ID:ARF1_HUMAN DESCRIPTION: RecName: Full=ADP-ribosylation factor 1; FUNCTION: GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. SUBUNIT: Interacts with PRKCABP, GGA1 and PIP5K1B (By similarity). Interacts with ARFGAP1, which hydrolyzes GTP and thus, regulates its function. Interacts with ASAP2, HERC1 and ARHGAP21. Interacts with PI4KB in the Golgi complex. Interacts with NCS1/FREQ in the Golgi and at the plasma membrane. This interaction at the plasma membrane requires a myristoylated NCS1/FREQ. Interacts with TMED2 and TMED10. Interacts with PLEKHA3. Interacts with PLEKHA8; the interaction, together with phosphatidylinositol 4-phosphate binding, is required for the FAPP2-mediated glucosylceramide transfer activity. INTERACTION: Q99418:CYTH2; NbExp=4; IntAct=EBI-447171, EBI-448974; SUBCELLULAR LOCATION: Golgi apparatus. Cytoplasm, perinuclear region. SIMILARITY: Belongs to the small GTPase superfamily. Arf family.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
Pfam Domains: PF00025 - ADP-ribosylation factor family PF00071 - Ras family PF01926 - 50S ribosome-binding GTPase PF04670 - Gtr1/RagA G protein conserved region PF08477 - Ras of Complex, Roc, domain of DAPkinase PF09439 - Signal recognition particle receptor beta subunit
ModBase Predicted Comparative 3D Structure on P84077
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0002090 regulation of receptor internalization GO:0006661 phosphatidylinositol biosynthetic process GO:0006878 cellular copper ion homeostasis GO:0015031 protein transport GO:0016192 vesicle-mediated transport GO:0019886 antigen processing and presentation of exogenous peptide antigen via MHC class II GO:0034315 regulation of Arp2/3 complex-mediated actin nucleation GO:0035722 interleukin-12-mediated signaling pathway GO:0050690 regulation of defense response to virus by virus GO:0060292 long term synaptic depression GO:0097061 dendritic spine organization GO:0098586 cellular response to virus
AK000620 - Homo sapiens cDNA FLJ20613 fis, clone KAT05486, highly similar to M36340 Human ADP-ribosylation factor 1. LF385528 - JP 2014500723-A/193031: Polycomb-Associated Non-Coding RNAs. BC011358 - Homo sapiens ADP-ribosylation factor 1, mRNA (cDNA clone MGC:9347 IMAGE:3463523), complete cds. AK293553 - Homo sapiens cDNA FLJ61099 complete cds, highly similar to ADP-ribosylation factor 1. AK297792 - Homo sapiens cDNA FLJ51902 complete cds, highly similar to ADP-ribosylation factor 1. AK057875 - Homo sapiens cDNA FLJ25146 fis, clone CBR07332, highly similar to ADP-RIBOSYLATION FACTOR 1. AF052179 - Homo sapiens clone 24537 ADP-ribosylation factor 1 mRNA, complete cds. M36340 - Human ADP-ribosylation factor 1 (ARF1) mRNA, complete cds. AK130737 - Homo sapiens cDNA FLJ27227 fis, clone SYN05501, highly similar to ADP-ribosylation factor 1. AF055002 - Homo sapiens clone 24614 ADP-ribosylation factor 1 mRNA, complete cds. M84326 - Human ADP-ribosylation factor 1 mRNA, complete cds. AK057838 - Homo sapiens cDNA FLJ25109 fis, clone CBR01561, highly similar to ADP-RIBOSYLATION FACTOR 1. BC010429 - Homo sapiens ADP-ribosylation factor 1, mRNA (cDNA clone MGC:15747 IMAGE:3356859), complete cds. JD159804 - Sequence 140828 from Patent EP1572962. AK296020 - Homo sapiens cDNA FLJ51215 complete cds, highly similar to