA
R-HSA-1912363 MIR200B/C microRNAs bind NOTCH1 mRNA
R-HSA-1912366 MIR206 microRNA binds 3'UTR of NOTCH3 mRNA
R-HSA-1912368 MIR302A microRNA binds 3'UTR of NOTCH4 mRNA
R-HSA-1912364 MIR181C microRNA binds 3'UTR of NOTCH4 mRNA
R-HSA-1912362 MIR150 microRNA binds 3'UTR of NOTCH3 mRNA
R-HSA-2318752 miR-26A microRNAs bind PTEN mRNA
R-HSA-8935864 miR-302b binds RUNX1 mRNA
R-HSA-8935930 miR-18a binds RUNX1 mRNA
R-HSA-8936058 miR-675 binds RUNX1 mRNA
R-HSA-8944483 miR-17 microRNA binds PTEN mRNA
R-HSA-8948582 miR-17 microRNA binds VAPA mRNA
R-HSA-8948583 miR-17 microRNA binds CNOT6L mRNA
R-HSA-8939129 miR-215 binds RUNX1 mRNA
R-HSA-8948524 PTENP1 mRNA binds miR-19b RISC
R-HSA-8948569 miR-19b microRNA binds PTEN mRNA
R-HSA-8948612 miR-19b microRNA binds CNOT6L mRNA
R-HSA-8944538 miR-22 microRNA binds PTEN mRNA
R-HSA-8944569 miR-25 microRNA binds PTEN mRNA
R-HSA-8944599 miR-93 microRNA binds PTEN mRNA
R-HSA-8944632 miR-106 microRNAs bind PTEN mRNA
R-HSA-8948641 miR-106 microRNAs bind VAPA mRNA
R-HSA-8948651 miR-106a,(miR106b) microRNA binds CNOT6L mRNA
R-HSA-8944684 miR-205 microRNA binds PTEN mRNA
R-HSA-8945709 miR-20 microRNAs bind PTEN mRNA
R-HSA-8948536 PTENP1 mRNA binds miR-20 RISC
R-HSA-8948621 miR-20 microRNAs bind VAPA mRNA
R-HSA-8948623 miR-20 microRNAs bind CNOT6L mRNA
R-HSA-8944522 miR-19a microRNA binds PTEN mRNA
R-HSA-8948594 miR-19a microRNA binds VAPA mRNA
R-HSA-8948602 miR-19a microRNA binds CNOT6L mRNA
R-HSA-9011958 miR-26A and B bind to the 3'UTR of the GREB1 mRNA
R-HSA-9012203 miR-26A and B bind to the 3'UTR of the CHD11 mRNA
R-HSA-9012208 miR-26A and B bind to the 3'UTR of the KPNA2 mRNA
R-HSA-426496 Post-transcriptional silencing by small RNAs
R-HSA-5578749 Transcriptional regulation by small RNAs
R-HSA-211000 Gene Silencing by RNA
R-HSA-2559580 Oxidative Stress Induced Senescence
R-HSA-2559585 Oncogene Induced Senescence
R-HSA-8934593 Regulation of RUNX1 Expression and Activity
R-HSA-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function
R-HSA-8943723 Regulation of PTEN mRNA translation
R-HSA-203927 MicroRNA (miRNA) biogenesis
R-HSA-426486 Small interfering RNA (siRNA) biogenesis
R-HSA-74160 Gene expression (Transcription)
R-HSA-1912408 Pre-NOTCH Transcription and Translation
R-HSA-5628897 TP53 Regulates Metabolic Genes
R-HSA-2559583 Cellular Senescence
R-HSA-4086398 Ca2+ pathway
R-HSA-5687128 MAPK6/MAPK4 signaling
R-HSA-8878171 Transcriptional regulation by RUNX1
R-HSA-8948700 Competing endogenous RNAs (ceRNAs) regulate PTEN translation
R-HSA-6807070 PTEN Regulation
R-HSA-1912422 Pre-NOTCH Expression and Processing
R-HSA-9018519 Estrogen-dependent gene expression
R-HSA-3700989 Transcriptional Regulation by TP53
R-HSA-2262752 Cellular responses to stress
R-HSA-3858494 Beta-catenin independent WNT signaling
R-HSA-5683057 MAPK family signaling cascades
R-HSA-212436 Generic Transcription Pathway
R-HSA-1257604 PIP3 activates AKT signaling
R-HSA-157118 Signaling by NOTCH
R-HSA-8939211 ESR-mediated signaling
R-HSA-8953897 Cellular responses to external stimuli
R-HSA-195721 Signaling by WNT
R-HSA-162582 Signal Transduction
R-HSA-73857 RNA Polymerase II Transcription
R-HSA-9006925 Intracellular signaling by second messengers
R-HSA-9006931 Signaling by Nuclear Receptors

-  Other Names for This Gene
  Alternate Gene Symbols: AGO1_HUMAN, EIF2C1, ENST00000373204.1, ENST00000373204.2, ENST00000373204.3, ENST00000373204.4, ENST00000373204.5, NM_012199, Q5TA57, Q6P4S0, Q9UL18, uc318kos.1, uc318kos.2
UCSC ID: ENST00000373204.6_4
RefSeq Accession: NM_001317122.2
Protein: Q9UL18 (aka AGO1_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.