ID:BUB3_HUMAN DESCRIPTION: RecName: Full=Mitotic checkpoint protein BUB3; FUNCTION: Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. SUBUNIT: Interacts with BUB1 and BUBR1. The BUB1/BUB3 complex interacts with MAD1L1. INTERACTION: Q5XI90:Dynlt3 (xeno); NbExp=2; IntAct=EBI-1050987, EBI-1781818; SUBCELLULAR LOCATION: Nucleus. Chromosome, centromere, kinetochore (By similarity). Note=Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition (By similarity). PTM: Poly-ADP-ribosylated by PARP1 (By similarity). SIMILARITY: Belongs to the WD repeat BUB3 family. SIMILARITY: Contains 5 WD repeats.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on O43684
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
BX647392 - Homo sapiens mRNA; cDNA DKFZp686J24144 (from clone DKFZp686J24144). AK312546 - Homo sapiens cDNA, FLJ92917, Homo sapiens BUB3 budding uninhibited by benzimidazoles 3 homolog(yeast) (BUB3), mRNA. AK307958 - Homo sapiens cDNA, FLJ97906. AK293255 - Homo sapiens cDNA FLJ57899 complete cds, highly similar to Mitotic checkpoint protein BUB3. AF081496 - Homo sapiens kinetochore protein BUB3 (BUB3) mRNA, complete cds. BC005138 - Homo sapiens budding uninhibited by benzimidazoles 3 homolog (yeast), mRNA (cDNA clone MGC:1527 IMAGE:3504520), complete cds. AF047472 - Homo sapiens spleen mitotic checkpoint BUB3 (BUB3) mRNA, complete cds. AF047473 - Homo sapiens testis mitotic checkpoint BUB3 (BUB3) mRNA, complete cds. BC022438 - Homo sapiens budding uninhibited by benzimidazoles 3 homolog (yeast), mRNA (cDNA clone MGC:24748 IMAGE:4281970), complete cds. AK226060 - Homo sapiens mRNA for BUB3 budding uninhibited by benzimidazoles 3 isoform a variant, clone: hg01710. AF053304 - Homo sapiens mitotic checkpoint component Bub3 (BUB3) mRNA, complete cds. HM005340 - Homo sapiens clone HTL-T-27 testicular tissue protein Li 27 mRNA, complete cds. CU692616 - Synthetic construct Homo sapiens gateway clone IMAGE:100022217 5' read BUB3 mRNA. AB463452 - Synthetic construct DNA, clone: pF1KB9385, Homo sapiens BUB3 gene for BUB3 budding uninhibited by benzimidazoles 3 homolog, without stop codon, in Flexi system. AB590162 - Synthetic construct DNA, clone: pFN21AE1557, Homo sapiens BUB3 gene for budding uninhibited by benzimidazoles 3 homolog, without stop codon, in Flexi system. DQ891852 - Synthetic construct clone IMAGE:100004482; FLH180614.01X; RZPDo839E02134D BUB3 budding uninhibited by benzimidazoles 3 homolog (yeast) (BUB3) gene, encodes complete protein. DQ895042 - Synthetic construct Homo sapiens clone IMAGE:100009502; FLH180610.01L; RZPDo839E02133D BUB3 budding uninhibited by benzimidazoles 3 homolog (yeast) (BUB3) gene, encodes complete protein. KJ892706 - Synthetic construct Homo sapiens clone ccsbBroadEn_02100 BUB3 gene, encodes complete protein. KJ897977 - Synthetic construct Homo sapiens clone ccsbBroadEn_07371 BUB3 gene, encodes complete protein. JD165124 - Sequence 146148 from Patent EP1572962. JD304942 - Sequence 285966 from Patent EP1572962. JD488945 - Sequence 469969 from Patent EP1572962. JD504011 - Sequence 485035 from Patent EP1572962. JD358229 - Sequence 339253 from Patent EP1572962. JD310900 - Sequence 291924 from Patent EP1572962. JD352191 - Sequence 333215 from Patent EP1572962. JD290514 - Sequence 271538 from Patent EP1572962. JD296806 - Sequence 277830 from Patent EP1572962. JD491254 - Sequence 472278 from Patent EP1572962. JD335551 - Sequence 316575 from Patent EP1572962. JD308284 - Sequence 289308 from Patent EP1572962. JD127190 - Sequence 108214 from Patent EP1572962.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein O43684 (Reactome details) participates in the following event(s):
R-HSA-141437 Formation of the MCC complex R-HSA-141423 Binding of the MCC complex to the APC/C complex R-HSA-174238 Activation of APC/C:Cdc20 by dissociation of Cdc20:phospho-APC/C from Cdc20:phospho-APC/C:Mad2:Bub3:BubR1 R-HSA-174171 Association of Cyclin A with the APC/C R-HSA-179410 Association of Nek2A with MCC:APC/C R-HSA-141409 Mad1 binds kinetochore R-HSA-375302 Kinetochore capture of astral microtubules R-HSA-5666129 CDC42:GTP recruits DIAPH2-2 to kinetochores R-HSA-5666169 Kinetochore capture of astral microtubules is positively regulated by CDC42:GTP:p-S196-DIAPH2-2 R-HSA-174104 Ubiquitination of Cyclin A by APC/C:Cdc20 complex R-HSA-179417 Multiubiquitination of Nek2A R-HSA-141431 MAD2 associates with the Mad1 kinetochore complex R-HSA-141439 Release of activated MAD2 from kinetochores R-HSA-2467811 Separation of sister chromatids R-HSA-2467809 ESPL1 (Separase) cleaves centromeric cohesin R-HSA-5666160 AURKB phosphorylates DIAPH2-2 at kinetochores R-HSA-141422 MAD2 converted to an inhibitory state via interaction with Mad1 R-HSA-1638821 PP2A-B56 dephosphorylates centromeric cohesin R-HSA-1638803 Phosphorylation of cohesin by PLK1 at centromeres R-HSA-2468287 CDK1 phosphorylates CDCA5 (Sororin) at centromeres R-HSA-141430 Inactivation of APC/C via direct inhibition of the APC/C complex R-HSA-176409 APC/C:Cdc20 mediated degradation of mitotic proteins R-HSA-141405 Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components R-HSA-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-HSA-179409 APC-Cdc20 mediated degradation of Nek2A R-HSA-176814 Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins R-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-HSA-68877 Mitotic Prometaphase R-HSA-5663220 RHO GTPases Activate Formins R-HSA-69618 Mitotic Spindle Checkpoint R-HSA-176408 Regulation of APC/C activators between G1/S and early anaphase R-HSA-179419 APC:Cdc20 mediated degradation of cell cycle proteins prior to satisfation of the cell cycle checkpoint R-HSA-174143 APC/C-mediated degradation of cell cycle proteins R-HSA-2500257 Resolution of Sister Chromatid Cohesion R-HSA-2467813 Separation of Sister Chromatids R-HSA-141424 Amplification of signal from the kinetochores R-HSA-68886 M Phase R-HSA-195258 RHO GTPase Effectors R-HSA-69620 Cell Cycle Checkpoints R-HSA-453276 Regulation of mitotic cell cycle R-HSA-68882 Mitotic Anaphase R-HSA-69278 Cell Cycle (Mitotic) R-HSA-194315 Signaling by Rho GTPases R-HSA-1640170 Cell Cycle R-HSA-2555396 Mitotic Metaphase and Anaphase R-HSA-162582 Signal Transduction
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