ADP-ribosylation factor 1. BC009247 - Homo sapiens ADP-ribosylation factor 1, mRNA (cDNA clone MGC:3958 IMAGE:2963635), complete cds. KJ890700 - Synthetic construct Homo sapiens clone ccsbBroadEn_00094 ARF1 gene, encodes complete protein. AB385094 - Synthetic construct DNA, clone: pF1KB5398, Homo sapiens ARF1 gene for ADP-ribosylation factor 1, complete cds, without stop codon, in Flexi system. AM393462 - Synthetic construct Homo sapiens clone IMAGE:100002742 for hypothetical protein (ARF1 gene). AM393595 - Synthetic construct Homo sapiens clone IMAGE:100002743 for hypothetical protein (ARF1 gene). AM393261 - Synthetic construct Homo sapiens clone IMAGE:100002992 for hypothetical protein (ARF1 gene). AM393712 - Synthetic construct Homo sapiens clone IMAGE:100002993 for hypothetical protein (ARF1 gene). BT007393 - Homo sapiens ADP-ribosylation factor 1 mRNA, complete cds. AF493881 - Homo sapiens ADP-ribosylation factor protein 1 (ARF1) mRNA, complete cds. LF209085 - JP 2014500723-A/16588: Polycomb-Associated Non-Coding RNAs. LF325964 - JP 2014500723-A/133467: Polycomb-Associated Non-Coding RNAs. DQ579447 - Homo sapiens piRNA piR-47559, complete sequence. LF325962 - JP 2014500723-A/133465: Polycomb-Associated Non-Coding RNAs. LF325960 - JP 2014500723-A/133463: Polycomb-Associated Non-Coding RNAs. BC010415 - Homo sapiens ADP-ribosylation factor 1, mRNA (cDNA clone IMAGE:4024984), partial cds. JD254480 - Sequence 235504 from Patent EP1572962. JD454310 - Sequence 435334 from Patent EP1572962. JD545467 - Sequence 526491 from Patent EP1572962. LF325958 - JP 2014500723-A/133461: Polycomb-Associated Non-Coding RNAs. JD241847 - Sequence 222871 from Patent EP1572962. JD099874 - Sequence 80898 from Patent EP1572962. JD322829 - Sequence 303853 from Patent EP1572962. LF325957 - JP 2014500723-A/133460: Polycomb-Associated Non-Coding RNAs. JD114236 - Sequence 95260 from Patent EP1572962. JD097232 - Sequence 78256 from Patent EP1572962. JD052622 - Sequence 33646 from Patent EP1572962. JD389770 - Sequence 370794 from Patent EP1572962. JD458187 - Sequence 439211 from Patent EP1572962. JD252153 - Sequence 233177 from Patent EP1572962. JD484615 - Sequence 465639 from Patent EP1572962. JD458375 - Sequence 439399 from Patent EP1572962. JD335910 - Sequence 316934 from Patent EP1572962. JD399353 - Sequence 380377 from Patent EP1572962. JD283867 - Sequence 264891 from Patent EP1572962. LF325956 - JP 2014500723-A/133459: Polycomb-Associated Non-Coding RNAs. JD093567 - Sequence 74591 from Patent EP1572962. LF325955 - JP 2014500723-A/133458: Polycomb-Associated Non-Coding RNAs. JD428754 - Sequence 409778 from Patent EP1572962. MA621105 - JP 2018138019-A/193031: Polycomb-Associated Non-Coding RNAs. MA561541 - JP 2018138019-A/133467: Polycomb-Associated Non-Coding RNAs. MA561539 - JP 2018138019-A/133465: Polycomb-Associated Non-Coding RNAs. MA561537 - JP 2018138019-A/133463: Polycomb-Associated Non-Coding RNAs. MA561535 - JP 2018138019-A/133461: Polycomb-Associated Non-Coding RNAs. MA561534 - JP 2018138019-A/133460: Polycomb-Associated Non-Coding RNAs. MA561533 - JP 2018138019-A/133459: Polycomb-Associated Non-Coding RNAs. MA561532 - JP 2018138019-A/133458: Polycomb-Associated Non-Coding RNAs. MA444662 - JP 2018138019-A/16588: Polycomb-Associated Non-Coding RNAs.
