Human Gene API5 (ENST00000531273.6_6) from GENCODE V47lift37
  Description: apoptosis inhibitor 5, transcript variant 1 (from RefSeq NM_001142930.2)
Gencode Transcript: ENST00000531273.6_6
Gencode Gene: ENSG00000166181.13_11
Transcript (Including UTRs)
   Position: hg19 chr11:43,333,546-43,366,079 Size: 32,534 Total Exon Count: 14 Strand: +
Coding Region
   Position: hg19 chr11:43,333,678-43,364,060 Size: 30,383 Coding Exon Count: 14 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr11:43,333,546-43,366,079)mRNA (may differ from genome)Protein (524 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedUniProtKBWikipedia
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: API5_HUMAN
DESCRIPTION: RecName: Full=Apoptosis inhibitor 5; Short=API-5; AltName: Full=Antiapoptosis clone 11 protein; Short=AAC-11; AltName: Full=Cell migration-inducing gene 8 protein; AltName: Full=Fibroblast growth factor 2-interacting factor; Short=FIF; AltName: Full=Protein XAGL;
FUNCTION: Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.
SUBUNIT: Monomer. Interacts with FGF2 and ACIN1.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Mainly nuclear. Can also be cytoplasmic.
SUBCELLULAR LOCATION: Isoform 3: Cytoplasm.
TISSUE SPECIFICITY: Expressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in heart, pancreas and placenta. Highly expressed in several cancers. Preferentially expressed in squamous cell carcinoma versus adenocarcinoma in non-small cell lung cancer.
DOMAIN: Two regions, an N-terminal (aa 96-107) and a C-terminal (aa 274-311) are required for binding FGF2.
PTM: Acetylation at Lys-251 impairs antiapoptotic function.
SIMILARITY: Belongs to the API5 family.
SEQUENCE CAUTION: Sequence=AAB86528.1; Type=Erroneous initiation; Note=Translation N-terminally shortened; Sequence=AAB86528.1; Type=Frameshift; Positions=Several;
WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/api5/";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 39.16 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 979.72 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -53.00132-0.402 Picture PostScript Text
3' UTR -471.502019-0.234 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR008383 - API5
IPR011989 - ARM-like
IPR016024 - ARM-type_fold

Pfam Domains:
PF05918 - Apoptosis inhibitory protein 5 (API5)

SCOP Domains:
48371 - ARM repeat
48431 - Lipovitellin-phosvitin complex, superhelical domain

Protein Data Bank (PDB) 3-D Structure
MuPIT help
3U0R - X-ray MuPIT 3V6A - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q9BZZ5
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003723 RNA binding
GO:0005515 protein binding
GO:0017134 fibroblast growth factor binding

Biological Process:
GO:0006915 apoptotic process
GO:0043066 negative regulation of apoptotic process
GO:2000270 negative regulation of fibroblast apoptotic process

Cellular Component:
GO:0005634 nucleus
GO:0005681 spliceosomal complex
GO:0005737 cytoplasm
GO:0016020 membrane
GO:0016607 nuclear speck


