Human Gene ANXA4 (ENST00000394295.6_4) from GENCODE V47lift37
  Description: annexin A4, transcript variant 5 (from RefSeq NM_001365496.2)
Gencode Transcript: ENST00000394295.6_4
Gencode Gene: ENSG00000196975.16_8
Transcript (Including UTRs)
   Position: hg19 chr2:69,969,266-70,054,244 Size: 84,979 Total Exon Count: 13 Strand: +
Coding Region
   Position: hg19 chr2:70,008,698-70,052,647 Size: 43,950 Coding Exon Count: 12 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr2:69,969,266-70,054,244)mRNA (may differ from genome)Protein (321 aa)
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BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: ANXA4_HUMAN
DESCRIPTION: RecName: Full=Annexin A4; AltName: Full=35-beta calcimedin; AltName: Full=Annexin IV; AltName: Full=Annexin-4; AltName: Full=Carbohydrate-binding protein p33/p41; AltName: Full=Chromobindin-4; AltName: Full=Endonexin I; AltName: Full=Lipocortin IV; AltName: Full=P32.5; AltName: Full=PP4-X; AltName: Full=Placental anticoagulant protein II; Short=PAP-II; AltName: Full=Protein II;
FUNCTION: Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis (By similarity).
DOMAIN: A pair of annexin repeats may form one binding site for calcium and phospholipid.
MISCELLANEOUS: Seems to bind one calcium ion with high affinity.
SIMILARITY: Belongs to the annexin family.
SIMILARITY: Contains 4 annexin repeats.
SEQUENCE CAUTION: Sequence=AAC41689.1; Type=Erroneous initiation; Sequence=AAH00182.1; Type=Erroneous initiation; Sequence=AAH11659.1; Type=Erroneous initiation; Sequence=BAA11227.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 80.20 RPKM in Pancreas
Total median expression: 888.57 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -26.6088-0.302 Picture PostScript Text
3' UTR -359.101597-0.225 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001464 - Annexin
IPR018502 - Annexin_repeat
IPR018252 - Annexin_repeat_CS
IPR002391 - AnnexinIV

Pfam Domains:
PF00191 - Annexin

SCOP Domains:
47874 - Annexin

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2ZOC - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P09525
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004859 phospholipase inhibitor activity
GO:0005509 calcium ion binding
GO:0005515 protein binding
GO:0005544 calcium-dependent phospholipid binding
GO:0042802 identical protein binding
GO:0048306 calcium-dependent protein binding
GO:0051059 NF-kappaB binding

Biological Process:
GO:0006357 regulation of transcription from RNA polymerase II promoter
GO:0007165 signal transduction
GO:0007219 Notch signaling pathway
GO:0030855 epithelial cell differentiation
GO:0032088 negative regulation of NF-kappaB transcription factor activity
GO:0043066 negative regulation of apoptotic process
GO:0043086 negative regulation of catalytic activity
GO:2000483 negative regulation of interleukin-8 secretion

Cellular Component:
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005886 plasma membrane
GO:0009986 cell surface
GO:0012506 vesicle membrane
GO:0031965 nuclear membrane
GO:0048471 perinuclear region of cytoplasm
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  AB209457 - Homo sapiens mRNA for annexin IV variant protein.
BC063672 - Homo sapiens annexin A4, mRNA (cDNA clone MGC:75105 IMAGE:3908264), complete cds.
BC000182 - Homo sapiens annexin A4, mRNA (cDNA clone MGC:2271 IMAGE:3352245), complete cds.
AK293177 - Homo sapiens cDNA FLJ52218 complete cds, highly similar to Annexin A4.
BC011659 - Homo sapiens annexin A4, mRNA (cDNA clone MGC:14409 IMAGE:4301675), complete cds.
AK303960 - Homo sapiens cDNA FLJ51185 complete cds, highly similar to Annexin A4.
AK293406 - Homo sapiens cDNA FLJ52120 complete cds, highly similar to Annexin A4.
AK315511 - Homo sapiens cDNA, FLJ96576, Homo sapiens annexin A4 (ANXA4), mRNA.
AK058017 - Homo sapiens cDNA FLJ25288 fis, clone STM07104, highly similar to ANNEXIN IV.
AK293396 - Homo sapiens cDNA FLJ51794 complete cds, highly similar to Annexin A4.
FJ224287 - Homo sapiens epididymis secretory protein Li 274 (HEL-S-274) mRNA, complete cds.
M19383 - Human protein PP4-X mRNA, complete cds.
M82809 - Human annexin IV (ANX4) mRNA, complete cds.
D78152 - Homo sapiens mRNA for annexin IV (carbohydrate-binding protein p33/41), complete cds.
AY453398 - Homo sapiens proliferation-inducing protein 28 (PIG28) mRNA, complete cds.
JD321324 - Sequence 302348 from Patent EP1572962.
CU674078 - Synthetic construct Homo sapiens gateway clone IMAGE:100020663 5' read ANXA4 mRNA.
CU687798 - Synthetic construct Homo sapiens gateway clone IMAGE:100021660 5' read ANXA4 mRNA.
KJ890675 - Synthetic construct Homo sapiens clone ccsbBroadEn_00069 ANXA4 gene, encodes complete protein.
KJ901285 - Synthetic construct Homo sapiens clone ccsbBroadEn_10679 ANXA4 gene, encodes complete protein.
CR407681 - Homo sapiens full open reading frame cDNA clone RZPDo834D013D for gene ANXA4, annexin A4 complete cds, without stopcodon.
DQ896101 - Synthetic construct Homo sapiens clone IMAGE:100010561; FLH190317.01L; RZPDo839G1265D annexin A4 (ANXA4) gene, encodes complete protein.
DQ892854 - Synthetic construct clone IMAGE:100005484; FLH190321.01X; RZPDo839G1275D annexin A4 (ANXA4) gene, encodes complete protein.
BX641114 - Homo sapiens mRNA; cDNA DKFZp686H02120 (from clone DKFZp686H02120).
JD251172 - Sequence 232196 from Patent EP1572962.
JD091121 - Sequence 72145 from Patent EP1572962.
JD038725 - Sequence 19749 from Patent EP1572962.
JD554402 - Sequence 535426 from Patent EP1572962.
JD114578 - Sequence 95602 from Patent EP1572962.
JD279485 - Sequence 260509 from Patent EP1572962.
LF207256 - JP 2014500723-A/14759: Polycomb-Associated Non-Coding RNAs.
JD225733 - Sequence 206757 from Patent EP1572962.
JD292616 - Sequence 273640 from Patent EP1572962.
JD497720 - Sequence 478744 from Patent EP1572962.
JD542661 - Sequence 523685 from Patent EP1572962.
JD076579 - Sequence 57603 from Patent EP1572962.
JD027862 - Sequence 8886 from Patent EP1572962.
JD178862 - Sequence 159886 from Patent EP1572962.
MA442833 - JP 2018138019-A/14759: Polycomb-Associated Non-Coding RNAs.

-  Other Names for This Gene
  Alternate Gene Symbols: ANX4, ANXA4 , ANXA4_HUMAN, B4DDF9, ENST00000394295.1, ENST00000394295.2, ENST00000394295.3, ENST00000394295.4, ENST00000394295.5, NM_001365496, P09525, Q6LES2, Q96F33, Q9BWK1, uc318wny.1, uc318wny.2
UCSC ID: ENST00000394295.6_4
RefSeq Accession: NM_001153.5
Protein: P09525 (aka ANXA4_HUMAN or ANX4_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.