Human Gene BUD31 (ENST00000222969.10_4) from GENCODE V47lift37
  Description: BUD31 homolog, transcript variant 1 (from RefSeq NM_003910.4)
Gencode Transcript: ENST00000222969.10_4
Gencode Gene: ENSG00000106245.11_8
Transcript (Including UTRs)
   Position: hg19 chr7:99,006,592-99,017,239 Size: 10,648 Total Exon Count: 6 Strand: +
Coding Region
   Position: hg19 chr7:99,008,716-99,017,064 Size: 8,349 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr7:99,006,592-99,017,239)mRNA (may differ from genome)Protein (144 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsHGNC
MGIOMIMPubMedReactomeUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: BUD31_HUMAN
DESCRIPTION: RecName: Full=Protein BUD31 homolog; AltName: Full=Protein EDG-2; AltName: Full=Protein G10 homolog;
SUBCELLULAR LOCATION: Nucleus (Potential).
SIMILARITY: Belongs to the BUD31 (G10) family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene
  • D013749 Tetrachlorodibenzodioxin
  • C517041 (4-amino-1,4-dihydro-3-(2-pyridyl)-5-thioxo-1,2,4-triazole)copper(II)
  • C111118 2',3,3',4',5-pentachloro-4-hydroxybiphenyl
  • C014211 2,3,7,8-tetrachlorodibenzofuran
  • C016403 2,4-dinitrotoluene
  • C023514 2,6-dinitrotoluene
  • C009505 4,4'-diaminodiphenylmethane
  • D000643 Ammonium Chloride
  • D001564 Benzo(a)pyrene
  • D019327 Copper Sulfate
          more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 51.81 RPKM in Testis
Total median expression: 1283.20 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -168.10442-0.380 Picture PostScript Text
3' UTR -54.10175-0.309 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR018230 - BUD31/G10-rel_CS
IPR001748 - G10

Pfam Domains:
PF01125 - G10 protein

ModBase Predicted Comparative 3D Structure on P41223
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologGenome Browser
Gene DetailsGene Details Gene Details Gene Details
Gene SorterGene Sorter Gene Sorter Gene Sorter
 RGDEnsembl  SGD
     Protein Sequence
     Alignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003700 transcription factor activity, sequence-specific DNA binding
GO:0005515 protein binding
GO:0030374 ligand-dependent nuclear receptor transcription coactivator activity
GO:0035257 nuclear hormone receptor binding

Biological Process:
GO:0000398 mRNA splicing, via spliceosome
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006357 regulation of transcription from RNA polymerase II promoter
GO:0006397 mRNA processing
GO:0008380 RNA splicing
GO:2000825 positive regulation of androgen receptor activity

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005681 spliceosomal complex
GO:0071007 U2-type catalytic step 2 spliceosome
GO:0000790 nuclear chromatin


-  Descriptions from all associated GenBank mRNAs
  AK297784 - Homo sapiens cDNA FLJ53814 complete cds, highly similar to Protein G10 homolog.
BC104670 - Homo sapiens BUD31 homolog (S. cerevisiae), mRNA (cDNA clone MGC:111202 IMAGE:30318048), complete cds.
AK316477 - Homo sapiens cDNA, FLJ79376 complete cds, highly similar to Protein G10 homolog.
BC022821 - Homo sapiens BUD31 homolog (S. cerevisiae), mRNA (cDNA clone MGC:39264 IMAGE:5087938), complete cds.
S77329 - edg-2=maternal transcript G10 homolog [human, umbilical vein endothelial cells, mRNA, 1003 nt].
U11861 - Human G10 homolog (edg-2) mRNA, complete cds.
JD266887 - Sequence 247911 from Patent EP1572962.
JD419053 - Sequence 400077 from Patent EP1572962.
CR456951 - Homo sapiens full open reading frame cDNA clone RZPDo834D0516D for gene G10, maternal G10 transcript; complete cds, incl. stopcodon.
AB463453 - Synthetic construct DNA, clone: pF1KB7523, Homo sapiens BUD31 gene for BUD31 homolog, without stop codon, in Flexi system.
CU692726 - Synthetic construct Homo sapiens gateway clone IMAGE:100021852 5' read BUD31 mRNA.
KJ892651 - Synthetic construct Homo sapiens clone ccsbBroadEn_02045 BUD31 gene, encodes complete protein.
JD344583 - Sequence 325607 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein P41223 (Reactome details) participates in the following event(s):

R-HSA-72127 Formation of the Spliceosomal B Complex
R-HSA-72143 Lariat Formation and 5'-Splice Site Cleavage
R-HSA-72139 Formation of the active Spliceosomal C (B*) complex
R-HSA-72130 Formation of an intermediate Spliceosomal C (Bact) complex
R-HSA-156661 Formation of Exon Junction Complex
R-HSA-72163 mRNA Splicing - Major Pathway
R-HSA-72172 mRNA Splicing
R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA
R-HSA-8953854 Metabolism of RNA

-  Other Names for This Gene
  Alternate Gene Symbols: A4D274, B7Z4S9, BUD31_HUMAN, D6W5S6, EDG2, ENST00000222969.1, ENST00000222969.2, ENST00000222969.3, ENST00000222969.4, ENST00000222969.5, ENST00000222969.6, ENST00000222969.7, ENST00000222969.8, ENST00000222969.9, NM_003910, P41223, Q6IB53, Q9UDV1, uc317dga.1, uc317dga.2
UCSC ID: ENST00000222969.10_4
RefSeq Accession: NM_003910.4
Protein: P41223 (aka BUD31_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.