Human Gene ARHGEF4 (ENST00000409359.7_11) from GENCODE V47lift37
  Description: Rho guanine nucleotide exchange factor 4, transcript variant 4 (from RefSeq NM_001375900.1)
Gencode Transcript: ENST00000409359.7_11
Gencode Gene: ENSG00000136002.21_18
Transcript (Including UTRs)
   Position: hg19 chr2:131,594,487-131,804,826 Size: 210,340 Total Exon Count: 14 Strand: +
Coding Region
   Position: hg19 chr2:131,594,527-131,803,762 Size: 209,236 Coding Exon Count: 14 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr2:131,594,487-131,804,826)mRNA (may differ from genome)Protein (1876 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedUniProtKBWikipedia
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: E7EV07_HUMAN
DESCRIPTION: SubName: Full=Rho guanine nucleotide exchange factor 4;
CAUTION: The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 38.04 RPKM in Brain - Cortex
Total median expression: 437.09 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -18.0040-0.450 Picture PostScript Text
3' UTR -422.001064-0.397 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  Pfam Domains:
PF00018 - SH3 domain
PF00169 - PH domain
PF00621 - RhoGEF domain
PF07653 - Variant SH3 domain

SCOP Domains:
48065 - DBL homology domain (DH-domain)
50044 - SH3-domain
50729 - PH domain-like

ModBase Predicted Comparative 3D Structure on E7EV07
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details Gene Details  
Gene SorterGene Sorter Gene Sorter  
 RGDEnsembl   
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005085 guanyl-nucleotide exchange factor activity
GO:0005089 Rho guanyl-nucleotide exchange factor activity

Biological Process:
GO:0035023 regulation of Rho protein signal transduction
GO:0035556 intracellular signal transduction

