Human Gene AMY2B (ENST00000684275.1_2) from GENCODE V47lift37
  Description: amylase alpha 2B, transcript variant 3 (from RefSeq NM_001387437.1)
Gencode Transcript: ENST00000684275.1_2
Gencode Gene: ENSG00000240038.8_8
Transcript (Including UTRs)
   Position: hg19 chr1:104,114,211-104,122,156 Size: 7,946 Total Exon Count: 10 Strand: +
Coding Region
   Position: hg19 chr1:104,114,225-104,122,122 Size: 7,898 Coding Exon Count: 10 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathways
Other NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:104,114,211-104,122,156)mRNA (may differ from genome)Protein (511 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblExonPrimerGeneCardsHGNC
MGIPubMedReactomeUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: AMY2B_HUMAN
DESCRIPTION: RecName: Full=Alpha-amylase 2B; EC=3.2.1.1; AltName: Full=1,4-alpha-D-glucan glucanohydrolase 2B; AltName: Full=Carcinoid alpha-amylase; Flags: Precursor;
CATALYTIC ACTIVITY: Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha- linked D-glucose units.
COFACTOR: Binds 1 calcium ion per subunit (By similarity).
COFACTOR: Binds 1 chloride ion per subunit (By similarity).
SUBUNIT: Monomer (By similarity).
SUBCELLULAR LOCATION: Secreted (By similarity).
SIMILARITY: Belongs to the glycosyl hydrolase 13 family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
3' UTR 0.00340.000 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR006048 - A-amylase_b_C
IPR006046 - Alpha_amylase
IPR015902 - Glyco_hydro_13
IPR013780 - Glyco_hydro_13_b
IPR006047 - Glyco_hydro_13_cat_dom
IPR006589 - Glyco_hydro_13_sub_cat_dom
IPR013781 - Glyco_hydro_catalytic_dom
IPR017853 - Glycoside_hydrolase_SF

Pfam Domains:
PF00128 - Alpha amylase, catalytic domain
PF02806 - Alpha amylase, C-terminal all-beta domain

SCOP Domains:
51011 - Glycosyl hydrolase domain
51445 - (Trans)glycosidases

ModBase Predicted Comparative 3D Structure on P19961
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologGenome BrowserNo ortholog
 Gene Details Gene DetailsGene Details 
 Gene Sorter Gene SorterGene Sorter 
 RGD  WormBase 
    Protein Sequence 
    Alignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003824 catalytic activity
GO:0004556 alpha-amylase activity
GO:0016787 hydrolase activity
GO:0016798 hydrolase activity, acting on glycosyl bonds
GO:0043169 cation binding
GO:0046872 metal ion binding

Biological Process:
GO:0005975 carbohydrate metabolic process
GO:0008152 metabolic process

Cellular Component:
GO:0005576 extracellular region
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  AB058742 - Homo sapiens KIAA1839 mRNA for KIAA1839 protein.
AL832291 - Homo sapiens mRNA; cDNA DKFZp667O0118 (from clone DKFZp667O0118).
AK095605 - Homo sapiens cDNA FLJ38286 fis, clone FCBBF3008153, highly similar to ALPHA-AMYLASE 2B PRECURSOR (EC 3.2.1.1).
BC011179 - Homo sapiens amylase, alpha 2B (pancreatic), mRNA (cDNA clone MGC:17195 IMAGE:4150646), complete cds.
BC020861 - Homo sapiens amylase, alpha 2B (pancreatic), mRNA (cDNA clone MGC:24057 IMAGE:4551745), complete cds.
AK127047 - Homo sapiens cDNA FLJ45104 fis, clone BRAWH3032930, highly similar to Alpha-amylase 2B precursor (EC 3.2.1.1).
M24895 - Homo sapiens alpha-amylase mRNA, complete cds.
CU676750 - Synthetic construct Homo sapiens gateway clone IMAGE:100022298 5' read AMY2B mRNA.
KJ896418 - Synthetic construct Homo sapiens clone ccsbBroadEn_05812 AMY2B gene, encodes complete protein.
KJ896419 - Synthetic construct Homo sapiens clone ccsbBroadEn_05813 AMY2B gene, encodes complete protein.
DQ786288 - Homo sapiens clone HLS_IMAGE_488945 mRNA sequence.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein P19961 (Reactome details) participates in the following event(s):

R-HSA-188979 Digestion of linear starch (amylose) by extracellular amylase
R-HSA-191114 Digestion of branched starch (amylopectin) by extracellular amylase
R-HSA-189085 Digestion of dietary carbohydrate
R-HSA-8935690 Digestion
R-HSA-8963743 Digestion and absorption

-  Other Names for This Gene
  Alternate Gene Symbols: AMY2B_HUMAN, B3KTI1, B3KXB7, D3DT76, NM_001387437, P19961, Q9UBH3, uc330mqd.1, uc330mqd.2
UCSC ID: ENST00000684275.1_2
RefSeq Accession: NM_001387437.1
Protein: P19961 (aka AMY2B_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.