Biochemical and Signaling Pathways
BioCarta from NCI Cancer Genome Anatomy Project h_ptdinsPathway - Phosphoinositides and their downstream targets. h_arapPathway - ADP-Ribosylation Factor
Reactome (by CSHL, EBI, and GO)
Protein P84077 (Reactome details) participates in the following event(s):
R-HSA-8870499 PLEKHA3,8 bind PI4P, ARF1 R-HSA-200879 Formation of a Nef:ARF1:CD4 complex R-HSA-421833 Vamp And trans-Golgi Network AP-1 Binding Coupled With Cargo Capture R-HSA-432712 Vamp And trans-Golgi Network AP-1 Binding Coupled With Cargo Capture On Lysosome Vesicle Destined Golgi Membrane R-HSA-350769 trans-Golgi Network Coat Activation R-HSA-8847875 ARF1:GTP binds Golgin TRIP11 R-HSA-8847880 CYTH proteins bind ARF1:GTP R-HSA-421831 trans-Golgi Network Coat Assembly R-HSA-432706 trans-Golgi Network Lysosome Vesicle Destined Membrane Coat Assembly R-HSA-8951498 Dissociation of Arf1:GDP, AP-1 Clathrin coated nonameric complex R-HSA-8847883 CYTH proteins stimulate ARF1 GTPase activity R-HSA-1676152 PI4KB binds to ARF1/3:GTP at the Golgi membrane R-HSA-6807866 GBF1 recruits ARF:GDP to the ERGIC R-HSA-6811411 GBF1 recruits ARF:GDP to the Golgi R-HSA-6807877 ARFGAPs stimulate ARF GTPase activity R-HSA-6811418 ARFGAPs stimulate ARF GTPase activity at the Golgi membrane R-HSA-6807868 GBF1 stimulates ARF nucleotide exchange R-HSA-6811414 GBF1 stimulates nucleotide exchange on ARF R-HSA-6807872 Active ARF recruits coatomer R-HSA-6811412 Active ARF recruits coatomer to the Golgi R-HSA-1675883 PI is phosphorylated to PI4P by PI4KB at the Golgi membrane R-HSA-6807875 ARFGAP, cargo, v-SNAREs and p24 proteins bind nascent COPI complex R-HSA-6811417 ARFGAP, cargo, vSNARES and p24 proteins bind COPI vesicles at Golgi R-HSA-1660499 Synthesis of PIPs at the plasma membrane R-HSA-167590 Nef Mediated CD4 Down-regulation R-HSA-8950505 Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation R-HSA-432722 Golgi Associated Vesicle Biogenesis R-HSA-432720 Lysosome Vesicle Biogenesis R-HSA-2132295 MHC class II antigen presentation R-HSA-421837 Clathrin derived vesicle budding R-HSA-6811438 Intra-Golgi traffic R-HSA-1483255 PI Metabolism R-HSA-164938 Nef-mediates down modulation of cell surface receptors by recruiting them to clathrin adapters R-HSA-9020591 Interleukin-12 signaling R-HSA-1280218 Adaptive Immune System R-HSA-199992 trans-Golgi Network Vesicle Budding R-HSA-1660514 Synthesis of PIPs at the Golgi membrane R-HSA-6811442 Intra-Golgi and retrograde Golgi-to-ER traffic R-HSA-1483257 Phospholipid metabolism R-HSA-6807878 COPI-mediated anterograde transport R-HSA-6811434 COPI-dependent Golgi-to-ER retrograde traffic R-HSA-164952 The role of Nef in HIV-1 replication and disease pathogenesis R-HSA-447115 Interleukin-12 family signaling R-HSA-168256 Immune System R-HSA-199991 Membrane Trafficking R-HSA-556833 Metabolism of lipids R-HSA-199977 ER to Golgi Anterograde Transport R-HSA-8856688 Golgi-to-ER retrograde transport R-HSA-162909 Host Interactions of HIV factors R-HSA-449147 Signaling by Interleukins R-HSA-5653656 Vesicle-mediated transport R-HSA-1430728 Metabolism R-HSA-948021 Transport to the Golgi and subsequent modification R-HSA-162906 HIV Infection R-HSA-1280215 Cytokine Signaling in Immune system R-HSA-446203 Asparagine N-linked glycosylation R-HSA-5663205 Infectious disease R-HSA-597592 Post-translational protein modification R-HSA-1643685 Disease R-HSA-392499 Metabolism of proteins
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Common Gene Haplotype Alleles 
