Human Gene AOPEP (ENST00000277198.6_1) from GENCODE V47lift37
  Description: aminopeptidase O (putative), transcript variant 15 (from RefSeq NM_001386073.1)
Gencode Transcript: ENST00000277198.6_1
Gencode Gene: ENSG00000148120.19_9
Transcript (Including UTRs)
   Position: hg19 chr9:97,488,983-97,694,115 Size: 205,133 Total Exon Count: 8 Strand: +
Coding Region
   Position: hg19 chr9:97,522,066-97,694,036 Size: 171,971 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersGene AllelesRNA-Seq Expression
RNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathways
Other NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr9:97,488,983-97,694,115)mRNA (may differ from genome)Protein (566 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblExonPrimerGeneCardsMGIPubMed
ReactomeUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: AMPO_HUMAN
DESCRIPTION: RecName: Full=Aminopeptidase O; Short=AP-O; EC=3.4.11.-;
FUNCTION: Aminopeptidases catalyze the hydrolysis of amino acid residues from the N-terminus of peptide or protein substrates. Able to cleave angiotensin III to generate angiotensin IV, a bioactive peptide of the renin-angiotensin pathway. Not able to cleave angiotensin I and angiotensin II. May play a role in the proteolytic processing of bioactive peptides in tissues such as testis and heart.
COFACTOR: Binds 1 zinc ion per subunit (By similarity).
ENZYME REGULATION: Strongly inhibited by ophenanthroline, a metalloprotease inhibitor. Inhibited by arphamenine A, a potent inhibitor of aminopeptidases. Not affected by AEBSF and E-64, which are common inhibitors of serine- and cysteine-proteases, respectively.
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=6 uM for Asn-AMC;
SUBCELLULAR LOCATION: Cytoplasm (Potential).
TISSUE SPECIFICITY: Predominantly expressed in pancreas, placenta, liver, testis and heart. Expressed at lower level in brain, lung and kidney.
SIMILARITY: Belongs to the peptidase M1 family.
SEQUENCE CAUTION: Sequence=AAH20194.1; Type=Erroneous initiation; Sequence=BAB55210.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 13.85 RPKM in Artery - Aorta
Total median expression: 261.57 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -38.80186-0.209 Picture PostScript Text
3' UTR -5.2079-0.066 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR016024 - ARM-type_fold
IPR001930 - Peptidase_M1
IPR015211 - Peptidase_M1_C
IPR014782 - Peptidase_M1_N

Pfam Domains:
PF01433 - Peptidase family M1 domain

SCOP Domains:
63737 - Leukotriene A4 hydrolase N-terminal domain
55486 - Metalloproteases ("zincins"), catalytic domain

ModBase Predicted Comparative 3D Structure on Q8N6M6
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004177 aminopeptidase activity
GO:0008233 peptidase activity
GO:0008237 metallopeptidase activity
GO:0008270 zinc ion binding
GO:0016787 hydrolase activity
GO:0042277 peptide binding
GO:0046872 metal ion binding
GO:0070006 metalloaminopeptidase activity

Biological Process:
GO:0002003 angiotensin maturation
GO:0006508 proteolysis
GO:0043171 peptide catabolic process

Cellular Component:
GO:0005730 nucleolus
GO:0005737 cytoplasm
GO:0005829 cytosol


-  Descriptions from all associated GenBank mRNAs
  KJ895052 - Synthetic construct Homo sapiens clone ccsbBroadEn_04446 C9orf3 gene, encodes complete protein.
AK296782 - Homo sapiens cDNA FLJ60654 complete cds, highly similar to Aminopeptidase O (EC 3.4.11.-).
AK057450 - Homo sapiens cDNA FLJ32888 fis, clone TESTI2004295, weakly similar to AMINOPEPTIDASE B (EC 3.4.11.6).
BC029616 - Homo sapiens chromosome 9 open reading frame 3, mRNA (cDNA clone MGC:9508 IMAGE:3877098), complete cds.
AJ560639 - Homo sapiens mRNA for aminopeptidase O (APO gene).
AK027581 - Homo sapiens cDNA FLJ14675 fis, clone NT2RP2003952, weakly similar to AMINOPEPTIDASE B (EC 3.4.11.6).
BC020194 - Homo sapiens chromosome 9 open reading frame 3, mRNA (cDNA clone MGC:31762 IMAGE:5020674), complete cds.
AK296699 - Homo sapiens cDNA FLJ55832 complete cds, highly similar to Aminopeptidase O (EC 3.4.11.-).
BX648970 - Homo sapiens mRNA; cDNA DKFZp686N0860 (from clone DKFZp686N0860).
DL491396 - Novel nucleic acids.
DL490024 - Novel nucleic acids.
JD250055 - Sequence 231079 from Patent EP1572962.
AK098242 - Homo sapiens cDNA FLJ40923 fis, clone UTERU2006400.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q8N6M6 (Reactome details) participates in the following event(s):

R-HSA-8851929 AOPEP:Zn2+ hydrolyses AGT(35-41) to AGT(36-41)
R-HSA-2022377 Metabolism of Angiotensinogen to Angiotensins
R-HSA-2980736 Peptide hormone metabolism
R-HSA-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: AMPO_HUMAN, AOPEP , C9orf3, ENST00000277198.1, ENST00000277198.2, ENST00000277198.3, ENST00000277198.4, ENST00000277198.5, NM_001386073, ONPEP, Q5T9B1, Q5T9B3, Q5T9B4, Q8N6M6, Q8WUL6, Q96M23, Q96SS1, uc317jmh.1
UCSC ID: ENST00000277198.6_1
RefSeq Accession: NM_001193331.3
Protein: Q8N6M6 (aka AMPO_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.