Human Gene AKAP8 (ENST00000269701.7_9) from GENCODE V47lift37
  Description: A-kinase anchoring protein 8 (from RefSeq NM_005858.4)
Gencode Transcript: ENST00000269701.7_9
Gencode Gene: ENSG00000105127.10_11
Transcript (Including UTRs)
   Position: hg19 chr19:15,464,196-15,490,598 Size: 26,403 Total Exon Count: 14 Strand: -
Coding Region
   Position: hg19 chr19:15,465,726-15,490,542 Size: 24,817 Coding Exon Count: 14 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr19:15,464,196-15,490,598)mRNA (may differ from genome)Protein (692 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
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HGNCMGIOMIMPubMedUniProtKBWikipedia
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: AKAP8_HUMAN
DESCRIPTION: RecName: Full=A-kinase anchor protein 8; Short=AKAP-8; AltName: Full=A-kinase anchor protein 95 kDa; Short=AKAP 95;
FUNCTION: Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II).
SUBUNIT: Binds to dimeric RII-alpha regulatory subunit of PKA during mitosis. Interacts (via C-terminus) with FIGN (By similarity).
SUBCELLULAR LOCATION: Nucleus matrix. Note=Associated with the nuclear matrix. Redistributed and detached from condensed chromatin during mitosis.
TISSUE SPECIFICITY: Highly expressed in heart, liver, skeletal muscle, kidney and pancreas.
SIMILARITY: Belongs to the AKAP95 family.
SIMILARITY: Contains 2 C2H2 AKAP95-type zinc fingers.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 20.06 RPKM in Spleen
Total median expression: 599.34 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -17.6056-0.314 Picture PostScript Text
3' UTR -426.071530-0.278 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR007071 - AKAP95
IPR015880 - Znf_C2H2-like

Pfam Domains:
PF04988 - A-kinase anchoring protein 95 (AKAP95)

SCOP Domains:
56935 - Porins
57667 - beta-beta-alpha zinc fingers

ModBase Predicted Comparative 3D Structure on O43823
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003677 DNA binding
GO:0003690 double-stranded DNA binding
GO:0003723 RNA binding
GO:0005515 protein binding
GO:0008270 zinc ion binding
GO:0034237 protein kinase A regulatory subunit binding
GO:0042826 histone deacetylase binding
GO:0046872 metal ion binding
GO:0051059 NF-kappaB binding
GO:0017151 DEAD/H-box RNA helicase binding

Biological Process:
GO:0000278 mitotic cell cycle
GO:0002376 immune system process
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0007076 mitotic chromosome condensation
GO:0007165 signal transduction
GO:0015031 protein transport
GO:0031065 positive regulation of histone deacetylation
GO:0032720 negative regulation of tumor necrosis factor production
GO:0033127 regulation of histone phosphorylation
GO:0044839 cell cycle G2/M phase transition
GO:0045087 innate immune response
GO:0071222 cellular response to lipopolysaccharide
GO:0071380 cellular response to prostaglandin E stimulus

Cellular Component:
GO:0000793 condensed chromosome
GO:0001939 female pronucleus
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005794 Golgi apparatus
GO:0016020 membrane
GO:0016363 nuclear matrix


-  Descriptions from all associated GenBank mRNAs
  AL050160 - Homo sapiens mRNA; cDNA DKFZp586B1222 (from clone DKFZp586B1222); partial cds.
Y11997 - H.sapiens mRNA for A-kinase anchoring protein AKAP95.
BC037270 - Homo sapiens A kinase (PRKA) anchor protein 8, mRNA (cDNA clone MGC:26460 IMAGE:4837309), complete cds.
AK309529 - Homo sapiens cDNA, FLJ99570.
JD423537 - Sequence 404561 from Patent EP1572962.
JD400021 - Sequence 381045 from Patent EP1572962.
JD127811 - Sequence 108835 from Patent EP1572962.
JD234667 - Sequence 215691 from Patent EP1572962.
JD041805 - Sequence 22829 from Patent EP1572962.
JD460484 - Sequence 441508 from Patent EP1572962.
JD300549 - Sequence 281573 from Patent EP1572962.
JD316695 - Sequence 297719 from Patent EP1572962.
JD344516 - Sequence 325540 from Patent EP1572962.
JD539584 - Sequence 520608 from Patent EP1572962.
JD400417 - Sequence 381441 from Patent EP1572962.
JD053551 - Sequence 34575 from Patent EP1572962.
JD286569 - Sequence 267593 from Patent EP1572962.
JD237074 - Sequence 218098 from Patent EP1572962.
JD284677 - Sequence 265701 from Patent EP1572962.
JD428969 - Sequence 409993 from Patent EP1572962.
JD495782 - Sequence 476806 from Patent EP1572962.
JD179846 - Sequence 160870 from Patent EP1572962.
JD267891 - Sequence 248915 from Patent EP1572962.
JD100871 - Sequence 81895 from Patent EP1572962.
JD267890 - Sequence 248914 from Patent EP1572962.
JD409590 - Sequence 390614 from Patent EP1572962.
JD171470 - Sequence 152494 from Patent EP1572962.
JD367915 - Sequence 348939 from Patent EP1572962.
JD172807 - Sequence 153831 from Patent EP1572962.
JD363638 - Sequence 344662 from Patent EP1572962.
JD452131 - Sequence 433155 from Patent EP1572962.
JD311029 - Sequence 292053 from Patent EP1572962.
JD359070 - Sequence 340094 from Patent EP1572962.
JD356887 - Sequence 337911 from Patent EP1572962.
JD052980 - Sequence 34004 from Patent EP1572962.
JD201217 - Sequence 182241 from Patent EP1572962.
JD213791 - Sequence 194815 from Patent EP1572962.
JD413514 - Sequence 394538 from Patent EP1572962.
JD177258 - Sequence 158282 from Patent EP1572962.
JD171383 - Sequence 152407 from Patent EP1572962.
JD297730 - Sequence 278754 from Patent EP1572962.
JD518561 - Sequence 499585 from Patent EP1572962.
AK308251 - Homo sapiens cDNA, FLJ98199.
AK303640 - Homo sapiens cDNA FLJ50339 complete cds, highly similar to A-kinase anchor protein 8.

-  Other Names for This Gene
  Alternate Gene Symbols: AKAP8_HUMAN, AKAP95, ENST00000269701.1, ENST00000269701.2, ENST00000269701.3, ENST00000269701.4, ENST00000269701.5, ENST00000269701.6, NM_005858, O43823, uc317isf.1, uc317isf.2
UCSC ID: ENST00000269701.7_9
RefSeq Accession: NM_005858.4
Protein: O43823 (aka AKAP8_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.