Human Gene BROX (ENST00000340934.10_4) from GENCODE V47lift37
  Description: BRO1 domain and CAAX motif containing, transcript variant 1 (from RefSeq NM_144695.4)
Gencode Transcript: ENST00000340934.10_4
Gencode Gene: ENSG00000162819.12_10
Transcript (Including UTRs)
   Position: hg19 chr1:222,885,906-222,908,529 Size: 22,624 Total Exon Count: 13 Strand: +
Coding Region
   Position: hg19 chr1:222,889,042-222,906,056 Size: 17,015 Coding Exon Count: 12 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:222,885,906-222,908,529)mRNA (may differ from genome)Protein (411 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIPubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: BROX_HUMAN
DESCRIPTION: RecName: Full=BRO1 domain-containing protein BROX; AltName: Full=BRO1 domain- and CAAX motif-containing protein; Flags: Precursor;
SUBUNIT: Interacts with CHMP4B.
SUBCELLULAR LOCATION: Membrane; Lipid-anchor (Potential).
SIMILARITY: Belongs to the BROX family.
SIMILARITY: Contains 1 BRO1 domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 11.57 RPKM in Small Intestine - Terminal Ileum
Total median expression: 396.49 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -133.00395-0.337 Picture PostScript Text
3' UTR -569.502473-0.230 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR004328 - BRO1_dom

Pfam Domains:
PF03097 - BRO1-like domain

Protein Data Bank (PDB) 3-D Structure
MuPIT help
3R9M - X-ray MuPIT 3ULY - X-ray MuPIT 3UM0 - X-ray MuPIT 3UM1 - X-ray MuPIT 3UM2 - X-ray MuPIT 3UM3 - X-ray MuPIT 3ZXP - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q5VW32
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologGenome BrowserNo ortholog
Gene DetailsGene Details  Gene Details 
Gene SorterGene Sorter  Gene Sorter 
 RGDEnsembl WormBase 
    Protein Sequence 
    Alignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding

Cellular Component:
GO:0016020 membrane
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  AK056983 - Homo sapiens cDNA FLJ32421 fis, clone SKMUS2000902, weakly similar to BRO1 PROTEIN.
JD523527 - Sequence 504551 from Patent EP1572962.
LF370974 - JP 2014500723-A/178477: Polycomb-Associated Non-Coding RNAs.
BC053552 - Homo sapiens cDNA clone IMAGE:6092659, partial cds.
BC113637 - Homo sapiens chromosome 1 open reading frame 58, mRNA (cDNA clone MGC:142197 IMAGE:8322689), complete cds.
BC113635 - Homo sapiens chromosome 1 open reading frame 58, mRNA (cDNA clone MGC:142195 IMAGE:8322687), complete cds.
BC143811 - Homo sapiens chromosome 1 open reading frame 58, mRNA (cDNA clone MGC:177343 IMAGE:9052326), complete cds.
BC105293 - Homo sapiens cDNA clone IMAGE:6725901.
JD325033 - Sequence 306057 from Patent EP1572962.
JD350448 - Sequence 331472 from Patent EP1572962.
JD325682 - Sequence 306706 from Patent EP1572962.
AK304445 - Homo sapiens cDNA FLJ53758 complete cds.
AK315930 - Homo sapiens cDNA, FLJ78829 complete cds.
AK293974 - Homo sapiens cDNA FLJ50123 complete cds.
HQ258409 - Synthetic construct Homo sapiens clone IMAGE:100072838 chromosome 1 open reading frame 58 (C1orf58) gene, encodes complete protein.
KJ895619 - Synthetic construct Homo sapiens clone ccsbBroadEn_05013 BROX gene, encodes complete protein.
AB276123 - Homo sapiens Brox mRNA for Bro1-domain-containing protein, complete cds.
JD095810 - Sequence 76834 from Patent EP1572962.
AK098136 - Homo sapiens cDNA FLJ40817 fis, clone TRACH2010639.
JD206175 - Sequence 187199 from Patent EP1572962.
AK025140 - Homo sapiens cDNA: FLJ21487 fis, clone COL05419.
MA606551 - JP 2018138019-A/178477: Polycomb-Associated Non-Coding RNAs.

-  Other Names for This Gene
  Alternate Gene Symbols: B7Z9G5, BROFTI, BROX , BROX_HUMAN, C1orf58, ENST00000340934.1, ENST00000340934.2, ENST00000340934.3, ENST00000340934.4, ENST00000340934.5, ENST00000340934.6, ENST00000340934.7, ENST00000340934.8, ENST00000340934.9, NM_144695, Q5VW32, Q96MG1, uc317vvx.1, uc317vvx.2
UCSC ID: ENST00000340934.10_4
RefSeq Accession: NM_144695.4
Protein: Q5VW32 (aka BROX_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.