-  Descriptions from all associated GenBank mRNAs
  LF205791 - JP 2014500723-A/13294: Polycomb-Associated Non-Coding RNAs.
AK299288 - Homo sapiens cDNA FLJ61353 complete cds, highly similar to Apoptosis inhibitor 5.
AK304157 - Homo sapiens cDNA FLJ51754 complete cds, highly similar to Apoptosis inhibitor 5.
AK294724 - Homo sapiens cDNA FLJ53886 complete cds, highly similar to Apoptosis inhibitor 5.
Y15906 - Homo sapiens mRNA for XAGL protein.
AF229254 - Homo sapiens clone FIF 504 fibroblast growth factor 2-interacting factor (API5) mRNA, complete cds.
AY311389 - Homo sapiens migration-inducing protein MIG8 mRNA, complete cds.
U83857 - Human Aac11 (aac11) mRNA, complete cds.
BC017709 - Homo sapiens apoptosis inhibitor 5, mRNA (cDNA clone MGC:21216 IMAGE:4419401), complete cds.
AK293303 - Homo sapiens cDNA FLJ52148 complete cds, highly similar to Apoptosis inhibitor 5.
DQ893852 - Synthetic construct Homo sapiens clone IMAGE:100008312; FLH164959.01L; RZPDo839E01157D apoptosis inhibitor 5 (API5) gene, encodes complete protein.
DQ890671 - Synthetic construct clone IMAGE:100003301; FLH164963.01X; RZPDo839E01158D apoptosis inhibitor 5 (API5) gene, encodes complete protein.
BT007093 - Homo sapiens apoptosis inhibitor 5 mRNA, complete cds.
LF348289 - JP 2014500723-A/155792: Polycomb-Associated Non-Coding RNAs.
AF229253 - Homo sapiens clone FIF N1 fibroblast growth factor 2-interacting factor (API5) mRNA, partial cds.; alternatively spliced.
LF348288 - JP 2014500723-A/155791: Polycomb-Associated Non-Coding RNAs.
LF348287 - JP 2014500723-A/155790: Polycomb-Associated Non-Coding RNAs.
LF348286 - JP 2014500723-A/155789: Polycomb-Associated Non-Coding RNAs.
JD540288 - Sequence 521312 from Patent EP1572962.
LF348285 - JP 2014500723-A/155788: Polycomb-Associated Non-Coding RNAs.
JD360846 - Sequence 341870 from Patent EP1572962.
JD349894 - Sequence 330918 from Patent EP1572962.
JD353355 - Sequence 334379 from Patent EP1572962.
JD528739 - Sequence 509763 from Patent EP1572962.
JD146865 - Sequence 127889 from Patent EP1572962.
JD400337 - Sequence 381361 from Patent EP1572962.
LF348284 - JP 2014500723-A/155787: Polycomb-Associated Non-Coding RNAs.
BC015898 - Homo sapiens, clone IMAGE:3907464, mRNA.
LF348281 - JP 2014500723-A/155784: Polycomb-Associated Non-Coding RNAs.
JD351972 - Sequence 332996 from Patent EP1572962.
LF348280 - JP 2014500723-A/155783: Polycomb-Associated Non-Coding RNAs.
JD291929 - Sequence 272953 from Patent EP1572962.
JD046088 - Sequence 27112 from Patent EP1572962.
JD284877 - Sequence 265901 from Patent EP1572962.
JD113472 - Sequence 94496 from Patent EP1572962.
JD113473 - Sequence 94497 from Patent EP1572962.
JD046248 - Sequence 27272 from Patent EP1572962.
JD281100 - Sequence 262124 from Patent EP1572962.
JD280889 - Sequence 261913 from Patent EP1572962.
JD280888 - Sequence 261912 from Patent EP1572962.
JD301313 - Sequence 282337 from Patent EP1572962.
JD117995 - Sequence 99019 from Patent EP1572962.
JD410844 - Sequence 391868 from Patent EP1572962.
JD082566 - Sequence 63590 from Patent EP1572962.
JD207219 - Sequence 188243 from Patent EP1572962.
LF348279 - JP 2014500723-A/155782: Polycomb-Associated Non-Coding RNAs.
JD333211 - Sequence 314235 from Patent EP1572962.
LF205790 - JP 2014500723-A/13293: Polycomb-Associated Non-Coding RNAs.
JD555007 - Sequence 536031 from Patent EP1572962.
JD297837 - Sequence 278861 from Patent EP1572962.
LF348278 - JP 2014500723-A/155781: Polycomb-Associated Non-Coding RNAs.
JD132418 - Sequence 113442 from Patent EP1572962.
JD281093 - Sequence 262117 from Patent EP1572962.
JD045072 - Sequence 26096 from Patent EP1572962.
JD311458 - Sequence 292482 from Patent EP1572962.
LF348277 - JP 2014500723-A/155780: Polycomb-Associated Non-Coding RNAs.
JD137395 - Sequence 118419 from Patent EP1572962.
MA583866 - JP 2018138019-A/155792: Polycomb-Associated Non-Coding RNAs.
MA583865 - JP 2018138019-A/155791: Polycomb-Associated Non-Coding RNAs.
MA583864 - JP 2018138019-A/155790: Polycomb-Associated Non-Coding RNAs.
MA583863 - JP 2018138019-A/155789: Polycomb-Associated Non-Coding RNAs.
MA583862 - JP 2018138019-A/155788: Polycomb-Associated Non-Coding RNAs.
MA583861 - JP 2018138019-A/155787: Polycomb-Associated Non-Coding RNAs.
MA583858 - JP 2018138019-A/155784: Polycomb-Associated Non-Coding RNAs.
MA583857 - JP 2018138019-A/155783: Polycomb-Associated Non-Coding RNAs.
MA583856 - JP 2018138019-A/155782: Polycomb-Associated Non-Coding RNAs.
MA583855 - JP 2018138019-A/155781: Polycomb-Associated Non-Coding RNAs.
MA583854 - JP 2018138019-A/155780: Polycomb-Associated Non-Coding RNAs.
MA441368 - JP 2018138019-A/13294: Polycomb-Associated Non-Coding RNAs.
MA441367 - JP 2018138019-A/13293: Polycomb-Associated Non-Coding RNAs.

-  Other Names for This Gene
  Alternate Gene Symbols: API5 , API5_HUMAN, B4DGR0, B4DRJ2, B4E283, D3DR21, ENST00000531273.1, ENST00000531273.2, ENST00000531273.3, ENST00000531273.4, ENST00000531273.5, G3V1C3, MIG8, NM_001142930, O15441, Q9BZZ5, Q9Y4J7, uc324iof.1, uc324iof.2
UCSC ID: ENST00000531273.6_6
RefSeq Accession: NM_001142930.2
Protein: Q9BZZ5 (aka API5_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.