ID:GO45_HUMAN DESCRIPTION: RecName: Full=Golgin-45; AltName: Full=Basic leucine zipper nuclear factor 1; AltName: Full=JEM-1; AltName: Full=p45 basic leucine-zipper nuclear factor; FUNCTION: Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface. SUBUNIT: Interacts with GORASP2 and with the GTP-bound form of RAB2, but not with other Golgi Rab proteins. GORASP2 and BLZF1 form a RAB2 effector complex on medial Golgi. SUBCELLULAR LOCATION: Golgi apparatus lumen. SUBCELLULAR LOCATION: Isoform 1: Nucleus. SUBCELLULAR LOCATION: Isoform 2: Cytoplasm. TISSUE SPECIFICITY: Ubiquitous. Also found in cell lines derived from several hematopoietic pathologies, such as T-cell leukemia, pro-B, pre-B, myeloma, and plasmacytoma cell lines, but not in Burkitt lymphoma cells. INDUCTION: Up-regulated by retinoids. DOMAIN: The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2. PTM: ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP- ribosylated protein is recognized by RNF146, followed by ubiquitination. PTM: Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation. CAUTION: Because of the presence of a potential basic motif and leucine-zipper domain, PubMed:9129147 and PubMed:11056056 have thought that BLZF1 is a potential transcription factor. They found it localized in the nucleus, except isoform 2, which was cytoplasmic. However, homology at several typical position for basic or hydrophobic residues is missing.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
Pfam Domains: PF08227 - DASH complex subunit Hsk3 like
ModBase Predicted Comparative 3D Structure on Q9H2G9
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Gene Ontology (GO) Annotations with Structured Vocabulary
Molecular Function: GO:0003677 DNA binding GO:0003700 transcription factor activity, sequence-specific DNA binding GO:0005515 protein binding GO:0019899 enzyme binding GO:0031625 ubiquitin protein ligase binding
Biological Process: GO:0001558 regulation of cell growth GO:0006357 regulation of transcription from RNA polymerase II promoter GO:0007030 Golgi organization GO:0008283 cell proliferation GO:0015031 protein transport GO:0016192 vesicle-mediated transport GO:0043001 Golgi to plasma membrane protein transport
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