Cellular Component:
GO:0005622 intracellular


-  Descriptions from all associated GenBank mRNAs
  AK127124 - Homo sapiens cDNA FLJ45181 fis, clone BRAWH3047644, highly similar to Homo sapiens Rho guanine nucleotide exchange factor (GEF) 4 (ARHGEF4).
AF249745 - Homo sapiens chromosome 2q22 RhoGEF mRNA, complete cds.
BC139742 - Homo sapiens Rho guanine nucleotide exchange factor (GEF) 4, mRNA (cDNA clone IMAGE:40035399), complete cds.
AB029035 - Homo sapiens KIAA1112 mRNA for KIAA1112 protein.
AB384116 - Synthetic construct DNA, clone: pF1KSDA1112, Homo sapiens ARHGEF4 gene for Rho guanine nucleotide exchange factor 4, complete cds, without stop codon, in Flexi system.
BC160189 - Synthetic construct Homo sapiens clone IMAGE:100064221, MGC:193304 Rho guanine nucleotide exchange factor (GEF) 4 (ARHGEF4) mRNA, encodes complete protein.
DQ424905 - Homo sapiens clone FG060308 rho guanine nucleotide exchange factor 4 variant 1 mRNA, complete cds, alternatively spliced.
JD324769 - Sequence 305793 from Patent EP1572962.
LF205178 - JP 2014500723-A/12681: Polycomb-Associated Non-Coding RNAs.
JD384582 - Sequence 365606 from Patent EP1572962.
JD338008 - Sequence 319032 from Patent EP1572962.
JD387218 - Sequence 368242 from Patent EP1572962.
JD260140 - Sequence 241164 from Patent EP1572962.
JD309535 - Sequence 290559 from Patent EP1572962.
JD090193 - Sequence 71217 from Patent EP1572962.
LF317291 - JP 2014500723-A/124794: Polycomb-Associated Non-Coding RNAs.
JD387405 - Sequence 368429 from Patent EP1572962.
JD367006 - Sequence 348030 from Patent EP1572962.
JD134920 - Sequence 115944 from Patent EP1572962.
JD495049 - Sequence 476073 from Patent EP1572962.
JD554852 - Sequence 535876 from Patent EP1572962.
JD297054 - Sequence 278078 from Patent EP1572962.
JD081110 - Sequence 62134 from Patent EP1572962.
JD067067 - Sequence 48091 from Patent EP1572962.
JD386787 - Sequence 367811 from Patent EP1572962.
JD169168 - Sequence 150192 from Patent EP1572962.
JD346024 - Sequence 327048 from Patent EP1572962.
JD215092 - Sequence 196116 from Patent EP1572962.
JD248428 - Sequence 229452 from Patent EP1572962.
JD332851 - Sequence 313875 from Patent EP1572962.
JD289648 - Sequence 270672 from Patent EP1572962.
JD286980 - Sequence 268004 from Patent EP1572962.
JD485567 - Sequence 466591 from Patent EP1572962.
JD157966 - Sequence 138990 from Patent EP1572962.
JD065395 - Sequence 46419 from Patent EP1572962.
JD373081 - Sequence 354105 from Patent EP1572962.
LF317292 - JP 2014500723-A/124795: Polycomb-Associated Non-Coding RNAs.
HZ473894 - WO 2016002844-A/8: ANTI-INVASIVE/ANTI-METASTATIC DRUG FOR PANCREATIC CANCER CELL.
JD440275 - Sequence 421299 from Patent EP1572962.
JD477898 - Sequence 458922 from Patent EP1572962.
JD436322 - Sequence 417346 from Patent EP1572962.
JD220987 - Sequence 202011 from Patent EP1572962.
JD523900 - Sequence 504924 from Patent EP1572962.
JD146059 - Sequence 127083 from Patent EP1572962.
JD383059 - Sequence 364083 from Patent EP1572962.
JD431682 - Sequence 412706 from Patent EP1572962.
JD114827 - Sequence 95851 from Patent EP1572962.
JD235683 - Sequence 216707 from Patent EP1572962.
JD350836 - Sequence 331860 from Patent EP1572962.
JD469091 - Sequence 450115 from Patent EP1572962.
JD336192 - Sequence 317216 from Patent EP1572962.
JD050310 - Sequence 31334 from Patent EP1572962.
JD371667 - Sequence 352691 from Patent EP1572962.
JD502388 - Sequence 483412 from Patent EP1572962.
JD380607 - Sequence 361631 from Patent EP1572962.
JD332694 - Sequence 313718 from Patent EP1572962.
JD177193 - Sequence 158217 from Patent EP1572962.
JD165456 - Sequence 146480 from Patent EP1572962.
MA440755 - JP 2018138019-A/12681: Polycomb-Associated Non-Coding RNAs.
MA552868 - JP 2018138019-A/124794: Polycomb-Associated Non-Coding RNAs.
MA552869 - JP 2018138019-A/124795: Polycomb-Associated Non-Coding RNAs.
MB485431 - JP 2019206516-A/4: ANTI-INVASIVE/ANTI-METASTATIC DRUG FOR PANCREATIC CANCER CELL.
AB042199 - Homo sapiens Asef mRNA for APC-stimulated guanine nucleotide exchange factor, complete cds.
AL137289 - Homo sapiens mRNA; cDNA DKFZp434G2016 (from clone DKFZp434G2016).
BC036579 - Homo sapiens Rho guanine nucleotide exchange factor (GEF) 4, mRNA (cDNA clone IMAGE:4792809), with apparent retained intron.
HZ473892 - WO 2016002844-A/6: ANTI-INVASIVE/ANTI-METASTATIC DRUG FOR PANCREATIC CANCER CELL.
HZ473893 - WO 2016002844-A/7: ANTI-INVASIVE/ANTI-METASTATIC DRUG FOR PANCREATIC CANCER CELL.
JD161915 - Sequence 142939 from Patent EP1572962.
JD453562 - Sequence 434586 from Patent EP1572962.
JD395234 - Sequence 376258 from Patent EP1572962.
JD139681 - Sequence 120705 from Patent EP1572962.
JD060836 - Sequence 41860 from Patent EP1572962.
JD386623 - Sequence 367647 from Patent EP1572962.
JD325360 - Sequence 306384 from Patent EP1572962.
JD477455 - Sequence 458479 from Patent EP1572962.
JD164053 - Sequence 145077 from Patent EP1572962.
JD329018 - Sequence 310042 from Patent EP1572962.
JD129116 - Sequence 110140 from Patent EP1572962.
JD165480 - Sequence 146504 from Patent EP1572962.
JD190829 - Sequence 171853 from Patent EP1572962.
JD414306 - Sequence 395330 from Patent EP1572962.
JD423327 - Sequence 404351 from Patent EP1572962.
JD372800 - Sequence 353824 from Patent EP1572962.
JD282819 - Sequence 263843 from Patent EP1572962.
JD112812 - Sequence 93836 from Patent EP1572962.
JD500704 - Sequence 481728 from Patent EP1572962.
JD118693 - Sequence 99717 from Patent EP1572962.
JD114786 - Sequence 95810 from Patent EP1572962.
JD063747 - Sequence 44771 from Patent EP1572962.
JD515822 - Sequence 496846 from Patent EP1572962.
JD493951 - Sequence 474975 from Patent EP1572962.
JD110011 - Sequence 91035 from Patent EP1572962.
JD320654 - Sequence 301678 from Patent EP1572962.
JD381115 - Sequence 362139 from Patent EP1572962.
JD442687 - Sequence 423711 from Patent EP1572962.
JD335129 - Sequence 316153 from Patent EP1572962.
HZ473891 - WO 2016002844-A/5: ANTI-INVASIVE/ANTI-METASTATIC DRUG FOR PANCREATIC CANCER CELL.
MB485429 - JP 2019206516-A/2: ANTI-INVASIVE/ANTI-METASTATIC DRUG FOR PANCREATIC CANCER CELL.
MB485430 - JP 2019206516-A/3: ANTI-INVASIVE/ANTI-METASTATIC DRUG FOR PANCREATIC CANCER CELL.
MB485428 - JP 2019206516-A/1: ANTI-INVASIVE/ANTI-METASTATIC DRUG FOR PANCREATIC CANCER CELL.

-  Other Names for This Gene
  Alternate Gene Symbols: ARHGEF4 , E7EV07, E7EV07_HUMAN, ENST00000409359.1, ENST00000409359.2, ENST00000409359.3, ENST00000409359.4, ENST00000409359.5, ENST00000409359.6, NM_001375900, uc319fpt.1, uc319fpt.2
UCSC ID: ENST00000409359.7_11
RefSeq Accession: NM_001367493.1
Protein: E7EV07

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.