Human Gene ALPK1 (ENST00000650871.1_7) from GENCODE V47lift37
  Description: alpha kinase 1, transcript variant 1 (from RefSeq NM_025144.4)
Gencode Transcript: ENST00000650871.1_7
Gencode Gene: ENSG00000073331.18_14
Transcript (Including UTRs)
   Position: hg19 chr4:113,218,525-113,363,777 Size: 145,253 Total Exon Count: 16 Strand: +
Coding Region
   Position: hg19 chr4:113,298,934-113,362,366 Size: 63,433 Coding Exon Count: 14 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesGeneReviewsModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr4:113,218,525-113,363,777)mRNA (may differ from genome)Protein (1244 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedUniProtKBWikipedia
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: ALPK1_HUMAN
DESCRIPTION: RecName: Full=Alpha-protein kinase 1; EC=2.7.11.-; AltName: Full=Chromosome 4 kinase; AltName: Full=Lymphocyte alpha-protein kinase;
FUNCTION: Kinase that recognizes phosphorylation sites in which the surrounding peptides have an alpha-helical conformation.
TISSUE SPECIFICITY: Highly expressed in liver.
SIMILARITY: Belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily.
SIMILARITY: Contains 1 alpha-type protein kinase domain.
SEQUENCE CAUTION: Sequence=BAC85140.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=BAC85140.1; Type=Frameshift; Positions=208, 1022; Sequence=BAC85140.1; Type=Miscellaneous discrepancy; Note=Intron retention; Sequence=BC060780; Type=Frameshift; Positions=1044;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
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+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -58.80253-0.232 Picture PostScript Text
3' UTR -398.201411-0.282 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011009 - Kinase-like_dom
IPR004166 - MHCK_EF2_kinase

Pfam Domains:
PF02816 - Alpha-kinase family

SCOP Domains:
56112 - Protein kinase-like (PK-like)

ModBase Predicted Comparative 3D Structure on Q96QP1
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004674 protein serine/threonine kinase activity
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity

Biological Process:
GO:0006468 protein phosphorylation
GO:0016310 phosphorylation

Cellular Component:
GO:0005737 cytoplasm


-  Descriptions from all associated GenBank mRNAs
  AK026323 - Homo sapiens cDNA: FLJ22670 fis, clone HSI08684.
AK304708 - Homo sapiens cDNA FLJ55444 complete cds, highly similar to Homo sapiens alpha-kinase 1 (ALPK1), mRNA.
AK097246 - Homo sapiens cDNA FLJ39927 fis, clone SPLEN2021194, highly similar to Homo sapiens alpha-kinase 1 (ALPK1), mRNA.
BC028117 - Homo sapiens alpha-kinase 1, mRNA (cDNA clone IMAGE:4397039), with apparent retained intron.
AK303489 - Homo sapiens cDNA FLJ61015 complete cds, highly similar to Homo sapiens alpha-kinase 1 (ALPK1), mRNA.
BX648798 - Homo sapiens mRNA; cDNA DKFZp686P21148 (from clone DKFZp686P21148).
BC060780 - Homo sapiens alpha-kinase 1, mRNA (cDNA clone IMAGE:30339169), containing frame-shift errors.
AY044164 - Homo sapiens lymphocyte alpha-kinase (LAK) mRNA, complete cds.
CU687418 - Synthetic construct Homo sapiens gateway clone IMAGE:100021041 5' read ALPK1 mRNA.
KJ903291 - Synthetic construct Homo sapiens clone ccsbBroadEn_12685 ALPK1 gene, encodes complete protein.
KJ905550 - Synthetic construct Homo sapiens clone ccsbBroadEn_15166 ALPK1 gene, encodes complete protein.
AK131090 - Homo sapiens mRNA for FLJ00302 protein.
AK091872 - Homo sapiens cDNA FLJ34553 fis, clone KIDNE2000624.
AB040960 - Homo sapiens mRNA for KIAA1527 protein, partial cds.
AK026715 - Homo sapiens cDNA: FLJ23062 fis, clone LNG04670.
AB075877 - Homo sapiens LAK mRNA for lymphocyte alpha-kinase, partial cds.
JD227438 - Sequence 208462 from Patent EP1572962.
JD143739 - Sequence 124763 from Patent EP1572962.
JD275076 - Sequence 256100 from Patent EP1572962.
JD255020 - Sequence 236044 from Patent EP1572962.
JD079880 - Sequence 60904 from Patent EP1572962.
JD267168 - Sequence 248192 from Patent EP1572962.
JD147295 - Sequence 128319 from Patent EP1572962.
JD551260 - Sequence 532284 from Patent EP1572962.
JD501410 - Sequence 482434 from Patent EP1572962.
JD224383 - Sequence 205407 from Patent EP1572962.
JD239542 - Sequence 220566 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: ALPK1 , ALPK1_HUMAN, B4E3G1, F5H138, KIAA1527 , LAK , NM_025144, Q68CI9, Q6P9F9, Q6ZNK4, Q96QP1, Q9P201, uc328sec.1, uc328sec.2
UCSC ID: ENST00000650871.1_7
RefSeq Accession: NM_025144.4
Protein: Q96QP1 (aka ALPK1_HUMAN)

-  GeneReviews for This Gene
  GeneReviews article(s) related to gene ALPK1:
alpk1-auto (ALPK1-Related Autoinflammatory Disease)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.