Human Gene AP1G2 (ENST00000397120.8_7) from GENCODE V47lift37
  Description: adaptor related protein complex 1 subunit gamma 2, transcript variant 11 (from RefSeq NR_148938.2)
Gencode Transcript: ENST00000397120.8_7
Gencode Gene: ENSG00000213983.12_10
Transcript (Including UTRs)
   Position: hg19 chr14:24,028,776-24,037,000 Size: 8,225 Total Exon Count: 22 Strand: -
Coding Region
   Position: hg19 chr14:24,028,958-24,036,523 Size: 7,566 Coding Exon Count: 21 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr14:24,028,776-24,037,000)mRNA (may differ from genome)Protein (785 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
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UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: AP1G2_HUMAN
DESCRIPTION: RecName: Full=AP-1 complex subunit gamma-like 2; AltName: Full=Gamma2-adaptin; Short=G2ad;
FUNCTION: May function in protein sorting in late endosomes or multivesucular bodies (MVBs). Involved in MVB-assisted maturation of hepatitis B virus (HBV).
SUBUNIT: Probably interacts with AP1S1/Sigma1A-adaptin AP1S2/Sigma1B-adaptin. Probably does not interact with APB1. Interacts with HBV major surface antigen L. Interacts with HBV core protein C in a ubiquitin-dependent manner. Binds ubiquitin.
INTERACTION: Q15276:RABEP1; NbExp=2; IntAct=EBI-373637, EBI-447043;
SUBCELLULAR LOCATION: Golgi apparatus membrane; Peripheral membrane protein. Cytoplasmic vesicle membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Note=Mainly localized to perinuclear vesicular structures. Colocalizes with HBV major surface antigen L and HBV core protein C in CD63-containing compartments. Colocalizes with HBV major surface antigen L to cis-Golgi-like structures.
TISSUE SPECIFICITY: Expressed in all but one (skeletal muscle) tissues examined.
SIMILARITY: Belongs to the adaptor complexes large subunit family.
SIMILARITY: Contains 1 GAE domain.
CAUTION: Does not appear to be a subunit of the clathrin- associated adaptor protein complex 1 (AP-1).
SEQUENCE CAUTION: Sequence=AAC67390.1; Type=Frameshift; Positions=713;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: AP1G2
Diseases sorted by gene-association score: cardiac arrest (3), long qt syndrome 1 (1)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 131.48 RPKM in Brain - Cerebellum
Total median expression: 1674.33 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -14.8058-0.255 Picture PostScript Text
3' UTR -50.50182-0.277 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR017107 - AP1_complex_gsu
IPR011989 - ARM-like
IPR016024 - ARM-type_fold
IPR002553 - Clathrin/coatomer_adapt-like_N
IPR008152 - Clathrin_a/b/g-adaptin_app_Ig
IPR008153 - Clathrin_g-adaptin_app
IPR013041 - Coatomer/clathrin_app_Ig-like

Pfam Domains:
PF01602 - Adaptin N terminal region
PF02883 - Adaptin C-terminal domain
PF12717 - non-SMC mitotic condensation complex subunit 1

SCOP Domains:
48371 - ARM repeat
48431 - Lipovitellin-phosvitin complex, superhelical domain
49348 - Clathrin adaptor appendage domain

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2E9G - NMR MuPIT


ModBase Predicted Comparative 3D Structure on O75843
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding

Biological Process:
GO:0006886 intracellular protein transport
GO:0015031 protein transport
GO:0016032 viral process
GO:0016192 vesicle-mediated transport

Cellular Component:
GO:0000139 Golgi membrane
GO:0005768 endosome
GO:0005794 Golgi apparatus
GO:0005798 Golgi-associated vesicle
GO:0010008 endosome membrane
GO:0016020 membrane
GO:0030117 membrane coat
GO:0030121 AP-1 adaptor complex
GO:0030131 clathrin adaptor complex
GO:0030133 transport vesicle
GO:0030659 cytoplasmic vesicle membrane
GO:0031410 cytoplasmic vesicle


-  Descriptions from all associated GenBank mRNAs
  BC131549 - Homo sapiens cDNA clone IMAGE:40084966.
AK304682 - Homo sapiens cDNA FLJ56213 complete cds, highly similar to Adapter-relatedprotein complex 1 gamma- 2 subunit.
AK096034 - Homo sapiens cDNA FLJ38715 fis, clone KIDNE2009605, highly similar to Homo sapiens mRNA for gamma2-adaptin.
AK123354 - Homo sapiens cDNA FLJ41360 fis, clone BRCAN2002948.
AB015318 - Homo sapiens mRNA for gamma2-adaptin, complete cds.
AF068706 - Homo sapiens gamma2-adaptin (G2AD) mRNA, complete cds.
FW340082 - Screening.
BC051833 - Homo sapiens adaptor-related protein complex 1, gamma 2 subunit, mRNA (cDNA clone MGC:60125 IMAGE:5589077), complete cds.
AK226158 - Homo sapiens mRNA for adaptor-related protein complex 1, gamma 2 subunit variant, clone: sh00690.
JD391326 - Sequence 372350 from Patent EP1572962.
JD060724 - Sequence 41748 from Patent EP1572962.
BX161425 - human full-length cDNA clone CS0DI003YF22 of Placenta of Homo sapiens (human).
JD372190 - Sequence 353214 from Patent EP1572962.
JD290188 - Sequence 271212 from Patent EP1572962.
JD228359 - Sequence 209383 from Patent EP1572962.
KJ901924 - Synthetic construct Homo sapiens clone ccsbBroadEn_11318 AP1G2 gene, encodes complete protein.
BX247963 - human full-length cDNA clone CS0DE001YK01 of Placenta of Homo sapiens (human).
AK309103 - Homo sapiens cDNA, FLJ99144.
AK307696 - Homo sapiens cDNA, FLJ97644.
AK310451 - Homo sapiens cDNA, FLJ17493.
JD025530 - Sequence 6554 from Patent EP1572962.
JD033894 - Sequence 14918 from Patent EP1572962.
JD206440 - Sequence 187464 from Patent EP1572962.
JD484414 - Sequence 465438 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein O75843 (Reactome details) participates in the following event(s):

R-HSA-5671702 AP1G2 binds HGS and CHMP2A
R-HSA-432720 Lysosome Vesicle Biogenesis
R-HSA-421837 Clathrin derived vesicle budding
R-HSA-199992 trans-Golgi Network Vesicle Budding
R-HSA-199991 Membrane Trafficking
R-HSA-5653656 Vesicle-mediated transport

-  Other Names for This Gene
  Alternate Gene Symbols: AP1G2_HUMAN, D3DS51, ENST00000397120.1, ENST00000397120.2, ENST00000397120.3, ENST00000397120.4, ENST00000397120.5, ENST00000397120.6, ENST00000397120.7, NR_148938, O75504, O75843, uc318ytp.1, uc318ytp.2
UCSC ID: ENST00000397120.8_7
RefSeq Accession: NM_003917.5
Protein: O75843 (aka AP1G2_HUMAN or A1G2_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.