Human Gene ATP5ME (ENST00000304312.5_7) from GENCODE V47lift37
  Description: ATP synthase membrane subunit e, transcript variant 1 (from RefSeq NM_007100.4)
Gencode Transcript: ENST00000304312.5_7
Gencode Gene: ENSG00000169020.10_10
Transcript (Including UTRs)
   Position: hg19 chr4:666,225-668,065 Size: 1,841 Total Exon Count: 4 Strand: -
Coding Region
   Position: hg19 chr4:666,289-668,036 Size: 1,748 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersGene AllelesRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr4:666,225-668,065)mRNA (may differ from genome)Protein (69 aa)
Gene SorterGenome BrowserOther Species FASTATable SchemaAlphaFoldBioGPS
EnsemblEntrez GeneExonPrimerGeneCardsMGIOMIM
PubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: ATP5I_HUMAN
DESCRIPTION: RecName: Full=ATP synthase subunit e, mitochondrial; Short=ATPase subunit e;
FUNCTION: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane.
SUBUNIT: F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(0) seems to have nine subunits: a, b, c, d, e, f, g, F6 and 8 (or A6L). Component of an ATP synthase complex composed of ATP5F1, ATP5G1, ATP5E, ATP5H, ATP5I, ATP5J, ATP5J2, MT-ATP6, MT-ATP8, ATP5A1, ATP5B, ATP5D, ATP5C1, ATP5O, ATP5L, USMG5 and MP68 (By similarity).
SUBCELLULAR LOCATION: Mitochondrion. Mitochondrion inner membrane.
SIMILARITY: Belongs to the ATPase e subunit family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 299.64 RPKM in Heart - Left Ventricle
Total median expression: 6715.68 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -7.9029-0.272 Picture PostScript Text
3' UTR -11.1064-0.173 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR008386 - ATPase_F0-cplx_esu_mt

Pfam Domains:
PF05680 - ATP synthase E chain

ModBase Predicted Comparative 3D Structure on P56385
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details Gene Details  
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0015078 hydrogen ion transmembrane transporter activity
GO:0022857 transmembrane transporter activity
GO:0016887 ATPase activity

Biological Process:
GO:0006754 ATP biosynthetic process
GO:0006811 ion transport
GO:0015986 ATP synthesis coupled proton transport
GO:0042407 cristae formation
GO:0042776 mitochondrial ATP synthesis coupled proton transport
GO:0046034 ATP metabolic process

Cellular Component:
GO:0000276 mitochondrial proton-transporting ATP synthase complex, coupling factor F(o)
GO:0005739 mitochondrion
GO:0005743 mitochondrial inner membrane
GO:0005753 mitochondrial proton-transporting ATP synthase complex
GO:0016020 membrane
GO:0045263 proton-transporting ATP synthase complex, coupling factor F(o)


-  Descriptions from all associated GenBank mRNAs
  AK000656 - Homo sapiens cDNA FLJ20649 fis, clone KAT02037.
AB028624 - Homo sapiens mRNA for mitochondrial ATP Synthase subunit e, partial cds.
D50371 - Homo sapiens mRNA for ATP synthase subunit e, complete cds.
BC017215 - Homo sapiens ATP synthase, H+ transporting, mitochondrial F0 complex, subunit E, mRNA (cDNA clone IMAGE:4079694).
BC003679 - Homo sapiens ATP synthase, H+ transporting, mitochondrial F0 complex, subunit E, mRNA (cDNA clone MGC:12532 IMAGE:4052832), complete cds.
BC105610 - Homo sapiens ATP synthase, H+ transporting, mitochondrial F0 complex, subunit E, mRNA (cDNA clone MGC:104384 IMAGE:6739801), complete cds.
JD332648 - Sequence 313672 from Patent EP1572962.
KJ890738 - Synthetic construct Homo sapiens clone ccsbBroadEn_00132 ATP5I gene, encodes complete protein.
BT006922 - Homo sapiens ATP synthase, H+ transporting, mitochondrial F0 complex, subunit e mRNA, complete cds.
JD344837 - Sequence 325861 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  BioCyc Knowledge Library
PWY-7980 - ATP biosynthesis

Reactome (by CSHL, EBI, and GO)

Protein P56385 (Reactome details) participates in the following event(s):

R-HSA-164840 ADP and Pi bind to ATPase
R-HSA-8949580 F1Fo ATP synthase dimerizes
R-HSA-164834 Enzyme-bound ATP is released
R-HSA-164832 ATPase synthesizes ATP
R-HSA-163210 Formation of ATP by chemiosmotic coupling
R-HSA-8949613 Cristae formation
R-HSA-163200 Respiratory electron transport, ATP synthesis by chemiosmotic coupling, and heat production by uncoupling proteins.
R-HSA-1592230 Mitochondrial biogenesis
R-HSA-1428517 The citric acid (TCA) cycle and respiratory electron transport
R-HSA-1852241 Organelle biogenesis and maintenance
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: ATP5I , ATP5I_HUMAN, ATP5K, ATP5ME , ENST00000304312.1, ENST00000304312.2, ENST00000304312.3, ENST00000304312.4, NM_007100, P56385, Q0D2L9, uc317nkb.1, uc317nkb.2
UCSC ID: ENST00000304312.5_7
RefSeq Accession: NM_007100.4
Protein: P56385 (aka ATP5I_HUMAN or ATPJ_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.