Human Gene ARL8B (ENST00000256496.8_4) from GENCODE V47lift37
  Description: ADP ribosylation factor like GTPase 8B (from RefSeq NM_018184.3)
Gencode Transcript: ENST00000256496.8_4
Gencode Gene: ENSG00000134108.14_7
Transcript (Including UTRs)
   Position: hg19 chr3:5,163,977-5,222,596 Size: 58,620 Total Exon Count: 7 Strand: +
Coding Region
   Position: hg19 chr3:5,164,151-5,220,398 Size: 56,248 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr3:5,163,977-5,222,596)mRNA (may differ from genome)Protein (186 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedUniProtKBWikipedia
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: ARL8B_HUMAN
DESCRIPTION: RecName: Full=ADP-ribosylation factor-like protein 8B; AltName: Full=ADP-ribosylation factor-like protein 10C; AltName: Full=Novel small G protein indispensable for equal chromosome segregation 1;
FUNCTION: May play a role in lysosome motility (PubMed:16537643). May play a role in chromosome segregation (PubMed:15331635).
SUBUNIT: Interacts with tubulin.
SUBCELLULAR LOCATION: Late endosome membrane. Lysosome membrane. Cytoplasm, cytoskeleton, spindle. Note=According to PubMed:15331635, it localizes with microtubules at the spindle mid-zone during mitosis.
TISSUE SPECIFICITY: Ubiquitously expressed.
SIMILARITY: Belongs to the small GTPase superfamily. Arf family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene
  • D013749 Tetrachlorodibenzodioxin
  • C532162 2-(1H-indazol-4-yl)-6-(4-methanesulfonylpiperazin-1-ylmethyl)-4-morpholin-4-ylthieno(3,2-d)pyrimidine
  • C009505 4,4'-diaminodiphenylmethane
  • D015123 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide
  • D015127 9,10-Dimethyl-1,2-benzanthracene
  • D000082 Acetaminophen
  • D002994 Clofibrate
  • D003300 Copper
  • D019327 Copper Sulfate
  • D016572 Cyclosporine
          more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 55.83 RPKM in Brain - Frontal Cortex (BA9)
Total median expression: 1588.33 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -74.40174-0.428 Picture PostScript Text
3' UTR -538.102198-0.245 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR005225 - Small_GTP-bd_dom
IPR024156 - Small_GTPase_ARF
IPR006689 - Small_GTPase_ARF/SAR

Pfam Domains:
PF00025 - ADP-ribosylation factor family
PF00071 - Ras family
PF01926 - 50S ribosome-binding GTPase
PF04670 - Gtr1/RagA G protein conserved region
PF08477 - Ras of Complex, Roc, domain of DAPkinase
PF09439 - Signal recognition particle receptor beta subunit

SCOP Domains:
52540 - P-loop containing nucleoside triphosphate hydrolases

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2AL7 - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q9NVJ2
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologGenome BrowserNo ortholog
Gene DetailsGene Details Gene DetailsGene Details 
Gene SorterGene Sorter Gene SorterGene Sorter 
 RGDEnsembl WormBase 
    Protein Sequence 
    Alignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0003924 GTPase activity
GO:0005515 protein binding
GO:0005525 GTP binding
GO:0019003 GDP binding
GO:0043014 alpha-tubulin binding
GO:0048487 beta-tubulin binding

Biological Process:
GO:0007049 cell cycle
GO:0007059 chromosome segregation
GO:0032418 lysosome localization
GO:0051301 cell division

Cellular Component:
GO:0005622 intracellular
GO:0005737 cytoplasm
GO:0005764 lysosome
GO:0005765 lysosomal membrane
GO:0005768 endosome
GO:0005819 spindle
GO:0005856 cytoskeleton
GO:0016020 membrane
GO:0030496 midbody
GO:0031902 late endosome membrane
GO:0051233 spindle midzone
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  DL492418 - Novel nucleic acids.
BC063125 - Homo sapiens ADP-ribosylation factor-like 8B, mRNA (cDNA clone MGC:71988 IMAGE:6622791), complete cds.
AK303872 - Homo sapiens cDNA FLJ56285 complete cds, highly similar to ADP-ribosylation factor-like protein 8B.
AK298951 - Homo sapiens cDNA FLJ61158 complete cds, highly similar to ADP-ribosylation factor-like protein 8B.
AK001564 - Homo sapiens cDNA FLJ10702 fis, clone NT2RP3000759, weakly similar to ADP-RIBOSYLATION FACTOR.
AK295465 - Homo sapiens cDNA FLJ50155 complete cds, highly similar to ADP-ribosylation factor-like protein 8B.
BC013131 - Homo sapiens ADP-ribosylation factor-like 8B, mRNA (cDNA clone MGC:21136 IMAGE:4508077), complete cds.
DL490898 - Novel nucleic acids.
JD544886 - Sequence 525910 from Patent EP1572962.
JD500760 - Sequence 481784 from Patent EP1572962.
AB528951 - Synthetic construct DNA, clone: pF1KB6820, Homo sapiens ARL8B gene for ADP-ribosylation factor-like 8B, without stop codon, in Flexi system.
CR457264 - Homo sapiens full open reading frame cDNA clone RZPDo834E019D for gene ARL10C, ADP-ribosylation factor-like 10C; complete cds, incl. stopcodon.
AB118751 - Homo sapiens gie1 mRNA, complete cds.
JD287712 - Sequence 268736 from Patent EP1572962.
JD358526 - Sequence 339550 from Patent EP1572962.
AF086108 - Homo sapiens full length insert cDNA clone YZ99E06.
JD067785 - Sequence 48809 from Patent EP1572962.
JD081829 - Sequence 62853 from Patent EP1572962.
JD525235 - Sequence 506259 from Patent EP1572962.
JD254823 - Sequence 235847 from Patent EP1572962.
JD375114 - Sequence 356138 from Patent EP1572962.
JD175149 - Sequence 156173 from Patent EP1572962.
AL110242 - Homo sapiens mRNA; cDNA DKFZp566L034 (from clone DKFZp566L034).
JD548864 - Sequence 529888 from Patent EP1572962.
JD531183 - Sequence 512207 from Patent EP1572962.
JD418016 - Sequence 399040 from Patent EP1572962.
JD299027 - Sequence 280051 from Patent EP1572962.
JD411588 - Sequence 392612 from Patent EP1572962.
JD172095 - Sequence 153119 from Patent EP1572962.
JD233364 - Sequence 214388 from Patent EP1572962.
JD548693 - Sequence 529717 from Patent EP1572962.
JD084524 - Sequence 65548 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: ARL10C, ARL8B , ARL8B_HUMAN, B4DI85, ENST00000256496.1, ENST00000256496.2, ENST00000256496.3, ENST00000256496.4, ENST00000256496.5, ENST00000256496.6, ENST00000256496.7, GIE1, NM_018184, Q9NVJ2, uc317fva.1, uc317fva.2
UCSC ID: ENST00000256496.8_4
RefSeq Accession: NM_018184.3
Protein: Q9NVJ2 (aka ARL8B_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.