Human Gene AGPAT5 (ENST00000285518.11_5) from GENCODE V47lift37
  Description: 1-acylglycerol-3-phosphate O-acyltransferase 5 (from RefSeq NM_018361.5)
Gencode Transcript: ENST00000285518.11_5
Gencode Gene: ENSG00000155189.12_9
Transcript (Including UTRs)
   Position: hg19 chr8:6,566,163-6,619,024 Size: 52,862 Total Exon Count: 8 Strand: +
Coding Region
   Position: hg19 chr8:6,566,190-6,614,909 Size: 48,720 Coding Exon Count: 8 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr8:6,566,163-6,619,024)mRNA (may differ from genome)Protein (364 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedReactomeUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: PLCE_HUMAN
DESCRIPTION: RecName: Full=1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon; EC=2.3.1.51; AltName: Full=1-acylglycerol-3-phosphate O-acyltransferase 5; Short=1-AGP acyltransferase 5; Short=1-AGPAT 5; AltName: Full=Lysophosphatidic acid acyltransferase epsilon; Short=LPAAT-epsilon;
FUNCTION: Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone (By similarity).
CATALYTIC ACTIVITY: Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.
PATHWAY: Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Potential).
DOMAIN: The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate (By similarity).
SIMILARITY: Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.
CAUTION: It is uncertain whether Met-1 or Met-12 is the initiator.
SEQUENCE CAUTION: Sequence=BAA92069.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 38.65 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 313.15 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -8.5027-0.315 Picture PostScript Text
3' UTR -994.274115-0.242 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR002123 - Acyltransferase

Pfam Domains:
PF01553 - Acyltransferase
PF16076 - Acyltransferase C-terminus

SCOP Domains:
69593 - Glycerol-3-phosphate (1)-acyltransferase

ModBase Predicted Comparative 3D Structure on Q9NUQ2
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologGenome BrowserNo ortholog
Gene DetailsGene Details  Gene Details 
Gene SorterGene Sorter  Gene Sorter 
 RGDEnsembl WormBase 
    Protein Sequence 
    Alignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003841 1-acylglycerol-3-phosphate O-acyltransferase activity
GO:0005515 protein binding
GO:0016740 transferase activity
GO:0016746 transferase activity, transferring acyl groups

Biological Process:
GO:0002244 hematopoietic progenitor cell differentiation
GO:0006629 lipid metabolic process
GO:0006639 acylglycerol metabolic process
GO:0006654 phosphatidic acid biosynthetic process
GO:0008152 metabolic process
GO:0008654 phospholipid biosynthetic process
GO:0016024 CDP-diacylglycerol biosynthetic process

Cellular Component:
GO:0005634 nucleus
GO:0005635 nuclear envelope
GO:0005739 mitochondrion
GO:0005741 mitochondrial outer membrane
GO:0005783 endoplasmic reticulum
GO:0005789 endoplasmic reticulum membrane
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  HM005457 - Homo sapiens clone HTL-T-144 testicular tissue protein Li 144 mRNA, complete cds.
LF384341 - JP 2014500723-A/191844: Polycomb-Associated Non-Coding RNAs.
BC080537 - Homo sapiens 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon), mRNA (cDNA clone MGC:99624 IMAGE:5015128), complete cds.
AF375789 - Homo sapiens lysophosphatidic acid acyltransferase-epsilon (LPAAT-e) mRNA, complete cds.
BC023550 - Homo sapiens 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon), mRNA (cDNA clone MGC:19506 IMAGE:4129973), complete cds.
AL136587 - Homo sapiens mRNA; cDNA DKFZp761C222 (from clone DKFZp761C222).
AB528060 - Synthetic construct DNA, clone: pF1KE0436, Homo sapiens AGPAT5 gene for 1-acylglycerol-3-phosphate O-acyltransferase 5, without stop codon, in Flexi system.
AM392899 - Synthetic construct Homo sapiens clone IMAGE:100001736 for hypothetical protein (AGPAT5 gene).
AM393058 - Synthetic construct Homo sapiens clone IMAGE:100001740 for hypothetical protein (AGPAT5 gene).
DQ896747 - Synthetic construct Homo sapiens clone IMAGE:100011207; FLH199432.01L; RZPDo839G1081D 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) (AGPAT5) gene, encodes complete protein.
DQ893436 - Synthetic construct clone IMAGE:100006066; FLH199526.01X; RZPDo839G1082D 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) (AGPAT5) gene, encodes complete protein.
JD058107 - Sequence 39131 from Patent EP1572962.
AK310545 - Homo sapiens cDNA, FLJ17587.
AK002072 - Homo sapiens cDNA FLJ11210 fis, clone PLACE1007954.
BC068519 - Homo sapiens 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon), mRNA (cDNA clone IMAGE:30340582), partial cds.
LF371358 - JP 2014500723-A/178861: Polycomb-Associated Non-Coding RNAs.
LF371359 - JP 2014500723-A/178862: Polycomb-Associated Non-Coding RNAs.
LF371360 - JP 2014500723-A/178863: Polycomb-Associated Non-Coding RNAs.
JD454335 - Sequence 435359 from Patent EP1572962.
JD240189 - Sequence 221213 from Patent EP1572962.
LF371361 - JP 2014500723-A/178864: Polycomb-Associated Non-Coding RNAs.
JD251060 - Sequence 232084 from Patent EP1572962.
JD160274 - Sequence 141298 from Patent EP1572962.
JD162361 - Sequence 143385 from Patent EP1572962.
JD142639 - Sequence 123663 from Patent EP1572962.
JD240627 - Sequence 221651 from Patent EP1572962.
JD435928 - Sequence 416952 from Patent EP1572962.
JD535133 - Sequence 516157 from Patent EP1572962.
JD162761 - Sequence 143785 from Patent EP1572962.
JD513318 - Sequence 494342 from Patent EP1572962.
JD087988 - Sequence 69012 from Patent EP1572962.
JD507420 - Sequence 488444 from Patent EP1572962.
JD515961 - Sequence 496985 from Patent EP1572962.
JD158452 - Sequence 139476 from Patent EP1572962.
LF371362 - JP 2014500723-A/178865: Polycomb-Associated Non-Coding RNAs.
JD499017 - Sequence 480041 from Patent EP1572962.
JD551571 - Sequence 532595 from Patent EP1572962.
JD248777 - Sequence 229801 from Patent EP1572962.
AK021722 - Homo sapiens cDNA FLJ11660 fis, clone HEMBA1004610.
MA619918 - JP 2018138019-A/191844: Polycomb-Associated Non-Coding RNAs.
MA606935 - JP 2018138019-A/178861: Polycomb-Associated Non-Coding RNAs.
MA606936 - JP 2018138019-A/178862: Polycomb-Associated Non-Coding RNAs.
MA606937 - JP 2018138019-A/178863: Polycomb-Associated Non-Coding RNAs.
MA606938 - JP 2018138019-A/178864: Polycomb-Associated Non-Coding RNAs.
MA606939 - JP 2018138019-A/178865: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  BioCyc Knowledge Library
PWY-5667 - CDP-diacylglycerol biosynthesis
TRIGLSYN-PWY - diacylglycerol and triacylglycerol biosynthesis

Reactome (by CSHL, EBI, and GO)

Protein Q9NUQ2 (Reactome details) participates in the following event(s):

R-HSA-1482548 1-acyl LPA is acylated to PA by AGPAT5 (OM)
R-HSA-75885 1-acyl LPA is acylated to PA by AGPAT (LPAAT)
R-HSA-1483166 Synthesis of PA
R-HSA-1483206 Glycerophospholipid biosynthesis
R-HSA-1483257 Phospholipid metabolism
R-HSA-556833 Metabolism of lipids
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000285518.1, ENST00000285518.10, ENST00000285518.2, ENST00000285518.3, ENST00000285518.4, ENST00000285518.5, ENST00000285518.6, ENST00000285518.7, ENST00000285518.8, ENST00000285518.9, NM_018361, PLCE_HUMAN, Q8IZ47, Q9BQG4, Q9NUQ2, uc317khv.1, uc317khv.2
UCSC ID: ENST00000285518.11_5
RefSeq Accession: NM_018361.5
Protein: Q9NUQ2 (aka PLCE_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.